• Title, Summary, Keyword: Harpagide

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Identification of Novel Standard Compounds for Standardization of Sinbaro3 Pharmacopuncture (신바로3 약침의 표준화를 위한 지표물질 검색)

  • Lee, Jin-Ho;Lee, Jae-Woong;Kim, Min-Jeong;Kim, Eun-Jee;Lee, In-Hee
    • Journal of Korean Medicine Rehabilitation
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    • v.25 no.2
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    • pp.65-72
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    • 2015
  • Objectives To investigate and validate potential standard compounds for standardization of Sinbaro3 pharmacopuncture prepared at OO Hospital of Korean Medicine. Methods Sinbaro3 pharmacopuncture was prepared by extraction, purification and hydrolysis of Harpagophytum procumbens, and various potential standard compounds were quantified through HPLC-UV and HPLC-MS analysis. Validation was examined by assessing specificity, linearity, precision, and accuracy. Results The retention time of harpagide and cinnamic acid were 15.2 min and 28.2 min, respectively, and both showed good linearity in analysis by concentration at 0.9999 and 0.9998, respectively. Intra-day variation of precision was 0.0015~0.0045% and 0.0058~0.1629%, while inter-day variation of precision was 0.0011~0.0243% and 0.0098~0.1629%, and that of accuracy was 99.53~99.89% and 99.50~99.91%, respectively. Conclusions Harpagide and cinnamic acid, which are hydrolyzates of harpagoside within Sinbaro3 pharmacopuncture, were both validated using HPLC-MS and HPLC-UV analysis, and Sinbaro3 pharmacopuncture contained 78.41 ug/ml harpagide, and 2.05 ug/ml cinnamic acid.

Studies on the Constituents of the Herbs of Ajuga multiflora (I)

  • Yu, Young-Jun;Do, Jae-Chul;Jung, Keun-Young;Kwon, Soon-Youl;Son, Kun-Ho
    • Korean Journal of Pharmacognosy
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    • v.29 no.2
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    • pp.75-78
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    • 1998
  • In the course of phytochemical studies for the aerial parts of Ajuga multiflora, one flavonoid and two iridoid glycosides were isolated and identified as apigenin (1), 8-O-acetylharpagide (2) and harpagide (3) on the basis of spectroscopic evidence.

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Chemical Components of the Root of Veronicastrum sibiricum Pennell (냉초(冷草)의 화학성분(化學成分) 연구(硏究))

  • Lee, Sook-Youn;Yu, Seung-Jo;Chi, Hyung-Joon
    • Korean Journal of Pharmacognosy
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    • v.18 no.3
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    • pp.168-176
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    • 1987
  • Veronicastrum sibiricum (L.) Pennell (Scrophulariaceae) is a perennial herb growing in moist land. It has been used as a korean folk medicine in treating common cold, leucorrhea, cystitis and liver damage and as cholagogue. The present study dealt with the elucidation of the chemical components. From the roots of the plant, five iridoids were isolated and identified as minecoside $(mp\;142{\sim}3^{\circ})$, 6-O-veratryl catalpol ester $(mp\;216{\sim}8^{\circ})$, catalpol $(mp\;204{\sim}6^{\circ})$, aucubin $(mp\;180{\sim}2^{\circ})$ and 6-desoxy-8-isoferuloyl harpagide $(mp\;139{\sim}41^{\circ})$. Furthermore, ${\beta}-sitosteryl-3-O-D-glucoside$, campesteryl 3-O-D-glucoside, ${\beta}-sitosterol$, campesterol, stigmasterol and mannitol were also isolated.

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Isolation of Constituents with Nitric Oxide Synthase Inhibition Activity from Phryma leptostachya var. asiatica

  • Kim, Donghwa;Lee, Sang Kook;Park, Kyoung-Sik;Kwon, Na-Yun;Park, Hee-Juhn
    • Natural Product Sciences
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    • v.25 no.1
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    • pp.34-37
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    • 2019
  • Phytochemical studies were performed to identify the active principles of Phryma leptostachya var. asiatica (Phyrymaceae) for anti-inflammation. The anti-inflammatory activity was assessed by measuring the inhibition rate on nitric oxide (NO) formation in lipopolysaccharide (LPS)-activated macrophage 264.7 cells. Of the five compounds including ursolic acid, phrymarolin I, harpagide, haedoxancoside A, and acteoside isolated from this plant, ursolic acid showed the most prominent inhibition of NO formation. Therefore, ursolic acid may be the anti-inflammatory principle of Phryma leptostachya var. asiatica.