• Title/Summary/Keyword: Hyperthermia

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Hyperthermia-induced Apoptosis is Independent upon DNA Strand Breaks in Human Lymphoid Cells

  • Jung, Hwa-Jin;Ka, Won-Hye;Hwang, Jee-Na;Seo, Young-Rok
    • The Korean Journal of Physiology and Pharmacology
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    • v.8 no.6
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    • pp.345-349
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    • 2004
  • Heat shock ($43^{\circ}C$ for 60 minutes) is sufficient to induce apoptosis in a wide number of cell lines. In this study, we asked whether DNA strand breaks are responsible for this phenomenon. Using the highly sensitive comet assay for DNA damage detection, we were unable to demonstrate DNA breaks immediately after heat shock in Raji human Iymphoid cells. It showed that DNA breaks were not necessary for hyperthermic apoptosis, since its activity is indicative of DNA lesions. Here, we present a suggestion that a protein(s) is the major target for heat shock apoptosis. We firstly found glycerol, which reportedly stabilizes protein structure, showed a protective effect in Raji cells against hyperthermic apoptosis. In addition, quercetin, which modulates transcription of the heat shock protein family members, enhanced apoptotic death induced by hyperthermia. Furthermore, Raji cells are protected by a pre-mild heat treatment prior to the killing dose of heat shock.

Development of a Direct-Vapor Heating Inhalator (증기직접가열방식의 비염치료기 개발)

  • Jeon, Chang-Wan;Kang, Taesam
    • Journal of Institute of Control, Robotics and Systems
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    • v.19 no.4
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    • pp.322-327
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    • 2013
  • Recently many people have been troubled with perennial allergic rhinitis. But there is no way to cure for root of it until now. Therefore many medical treatments to release symptoms of perennial allergic rhinitis have been researched. One of them is localized aerosol hyperthermia, which injects $42^{\circ}C{\sim}43^{\circ}C$ vapor into nasal cavity. Vapor inhalator for localized aerosol hyperthermia has been researched in foreign country. But on the other hand it has rarely been researched inside of the country. Since most clinics and hospitals have used imported vapor inhalator, it is needed to develop domestic vapor inhalator. In the paper, a superior vapor inhalator compared to former developed one is developed. The superiority of it comes from direct-vapor heating instead of water heating to control vapor temperature. The developed vapor inhalator has shorter rising time than the existing one because of direct-vapor heating. Furthermore vapor generation part and control part of the vapor inhalator developed as one piece mock-up. It enables the vapor inhalator to have smaller size. Many laboratory tests are performed and compared to existing results to prove its performance.

Cytocidal Effect of Hyperthermia on Tumor Cells in vivo (In vivo 腫瘍細胞에 미치는 溫熱處理의 細胞致死效果)

  • Kang, Man-Sik;Rhee, Jeong-Gile;Seymour H. Levitt;Chang W. Song
    • The Korean Journal of Zoology
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    • v.24 no.2
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    • pp.59-64
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    • 1981
  • The cytocidal effect of hyperthermia on subcutaneous SCK tumor cells growing in vivo was significantly greater than that on the SCK tumor cells cultured in vitro. When the tumors were left in situ after heating, the cell survival progressively decreased, and the functional intratumor vascular volume also decreased. The radiation survival curves of tumor cells heated either 30 min before or after X-irradiation in vivo were steeper than the radiation survival curves of unheated control tumors. It is concluded that the cytocidal effect of hyperthermia on tumor cells in vivo is greater than that in vitro due possibly to the intratumor environment.

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Studies on the Biological Action of Hyperthermia on Tumor Cell Mortality (1) (腫瘍細胞가 致死에 미치는 溫熱處理의 生物學的 作用에 관한 硏究(1))

  • Kang, Man-Sik;Lee, Chung-Choo
    • The Korean Journal of Zoology
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    • v.26 no.2
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    • pp.69-81
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    • 1983
  • The biological effect of hyperthermia on the SCK tumor cells in vitro were analyzed in several respects. A comparision of the survival curve of SCK tumor cells in vitro and in vivo following hyperthermia demonstrated that the cytocidal effect of heating is far greater on the cells in vivo than on the cells in vitro. The pH change in the SCK tumor upon being heated at $43.5^\\circC$ started out at 7.05 and increased to 7.18 during the first 7 min of heating and then rapidly declined to 6.67 by 30 min. Contrary to the decrease in pH in the heated tumors, the pH in the muscle increased significantly when heated to $43.5-45.0^\\circC$. Following hyperthermia at $43.5^\\circC$ for 30 min, a maximum increase in the lactic acid content in the tumor and liver was observed at 1 hr and 3 hr, respectively. The increase in the tumor was followed by a gradual decrease below the control level, whereas the increase in the liver was maintained at quite a steady level for 24 hr. The hyperthermia at $43.5^\\circC$ for 1 hr exhibited a general tendency that high molecular proteins decrease markedly, whereas most of low molecular proteins increase. The most prominent change was that the heat shock protein 70K increased significantly along with other low molecular proteins in heat shocked cells.

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Hyperthermia Properties of Fe3O4 Nanoparticle Synthesized by Hot-injection Polyol Process (Hot-injection Polyol 공정에 의해 제조된 Fe3O4 나노입자의 Hyperthermia 특성)

  • Lee, Seong Noh;Kouh, Taejoon;Shim, In-Bo;Shim, Hyun Ju
    • Journal of the Korean Magnetics Society
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    • v.24 no.2
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    • pp.51-55
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    • 2014
  • The $Fe_3O_4$ nanoparticle was synthesized by the hot-injection method while varying the injection time of the precursor solution. The crystal structure was determined to be cubic inverse spinel with space group of Fd-3m based on X-ray diffraction (XRD) measurements and the morphology of the prepared $Fe_3O_4$ nanoparticle was studied with a high-resolution transmission electron microscope (HR-TEM). When the precursor solution was injected for 0.5 min, the size of the $Fe_3O_4$ nanoparticle was 7.63 nm, while the size of the obtained particle was 21.27 nm with the injection time of 60 min. The magnetic properties of the prepared $Fe_3O_4$ nanoparticle were investigated by both vibrating sample magnetometer (VSM) and $^{57}Co$ M$\ddot{o}$ssbauer spectroscopy at various temperatures. From the hyperthermia measurement, we observed that the temperature of the $Fe_3O_4$ nanoparticle powder reached around $120^{\circ}C$ under 250 Oe at 50 kHz, when the injection time of the precursor solution was 60 min.

Development of a cylindrical ultrasound applicator for Intracavitary Hyperthermia (강내온열 치료를 위한 원통형 초음파 치료기 개발)

  • Lee, Rena J.;Hyunsuk Suh
    • Progress in Medical Physics
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    • v.13 no.2
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    • pp.79-84
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    • 2002
  • In this study, a cylindrical ultrasound applicator is developed for the treatment of vagina and rectum in combination with high dose rate brachytherapy. A cylindrical transducer (PZT-8, 1=1.5 cm, thickness=1.5mm OD=2.5 cm) was used as an energy source for induction of hyperthermia. Three single-element applicators were constructed to examine the performance of the PZT material. Vector impedance was measured to determine driving frequency. The efficiencies of the elements were determined using a radiation force technique to evaluate the feasibility of using the applicator as a hyperthermia source. A multi-element ultrasound applicator was designed using the PZT-8 material for the treatment of vagina. Results from the vector impedance measurements showed maximum magnitude at 1.78, 1.77, and 1.77 MHz for applicator 1,2, and 3, respectively. The radiation force measurements showed that the acoustic power of 40 watts was obtained in all three elements. The average efficiencies of the elements were 61.4, 65.2, and 54.0% for element 1, 2, and 3, respectively. The designed ultrasound hyperthermia applicator could be used in combination with high dose rate brachytherapy for the treatment of vagina and rectum. The use of this applicator with intracavitary brachytherapy could offer improved tumor control by increasing radiosensitiyity of the tumor.

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The Effects of Warm and Cold Stimulations on the Temperature Distribution in the Prostate (냉.온열의 반복 자극이 전립선 내부의 온도 분포에 미치는 영향)

  • 문우석;백병준;박복춘;김철생
    • Journal of Biomedical Engineering Research
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    • v.23 no.6
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    • pp.467-475
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    • 2002
  • Hyperthermia using transrectal thermal probes has been used for a noninvasive treatment of prostate diseases. However it is known that heating the rectal wall at excessively high temperature can lead to destruction of the rectal mucous membrane. and it is difficult to maintain an optimum temperature over the entire prostate. Thus, a more accurate understanding of the heat transfer mechanism between prostate and hyperthermia system is needed Numerical analysis was performed to investigate how the cold/warm stimulations on the prostate surface affect the temperature distribution in the prostate model. The general purpose software "FLUENT" was used for obtaining a finite volume solution to the unsteady conduction equation and to calculate the time-varying temperature in the prostate. Effects of the warm/cold stimulations and the stimulation frequency on the temperature distribution were simulated. and we visualized how hyperthermia affected the inside of the prostate. It was found that the effect of hyperthermia by using a typical heating method is limited due to the low thermal conductivity of the prostate. Consecutive repetitions of warm and cold stimulations were considered to provide the thermal irritations inside a prostate. The effects of temperature difference and duration of warm/cold stimulations were investigated, and basic data for the optimum period and effective patterns of stimulations were obtained. A simplified bioheat equation was also solved to describe effects of the blood flow on the blood-tissue heat transfer. The effect of blood flow was not dominant compared to that of warm/cold stimulations. These results might be used as data for design of prostate treating probe, prostatic therapy and thermal stimulation effects on the prostate.

Enhanced supply of methionine regulates protein synthesis in bovine mammary epithelial cells under hyperthermia condition

  • Zhou, Jia;Yue, Shuangming;Xue, Benchu;Wang, Zhisheng;Wang, Lizhi;Peng, Quanhui;Xue, Bai
    • Journal of Animal Science and Technology
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    • v.63 no.5
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    • pp.1126-1141
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    • 2021
  • Recent evidence has shown that methionine (Met) supplementation can improve milk protein synthesis under hyperthermia (which reduces milk production). To explore the mechanism by which milk protein synthesis is affected by Met supplementation under hyperthermia, mammary alveolar (MAC-T) cells were incubated at a hyperthermic temperature of 42℃ for 6 h in media with different concentrations of Met. While the control group (CON) contained a normal amino acid concentration profile (60 ㎍/mL of Met), the three treatment groups were supplemented with Met at concentrations of 10 ㎍/mL (MET70, 70 ㎍/mL of Met), 20 ㎍/mL (MET80, 80 ㎍/mL of Met), and 30 ㎍/mL (MET90,90 ㎍/mL of Met). Our results show that additional Met supplementation increases the mRNA and protein levels of BCL2 (B-cell lymphoma-2, an anti-apoptosis agent), and decreases the mRNA and protein levels of BAX (Bcl-2-associated X protein, a pro-apoptosis agent), especially at an additional supplementary concentration of 20 ㎍/mL (group Met80). Supplementation with higher concentrations of Met decreased the mRNA levels of Caspase-3 and Caspase-9, and increased protein levels of heat shock protein (HSP70). The total protein levels of the mechanistic target of rapamycin (mTOR) and the mTOR signalling pathway-related proteins, AKT, ribosomal protein S6 kinase B1 (RPS6KB1), and ribosomal protein S6 (RPS6), increased with increasing Met supplementation, and peaked at 80 ㎍/mL Met (group Met80). In addition, we also found that additional Met supplementation upregulated the gene expression of αS1-casein (CSN1S1), β-casein (CSN2), and the amino acid transporter genes SLC38A2, SLC38A3 which are known to be mTOR targets. Additional Met supplementation, however, had no effect on the gene expression of κ-casein (CSN3) and solute carrier family 34 member 2 (SLC34A2). Our results suggest that additional Met supplementation with 20 ㎍/mL may promote the synthesis of milk proteins in bovine mammary epithelial cells under hyperthermia by inhibiting apoptosis, activating the AKT-mTOR-RPS6KB1 signalling pathway, and regulating the entry of amino acids into these cells.