• Title, Summary, Keyword: ICR mice

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Morphological studies on kidney in cyclophosphamide-treated NOD mice (Cyclophosphamide를 투영한 NOD 마우스의 신장에 대한 형태학적 연구)

  • Lee, Joon-sup;Seong, Je-kyung
    • Korean Journal of Veterinary Research
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    • v.34 no.4
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    • pp.653-664
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    • 1994
  • This study was carried out to investigate diabetic renal changes in cyclophosphamide(CY) treated nonobese diabetic(NOD) mice and to develop animal model of diabetic human nephropathy. The 8-week-old NOD and ICR mice were injected with cyclophosphamide intraperitoneally at 200mg/kg body weight and compared the chemical effects on these mice with the non-treated NOD and ICR mice respectively. The renal glomeruli in ICR, cyclophosphamide-treated ICR(ICR+CY), NOD and cyclophosphamide-treated NOD(NOD+CY) mice were observed by the light and electron microscopes. The results obtained were summarized as follows ; 1. Spontaneous incidences of diabetes mellitus in NOD mice were significantly promoted by dosing with cyclophosphamide. 2. Glomerulohypertrophy, proliferation of mesangium, partial thickening of glomerular basement membrane, and partial fusion of pedicels of podocyte were observed in NOD mice and NOD+CY mice. These changes were not observed in both ICR mice and ICR+CY mice. 3. The diabetic nephropathy observed in NOD+CY mice was more severe than that of non-treated NOD mice.

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Acute toxicity on Samsoeum and fermented Samsoeum in ICR mice (ICR 마우스를 이용한 삼소음 및 발효삼소음의 급성독성 연구)

  • Lee, Ju-Hye;Hwang, Youn-Hwan;Lee, Ji-Hye;Yim, Nam-Hui;Cho, Won-Kyung;Ma, Jin-Yeul
    • Herbal Formula Science
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    • v.19 no.2
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    • pp.73-82
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    • 2011
  • Objectives : This study aims to investigate the acute oral toxicity and safety of Samsoeum (Shensuyin) extracts and fermented Samsoeum extracts. Methods : For that objective, we used ICR mice. ICR mice were administerd orally with dosage of 1250mg/kg, 2500mg/kg and 5000mg/kg of Samsoeum extracts and fermented Samsoeum extracts. Results : We daily examined number of deaths, clinical signs, body weights and gross findings for 2 weeks. 1. The results of acute oral toxicity using ICR mice showed that LD50 of value over 5000 mg/kg. 2. Samsoeum extracts and fermented Samsoeum extracts not affect on bodyweight gross findings of ICR mice. 3. The results of Serum chemistry analysis and Complete Blood Count(CBC) through the autopsy were showed normal range values. Conclusions : Samsoeum extracts and fermented Samsoeum extracts did not show any toxic effects in ICR mice. And oral LD50 value was over 5000mg/kg in ICR mice and it is very safe for ICR mice.

Effects of($\pm$)-camphor on Hepatic Cytochrome P450 Enzymes in Male ICR Mice (($\pm$)-Camphor가 ICR 마우스 수컷의 간 cytochrome P450 효소 활성에 미치는 영향)

  • 오은경;박형건;배기헌;최옥진;최은경;최창근;한진희;정태천
    • Environmental Analysis Health and Toxicology
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    • v.17 no.4
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    • pp.307-314
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    • 2002
  • Effects of ($\pm$)-camphor on liver cytochrome P450 enzymes were investigated in male ICR mice. Mice were treated intraperitoneally with 0, 200, 400 and 800 mg/kg of ($\pm$)-camphor in corn oil for 3 consecutive days. Twenty four hr after the final treatment, the animals were subjected to necropsy. The activities of serum aspartate aminotransferase and serum alanine aminotransferase were slightly changed by the treatment with ($\pm$)-camphor at the doses used. Administration of ($\pm$)-camphor to mice significantly induced the hepatic activities of pentoxyresorufin O-depentylase and benzyloxyresorufin O-debenzylase and weakly induced ethoxyresorufin O-deethylase in dose-dependent manners. The present results suggested that ($\pm$)-camphor might act as a relatively specific inducer of hepatic cytochrome P450 2B in male ICR mice.

Effects of Chitosan on Kidney Function in Streptozotocin-Induced Diabetic Mice

  • Sun, Kwang-Won;Kwon, Oh-Deog
    • Journal of Veterinary Clinics
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    • v.25 no.6
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    • pp.452-457
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    • 2008
  • This study examined the effects of the daily administration of high-molecular-weight (HMW) chitosan in drinking water (0.8 %) on kidney function in streptozotocin (STZ)-induced diabetic ICR mice. The HMW chitosan lowered the blood urea nitrogen (BUN), serum and urine creatinine levels, urine protein levels, and albuminuria and reduced the kidney weight in STZ-induced type 1 diabetic ICR mice. On histopathological findings, capillary loops are open, but narrowed, and the mesangial matrix enlarged in the STZ-induced diabetic ICR mice. By contrast, the capillary loops and mesangial matrix of the chitosan-treated ICR mice were nearly normal compared with the STZ-induced diabetic ICR mice.

Acute Toxicity Study on Sagunja-tang(Sijunzi-tang) in ICR Mice (ICR 마우스를 이용하여 사군자탕(四君子湯)의 급성 독성에 관한 연구)

  • Ma, Jin-Yeul;Huang, Dae-Sun;Yu, Young-Beob;Ha, Hye-Kyung;Shin, Hyun-Kyoo
    • The Journal of Korean Medicine
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    • v.28 no.2
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    • pp.200-204
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    • 2007
  • Objectives : The purpose of this study was to investigate acute toxicity of orally-treated Sagunja-tang(Sijunzi-tang) in ICR mice. Methods : In this study, we investigated the acute toxicity of water-extracted Sagunja-tang(Sijunzi-tang). Twenty-five mice completed 14 days of oral Sagunja-tang(Sijunzi-tang) at respective doses of 0 (control group), 2560, 3200, 4000 and 5000 mg/kg. Results : We observed survival rates, clinical signs of male ICR mice with acute toxicity, change of body weight and autopsy. Conclusions : Compared with the control group, we could not find any toxic alteration in anytreated groups (2560, 3200, 4000 and 5000mg/kg). LD50 of Sagunja-tang(Sijunzi-tang) was over 5000 mg/kg and it is very safe for ICR mice.

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The Long Term Effect of Buchu (Chinese chives) Diet on ROS Formation in the Liver and Skin Tissue of ICR mice (장기간의 부추식이가 ICR 마우스의 간과 피부조직의 활성산소종 생성에 미치는 영향)

  • 문갑순;이민자
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.3
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    • pp.444-449
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    • 2003
  • To investigate the long term effect of buchu (Chinese chives) diet on ROS formation in the liver and stin tissue of ICR mice, one of control, 2% or 5% buchu-added diet was fed to ICR mice for 12 months. Superoxide anion (O2ㆍ), hydrogen peroxide($H_2O$$_2$) and hydroxyl radical (ㆍOH) contents were measured in cytosol, microsome, mitochondria of liver and skin of mice, respectively. Behu diet showed a significant decrease of superoxide anion, hydrogen peroxide and hydroxyl radical contents in liver and skin tissues compared to control diet, and this effect is especially higer at 5% than at 2% buchu diet level. ICR mice showed an age-dependent increase in ROS contents, while the group fed buchu diet decreased its ROS contents significantly and ROS contents of liver appeared to be 2 fold higher than skin. The results of the present study suggest that antioxidative components and sulfur-compounds in buchu diet appear to be responsible for the inhibition of ROS formation in ICR mice.

Acute Toxicity Study of the Hwangryunhaedok-tang Extract in ICR Mice (황련해독탕(黃連解毒湯) 열수 추출물의 급성독성 연구)

  • Lee, Ji-Hye;Eum, Hyun-Ae;Chung, Tae-Ho;Lee, Yoon-Hee;Um, Young-Ran;Yim, Nam-Hui;Kim, Dong-Seon;Ma, Jin-Yeul
    • Herbal Formula Science
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    • v.18 no.2
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    • pp.159-166
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    • 2010
  • Objective : The objective of this study was to evaluate the single dose oral toxicity of Hwangryundaedok-tang extract in ICR mice. Methods : 0(control group), 1250, 2500 and 5000 mg/kg of Hwangryundaedok-tang extracts were orally administered to 20 male and 20 female ICR mice. After single oral administration of Hwangryundaedok-tang extract to ICR mice, we observed number of the death, clinical signs, changes of body weights for 14 days. After 14 day of Hwangryundaedok-tang extract administration, all mice were sacrificed and major organs were observed. Results : Compared with the control group, we could not find any toxic signs in the mortalities, clinical signs, body weight changes, necropsy findings and hematological values in all treated groups(1250, 2500 and 5000 mg/kg). Conclusions : $LD_{50}$ value of Hwangryundaedok-tang extracts may be over 5000 mg/kg and it may have no side toxic effect to ICR mice.

Susceptibility of several strains of mice to Echinostoma hortense infection

  • Lee, Kyu-Jae;Park, Seung-Kyu;Im, Jee-Aee;Kim, Soo-Kie;Kim, Geun-Ha;Kim, Gwang-Young;Yang, Eun-Ju;Ryang, Yong-Suk
    • The Korean Journal of Parasitology
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    • v.42 no.2
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    • pp.51-56
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    • 2004
  • Susceptibilities of 5 different mice strains, including C3H/HeN, BALB/c, C57BL6, FvB and ICR, to Echinostoma hortense infection, was evaluated. The worm expulsion rate, worm size and egg production were observed from 1 to 8 weeks after infection with 30 metacercariae. C3H/HeN and ICR mice showed the highest worm maturation rates. The worm recovery rate and the number of eggs per gram (EPG) of feces was also higher in C3H/HeN and ICR mice than in BALB/c, C57BL6, and FvB mice. It is suggested that E. hortense is highly infectious to ICR and C3H/HeN mice, but not to the other strains of mice. Based on the results obtained, we believe that the susceptibility of different mouse strains to E. hortense infection is dependent on the genetic and immunologic back-ground of mice.

Acute Intravenous and Oral Toxicity of DWC-751 in Rats and Mice (랫드 및 마우스에서 DWC-751의 급성정맥 및 경구 독성시험)

  • 김재현;박창원;강진석;유영효;박정식
    • Toxicological Research
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    • v.11 no.1
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    • pp.109-116
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    • 1995
  • Single intravenous and oral administration to SD rats and ICR mice of both sexes were performed to investigate the acute toxicity of DWC-751, a new parenteral cephalosporin. $LD_50$ values for ICR mice and SD rats administered intravenously with DWC-751 were as follows; 1151.1 mg/kg (male SD rat), 1183.5 mg/kg (female SD rat), 2698.1 mg/kg (male ICR mouse), 2833.0 mg/kg (female ICR mouse). It is suggested that $LD_50$ values in rats and mice of both sexes would be 5000 mg/kg in oral route. Major general symptoms induced by injection intravenously with DWC-751 are decreased motor activity, increased respiratory rate, tremor and convulsion. In oral route, piloerection and soft stool are observed to 4 day after administration. No significant body weight changes were observed at any level in the groups administered with DWC-751. The gross finding of rats administered intravenously was observed cecum distension.

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Comparison of the anesthetic effects of 2,2,2-tribromoethanol on ICR mice derived from three different sources

  • Lee, Mi Ree;Suh, Hye Rin;Kim, Myeong Whan;Cho, Joon Young;Song, Hyun Keun;Jung, Young Suk;Hwang, Dae Youn;Kim, Kil Soo
    • Laboraroty Animal Research
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    • v.34 no.4
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    • pp.270-278
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    • 2018
  • This study was conducted to compare the anesthetic effects of 2,2,2-tribromoethanol (TBE, $Avertin^{(R)}$) in ICR mice obtained from three different sources. TBE (2.5%) was intraperitoneally injected at three doses: high-dose group (500 mg/kg), intermediate-dose group (250 mg/kg), and low-dose group (125 mg/kg). Anesthesia time, recovery time, end-tidal peak $CO_2$ ($ETCO_2$), mean arterial blood pressure, heart rate, oxygen saturation ($SpO_2$), body temperature, pH, $PCO_2$, and $PO_2$ of the arterial blood were measured. Stable anesthesia was induced by all doses of TBE and the anesthesia time was maintained exhibited dose dependency. No significant differences in anesthetic duration were found among the three different strains. However, the anesthesia time was longer in female than in male mice, and the duration of anesthesia was significantly longer in female than in male mice in the high-dose group. The recovery time was significantly longer for female than male mice in the intermediate- and high-dose groups. In the ICR strains tested, there were no significant differences in the mean arterial blood pressure, $SPO_2$, arterial blood $PCO_2$, and $PO_2$, which decreased after TBE anesthesia, or in heart rate and $ETCO_2$, which increased after TBE anesthesia. In addition, body temperature, blood biochemical markers, and histopathological changes of the liver, kidney, and lung were not significantly changed by TBE anesthesia. These results suggested that ICR mice from different sources exhibited similar overall responses to a single exposure to TBE anesthesia. In conclusion, TBE is a useful drug that can induce similar anesthetic effects in three different strains of ICR mice.