• Title, Summary, Keyword: In-situ investigation

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Detection of Cytomegalovirus in Atherosclerotic Aorta and Coronary Artery by In Situ Hybridization and PCR

  • Eom, Yong-Bin;Kwon, Tae-Jung;Lee, Sang-Yong;Lee, Won-Tae;Kim, Jong-Bae
    • Biomedical Science Letters
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    • v.8 no.4
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    • pp.257-268
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    • 2002
  • Chronic infection and inflammation have recently been implicated as important etiologic agents of atherosclerosis. Several agents have been suggested as possible candidates including cytomegalovirus (CMV), herpes simplex vims type 1 (HSV-1), Epstein-Barr virus (EBV), Chlamydia pneumoniae, and Helicobacter pylori. We evaluated the relationship between cytornegalovirus infection and atherosclerosis by in situ hybridization and polymerase chain reaction (PCR). We examined 23 subjects with atherosclerosis and 10 matched control subjects without atherosclerosis. CMV was detected by in situ hybridization in 60.9% (14/23) of aorta and 42.9% (9/21) of coronary arteries in subjects with atherosclerosis. It was also detected by PCR in 65.2% (15/23) of aorta and 52.4% (11/21) of coronary arteries. CMV was detected on areas showing early or advanced atheromatous changes. Cells morphologically identical to smooth muscle cells, endothelial cells, lymphocytes, fibroblasts, and Schwann cells were positively reacted with the CMV probe. However. none of the cells to which the probe hybridized contained inclusion bodies, thus strongly suggesting that the arterial wall may be a site of CMV latency. This result Indicates that CMV may potentially play a direct or indirect role in the pathogenesis of human atherosclerosis.

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Technical Investigation into the In-situ Electron Backscatter Diffraction Analysis for the Recrystallization Study on Extra Low Carbon Steels

  • Kim, Ju-Heon;Kim, Dong-Ik;Kim, Jong Seok;Choi, Shi-Hoon;Yi, Kyung-Woo;Oh, Kyu Hwan
    • Applied Microscopy
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    • v.43 no.2
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    • pp.88-97
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    • 2013
  • Technical investigation to figure out the problems arising during in-situ heating electron backscatter diffraction (EBSD) analysis inside scanning electron microscopy (SEM) was carried out. EBSD patterns were successfully acquired up to $830^{\circ}C$ without degradation of EBSD pattern quality in steels. Several technical problems such as image drift and surface microstructure pinning were taking place during in-situ experiments. Image drift problem was successfully prevented in constant current supplying mode. It was revealed that the surface pinning problem was resulted from the $TiO_2$ oxide particle formation during heating inside SEM chamber. Surface pinning phenomenon was fairly reduced by additional platinum and carbon multi-layer coating before in-situ heating experiment, furthermore was perfectly prevented by improvement of vacuum level of SEM chamber via leakage control. Plane view in-situ observation provides better understanding on the overall feature of recrystallization phenomena and cross sectional in-situ observation provides clearer understanding on the recrystallization mechanism.

A Study on the Developement of Korean Driving Cone Penetrometer Test(DCPT) Method (한국형타격콘관입시험법의 개발에 관한 연구)

  • Jung, Sung-Min;Kwon, Oh-Sung;Lee, Jong-Sung;Lee, Min-Hee;Choi, Yong-Kyu
    • Journal of the Korean Geotechnical Society
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    • v.27 no.8
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    • pp.17-30
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    • 2011
  • A variety of in-situ geotechnical investigation methods are currently used to measure the properties of each site, but in-situ tests for "Intermediate Geomaterial (IGM)", which is the transitional geomaterial between soil and rock, have only limited application. In the United States, "The Texas Cone Penetrometer Test (TCPT)", which is the geotechnical investigation technology for IGM, is utilized to create foundation designs. This paper introduces "The Driving Cone Penetrometer Test (DCPT)", which can be performed using general geotechnical investigation equipment and also analyzes the correlation between various in-situ geotechnical investigation methods by applying DCPT on the ground. The results showed that the correlation between the driving cone penetrometer test (DCPT) and standard penetration test (SPT) was quite high. Additionally, the scope of DCPT properties was wide, depending on soil types.

A Study on the Applicability of Resistivity Cone for Contaminant Investigation in Farm Land (농경지 오염탐사를 위한 비저항콘의 적용성에 관한 연구)

  • Yu, Chan;Yoon, Chun-Gyeong
    • Journal of Korean Society of Rural Planning
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    • v.5 no.1
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    • pp.45-55
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    • 1999
  • The applicability of resistivity cone for contaminant investigation in farm land was examined in laboratory and in-situ. To verify the resistivity variations in sand with contaminants, laboratory experiment used soil resistivity test box was performed. Heavy metal and others ions were appeared steep variations in low concentration. Pilot test results were the same as laboratory test results. In the in-situ test used resistivity cone, resistivities of waste landfill layer were low than the common soil layers and resistivities had been difference with concentration of contaminant components. Therefore contaminant investigation in farm land using resistivity cone was expected very effective.

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Geotechnical parameters from pressuremeter tests for MRT Blue Line extension in Bangkok

  • Likitlersuang, Suched;Surarak, Chanaton;Wanatowski, Dariusz;Oh, Erwin;Balasubramaniam, Arumugam
    • Geomechanics and Engineering
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    • v.5 no.2
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    • pp.99-118
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    • 2013
  • Construction of the extension project of the Bangkok MRT Blue Line underground railway was recently started in 2011. The construction of approximately 5 km long underground tunnel and 4 deep excavations of underground station are considered to be the most important geotechnical works. The pressuremeter was selected as a high-quality in situ testing of the soil to evaluate design parameters for the project. In addition, other field and laboratory tests such as vane shear and $CK_0U$ triaxial tests were included in the investigation programme. This paper aims to present the ground conditions encountered along the MRT Blue Line extension project as well as the site investigation and interpretation techniques with particular focus on the pressuremeter tests. The results are also compared with the pressuremeter investigation from the previous Bangkok MRT project.

A Study on the Applicable Methods of Investigation for Cutting Slope Design (깍기 비탈면 설계에서의 지질조사 기법의 적용성 분석)

  • Lee, Su-Gon;Choi, Kyeong-Chim;Kim, Jae-Heun
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.9 no.1
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    • pp.100-111
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    • 2006
  • This study was carried out to increase the efficiency of site investigation through the evaluation of the applicable methods of investigation for cutting slope design. In an effort to find out the applicability of the method employed during the slope investigation, some tests were carried out on 6 subjects i.e location of weak zones, characteristics of discontinuities, distribution of strata, in situ tests for geo-technical properties, laboratory test and estimation of weathering. The method was highly applicable and produced expected results during the test of weak zones, discontinuities and distribution of strata. In order to apply the method to in- situ test for geo-technical properties and laboratory test on soil and rock slope, a statistic analysis of the existing data were required in advance. However its design applicability on rock slope was good although actual cases of application were not many due to limitation of the cases for investigation. The method was frequently referenced but not actually applied for anti seismic design test and estimation of weathering.

Investigation of the Performance Characteristics of an In-Situ Particle Monitor at Low Pressures Using Aerodynamic Lenses (저압상태에서 공기역학적 렌즈를 이용한 In-Situ Particle Monitor의 성능특성 분석)

  • Bae, Gwi-Nam
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.24 no.10
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    • pp.1359-1367
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    • 2000
  • In-situ particle monitors(ISPMs) are widely used for monitoring contaminant particles in vacuum-based semiconductor manufacturing equipment. In the present research, the performance of a Particle Measuring Systems(PMS) Vaculaz-2 ISPM at low pressures has been studied. We generated the uniform sized methylene blue particle beams using three identical aerodynamic lenses in the center of the vacuum line, and measured the detection efficiency of the ISPM. The effects of particle size, particle concentration, mass flow rate, system pressure, and arrangement of aerodynamic lenses on the detection efficiency of the ISPM were examined. Results show that the detection efficiency of the ISPM greatly depends on the mass flow rate, and the particle Stokes number. We also found that the optimum Stokes number ranges from 0.4 to 1.9 for the experimental conditions.

Investigation of pretilt generation by UV light irradiation during imidization of polyimide (폴리이미드 소성 시에 UV 광조사를 이용한 프리틸트 발생에 대한 연구)

  • 서대식;김형규
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.13 no.1
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    • pp.75-79
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    • 2000
  • In this study, we investigated the pretilt angle generation and liquid crystal (LC) alignment by ultravi-olet (UV) light irradiation during imidization of polymide. The generated pretilt angle of nematic (N) LC by using the in-situ photo-alignment method was smaller than that of the conventional UV photo-alignment method. Also, generated pretilt angle of NLC tends to increase by annealing. In case of using the polymer(AL-3046), we found that the in-situ Uv photo-alignment method has higher thermal stability of LC alignment, but it has a disadvantage to control pretilt angle.

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Nondestructive Characterization and In-situ Monitoring of Corrosion Degradation by Backward Radiated Ultrasound

  • Song, Sung-Jin;Kim, Young H.;Bae, Dong-Ho;Kwon, Sung D.
    • Corrosion Science and Technology
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    • v.4 no.3
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    • pp.114-119
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    • 2005
  • Since the degradation caused by corrosion is restricted to the surface of materials, conventional ultrasonic nondestructive evaluation methods based on ultrasonic bulk waves are not applicable to characterization of the corrosion degradation. To take care of this difficulty, a new nondestructive evaluation method that uses ultrasonic backward radiation has been proposed recently. This paper explores the potential of this newly developed method for nondestructive characterization and in-situ monitoring of corrosion degradation. Specifically, backward radiated ultrasounds from aged thermo-mechanically controlled process (TMCP) steel specimens by corrosion fatigue were measured and their characteristics were correlated to those of the aged specimens. The excellent correlation observed in the present study demonstrates the high potential of the backward radiated ultrasound as an effective tool for nondestructive characterization of corrosion degradation. In addition, the potential of the backward radiated ultrasound to in-situ monitoring of corrosion degradation is under current investigation.