• Title, Summary, Keyword: Indirect detection

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Development of an Indirect Enzyme-Linked Immunosorbent assay for Rapid Detection of Adulteration of Food Allergen Mackerel in Processed Marine Foods (수산가공식품 중 알레르겐 고등어 혼입여부 신속 검출을 위한 간접효소면역분석법의 개발)

  • Lee, Jeong-Eun;Kim, Ah-Yoon;Kim, Sol-A;Kim, Hyo-In;Park, Ji-Hye;Shim, Won-Bo
    • Journal of Food Hygiene and Safety
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    • v.33 no.3
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    • pp.185-192
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    • 2018
  • The purpose of this study was to develop an indirect enzyme-linked immunosorbent assay (indirect ELISA) based on a monoclonal antibody (MAb) that is specific to mackerel thermal stable-soluble protein (TSSP), that can be used for the rapid detection of mackerel in processed marine foods. Among the four MAbs (3A5-1, 2, 9, and 12) developed in previous studies, the 3A5-2 MAb that showed high specificity and sensitivity were selected and used to develop the indirect ELISA method. The detection range of the indirect ELISA was 0.02%-0.001% and the detection limit of 0.001% was shown. No cross-reaction to other marine products and food ingredients was observed by the indirect ELISA. Processed marine foods containing mackerel with ${\geq}0.3$ O.D. value at 405 nm were estimated as positive samples by the indirect ELISA. Therefore, the indirect ELISA can be used as a rapid and sensitive method to identify mackerel authenticity and adulteration in processed marine foods.

Indirect Detection of Rotor Position of Switched Reluctance Motor Based on Flux Linkage Analytic Model

  • Zhou, Yongqin;Hu, Bo;Wang, Hang;Jin, Ningzhi;Zhou, Meilan
    • Journal of Electrical Engineering and Technology
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    • v.13 no.2
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    • pp.829-837
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    • 2018
  • In this paper, a flux linkage model based on four magnetization curves fitting is proposed for three-phase 12/8 switched reluctance motor (SRM), with the analysis of the basic principle of flux detection method and function analysis method. In the model, the single value function mapping relationship between position angle and flux is established, which can achieve a direct estimation of rotor position. The realization scheme of SRM indirect position detection system is presented. It is proved by simulation and experiment that the proposed scheme is suitable for rotor position detection of SRM, and has high accuracy of position estimation.

Procedures for Detecting Multiple Outliers in Linear Regression Using R

  • Kwon, Soon-Sun;Lee, Gwi-Hyun;Park, Sung-Hyun
    • Proceedings of the Korean Statistical Society Conference
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    • pp.13-17
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    • 2005
  • In recent years, many people use R as a statistics system. R is frequently updated by many R project teams. We are interested in the method of multiple outlier detection and know that R is not supplied the method of multiple outlier detection. In this talk, we review these procedures for detecting multiple outliers and provide more efficient procedures combined with direct methods and indirect methods using R.

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Detection of Edwardsiella tarda, the Pathogenic Bacteria in Freshwater Fishes by Means of the Indirect Fluorescent Antibody Technique (간접형광항체법을 이용한 담수양식어의 병원균 Edwardsiella tarda의 검출)

  • 류해진;조우영;이청산;허강준
    • Korean Journal of Veterinary Service
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    • v.16 no.2
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    • pp.111-119
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    • 1993
  • In this study, we carried out the rapid diagnostic system based on indirect fluorescent anti-body technique (IFAT) for detection of bacterial diseases in cultured freshwater fishes. 1. When the fishes were tested with graded dilution of Edwardsiella tarda FPC 470 bacteria detection from ten fishes Injected with $4.1{\times}10^3$colony forming unit(CFU) /ml, all of them were detected by IFAT but only two fishes were recognizable by the culture method in the tested fishes injected with $4.1{\times}10^3$CFU /ml. 2. The bacteria E. tarda could be detected by IFAT method from 1 to 48hrs after Injection in the tissues tested such as kidney, liver and spleen of the fishes, whereas detection by culture method could be recognized from 1 to 48hrs after injection In the kidney and spleen but it was not possible from preinjection to 1 hr in the liver. 3. Thus, IFAT proved to be more useful technique than plate culture method in the diagnosis of Edwardsiellosis in the freshwater fishes.

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Intelligent Internal Stealthy Attack and its Countermeasure for Multicast Routing Protocol in MANET

  • Arthur, Menaka Pushpa;Kannan, Kathiravan
    • ETRI Journal
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    • v.37 no.6
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    • pp.1108-1119
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    • 2015
  • Multicast communication of mobile ad hoc networks is vulnerable to internal attacks due to its routing structure and high scalability of its participants. Though existing intrusion detection systems (IDSs) act smartly to defend against attack strategies, adversaries also accordingly update their attacking plans intelligently so as to intervene in successful defending schemes. In our work, we present a novel indirect internal stealthy attack on a tree-based multicast routing protocol. Such an indirect stealthy attack intelligently makes neighbor nodes drop their routing-layer unicast control packets instead of processing or forwarding them. The adversary targets the collision avoidance mechanism of the Medium Access Control (MAC) protocol to indirectly affect the routing layer process. Simulation results show the success of this attacking strategy over the existing "stealthy attack in wireless ad hoc networks: detection and countermeasure (SADEC)" detection system. We design a cross-layer automata-based stealthy attack on multicast routing protocols (SAMRP) attacker detection system to identify and isolate the proposed attacker. NS-2 simulation and analytical results show the efficient performance, against an indirect internal stealthy attack, of SAMRP over the existing SADEC and BLM attacker detection systems.

Analysis of Anions by Indirect Photometric Detection (I) (간접 분광 검출법에 의한 음이온의 분석(I))

  • Park, Man-Ki;Kim, Bak-Kwang;Park, Jeong-Hill;Kim, Kyoung-Ho;Lee, Mi-Yung;Jung, Jae-Eun
    • YAKHAK HOEJI
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    • v.34 no.3
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    • pp.215-218
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    • 1990
  • An ion chromatographic method based on indirect photometric detection of UV transparent anions was developed. Separation of anion was accomplished on strong anion exchange column (Waters SAX) using UV detector at 254 nm. Among examined UV-active additives (Dns-H, Dns-glu, Dnsser, Dns-val), Dns-glu showed the highest sensitivity. Studies on the effects of the pH and ionic strength of eluent revealed that the increase of pH and ionic strength of the eluent decreased capacity factor. The best eluent for the separation of acetate, fluoride, chloride, nitrate and bromide was $1\;{\times}\;10^{-4}M$ Dns-glu in 5 mM phosphate buffer (pH 6.30). The detection limit of chloride ion was 2.1 ng in this condition.

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THE EFFECT OF SURFACE ROUGHNESS OF CSI(TL) MICRO-COLUMNS ON THE RESOLUTION OF THE X-RAY IMAGE; OPTICAL SIMULATION STUDY

  • Kim, Hyun-Ki;Bae, Jun-Hyung;Cha, Bo-Kyung;Jeon, Ho-Sang;Kim, Jong-Yul;Kim, Chan-Kyu;Cho, Gyu-Seong
    • Journal of Radiation Protection and Research
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    • v.34 no.1
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    • pp.25-30
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    • 2009
  • Micro-columnar CsI(Tl) is the most popular scintillator material which is used for many indirect digital X-ray imaging detectors. The light scattering at the surface of micro-columnar CsI(Tl) scintillator was studied to find the correlation between the surface roughness and the resultant image resolution of indirect X-ray imaging detectors. Using a commercially available optical simulation program, Light Tools, MTF (Modulation Transfer Function) curves of the CsI(Tl) film thermally evaporated on glass substrate with different thickness were calculated and compared with the experimental estimation of MTF values by the edge X-ray image method and CCD camera. It was found that the standard deviation value of Gaussian scattering model which is determined by the surface roughness of micro-columns could certainly change the MTF value of image sensors. This model and calculation methodology will be beneficial to estimate the overall performance of indirect X-ray imaging system with CsI(Tl) scintillator film for optimum design depending on its application.

A Studies on Indirect Photometric Detection of Aliphatic Compounds by High-Performance Liquid Chromatography (고성능 액체 크로마토그래피에 의한 지방족 화합물의 간접 분광광도 측정에 관한 연구)

  • Sam-Woo Kang;Jeon-Tag Kang
    • Journal of the Korean Chemical Society
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    • v.33 no.4
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    • pp.406-412
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    • 1989
  • Indirect photometric detection of aliphatic compounds such as alcohols, ketones and esters was investigated in high-performance liquid chromatography, These samples possessing nonchromophore or weak chromophore could be indirectly detected by a UV detector with addition of UV absorbing, p-chlorophenol or p-nitrophenol in mobile phase as detection reagent, and submicrogram detection limits were achieved. Some mixtures of samples were also able to be separated and quantitated with good resolution and comparatively high sensitivity under optimum conditions.

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Anti-islanding Method by Harmonic Injection for Utility Interactive Inverter with Critical Load (중요부하를 갖는 계통연계형 인버터의 고조파주입에 의한 단독운전방지 기법)

  • Oh, Hyeong-Min;Choi, Se-Wan;Kim, Tae-Hee;Lee, Gi-Pung;Lee, Tae-Won
    • The Transactions of the Korean Institute of Power Electronics
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    • v.17 no.4
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    • pp.315-321
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    • 2012
  • The utility-interactive inverter with critical loads should supply continuous and stable voltage to critical loads even during the grid fault. The conventional control method which performs current control for grid-connected mode and voltage control for stand-alone mode undergoes the critical load voltage variation during grid fault. The critical load voltage may have large transient when the inverter performs mode transfer after the islanding detection. Recently, the indirect current control method which does not have the transient state during not only islanding detection but also the mode transfer has been proposed. However, since the voltage control is maintained even during the grid-connected mode it is difficult to detect the islanding. This paper proposes an active anti-islanding method suitable for the indirect current control method which does not have NDZ(Non-Detection Zone).

Establishment of Indirect Competitive ELISA for the Detection of Zearalenone Produced by Fusarium sp. (Fusarium속이 생성하는 zearalenone 측정을 위한 Indirect Competitive ELISA의 확립)

  • 강성조;정덕화
    • Journal of Food Hygiene and Safety
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    • v.13 no.4
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    • pp.419-424
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    • 1998
  • An enzyme-linked immunosorbent assay (ELISA) was established for the detection of zearalenone by using monoclonal antibodies produced by Z-M-26 hybridoma cells when injected into a mouse and zearalenone-oxime-OV A conjugate. Zearalenone-oxime-OV A conjugates were diluted with carbonyl buffer, coated to 96 well microtiter plates at $4^{\circ}C$ overnight and blocked with 1% BSA overnight. One thousand times diluted antibody solution together with standard zearalenone or sample was added to 96-well microtiter plates and stood overnight. A secondary antibody conjugated with HRP was added and an hour later, enzyme substrate (TMBZ) solution was added for color develpment. Mter 30 minutes, coloring reaction was terminated by adding 2 N $H_2S0_4$ and the O.D. was measured at 450 nm. Detection range of this method was about 0.1~100 ppb. The established indirect competitive ELISA method was suitable for a rapid and effective analysis of zearalenone in agricultural products.

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