• Title, Summary, Keyword: Insecticide susceptibility

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Insecticide Susceptibility of Field-Collected Populations of the Spiraea Aphid, Aphis citricola(van der Goot)(Hemiptera: Aphididae) in Apple Orchards (사과 과수원에서 조팝나무진딧물의 살충제 감수성)

  • ;;Naoki Motoyama
    • Korean journal of applied entomology
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    • v.32 no.3
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    • pp.259-264
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    • 1993
  • The experiment was carried out to investigate Insecticide susceptibility of the spiraea aphid (Aphis citricola) in apple orchards In Korea, using dipping method. Although insecticIde susceptibility vaned with local and seasonal populations, the susceptibility to demeLon S-rnethyl and phosphamidons was different from that Lo the other insecticides. The LCso values were 10 ppm for deltamethrin and chloropyrifos, 103 ~ 629 ppm for demeton S-methy1, acephate, phosphamidon, monocrotophos and vamldothion, 12,200 ppm for EPN, and 1,745 ppm for pirimicarb. 'When Insecticide susceptibility was compared between the S-clone selected from a population on the host plant spiraea and the R-clone selected from a population on the apple tree, the resistance ratio as expressed by RIS ratio of $LC_{50}$ was 78 for phosphamidon and 546 for pirimicarb. Esterase zymogram determined by the agar gel electrophoresIs revealed a significant difference between the clones. The activity of the E2, E5, E6 and E7 of the R-clone was higher than that of the S-clone, It is suggesLed that the Increased esterase activity may be involved in the mechanism of insecticide resistance in the spiraea aphid, although the involvement of other factor(s) may not be ruled out.

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Monitoring on Insecticide resistance of major insect pests in paddy field (주요 벼 해충에 대한 약제저항성 모니터링)

  • Lee, Si-Woo;Choi, Byeong-Ryeol;Park, Hyung-Man;Yoo, Jai-Ki
    • The Korean Journal of Pesticide Science
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    • v.9 no.4
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    • pp.365-373
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    • 2005
  • This study was carried out for looking into the status of susceptibility of pest insects to insecticides. Each insect Brown planthopper(BPH), Green leaf hopper(GLH), Smaller brown plant hopper(SBPH), Rice water weevil(RWW), were captured at various areas where the host crops were being cultivated and the susceptibility level of each pest insect was investigated. The susceptibility of each pest insect varied by insect species and areas where they were caught. BPHs kept higher level of susceptibility comparing to susceptible reference strain except to most of tested insecticides except imidacloprid (Resistance ratio was 68). The susceptibilities of GLH and SBPH to most of insecticides for their control did not developed markedly since 1976 except fipronil and imidacloprid which is widely used for WRR control. The insecticides used for control of WRR were very effective even at the concentration of one fourth of recommending concentration, but in 2000 suwon strain of WRR showed markedly reduction of susceptibility to carbofuran.

Analysis of Pyrethroid Resistance Allele in Malaria Vector Anopheles sinensis from Malaria High-risk Area (말라리아 위험지역에서 채집된 말라리아 매개모기 Anopheles sinensis의 피레스로이드계 저항성 대립형질 분석)

  • Choi, Kwang Shik;Lee, Seung-Yeol;Hwang, Do-Un;Kim, Heung-Chul;Chang, Kyu-Sik;Jung, Hee-Young
    • The Korean Journal of Pesticide Science
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    • v.20 no.4
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    • pp.286-292
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    • 2016
  • Malaria is mainly transmitted by Anopheles sinensis which is dominant species in malaria high-risk area, northern part of Gyeonggi province in Korea. Pyrethroid insecticide is used for malaria vector, An. sinensis in Korea and the previous investigation consistently reported insecticide resistance from the vector. This study investigated insecticide susceptible and resistant alleles from An. sinensis and the status of malaria vector control in malaria high-risk area. For the study, An. sinensis collected from Paju, Gimpo and Ganghwa were sequenced for kdr detection. In Paju, there was no homozygous susceptibility and all of tested samples had homozygous or heterozygous resistance. There were 6.7% for susceptible homozygosity and 93.3% for resistant homozygosity or heterozygosity in Gimpo. Furthermore, the percentages of homozygous susceptibility and homozygous or heterozygous resistance in Ganghwa were 5.7% and 94.3% respectively. The results showed that the frequency of the insecticide resistance from An. sinensis in malaria high-risk area were increased much more than the previous investigation. Hence, this study suggests that malaria vector control programs should have to be prepared for the management of pyrethroid insecticide resistance.

Strategy for Insecticide Resistance Management Approach to IPM

  • Motoyama, Naoki;Dauterman, W.C.
    • Korean journal of applied entomology
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    • v.31 no.3
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    • pp.314-327
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    • 1992
  • Insecticide resistance is a serious is a serious threat to IPM, resulting in various adverse effects not to mention the loss of yield in agriculture. One approach to counter the problem is the disruption of resistance mechanisms. This can be achieved by (1) compounds which show a negative correlation with resistance at the site of action, (2) specific metabolic inhibitors which serve as synergists, or (3) a certain combination of two insecticides producing a joint action. This approach, however, requires certain precautions for the side effects may cause an increase in toxicity to mammals. Owing to the recent advances in theoretical studies on resistance management employing computer simulation and mathematical models, a few principles to reduce the risk of development of resistance have been clarified. They are helpful in designing operational strategies with regard to, for instance, insecticide doses to be applied, mode of application, and choice and nature of the insecticide(s) to be used. For restoration of insecticide susceptibility of a resistant population, reintroduction of susceptible individuals to the resistant population is feasible when certain conditions are met. Natural enemies which developed resistance to insecticides can be an important component of IPM as has been shown in the pest management in apple orchards. After all, the implementation of a successful resistance management program depends upon cooperation between different sigments of the agricutural community. Although resistance is a preadaptive phenomenon, in some cases spontaneous loss of resistance does occur without contamination by susceptible individuals. The instability of resistance in these insects implies the possible existence of a switch machanism controlling the expression of resistance gene(s). Elucidation of such a mechanism may eventually provide us with a new technical approach with which we can combat the problem of insecticide resistance.

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Sound Stress Induces Developmental Alterations and Enhances Insecticide Susceptibility in the Green Peach Aphid, Myzus persicae (스트레스 음파 처리에 따른 복숭아혹진딧물(Myzus persicae)의 발육 교란 및 살충 효과)

  • Seok, Jeong-Gyun;Kang, Taek-Jun;Kim, Yong-Gyun
    • The Korean Journal of Pesticide Science
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    • v.14 no.4
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    • pp.415-420
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    • 2010
  • This study investigated effects of different sound frequencies on development and insecticide susceptibility of the green peach aphid, Myzus persicae. Under a constant sound intensity (95 dB), different frequency (0-5,000 Hz) sounds were treated on entire developmental period of the aphids. With increase of sound frequencies, nymphal to adult development of the aphids showed significant retardation and the females exhibited a significant decrease in fecundity. The greatest disrupting effects were induced by treatment of 5,000 Hz, which also caused marked change in protein expression of the aphids analysed by two dimensional electrophoresis. Sound frequencies above 2,000 Hz significantly increased susceptibility of the aphids against imidacloprid. This study clearly suggest that there is a stress sound, which can be sensed by M. persicae and induces its physiological alteration.

Effect on Insecticide Susceptibility of Lissorhoptrus oryzophilus Fed on Carotenoid-Biofortified Rice Variety (비타민 A 강화벼 급이가 벼물바구미(Lissorhoptrus oryzophilus)의 살충제 감수성에 미치는 영향)

  • Oh, Sung-Dug;Lee, Ki-Jong;Park, Soo-Yun;Ryu, Tae-Hun;Kim, Jae-Kwang;Sohn, Soo-In;Kim, Jin-Seo;Ha, Sun-Hwa;Park, Jong-Sug;Ahn, Byung-Ohg;Cho, Hyun-Suk;Suh, Sang-Jae
    • Korean Journal of Environmental Agriculture
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    • v.31 no.3
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    • pp.286-292
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    • 2012
  • BACKGROUND: The carotenoid-biofortified (PAC) rice was generated by inserting phytoene synthase (Psy) and carotene desaturase (Crtl) genes isolated from Capsicum annuum cv. Nockwang and Pantoea ananatis into the genome of a conventional variety of rice (Nakdongbyeo). In our present study, we studied the effects on insecticide susceptibility of Rice Water Weevil (Lissorhoptrus oryzophilus). METHODS AND RESULTS: The L. oryzophilus were fed on carotenoid-biofortified (PAC) rice and its near non-genetically modified (GM) counterparts (Nakdongbyeo) under $25{\pm}1^{\circ}C$, humidity of $60{\pm}5%$, and photoperiod 16L:8D for more than 60 days. Ten adults were soaked in the Clothianidin SC solution for 5 second in different concentrations, and were detected the mortalities after 24, 48 and 72 hours respectively. Every experiment was conducted with three replications. The cumulative mortalities of L. oryzophilus exposed to Clothianidin SC were similar between two types of feed administration. CONCLUSION: The results of this study suggested that carotenoid-biofortified rice might not affect the insecticide susceptibilities of Lissorhoptrus oryzophilus.

Ecological Characteristics and Insecticidal Susceptibility of Sycamore Lace Bug, Corythucha ciliata Say (Hemiptera: Tingidae) (버즘나무방패벌레 (Corythucha ciliata Say)의 생태적 특성 및 살충제감수성)

  • Song, Cheol;Cho, Kwang-Yun
    • Journal of Life Science
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    • v.10 no.2
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    • pp.164-168
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    • 2000
  • To understand the biological characteristics of Corythucha ciliata occurring in Korea, developmental periods and its susceptibility to several insecticides were examined under growth chamber condition at $25^{\circ}C$. It took 11.1 day from egg to hatch. And duration of each stage up to the 5th nymph after hatching was 4.0, 2.1, 2.0, 2.9, and 4.0 days, respectively. Total duration from egg to adult was 26.1 days, preoviposition period was 12.2 days, and average number of eggs laid by a female was 83.0. Sex ratio of female was 51.6% and lifespan of female adult was 43.2% days. LC50 value of deltamethrin and esfenvalerate were 0.8 and 0.9 ppm, respectively. Insecticidal effects were better in pyrethroids than organophosphates and carbamates.

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Age-related Susceptibility of Spodoptera litura Larvae to Some Insecticides (담배거세미나방 유충의 영기별 약제 감수성)

  • 조점래;송원례;황선영;김홍선;이정운
    • Korean journal of applied entomology
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    • v.35 no.3
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    • pp.249-253
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    • 1996
  • In the laboratory study, the toxicities of chlorpyrifos-methyl, chlorpyrifos, etofenprox, etofenprox+PAF' and deltamethrin for different larval instars of S. litura decreased significantly as larvae aged. LCso values for chlorpyrifos- methyl and chlorpyrifos increased significantly from 3rd instar larvae, while those for etofenprox+PAP and deltamethrin-increased from 2nd instar larvae. In pot study, no significant differences in control efficacy were observed among each treated plots and over 90% control efficacy at 5 d after treatment was obtained in all of the treated plots, except treatment with deltamethrin. Therefore four insecticides excluding deltamethrin will be effective for controlling S. litura. However, it will be probably important to select appropriate insecticides and decide a proper time of treatment because the developmental stage is a significant factor in deciding insecticide efficacy because various developmental stages of the tobacco cutworm inhibit in fields.

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Inheritance of Fenvalerate Resistance in the Diamondback Moth, Plutella xylostella Linne(Lepidoptera: Yponomeutidae) (배추좀나방의 Fenvalerate에 대한 저항성 유전)

  • 김길하;이준호;조광연
    • Korean journal of applied entomology
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    • v.30 no.2
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    • pp.106-110
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    • 1991
  • The mode of inheritance of resistance to fenvalerate in the diamondback moth (Plutella xylostella L.) was studied using insecticide susceptibility and mixed function oxidase tests. Tehre were no differences in the concentration-mortality relationships between $F_1$ progenies $(S_{female}\timesR_{male},\;R_{female}\timesS_{male})$ obtained from reciprocal crosses with the susceptible and fenvalerate-selected strains(R), indicating the absence of sex-linked inheritance. Degree of dominance of the $F_1$ progenies $(S_{female}\timesR_{male},\;R_{female}\timesS_{male})$ were -0.50 and -0.46, respectively, in the insecticide susceptibility test and -0.85 and -0.81, respectively, in the mixed function oxidase test. These results suggest that inheritance of fenvalerate resistance is controlled by and incompletely recessive autosomal gene.

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