• Title, Summary, Keyword: Korean native goat

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Detecting Positive Selection of Korean Native Goat Populations Using Next-Generation Sequencing

  • Lee, Wonseok;Ahn, Sojin;Taye, Mengistie;Sung, Samsun;Lee, Hyun-Jeong;Cho, Seoae;Kim, Heebal
    • Molecules and Cells
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    • v.39 no.12
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    • pp.862-868
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    • 2016
  • Goats (Capra hircus) are one of the oldest species of domesticated animals. Native Korean goats are a particularly interesting group, as they are indigenous to the area and were raised in the Korean peninsula almost 2,000 years ago. Although they have a small body size and produce low volumes of milk and meat, they are quite resistant to lumbar paralysis. Our study aimed to reveal the distinct genetic features and patterns of selection in native Korean goats by comparing the genomes of native Korean goat and crossbred goat populations. We sequenced the whole genome of 15 native Korean goats and 11 crossbred goats using next-generation sequencing (Illumina platform) to compare the genomes of the two populations. We found decreased nucleotide diversity in the native Korean goats compared to the crossbred goats. Genetic structural analysis demonstrated that the native Korean goat and cross-bred goat populations shared a common ancestry, but were clearly distinct. Finally, to reveal the native Korean goat's selective sweep region, selective sweep signals were identified in the native Korean goat genome using cross-population extended haplotype homozygosity (XP-EHH) and a cross-population composite likelihood ratio test (XP-CLR). As a result, we were able to identify candidate genes for recent selection, such as the CCR3 gene, which is related to lumbar paralysis resistance. Combined with future studies and recent goat genome information, this study will contribute to a thorough understanding of the native Korean goat genome.

Identification of Korean Native Goat Meat using DNA Analysis (DNA분석기법을 이용한 한국재래산양육의 판별)

  • Sang, B.C.;Lee, S.H.;Ryoo, S.H.;Seo, K.W.;Han, S.W.;Kim, S.K.
    • Korean Journal of Agricultural Science
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    • v.26 no.2
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    • pp.33-38
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    • 1999
  • This study was carried out to analyze the genetic polymorphisms of genomic DNA of blood and meat for conservation of the genetic resources and genetic improvement of Korean Native goat. The genetic identification between Korean Native goat and imported goat was examined using RAPD(random amplified polymorphisms DNAs) analysis with 30 Korean Native goat, 10 hybrid, 10 imported goat. 10 Korean native goat meat and 10 imported goat meat. The results obtained from this study were summarized as follows: 1. Genomic DNA from Korean native goat, hybrid and imported goat could be obtained above about 23kb size using 0.5% agarose gel electrophoresis and the ratio of optical density at 260nm to that at 280nm was between 1.7 and 2.0 using UV spectrophtometer instrument. 2. In the results of the gene identification between Korean Native goat and hybrid, and imported goat using RAPD methods with random primer of 110 kinds, only Korean native goat showed a specific band at about 369bp using a random primer OPO-19 (5'-CAA ACG TCG G-3'), but imported goat and hybrid not showed. 3. Also, in the results of the gene identification between Korean Native goat meat and imported goat meat using RAPD methods with random primer, Korean native goat only showed a specific band at about 369bp using a random primer No. 19(5'-CAA ACG TCG G-3'), but imported goat not showed.

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The karyotype of Korean native goat (capra hircus) (한국재래산양의 핵형분석)

  • Oh, Seung-hyun;Yun, Young-min;Yoon, Yeo-sung;Lee, Joon-sup;Lee, Heungshik S.;Seong, Je-Kyung
    • Korean Journal of Veterinary Research
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    • v.39 no.5
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    • pp.908-920
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    • 1999
  • We investigated the cytogenetic characteristics of Korean native goat(Capra hircus). Chromosome slides were prepared from peripheral blood cell cultures. GTG, GBG, RBG and CBG-banding techniques were employed on those slides. The high resolution karyotype of Korean native goat could be made with the incorporation of BrdU. Korean native goat has 60 chromosomes composed of 58 autosomes and XY or XX sex chromosomes. All of autosomes of Korean native goat were acrocentric chromosomes. X chromosome was submetacentric and Y chromosome was metacentric. The GTG, GBG and RBG-band patterns of Korean native goat were similar to those of other goats. CBG-band regions were distinct at the proximal portion of the long arms of all autosomes in Korean native goats. According to our investigation, there was no significant difference in chromosomal band patterns between Korean native goat and other goats. It might be necessary to use molecular genetic markers for clarifying the genetical characteristics of Korean native goat whose biological characteristics are not clearly defined.

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An immunohistochemical study of the endocrine cells in the common pancreatic ducts of the Korean native goat (한국재래산양의 대췌관에서 내분비세포의 면역조직화학적 연구)

  • Lee, Jae-hyun;Lee, Hyeung-sik
    • Korean Journal of Veterinary Research
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    • v.37 no.2
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    • pp.263-267
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    • 1997
  • The distribution and relative frequencies of the endocrine cells were studied immunohistochemically in the common pancreatic ducts of the Korean native goat with serotonin, glucagon, insulin, BCG, BPP and somatostatin antisera. Serotonin-, glucagon-, BCG-, BPP- and somatostatin-immunoreactive cells were detected in the basal portion of the mucosal gland in the common pancreatic ducts of the Korean native goat but insulin-immunoreactive cells were not detected. The function of these immunoreactive cells and appearance of other immunoreactive cells in the common pancreatic ducts of the Korean native goat were remains unknown.

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Erythrocyte Sedimentation Rates of Reshuffled Packed Cell Volume in Korean Native Goat (PVC수치와 온도가 한국재래 산양적혈구 침강에 미치는 영향)

  • Lee, Soo Doo;Kim, Young Hong;Yu, Chang Jun
    • Current Research on Agriculture and Life Sciences
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    • v.11
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    • pp.55-59
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    • 1993
  • The packed cell volume(PCV) of Korean native goat, volume percentage of red blood cell in whole blood, was reshuffled of 20%, 40% and 60% using autoplasma, and erythrocyte sedimentation rate was measured in Westergren tubes at room temperature ($27{\pm}1^{\circ}C$) and low temperature ($8{\pm}1^{\circ}C$). The sedimentation rates of red blood cell obtained are summarized as follows. The erythrocyte sedimentation rates of Korean native goat are accelerated more at high temperature than low temperature. The erythrocyte sedimentation rates of reshuffled Korean native goat upon time are almost linear for several hours. The erythrocyte sedimentation rates of Korean native goat are settled faster at low PCV than higher PCV, i. e., there is a reverse relationshif between the erythrocyte sedimentation rate and packed cell volume.

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Studies on The Diseases of the Korean Native Goat-A Review (우리나라 재래염소의 질병에 관한 연구-문헌조사)

  • 이정길;이채용;곽형수
    • Journal of Veterinary Clinics
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    • v.17 no.1
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    • pp.32-44
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    • 2000
  • The Korean native goat has been extensively used in the experimental work. Compared to those experimental work employing the goat, reports on their diseases have been scarcely documented. In the present communication, authors reviewed the literatures pertaining to the diseases of the Korean native goat. Diseases of the respiratory system, the alimentary tract, the liver, the musculoskeletal system, the skin, the urogenital system, the nervous system, the eye, and the heart were reviewed. And then metabolic, toxic, and parasitic diseases were reviewed. Of those diseases reviewed, diseases of the heart and kidney, and metabolic and toxic diseases were wholly artificially-induced. Except the parasitic diseases, a considerable number of diseases of other organ systems were also artificially-induced. And the parasitic diseases reported were confined to the internal parasites. Authors feel that the known occurrence of diseases would help diagnose new disease in the goat.

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Analysis of Genetic Diversity and Relationships of Korean Native Black Goat using Microsatellite Markers (초위성체 마커를 이용한 한국 재래 흑염소의 유전적 다양성 및 유연관계 분석)

  • Park, Byeong Kyu;Kim, Yi Seul;Seong, Jiyeon;Kong, Hong Sik
    • Journal of Animal Reproduction and Biotechnology
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    • v.34 no.3
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    • pp.183-189
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    • 2019
  • The aim of this study was to assess the levels of genetic diversity and relationships of Korean native black goat (n = 58), compared with the exotic breed, Boer (n = 97). For the analysis of genetic characterization 11 microsatellite markers (MAF065, INRA063, CSRD247, OarFCB20, SRCRSP5, INRA006, ILSTS008, ILSTS011, INRA005, ILSTS087, SRCRSP8) were genotyped. The number of alleles was observed 3 (INRA005) to 10 (SRCRSP8) each markers. The mean expected and observed heterozygosity (Hexp and Hobs) and polymorphism information content (PIC) for the Korean native black goat breed varied from 0.551 to 0.860, 0.517 to 0.948 and 0.464 to 0.835, respectively. Principal Components Analysis (PCoA) and FCA results showed that Korean native black goat breed was confirmed to be clearly separated from bore breed. These results were scientific evidence that Korean native black goat represents a unique and valuable animal genetic resource.

Identification of Korean Native Goat Meat using Amplified Fragment Length Polymorphism (AFLP) DNA Markers (Amplified Fragment Length Polymorphism (AFLP) DNA Marker를 이용한 한국 재래흑염소육 감별)

  • 정의룡
    • Food Science of Animal Resources
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    • v.22 no.4
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    • pp.301-309
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    • 2002
  • This study was carried out to develop the breed-specific DNA markers for breed identification of Korean native goat meat using amplified fragment length polymorphism (AFLP)-PCR techniques. The genomic DNAs of Korean native goat, imported black goat and four dairy goat breeds(Saanen, Alpine, Nubian and Toggenburg) were extracted from muscle tissues or blood. Genomic DNA was digested with a particular combination of two restriction enzymes with 4 base(Mse I and Taq I) and 6 base(EcoR I and Hind III) recognition sites, ligated to restriction specific adapters and amplified using the selective primer combinations. In AFLP profiles of polyacrylamide gels, the number of scorable bands produced per primer combination varied from 36 to 74, with an average of 55.5. A total of 555 bands were produced, 149(26.8%) bands of which were polymorphic. Among the ten primer combinations, two bands with 2.01 and 1.26 kb in M13/H13 primer and one band with 1.65 kb in E35/H14 primer were found to be breed-specific AFLP markers in Korean native goat when DNA bands were compared among the goat breeds. In the E35/H14 primer combination, 2.19, 2.03, 0.96 and 0.87 kb bands detected in imported black goat, 2.13 kb band in Saanen breed and 2.08 kb band in Nubian breed were observed as breed-specific bands showing differences between goat breeds, respectively. The E35/H14 primer combination produced four DNA bands distinguished between Korean native goat and Saanen breed. The is study suggested that the breed specific AFLP bands could be used as DNA markers for the identification of Korean native goat meat from imported black goat and dairy goat meats.

Anatomical studies on the ear muscles of the Korean native goat (한국재래산양의 이개근에 대한 해부학적 연구)

  • Lee, Chang-hyun;Lee, Heungshik S.;Lee, In-se
    • Korean Journal of Veterinary Research
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    • v.29 no.3
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    • pp.239-244
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    • 1989
  • This study was carried out to investigate the origin, insertion, direction of muscle fibers and structure of the ear muscles of the Korean native goat. The description was based on the dissection of fifteen the Korean native goats with embalming fluid. The results were as follows: 1. The ear muscles of the Korean native goat were composed of the M. zygomaticoauricularis, M. scutuloauricularis superficialis, M. scutuloauricularis profundus, M. frontoscutularis, M. interscutularis, M. parietoauricularis, M. cervicoscutularis, M. cervicoauricularis superficialis, M. cervicoauricularis medius, M. cervicoauricularis profundus, M. auricularis profundus posterior and M. parotidoauricularis. 2. The M. frontoscutularis clearly seperated into temporal and frontal parts in 6 cases. 3. The M. scutuloauricularis profundus clearly separated into major and minor parts. 4. The M. zygomaticoauricularis blended with the M. parotidoauricularis near its insertion, but not with the M. scutuloauricularis.

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Ontogeny of calcitonin immunoreactive cells in the thyroid glands of Korean native goat (한국재래산양 갑상샘에 있어서 Calcitonin면역 반응세포의 개체발생)

  • Lee, Jae-hyun;Ku, Sae-kwang;Lee, Hyeung-sik
    • Korean Journal of Veterinary Research
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    • v.36 no.3
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    • pp.513-519
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    • 1996
  • The development of calcitonin, serotonin(5-HT) and bovine chromogranin (BCG)-immunoreactive cells was investigated in the thyroid glands of Korean native goat from fetus to adult, using an immunohistochemical method. Moderate number of calcitonin-immunoreactive cells were present in the thyroid glands from 1 month to 6 months after birth, whereas a few in the adult. They were not present in the thyroid glands of fetus and 1 day after birth. A number of 5-HT- and BCG-immunoreactive cells were present in the thyroid glands of Korean native goat from fetus to adult. They were increased in number with age. BCG-immunoreactive cells were weakly reacted in fetus and 1 day after birth, while these cell were strongly stained from 1 month to adult. These findings suggest that calcitonin-, 5-HT- and BCG-immunoreactive cells were present in the thyroid gland of Korean native goat.

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