• Title, Summary, Keyword: LA test

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Serodiagnosis of canine toxoplasmosis by latex agglutination and indirect fluorescent antibody test (Latex 응집반응과 간접형광항체법을 이용한 개 톡소플라즈마병의 혈청학적 진단)

  • Lee, Byung-hoon;Lee, Eung-goo;Suh, Myung-deuk
    • Korean Journal of Veterinary Research
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    • v.32 no.4
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    • pp.641-647
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    • 1992
  • This study was conducted to determine the serum antibodies against toxoplasma in the artificially infected dogs, pet and street dogs by latex agglutination (LA) and indirect fluorescent antibody (IFA) test. LA test was carried out with commercial Toxo-MT kit (Eiken chemical Co.) and IFA test was carried out with rabbit-anti-dog IgG labelled with FITC (Cappel Co.) and toxo-antigen slides prepared in laboratory. The results obtained were as follows ; 1. Antibodies to Toxoplasma gondii in the artificially infected dogs were detected firstly at the Day 8 in IFA and Day 9 in LA test after inoculation. Positive antibody reactions by these tests were declined gradually afterward but maintained up to 12 weeks. 2. In LA test serum antibody titers in 310 test sera were shown as 10 cases(32%) in 1 : 32.5(1.0%) in 1 : 64, 4(1.3%) in 1 : 128 and 2(0.7%) in 1 : 256. In IFA test serum antibody titers 310 test sera were shown as 17 cases(5.5%) in 1 : 64, 8(2.6%) in 1 : 128 and 5(1.6%) in 1 : 256. 3. In the total of 310 sera from pet and street dogs toxoplasma antibody positive rates were 21 cases (6.8%) by LA and 30 cases (9.7%) by IFA test and the positive detection rates between these two groups by LA and IFA test were not significant(p<0.05). 4. In the total of 115 sera from pet dogs toxoplasma antibody positive rates were 12 cases(10.4%) by LA and 15(13.0%) by IFA test. And in the 195 street dogs the positive rates were 9 cases(4.6%) by LA and 15(7.7%) by IFA test. Also, the positive detection rates between these two groups by LA and IFA test were not significant(p<0.05). 5. Agreement of reactivity between LA and IFA test for 310 sera was 91.3% in total of 283 cases consisting of 12 cases(3.9%) of both LA and IFA positive and 271 cases(87.4%) of LA and IFA negative. 6. LA test was almostly equivalent to the IFA test in producibility and proved to be a simple tool for the screening of toxoplasma antibody in laboratory.

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Antimutagenic Effects of Linoleic Acid

  • Lim, Sun-Young;Rhee, Sook-Hee;Park, Kun-Young
    • Preventive Nutrition and Food Science
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    • v.2 no.1
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    • pp.29-34
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    • 1997
  • In order to determine the effectiveness of linoleic acid(LA) to inhibit carcinogens/mutagens-induced mu-tagenesis, Ames test using Salmonella typhimurium TA100 and the SOS chromotest using E. Coli PQ37, were carried out. The inhibitory effect of LA(1%) on the Ames mutagenicity test were 98%, 78%and 69% mediated by aflatoxin B₁(AFB₁), N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) and 4-nitroquinoline-1-oxide(4-NQO), respectively. LA exhibited a strong antimutagenic activity aganist indirect mutagen, AFB₁whereas exhibited the same concentration of LA showed weaker inhibitory effects on direct mutagens of MNNG and 4-NQO than that AFB₁. LA also reduced the SOS responses induced by MNNG and 4-NQO significantly. This result showed a possibility that LA can be a protective agent in early step of cancinogenesis.

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Prevalence of toxoplasma antibody in swine by microplate latex agglutination test in Kyeongnam district (Latex 응집반응에 의한 경남중부지역 돼지의 톡소플라즈마병 항체조사)

  • Lee, Byung-hoon;Kim, Cha-yong;Suh, Myung-deuk
    • Korean Journal of Veterinary Research
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    • v.33 no.2
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    • pp.263-268
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    • 1993
  • This study was conducted to detect the serum antibody of toxoplasma in swine from breeding-pig, rearing-pig farm and slaughtered pig in abattior by latex agglutination(LA) test. The perfomance of LA test was carried out with commercial Toxo-MT kit(Eiken Chemical Co.)by Tsubota and Ozawa's method. The cut-off titer of positive and negative reactions by Toxo-MT antigen used in this experiment was determined as the serum titer of 1 : 32. Positive rate of toxoplasma antibody from the total of 823 serum samples by LA test was 17.0%(140 cases). And positive rates of toxoplasma antibody against serum samples of 194 from breeding-pig farm, 273 from rearing-pig farm and 356 from abattior were 91 cases(46. 9%), 23 cases(8.4%) and 26 cases(7.3%), respectively. The distributions of serum antibody titers in 823 test sera by LA test were shown 51 cases(36.3%) in 1:32, 40(28.6%) in 1:64, 17(12.1%) in 1:128, 14(10.0%) in 1:256, 3(2.1%) in 1:512, 5(3.6%) in 1:1024 and 3(2.1%) in 1:2048. The ranges of positive rate from the sera in each group of breeding-pig farms were 20~61.9%.

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Survey on the Distrributions of Swine Toxoplasma Antibodies by Latex Agglutination Test in Gyeongnam Central Area (경남 중부지역에서의 Latex응집반응을 이용한 돼지 톡스플라즈마병 항체분포 조사)

  • 이병훈;황보훈;변유성;이순선;김차용;서명득
    • Korean Journal of Veterinary Service
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    • v.15 no.2
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    • pp.174-183
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    • 1992
  • This study was conducted to determine the serum antibodies against toxoplasma in swine from breeding-pig farm, pig farm and abattoir by latex agglutination(LA) test. LA test was carried out with commercial Toxo-MT kit (Eiken chemical co.). The results obtained were summerized as follows : 1. The cut-off titer of positive and negative reactions by Toxo-MT antigen used in this experiment was determined as the serum dilution of 1 ; 32. 2. positive rates of toxoplasma antibodies in 823 swine sera were 17.0%(140 cases) by LA test. 3. The toxoplasma antibody detection rates against 194 swine sera in breeding-pig farm, 273 swine sera in pig farm and 356 swine in abattoir were 46.9%(91 cases), 8.4%(23 cases) and 7.3% (26 cases) , respectively. 4. In LA test serum antibody titers in 823 test sera were shown as 51 cases (36.4%) in 1 : 32, 40(28.6%) in 1;64, 17(12.1%) in 1:128, 14(10.0%) in 1:256, 10(7.1%) in 1:512, 5(3.6%) in 1:1,024, and 3(2.1%) in 1 : 2,048. 5. Positive rates of toxoplasma antibodies in swine sera from each breeding-pig farm were 20.0∼61.9%.

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Differentiation of Candida albicans and Candida dubliniensis using Latex Agglutination Test (라텍스 응집 검사를 이용한 Candida albicans와 Candida dubliniensis의 감별)

  • Kim, Tae-Hyoung;Lee, Mi-Kyung
    • Journal of Mycology and Infection
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    • v.22 no.1
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    • pp.15-20
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    • 2017
  • Background: Candida dubliniensis is phenotypically similar to Candida albicans that may be underdiagnosed in clinical laboratory. In 2010, C. dubliniensis was first recovered from blood of a candidemia patient in Seoul, Korea. Also, a simple commercial latex agglutination (LA) test is available. Objective: The aim of the present study was to investigate the prevalence of C. dubliniensis among isolates in our stocks during 2-years period (2010-2011) and to evaluate the ability of LA test (Bichro-Dubli $Fumouze^{(R)}$) for the differentiation of C. albicans and C. dubliniensis. Methods: A total 509 isolates including 504 C. albicans and 5 C. dubliniensis were examined for LA test, the presence of "spiking" on blood agar plate, and the germ tube test. Also all isolates were tested using the VITEK 2 ID-YST system. Results: No C. dubliniensis was found in 504 isolates of initially identified as C. albicans. The LA test was positive only in 5 clinical isolates and 2 type strains of C. dubliniensis. Conclusions: The data show that the prevalence of C. dubliniensis in Korea is still expected to be extremely low and LA test is very rapid, simple, and reliable tool for the differentiation of C. albicans and C. dubliniensis.

Server on the Distribution of Bovine Toxoplasma Antubodies by Latex Agglutination Test in Gyeongnam District (경남지역에서의 Latex응집반응을 이용한 소 톡소플라즈마 항체분포 조사)

  • 이병훈;류중운;황보원;변유성;조광제
    • Korean Journal of Veterinary Service
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    • v.18 no.3
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    • pp.36-41
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    • 1995
  • This study was conducted to determine the serum antibodies against toxoplasma in bovine from gyeongnam district by latex agglutination(LA) test. LA test was carried out with Toxo-MT kit(Eikon chemical co). The result obtained were summerized as follow: 1. Positive rates of toxoplasma antibodies in 1,488 bovine sera was 5.0%(75 cases) by LA test. 2. The toxoplasma antibody detection rates against 823 korean cattle and 865 dairy cattle were 1.8%(11 cases) and 7.4%(64 cases) respectively. 3. In LA test serum antibody titers in 75 positive sera were shown as 54 cases(72.0% in 1:32, 9(12.0%) in 1:64, 7(9.4%) in 1:128, 3(3.40%) in 1:256, 1(1.3%) in 512 and 1(1.3%) in 1:2,048 respectively. 4. Positive rates of toxoplasma antibodies in cattls sera from each area were 0.2∼22.0%.

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A Study on the DC Leakage Current Test for Power Cable of Private Electrical Facilities considering Lightning Arrester (피뢰기를 고려한 자가용 전기설비 인입선로의 직류누설전류시험에 관한 연구)

  • Jeong, Ki-Seok;Gil, Hyoung-Jun
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.67 no.1
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    • pp.142-147
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    • 2018
  • Private electrical facilities are judged whether it is suitable for the insulation aging condition of their incoming underground cables using DC leakage current test method. In the case where the service point of utility is the secondary side of cut out switch installed in the electric pole, there is a problem that it is difficult to separate the lightning arresters(LA) because of their high position of the pole. Therefore, the field test voltage is applied at value lower than DC 30 kV, which are stated in the inspection guideline. However, this test could reduced the insulation performance of the LA by accelerating the electrical stress of the metal oxide varistor element in the pre-breakdown region. In this study, we analyzed the relationship between the DC test voltage and the leakage current using the non-destructive DC high voltage equipment with leakage current measurement function. The results show that the leakage current increases sharply above the specified test voltage. As a consequence, it could be contributed to improve insulation aging inspection method by selecting the possible test area on the VI characteristic curve of the pre-breakdown area of the LA.

Development of diagnostic kit(Test-MT) for the microplate latex agglutination test of toxoplasmosis in animal (Latex 응집반응을 이용한 동물의 톡소플라즈마병 진단용 kit 개발에 관한 연구)

  • Suh, Myung-deuk;Joo, Hoo-don;Maass, Daivd
    • Korean Journal of Veterinary Research
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    • v.35 no.3
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    • pp.583-593
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    • 1995
  • The present study was conducted to develop a toxoplasma latex agglutination test antigen(Test-MT) and evaluate the toxoplasma latex agglutination(LA) test using a newly-made "Test-MT kit" by comparing with the Toxo-MT kit(Eiken chemical co, Tokyo). Also, the specifity and sensitivity test were made by comparing with IFA test and IgG-ELISA. Tachyzoite suspensions of Toxoplasma gondii(RH strain) were ultracentrifuged for 30min at $60,000{\times}g(4^{\circ}C)$ and the supernatant was used as a water-lysate antigen. Polystyrene latex particles of $1.0{\mu}m$ in diameter(Polyscience co) were used for the preparation of sensitized latex-antigen supension(Test-MT). The frequency distribution of LA titers in Test-MT showed two peaks at <1:32 and 1:128. The borderline titer for positive test in Test-MT was determined to be 1:64. But the frequency distribution of LA tites in Toxo-MT showed two peaks at <1:16 and 1:64. The positive borderline was determined to be 1:32. Agreement of reactions between Test-MT and Toxo-MT kit by LA test was shown 92.5% in bovine sera and 97.0% in swine sera, respectively. From the results obtained here it was determined that the sensitized latex-antigen, Test-MT kit, for the microtiter agglutination test prepared as same as by the procedure described in the previous paper(Suh and Lee, 1993) was useful as a highly specific, sensitive and stable immunotiteration reagent for serodiagnosis of toxoplasma infection in animal sera.

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Shangri-La Syndrome of Korean in Their 40s and 50s - Factors Affecting the Syndrome, Conditions, and Tools for Young Appearance - (40대와 50대 성인의 샹그릴라 신드롬에 관한 연구 - 영향 요인, 조건과 표현방법 -)

  • Park, Su-Jin;Yoo, Hwa-Sook
    • Fashion & Textile Research Journal
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    • v.13 no.1
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    • pp.55-65
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    • 2011
  • The purposes of this study were to measure Shangri-La Syndrome through the difference age(i.e. chronological age-cognitive age) of Korean in their 40s and 50s, and to examine the variables which have an effect on the syndrome, requirements of Shangri-La Syndrome and means to look young. The research was conducted on 365 men and women in their 40s and 50s from Ulsan, Busan, and Seoul. The statistical analysis methods used for the study were reliability analysis, factor analysis, frequencies analysis, t-test, correlation analysis, ANOVA, Duncan test, and regression analysis. The results of this study are as follows : First, difference age showed to be 9.5 on the average and appeared to be largest in terms of feel and interests ages and smallest in terms of look and health ages. Second, it was found that Shangri-La Syndrome was influenced by health and self-love factors from among physical and social-psychological characteristics, and age, educational status, job, and hobby out of demographic characteristics. Third, it was shown that health body was the most important condition for a living with Shangri-La Syndrome and clothing was the most effective tool available for young appearances.

Measurement and Evaluation of Optimum Salt Concentration in Usual Dishes for the Menu Suggestion (적염 섭취 식단 모델을 제시하기 위한 상용 음식의 최적 염미도 측정 및 적염 식단 모델)

  • 이혜상;이경신
    • Korean journal of food and cookery science
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    • v.12 no.3
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    • pp.305-311
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    • 1996
  • This study was to seek menu model in usual dishes to guarantee the optimum salt intake considering the optimum gustation of salt surveyed on Korean adults. First, a pilot test was practiced using rice gruel to determine the criteria and a meaningful interval of salt concentration. Based on the criteria and interval so determined, a test was performed to seek the optimum gustation of salt concentration in usual diets. Subjectgroup for the above tests was university students living in Kyung-Book area. The results were as follows: 1. Mode value of the optimum gustation of salt was 0.5% and the received threshold difference of salt was 0.2% in the pilot test. 2. Mode values of the optimum gustation of salt in a la carte, soups and stews were lower than those of other side dishes. 3. But the portion size of a la carte, soups and stews is much larger than that of other side dishes, so that a la carte, soups and stews are sources of relatively larger amount of salt than other side dishes. 4. Considering the recommended optimum salt intake, it is desirable to reduce the amount of stock in soups and stews and to select fried food or salad more frequently.

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