• Title, Summary, Keyword: Lepista nuda

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Genetic Variation Based on Random Amplified Polymorphic DNA (RAPD) and Internal Transcribed Spacer (ITS) Region Sequences in Lepista nuda (RAPD와 ITS 영역에 의한 민자주방망이 버섯의 유전적 변이)

  • Lee, Yang Suk;Kim, Nam Woo;Kim, Jong Bong
    • Journal of Life Science
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    • v.22 no.11
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    • pp.1470-1476
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    • 2012
  • A genetic variation of Lepista nuda and two genus Lepista species (L. irina and L. sordida) were analyzed by random amplified polymorphic DNA (RAPD) and internal transcribed spacer (ITS) sequence analysis. In the resulting RAPD analysis, 22 out of 40 random primers amplified polymorphic RAPD fragment patterns, the amplified bands were 355, and DNA fragment sizes were 200-400bp. Intraspecific genetic dissimilarity of the 10 L. nuda strains were calculated to range from 0% to 21.60%, L. sordida from 16.93% to 24.82%, L. irina were 20.62% to 25.54%, and intraspecific genetic dissimilarity of L. sordida and L. irina was 23.49%. The 673 base pairs were sequenced during the analysis of the ITS I and II region; six L. nuda strains intraspecific genetic dissimilarities ranged from 1.58% to 11.47%, L. nuda and L. sordida from 3.83% to 12.88%, L. nuda and L. irina from 7.11% to 15.61%, and intra-specific genetic variation between L. sordida and L. irina was 4.79%. The findings showed that RAPD and ITS sequencing could be used for developing molecular genetic markers and screening of unidentified genus Lepista species.

Sawdust cultures of Lepista nuda (민자주방망이버섯 (Lepista nuda)의 톱밥배양)

  • Lee, Sang-Sun;Choi, Kyoung-Jin;Oh, Chang-Ho
    • The Korean Journal of Mycology
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    • v.24 no.4
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    • pp.274-279
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    • 1996
  • An isolate of Lepista nuda was cultivated on the solid media consisted of various cereal grains or those of the acacia sawdust mixed with other cereal grains. The mycelia grew well on the solid cultures made from millet cereals, but did not on those from sorghum or corn cereals. Thus, the millet and the acacia sawdust, as the solid media, were employed for the next experiments related to mycelial growths. For growth of this isolate, it was shown that the nitrogen source is needed for those of millet. Also, the rice bran or minerals were considered to be needed for the acacia sawdust. As minerals, however, the rice bran is shown as the best additive in the solid cultures of the acacia sawdust. The mycelia of L. nuda were cultivated directly through the solid cultures. Their growths were measured' by the production of carbon dioxide with gas chromatography.

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Soil Properties of the Habitat of Lepista nuda (민자주방망이버섯 (Lepista nuda) 서식지의 토양인자 분석)

  • Lee, Yang-Suk;Joo, Eun-Young;Kim, Jong-Bong;Kim, Nam-Woo
    • The Korean Journal of Ecology
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    • v.28 no.1
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    • pp.25-29
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    • 2005
  • This study was carried out to analyze the soil properties of Lepista nuda habitats to form a part of the studies on the characteristics of L. nuda. The soil samples collected from seven studied areas were investigated for soil properties such as soil moisture, soil pH, and the contents of organic and inorganic matters. The content of soil moisture was 29.7% and the content of organic matter was 32.1%. Total nitrogen was 0.74% and soil pH was 4.75, which was ranged from 3.6 to 5.20. In the content of inorganic matter, the content of Fe was highest as 1,024 ppm, K 183 ppm, Mn 21.9 ppm, Ca 2.02 ppm, Zn 1.46 ppm and Mg was lowest as 0.51 ppm.

Genetic Variation of Korean Lepista nuda (한국산 민자주방망이 버섯의 유전적 변이)

  • 김승희;김종봉
    • Journal of Life Science
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    • v.14 no.1
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    • pp.115-120
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    • 2004
  • Lepista nuda is a world-wide species which has and international reputation as a excellent edible species. In this study, we investigated the genetic variation and taxonomic relationship of L. nuda and other five Tricholomataceae species were analyzed by random amplied polymorphic DNA (RAPD). 15 kinds of random primers were used. The distance matrix was calculated using UPGMA and phyolgenetic relationship were inferred by neighnor-joining (NJ) method. Various bands of 100bp∼1600bp were observed in electrophoretic patterns of RAPD. Nei's genetic distance was calculated using a total of 228 DNA bands identified, and phylogenetic tree was made. The Nei's genetic variations of L. nuda, Lepista surdida, Collybia peronata, Collybia confluens, Lyophyllum cinerascens, Laccara laccata were 0∼21.3%, 21.2∼28.0%, 15.4∼23.0%, 14∼21.8%, 16.5∼34.6%, and 12.4∼27.4%, respectively The consistency index, the retention index and homoplasy index were 0.5217, 0.5769 and 0.5156, respectively. Also, two groups could be made by NJ tree. The genetic distance between L. nuda and C. confluens was closer than that between L. nuda and L. sordida.

Studies on Inorganic Components of Korean Wild Edible Mushrooms - Trace Mineral Elements of Armillariella Mella, Hygrophorous russula, Armillariella tabescens, Lepista nuda and lepista sordida, Hygrocybe conica. - (한국산 야생(野生) 식용버섯의 무기성분에 관한 연구 - 뽕나무버섯, 뽕나무버섯부치, 벚꽃버섯, 민자주방망이버섯, 자주방망이버섯아재비, 붉은산무명버섯의 미량금속원소 -)

  • Park, Wan-Hee
    • The Korean Journal of Mycology
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    • v.21 no.4
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    • pp.273-278
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    • 1993
  • In order to determine of trace mineral elements of wild edible mushrooms in Korea, the dried carpophores of Armillariella mella(Vahl.ex Fr.) karst., Armillariella tabescens(Scop.) Sing., Lepista nuda(Bull.ex Fr.) Cooke and Lepista sordida(Schum.ex Fr.) Sing., Hygrophorus russula (Schaeff.ex Fr.) Qu'el., Hygrocybe conica(Scop.ex Fr.) Kummur were incinerated and analyzed by an atom adsorption spectrophotometry. The six mushrooms contained ubiquitously potassium, iron, zinc, sodium, manganase, copper and calsium in that order, and the content of potassium was the highest than other inorganic components and the content of zinc in Hygrocybe conica was especially the most than the other five mushrooms. Total contents of inorganic components(except Cd) were universally the highest in Hygrocybe conica than other five mushrooms, and decrease in order Hygrophorus russula, lepista nuda, lepista sordida, Aarmillariella mella and Armillariella tabescens. Calsium was not present in Amillariella mella and lepista nuda, and copper not in Hygro­cybe conica, and content of cadimium in Hygrophorus russula and Hygrocybe conica were trace.

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Characterization of a Fibrinolytic Serine Protease from a Wild Mushroom, Lepista nuda

  • Kim Jun-Ho
    • Biomedical Science Letters
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    • v.12 no.3
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    • pp.225-231
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    • 2006
  • Fibrinolytic enzyme was purified from the fruiting bodies of Lepista nuda, using DEAE-Cellulose chromatography, Phenyl Sepharose chromatography, and Mono-S column chromatography. The substance has a molecular weight of 30006.62 Da as measured by MALD-TOF mass spectrometry. The N-terminal amino acid sequence of the enzyme was Tyr-Pro-Ser-Pro-Ser-His-Gln-Thr-Ala-Val-Asn-Ala-Ile-Ile-X. The activity of the enzyme was inhibited by PMSF, indicating that the enzyme is a serine protease. No inhibition was found with E-64, pepstatin, and EDTA. It has broad substrate specificity for synthetic peptides. The enzyme was stable up to $30^{\circ}C$. The enzyme hydrolyzes both Aa and y chains of human fibrinogen but did not show any reactivity for $B{\beta}$ chain of human fibrinogen.

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A study of morphological characteristics and hybridization on Lepista nuda (민자주방망이버섯(Lepista nuda )의 형태적 특성 및 교배에 관한 연구)

  • Jung, Won Soon;Kim, Jong Bong
    • Journal of Mushroom
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    • v.11 no.1
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    • pp.1-8
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    • 2013
  • In this study, twelve of Lepista nuda were collected from various localities in Korea. Also thirteen exotic L. nuda species were collected from Japan, France, Switzerland and Portugal. Spores were isolated under optical microscope. These spores were placed on the surface of YM medium for inducing to germination. Eleven mating-groups were selected by morphological characters of fruit body such as size, color and stipe patterns. Intra-isolate crosses were made between two single-spore isolates derived from mating-groups. Also, dikaryotic crossing using the isolates from L. nuda were carried out to evaluated tetrakaryon formation. Cross-mating compatibility tests also verified its dikaryotic state by microscopic or molecular genetic observation of clamp connection and Random Amplified Polymorphic DNA (RAPD) band pattern. To analyze the growth rate of hybrids and parents mycelium in dikaryons obtained from compatible mating groups were placed on PDA medium. Intra-isolate crosses determined eleven mating-groups within L. nuda. The typical clamp connection were mostly observed in mating-groups of Korean L. nuda in $K1{\times}K2$, $K1{\times}K3$, $K1{\times}K4$, $K1{\times}K6$, $K1{\times}K5$, $K2{\times}K4$, $K2{\times}K3$, $K2{\times}K6$, $K3{\times}K4$, $K4{\times}K5$, and $K4{\times}K6$. Korean L. nuda type of dikaryon, shown to cross-incompatibility with L. sordida, it seemed that mating induce more rapidly than wild types in a view of growth rate. In conclusion, it would be useful to improve mass production with better morphological characteristics through a special mating of L. nuda.

Mycelial Culture Conditions of Lepista nuda and Extracellular Enzyme Activity (민자주방망이버섯(Lepista nuda) 균사체 배양조건 및 효소활성)

  • Kim Sang-Dae;Kim Ji-Hye;Kim Jong-Bong;Han Yeong-Hwang
    • Korean Journal of Microbiology
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    • v.41 no.3
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    • pp.164-167
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    • 2005
  • The culture condition and medium composition for the enhanced mycelial growth of Lepista nuda DGUM 26501 were investigated. The optimal temperature and pH for the mycelial growth were $24^{\circ}C$ and $7.0\~8.0$, respectively. The partial pressure of oxygen for the enhanced mycelial growth was more than $10\%\;O_2$. When Czapek-Dox medium was used as a minimal medium, manitol and xylitol were very good carbon sources. Organic nitrogen sources were better than inorganic ones for mycelial growth. As the nitrogen source tested, com steep liquor, soytone and protease peptone were the best as a source of organic nitrogen sources. When ammonium phosphate as phosphorus sources was used, the enhanced mycelial growth was shown. Nicotinic acid was proved to be the most appropriate source of vitamin. After the mycelia of L. nuda DGUM 26501 was cultivated at $24^{\circ}C$ for 10 days in LNM broth (pH 7.0), the activities of extracellular enzyme were determined. The specific activity of $\alpha-amylase$ was much higher than those of other enzymes. However, little or no enzyme activities of $\beta-glucosidase$, CMCase, laccase and lipase were found.

Study on the Antioxidant Activity of the Extracts from the Lepista nuda (민자주방망이버섯(Lepista nuda)의 항산화성에 관한 연구)

  • Lee, Yang-Suk;Park, Dong-Cheol;Joo, Eun-Yong;Shin, Seung-Ryeul;Kim, Nam-Woo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.7
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    • pp.942-947
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    • 2005
  • This study was investigated to analyze the effect of extracts from the Lepista nuda, on the antioxidant activity to form a part of studies on the functional materials of L. nuda. Antioxidant activity of L. nuda extracts was evaluated by measuring the electron-donating ability (EDA), the superoxide dismutase (SOD)-like activity, and the nitrite-scavenging ability. The EDAs of water and ethanol extracts at the concentration of 1,000 ppm by the rotary heating method from L. nuda were $60.47\%$ and $60.13\%$, respectively and those of water and ethanol extracts by microwave-assisted method were $87.73\%$ and $84.84\%$, respectively. The measurements of SOD-like activity were in the range of $24.58\%\~42.03\%$ at 1,000 ppm. EDA and SOD were increased with the concentrations of extracts. The nitrite-scavenging ability at the concentration of 1,000 ppm was the highest and $29.77\%$ at pH 1.2, and was decreased with an increment of pH value. These results indicated that microwave­assisted water extract from L. nuda showed the highest activities on the EDA and nitrate-scavenging ability, while the rotary heating ethanol extract had the highest effect on the SOD-like activity.

Purification and Characterization of Fibrinolytic Enzyme from Lepista nuda (민자주방망이버섯으로부터 혈전용해효소의 정제 및 특성 연구)

  • Kim, Jun-Ho
    • The Korean Journal of Mycology
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    • v.33 no.2
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    • pp.69-74
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    • 2005
  • Fibrinolytic enzyme has been isolated and purified from the edible mushroom, Lepista nuda. The apparent molecular mass of purified enzyme was estimated to be 34 KDa by SDS-polyacrylamide gel electrophoresis. The N-terminal amino acid sequence of the enzyme was Tyr-Pro-Ser-Pro-Ser-His-Gln-Thr-Ala-Val-Asn-Ala-Ile-Ile-X. It has a pH optimum at $7.0.{\sim}9.5$, suggesting that the purified enzyme is an alkaline protease. It shows the maximum fibrinolytic activity at $55^{\circ}C$. The fibrinolytic activity was inhibited by phenylmethylsulfonyl fluoride, indicating that the purified enzyme is a serine protease. The activity of the purified enzyme was totally inhibited by $Hg^{2+}$.