• Title, Summary, Keyword: Linoleic Acid

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Biohydrogenation of Linoleic Acid and Stearic Acid Production by Mixed Rumen Fungi and Bacteria (반추위내 서식하는 혼합곰팡이와 박테리아에 의한 Linoleic Acid 가수소화반응과 Stearic Acid 생산에 관한 연구)

  • Nam, In-Sik
    • Korean Journal of Microbiology
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    • v.43 no.2
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    • pp.100-105
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    • 2007
  • The objective of this study was to confirm biohydrogenation of linoleic acid and stearic acid production by mixed men fungi and bacteria. In mixed fungal biohydrogenation study, when linoleic acid solution was added to fungal culture (after 24 hr pre-incubation), all linoleic acids were converted to trans-11 vaccenic acid via cis-9, trans-11 conjugated linoleic acid production within 24 hr period of incubation. All linoleic acid solution was hydrogenated to trans-11 vaccenic acid within 24 hr incubation and this was continued until the end of incubation (48 hr). Both treatments (added linoleic acid solution or the same amount of solution without containing linoleic acid into fungal cultures) produced the similar amount of stearic acid. In contrast, 100% of linoleic acid solution was hydrogenated to stearic acid in mixed bacterial culture. It is concluded that the end product of mixed fungal biohydrogenation of linoleic acid is trans-11 vaccenic acid whereas mixed bacteria produced stearic acid as an end product of their biohydrogenation.

Isolation and Identification of Rumen Fungus and Its Produced Conjugated Linoleic Acid (반추위에서 분리한 곰팡이의 Conjugated Linoleic Acid 생산과 ITS-1 영역의 염기서열 해석)

  • Nam, In-Sik
    • Korean Journal of Microbiology
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    • v.43 no.2
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    • pp.111-115
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    • 2007
  • The aim of this study was to isolate and identify men fungus which produces conjugated linoleic acid. IS-13 fungus hydrogenated conjugated linoleic acid and trans-11 vaccenic acid within 12 hr after addition of linoleic acid. The homology of IS-13 rumen fungus was compared with internal transcribed spacer 1 region (ITS1)sequences of twenty three men fungi. The length of ITS1 region of IS-13 isolate was 218 bp. IS-13 isolate has the most similar sequence (98% matched) with Orpinomyces species according to maximum-likehood and distance matrix results. The result supported that IS-13 isolate belonged to Orpinomyces genus.

A Study on The Changes of Linoleic Acid Concentration in Major Domestic Forage Species (국내 주요 조사료의 Linoleic acid 농도변화에 관한 연구)

  • Park, Hyung Soo;Seo, Sung;Lim, Young Chul;Choi, Ki Chun;Kim, Ji Hea;Lee, Ki Won;Kim, Jong Geun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.33 no.4
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    • pp.263-268
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    • 2013
  • We investigated the range of linoleic acid concentrations in different forage species and harvest stages. The linoleic acid concentrations in main cultivated grasses and forage crops were analyzed at three harvesting dates in Korea. The experiment compared 19 species of main grasses and forage crops, including eight species of grasses (Perennial ryegrass, Reed canarygrass, Tall fescue, Timothy, Bromegrass, Kentucky bluegrass, Orchardgrass and Wheat grass), six legumes (White clover, Red clover, Sweet clover, Crimson clover, Alfalfa and Hairy vetch) and five forage crops (Italian ryegrass, Barley, Rye, Oat and Rape) in Korea with three cuts (8 May, 19 May and 28 May). The linoleic acid concentrations of Reed canarygrass and Timothy were the highest, and Bromegrass was the lowest among the grass species. All grass species had high concentrations of linoleic acid at the late May harvest stage but were low at the mid May harvest stage. Legumes had higher linoleic acid concentrations than those of grasses, and harvesting in mid-May resulted in the highest linoleic acid concentration. Rape had the highest linoleic acid concentration and rye showed high concentrations of linoleic acid when compared with those of forage crops. All species of grasses and forage had decreased linoleic acid concentrations by the harvest stage. We have demonstrated opportunities to change the composition of ruminant products through breeding, selection, and management of grasses for altered levels of linoleic acid as a precursor to conjugated linoleic acid.

Composition of Fatty Acid in the Edible Oils (시판(市販) 식용유(食用油)의 지방산조성(脂肪酸組成)에 관關하여 (제 1보)(第 1報))

  • Lee, S.J.;Lee, M.J.
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.6 no.1
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    • pp.49-53
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    • 1977
  • Fatty acid composition of commercial oil were analyzed with gas liquid chromatography. Sesame, perilla, rice bran, sunflower, and soy-bean oil were obtained from the whole sale store of edible oil in market. The fatty acids were methylated with Na-methylate. The fatty acid methylester was charged to the gas liquid chromatography. Sesame were composed of myristic, palmitic, stearic. linoleic acid, and trace of linolenic acid. Rice bran, and soy-bean oil were composed of myristic, stearic, oleic, linoleic, and linolenic acid. Peilla oil was composed of palmitic, stearic, oleic, linoleic, and linolenic acid. Sunflower oil was composed of palmitic, stearic, oleic, and linoleic acid.

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Characteristics of Linoleic Acid Production by Marine Fungi in Sea Water Media (해수배지를 이용한 해양 미생물의 Linoleic acid 생성 특성 규명)

  • 김수정;박경원;허병기
    • KSBB Journal
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    • v.15 no.2
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    • pp.195-200
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    • 2000
  • Studies were made on the optimization of media to c비tivate Thraustochytrium aureum A TCC 34304 for the enhanc엉d p production of linoleic acid. The medium optimization was made with the artificial sea water medium. Yeast extract, sodium g glutamate, peptone and tryptone were considered as nitrogen source. The effect of $\infty$ncentration of nitrogen source as well a as initial glucose on the production of linoleic acid were investigated to optimize the media. The maximum yield of lipid was 0 0.302 mg/g cell mass when initial glucose $\infty$ncentration was 10 g/L and sodium glutamate was used as nitrogen source, a and the yield of linoleic acid to unit cell mass was also maximum to be 8 % in that case. The highest linoleic acid c concentration was obtained in the initial glucose concentration 30 g/L regardless of the kinds of nitrogen source and the | linoleic acid concentration was 0.208 g/L when peptone was supplemented to be 2 g/L.

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Oxidation Characteristics of Biodiesel and Its Blend Fuel I (바이오디젤 및 바이오디젤 혼합 연료의 산화 특성 I)

  • Jung, Chung-Sub;Dong, Jong-In
    • Applied Chemistry for Engineering
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    • v.18 no.3
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    • pp.284-290
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    • 2007
  • Biodiesel and its blend fuels from soybean oil were characterized for their oxidation to apply automobile fuel from the analysis of FAME (fatty acid methyl ester) and chemical properties. Biodiesel produced from soybean oil contained unsaturated fatty acids (> 85 wt%) such as oleic acid, linoleic acid, and linolenic acid. Especially, polyunsaturated fatty acids such as linoleic acid and linolenic acid containing active methyl radical were over 60 wt%. It is believed that linoleic acid and linolenic acid cause oxidation. Linoleic acid and linolenic acid during oxidation were major reactants, and compounds with the carbon number having around 36 (boiling point of about $500^{\circ}C$) were produced from those of radical autoxidation.

Synthesis of Glucuronic Acid Conjugates of Linoleic Acid Metabolites (리놀레산 대사체들의 글루쿠론산 결합화합물 합성)

  • Kang, Dong Wook
    • Journal of the Korean Chemical Society
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    • v.57 no.6
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    • pp.738-743
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    • 2013
  • Linoleic acid and its metabolites have various medicinal effects with carboxylic acid functional group. General carboxylic acid compounds are discovered as glucuronide metabolites by UGT glucuronosyl transferase at liver. Consequently, glucuronides of linoleic acid metabolites are expected as potent conjugated metabolite. A previous study reported two epoxide metabolites and two dihydroxy metabolites of linoleic acid. There are prepared their glucuronic acid conjugated compounds as potent linoleic acid metabolites.

Effects of C18 Fatty Acids on Intracellular $Ca^{2+}$ Mobilization and Histamine Release in RBL-2H3 Cells

  • Kim, Myung Chul;Kim, Min Gyu;Jo, Young Soo;Song, Ho Sun;Eom, Tae In;Sim, Sang Soo
    • The Korean Journal of Physiology and Pharmacology
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    • v.18 no.3
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    • pp.241-247
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    • 2014
  • To investigate the underlying mechanisms of C18 fatty acids (stearic acid, oleic acid, linoleic acid and ${\alpha}$-linolenic acid) on mast cells, we measured the effect of C18 fatty acids on intracellular $Ca^{2+}$ mobilization and histamine release in RBL-2H3 mast cells. Stearic acid rapidly increased initial peak of intracellular $Ca^{2+}$ mobilization, whereas linoleic acid and ${\alpha}$-linolenic acid gradually increased this mobilization. In the absence of extracellular $Ca^{2+}$, stearic acid ($100{\mu}M$) did not cause any increase of intracellular $Ca^{2+}$ mobilization. Both linoleic acid and ${\alpha}$-linolenic acid increased intracellular $Ca^{2+}$ mobilization, but the increase was smaller than that in the presence of extracellular $Ca^{2+}$. These results suggest that C18 fatty acid-induced intracellular $Ca^{2+}$ mobilization is mainly dependent on extracellular $Ca^{2+}$ influx. Verapamil dose-dependently inhibited stearic acid-induced intracellular $Ca^{2+}$ mobilization, but did not affect both linoleic acid- and ${\alpha}$-linolenic acid-induced intracellular $Ca^{2+}$ mobilization. These data suggest that the underlying mechanism of stearic acid, linoleic acid and ${\alpha}$-linolenic acid on intracellular $Ca^{2+}$ mobilization may differ. Linoleic acid and ${\alpha}$-linolenic acid significantly increased histamine release. Linoleic acid (C18:2: ${\omega}$-6)-induced intracellular $Ca^{2+}$ mobilization and histamine release were more prominent than ${\alpha}$-linolenic acid (C18:3: ${\omega}$-3). These data support the view that the intake of more ${\alpha}$-linolenic acid than linoleic acid is useful in preventing inflammation.

Studies on Screening and Isolation of Esterase Inhibitors from Soil Microorganisms (II). Isolation of Inhibitors and Associated Lipids from Streptomyces Strain DMC-498 (한국 토양균중 Esterase 저해제 검색 및 분리에 관한 연구(제 2 보) Streptomyces Strain DMC-498 균주의 저해성분 및 관련 지질의 분리)

  • 이승정;김하원;곽진환;심미자;허영근;김성원;최응칠;김병각
    • Journal of Food Hygiene and Safety
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    • v.2 no.4
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    • pp.181-189
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    • 1987
  • To find esterase inhibitors and in the metabolites of Streptomyces strain DMC-498, two active compounds were isolated from the methanol extract of the mycelia of the strain by Silica gel column chromatography and preparatory argentation TLC. These compounds were proved to show competitive inhibition. Compound B was found to consist of linoleic and oleic acids. Fifty percent inhibition concentration ($lC_{50}$) of linoleic acid was $0.045\mu\textrm{g}/ml$, whereas oleic acid exhibited no inhibitory activity. Associated lipids: isostearic acid, isostearic acid methyl ester, oleic acid methyl ester and linoleic acid methyl ester, were isolated from the same extract, showing no inhibition of the esterase. Compound A was found to be a liquid inhibitor with an alicyclic ring and two or more oxygens, its molecular weight being more than 500.

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Effects of Essential Fatty Acids during In Vitro Maturation of Porcine Oocytes: Hormone Synthesis and Embryonic Developmental Potential

  • Kim, Kang-Sig;Park, Hum-Dai
    • Journal of Animal Reproduction and Biotechnology
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    • v.34 no.2
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    • pp.75-85
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    • 2019
  • Omega-3 α-linolenic acid and omega-6 linoleic acid are essential fatty acids for health maintenance of human and animals because they are not synthesized in vivo. The purpose of this study was to evaluate the effect of α-linolenic acid and linoleic acid supplementation on in vitro maturation and developmental potential of porcine oocytes. Various concentrations of α-linolenic acid and linoleic acid were added into in vitro maturation medium, and we evaluated the degree of cumulus expansion, oocyte nuclear-maturation rate, blastocyst rate, blastocyst quality, and levels of prostaglandin E2, 17β-estradiol, and progesterone in the spent medium. High doses (100 μM) of α-linolenic acid and linoleic acid supplementation significantly inhibited cumulus expansion and oocyte nuclear maturation, and prostaglandin E2 synthesis also significantly decreased compared with other groups (p < 0.05). Supplementation of 50 μM α-linolenic acid and 10 μM linoleic acid showed higher quality blastocysts in terms of high cell numbers and low apoptosis when compared with other groups (p < 0.05), and synthesis ratio of 17β-estradiol / progesterone also significantly increased compared with control group (3.59 ± 0.22 vs. 2.97 ± 0.22, 3.4 ± 0.28 vs. 2.81 ± 0.19, respectively; p < 0.05). Our results indicated that supplementation with appropriate levels of α-linolenic acid and linoleic acid beneficially affects the change of hormone synthesis (in particular, an appropriate increase in the 17β-estradiol / progesterone synthesis ratio) for controlling oocyte maturation, leading to improved embryo quality. However, high doses of α-linolenic acid and linoleic acid treatment results in detrimental effects.