• Title/Summary/Keyword: M segment

Search Result 17, Processing Time 0.179 seconds

Molecular Characterization of Hantavirus Isolates from Bandicota indica Captured in Indonesia and Thailand (인도네시아와 태국의 Bandicota indica 폐장조직에서 분리된 한타바이러스의 분자생물학적 특징)

  • Chu, Yong-Kyu;Cui, Longzhu;Song, Dae-Yong;Woo, Young-Dae;Praseno, Praseno;Leitmeyer, Katrin;Lee, Ho-Wang
    • The Journal of Korean Society of Virology
    • /
    • v.30 no.3
    • /
    • pp.203-210
    • /
    • 2000
  • Hantaviruses are etiologic agents of hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS) in the world. Various hantaviruses were isolated from HFRS patients and several different rodent species in the world. Four hantavirus isolates from Indonesia and three isolates from Thailand among 89 Bandicotas captured in Yogyakarta, east region of Sumatra island, Indonesia and at Chiang Mai in Thailand during 1996 were made through several passages in Vero E6 cells. Viral genome M segment from two Indonesian isolates and three Thailand isolates were amplified using hantavirus generic primers of the M segment and cloned into pCRII vector. The genetic differences were analyzed by comparison of partial sequence of the M segment and antigenic differences were made by IFA. Nucleotide sequence homology of two isolates BC 8, BC 34 from Indonesia and two isolates thai 1322, thai 1330 to Seoul virus was 99% and 96%, respectively, but Thai 1164 was 80%Thai 1164 strain has shown 95% homology to Thai 749 virus. In conclusion it is indicated that two different serotype hantaviruses, Seoul and Thailand, are cocirculating among Bandicota in Thailand, in contrast Seoul serotype virus is circulating in Indonesia.

  • PDF

Molecular Characterization of a New Hantaan Virus Howang Strain (한탄바이러스 호왕균주의 M, S 유전자 절편의 염기서열 및 분자생물학적 특성)

  • Chu, Yong-Kyu;Lee, Ho-Wang
    • The Journal of Korean Society of Virology
    • /
    • v.27 no.1
    • /
    • pp.59-68
    • /
    • 1997
  • Hantaan virus Howang strain which isolated from the blood of severe case of Korean hemorrhagic fever is more virulent than HTN 76/118 and showed different RFLP from partial PCR amplifed M genome segment to established Hantaan serotype viruses. We have determined the nucleotide sequence of the M and S genome segments and compared to HTN 76/118. The M and S segment of Howang strain has 3615 and 1696 nucleotides long, respectively. The M segment sequence of Howang strain is one mucleotide shorter than HTN 76/118. The sequence data of Howang strain shows 93.5% homology to HTN 76/118. One long open reading frame, which strats from 41nt. to 3448nt. of the M segment and from 37nt. to 1326nt. of the S segment, exist to on complementary sense of the virus genome. There are no significant difference between HTN 76/118 and Howang strain on hydrophobicity of deduced polypeptides, but has slight difference on secondary structure.

  • PDF

Correlation of Virologic Property and Phylogenetic Analysis of Hantaan Viruses Isolated from Patients and Reservoirs in Korea

  • Jung, Yong-Tae;Lee, Sun-Ryang;Paik, Soon-Young;Kim, Gum-Ryong
    • The Journal of Korean Society of Virology
    • /
    • v.28 no.1
    • /
    • pp.11-20
    • /
    • 1998
  • Twenty isolates of Hantavirus were isolated from patients and reserovirs from 1988 to 1994 in Korea. Isolation rate was 1.9% (10/538) in patients, 6.2% (5/81) in Apodemus sp., 2.6% (1/38) in Rattus sp. and 0.6% (4/677) in bats. Reciprocal mean IFA titers ranged from 27.5 to 1,024 at the specimen collection. According to the growth rate and reaching peak titier of infectivity, the isolates were grouped as rapid, intermediate, and slow growing groups. All isolates were confirmed as Hantaan type by the nested RT-PCR on the G1 region of the M segment. Comparison of nucleotide sequence (Nt: 2101 - Nt: 2280) of the G2 region revealed that the sequence homology bewteen Hantaan 76/118 virus and the isolates was more than 90%. Several nucleotide positions of the isolates showed high variation. The variation rate of patientisolates was about one-half when compared with that of rodentisolates. On the basis of phylogenetic analysis Hantaan viruses isolated were divided into two genogroups. These results indicate that Hantaan virus is highly dominant serotype in Korea and the virologic property and genogroup are not correlated.

  • PDF

Partial Sequence Analysis of Puumala Virus M Segment from Bats in Korea

  • Yun, Bo-Kyoung;Yoon, Jeong-Joong;Lee, Yun-Tai
    • The Journal of Korean Society of Virology
    • /
    • v.29 no.1
    • /
    • pp.23-31
    • /
    • 1999
  • Hantavirus is a genus of the Bunyaviridae family causing two serious diseases, hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). Puumala virus is a member of hantavirus originally found in Europe, and its natural reservoir is Clethrionomys glareolus. It is also associated with the human disease nephropathia epidemica, a milder form of HFRS. To identify the hantaviruses in bats, bats were collected from Jeong-Sun, Won-Joo, Chung-Ju and Hwa-Cheon area in Korea, and nested RT-PCR was performed with serotype specific primer from M segment. Interestingly, Puumala virus was detected in bats (Rhinolophus ferrum-equinum) only from Won-Joo. The 327 bp nested RT-PCR product, was sequenced. The sequence database search indicates that the sequence is homologous to the published sequence of Puumala viruses. The sequence similarities were ranged from 71% to 97%. The highest sequence similarity was 97% with Puumala virus Vranicam strain, and the lowest was 71% with Puumala virus K27 isolate. Puumala virus Vranicam strain was isolated from a bank vole (Clethrionomys glareolus) in Bosnia-Hercegovina. Puumala virus K27 was isolated from human in Russia. This analysis confirms that bats (Rhinolophus ferrum-equinum) in Korea are natural reservoir of Puumala virus.

  • PDF

A Study on Nonlinear Behavior Characteristics of Precast Segmental Gider (프리캐스트 세그먼트 거더의 비선형거동 특성에 관한 연구)

  • Hong Sung Nam;Koh Byung Soon;Kim Kwang Soo;Park Sun Kyu
    • Proceedings of the Korea Concrete Institute Conference
    • /
    • /
    • pp.89-92
    • /
    • 2004
  • Precast Segmental method was developed in germany 1950's. This method has been adoptted in long span girder mainly owing to easy construction effect. But, so far, The limit exists that this method is constructed in a portion of span and hard conveyance and foundation. This study was performed to analysis behavior difference of two rectangular section girder, spliced girder that was jointed 5-sliced 0.8m segment and monolithic girder that was produced in one body 4m span.

  • PDF

An Experimental Study for Mechanical Behavior of Multi-Segment Girder (분리형 거더의 역학적 거동 특성에 관한 실험)

  • 서봉원;김광수;박선규;김수만;이종은
    • Proceedings of the Korea Concrete Institute Conference
    • /
    • /
    • pp.186-189
    • /
    • 2003
  • Precast Segmental method was developed in germany 1950s. This method has been adoptted in long span girder mainly owing to easy construction effect. But, so far, The limit exists that this method is constructed in a portion of span and hard conveyance and foundation. This study was performed to analysis behavior difference of two rectanglar section girder, spliced girder that was jointed 5-sliced 0.8m segment and monolithic girder that was produced in one body 4m span

  • PDF

Dynamic Performance Evaluation of Concrete Barrier Systems for Small Vehicle Roadway (소형차 전용도로를 위한 콘크리트 차량방호울타리의 동적성능 평가)

  • Hwang, In-Tae;Lee, Dong-Woo;Choi, Hyun-Ho;Woo, Kwang-Sung
    • International Journal of Highway Engineering
    • /
    • v.14 no.4
    • /
    • pp.113-124
    • /
    • 2012
  • PURPOSES : In This study two different concrete barrier systems have been proposed to be established at the small vehicle driveway. One is for median barrier, and the other is for roadside barrier. METHODS : In order to determine the suitable shape of barrier, the impact parameters including vehicle weight, impact angle, impact velocity and impact level have been analyzed. The real crash test has been carried out with 0.9 ton and 2.5 ton vehicles, respectively by using the 2m segment type concrete barriers connected by steel plates that are totally 40m barrier systems. RESULTS : The numerical results obtained by LS/DYNA-3D software are compared with real crash tests from the viewpoints of vehicle stability, vehicle trajectory, occupant risk, etc. CONCLUSIONS : From the above results, the dynamic performance of proposed barrier systems satisfies the specification of Korean Code for roadside safety structures.

Distribution of Biomass and Production in Man-made Pitch Pine Plantation in Korea (리기다 소나무 인공조림지의 물질생산량에 관한 연구)

  • Yim Kyong-bin;Lee Kyong-jae;Kwon Tae-ho;Park In-hyeop
    • Journal of Korea Foresty Energy
    • /
    • v.2 no.2
    • /
    • pp.1-12
    • /
    • 1982
  • To study tile comparison of aboveground biomass of Pinus rigida Mill. of 18-year-old, plantations located in Whaseong, Yuseong and Wanju district were selected. Ten sample trees in each district selected taking account of DBH distribution were felled carefully to minimize loss of branches and stem analysed by 1m lag segment sectioned from base . The tree height and DBH were measured for sample trees in total growing within $200m^2$ experimental plot. The diagram of oven-dry weight distribution of stem, branch and needle for each 1m segment was constructed. The logarithmic regression equations between dry weight of each component and the two variables, $DBH^2$ and tree height, combined term were presented. The standing crops in the sample stand was estimated to be as much as 23.88, 54.09 and 42.68 tons of dry matter, above ground , per ha in Whaseong, Yuseong anf Wanju district respectively. Annual net production was estimated at 253,657 and 3.65 tons per ha per year respectively. The net assimilation rate was 1.65,1.95 and 1.81 kg/kg/yr in Whaseong, Yuseong and Wanju district respectively. The efficency of leaf to produce stem was 0.99, 1.12 and 1.30 kg/kg/yr respectively.

  • PDF

Detection of Puumala and Hantaan Viruses among Bats in Korea by Nested RT-PCR

  • Lee, Yun-Tai;Yun, Bo-Kyoung;Yoon, Jeong-Joong
    • The Journal of Korean Society of Virology
    • /
    • v.28 no.2
    • /
    • pp.147-155
    • /
    • 1998
  • Hantavirus is a genus of the Bunyaviridae family consisting following serotype groups: Hantaan, Seoul, Puumala, Prospect Hill, Thailand, Belgrade, Thotta palayam, Sin Nombre. Most of Hantavirus group have been associated with many clinically similar disease known collectively as hemorrhagic fever with renal syndrome (HFRS). Hantaan virus is the prototype of the genus hantavirus, originally isolated from Apodemus agrarius. Bat was found as a natural host for Hantaan virus in Lee's lab for the first time. Then, Hantaan-like virus was isolated Hantaan-like virus from bat. To identify hantaviruses that are present in Korea among bats, bats were collected from Jeong-Sun, Won-Joo, Chung-Ju and Hwa-Cheon area, RNA was isolated from lung and serum. RT-PCR was performed with a universal primer from M segment. Nested RT-PCR was carried out to differentiate Hantaan, Seoul and Puumala virus using serotype specific primers. As we expected, Hantaan viruses were detected in bats and Seoul virus was not detected. Interestingly, Puumala viruses were also detected in bats from Won-Ju, but not in other areas. Puumala virus is originally isolated from Clethrinomys glareolus, and cause light HFRS. Recently, Paradoxomis webbiana, a wild bird turn out to be a reservoir for Puumala virus in Korea. These data indicate that bat is a new natural reservoir of Puumala virus.

  • PDF

Flexural Behavior of Precast PSC Segmental I Girder (Precast PSC-Segmental I형 거더의 휨거동에 관한 연구)

  • Hong, Sung Nam;Kim, Kwang Soo;Park, Sun Kyu
    • KSCE Journal of Civil and Environmental Engineering Research
    • /
    • v.26 no.3A
    • /
    • pp.421-428
    • /
    • 2006
  • This study was performed by using experiment to minimize behavior difference of Monolithic and segmental Girder and to prove the design concept of the PSI (Precast PSC-Segmental I Grider). A full scale girder test was performed in four different cases, the monolithic girder, the segmental girder type-1, the segmental girder type-2 and the segmental girder type-3. The monolithic girder that was produced in one body 25 m span and the segmental girder that was jointed 5-sliced 5 m segment. The girder was built by as one body prestressing the tendons after manufacturing the segmental girder, and second prestressing after the casting of the slab concrete. The test result shows that the measured values were almost same or slightly bigger than the theoretical values which means that the PSI girder bridges concept came out to be reliable.

  • PDF