• Title, Summary, Keyword: Marine microorganism

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Screening on Allogeneic Mixed Lymphocyte Culture Inhibitory Activity for the Extracts of Marine-Derived Microorganisms (해양미생물 추출물의 동종세포반응 (Allogeneic Mixed Lymphocyte Culture) 억제효능 검색)

  • Yun, Keum-Ja;Oh, Keun-Hee;Lee, Dong-Sup;Choi, Hong-Dae;Kang, Jung-Sook;Son, Byeng-Wha
    • Korean Journal of Pharmacognosy
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    • v.42 no.4
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    • pp.354-360
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    • 2011
  • In order to screen new allogeneic mixed lymphocyte culture (allo-MLR) inhibitor which is expected to be immunomodulating drug lead, we have investigated allo-MLR inhibitory activity on the marine-derived symbiotic microorganisms (1,895 strains) from the marine algae. The potent inhibitory activities (over 45% inhibition of proliferation at 10 and 2 ${\mu}g/ml$) without cytotoxicity were observed in the extracts of 46 strains. While, the significant stimulating activities (over 100% proliferation at 10 and 2 ${\mu}g/ml$) without cytotoxicity were observed in the extracts of 5 strains. In the second assay using 46 bioactive strains, 14 strains exhibited again significant allo-MLR inhibitory activity. Finally, 11 strains among the 14 strains inhibited proliferation and IFN-${\gamma}$ production of CD4+ T cells during the stimulation with specific antigen in the third assay. On the basis of above results, the marine algae is nice source for isolation of immunomodulating microorganism, and the marine algae-associated microorganism is also nice target for development of the new immunomodulating drug lead.

Development of an Hazard Analysis Critical Control Point (HACCP) Application Model for Dried Anchovy Workplace (마른멸치 작업장의 식품안전관리인증기준(HACCP)모델 개발)

  • YOON, Hyun-Jin;HAM, In-Tae;Kim, Sang-Hyeon;CHOI, Jong-Duck
    • Journal of Fisheries and Marine Sciences Education
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    • v.28 no.3
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    • pp.713-726
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    • 2016
  • This study were attempted to apply the HACCP system adopted in the dried anchovy workplace to ensure the hygiene safety of dried anchovy workplace. In this study, HACCP system procedures, including HACCP team organization, determination of critical control point(CCP), establishment of critical limits, mornitering and verification etc., were established using KFDA 12 procedures. To determine whether hazards were critical elements, we evaluated the likelihood and seriousness of each hazard element. The likelihood of residual microorganism and metal piece exceeding the acceptable limit in dried anchovy products were demonstrated to be a critical hazard element. Management of the boiling(CCP-1B) and metal detection(CCP-2P) were determined to be a critical control point. The standards for acceptable residual microorganism and metal piece were set based on relevant laws and regulations, and then the limits were adapted and established for the CCP. An HACCP plan applicable to dried anchovy workplace was established.

Degradation and Preservation of wood (목재문화재의 열화에 대한 고찰)

  • Kim, Ik-Ju
    • 보존과학연구
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    • pp.265-277
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    • 1986
  • The degradation of wood is maimly caused by biological and thermal factor. In general, the field of wood preservation can be divided into two broadcategories; namely the deterioration, protection of wood, and the teatment of wood with preservatives. Wood in sea or brackish water incurs marine borer damage, consisting of attack by marine animal and also wood on land suffers severely from insect damage. But the largest wood degradation is caused by microorganism. Animals that attack wood in a marine environment are especially destructive in warm water-regions, little was achieved in their control recently. Therefore this manuscript only introduce the importance of wood deterioration caused by marine animal.

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Microorganism lipid droplets and biofuel development

  • Liu, Yingmei;Zhang, Congyan;Shen, Xipeng;Zhang, Xuelin;Cichello, Simon;Guan, Hongbin;Liu, Pingsheng
    • BMB Reports
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    • v.46 no.12
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    • pp.575-581
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    • 2013
  • Lipid droplet (LD) is a cellular organelle that stores neutral lipids as a source of energy and carbon. However, recent research has emerged that the organelle is involved in lipid synthesis, transportation, and metabolism, as well as mediating cellular protein storage and degradation. With the exception of multi-cellular organisms, some unicellular microorganisms have been observed to contain LDs. The organelle has been isolated and characterized from numerous organisms. Triacylglycerol (TAG) accumulation in LDs can be in excess of 50% of the dry weight in some microorganisms, and a maximum of 87% in some instances. These microorganisms include eukaryotes such as yeast and green algae as well as prokaryotes such as bacteria. Some organisms obtain carbon from $CO_2$ via photosynthesis, while the majority utilizes carbon from various types of biomass. Therefore, high TAG content generated by utilizing waste or cheap biomass, coupled with an efficient conversion rate, present these organisms as bio-tech 'factories' to produce biodiesel. This review summarizes LD research in these organisms and provides useful information for further LD biological research and microorganism biodiesel development.

Production of Tetrodotoxin Using Novel Marine Microorganism Isolated from Intestine of Pufferfish (복어 장내에서 분리한 신규 해양 미생물을 이용한 Tetrodotoxin 생산)

  • 윤성준;송성광;이명자;정동윤;김희숙;김동수;이은열
    • Journal of Life Science
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    • v.10 no.2
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    • pp.196-201
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    • 2000
  • The production of tetrodotoxin (TTX) using Vibrio sp. YE-101, a novel marine microorganism isolated from the intestine of pufferfish, was investigated. Culture condition was optimized for the enhanced production of TTX using response surface methodology. The experimental sets of environmental conditions including pH, temperature and NaCl concentration were designed using central composite experimental design. The optimal conditions of pH, temperature and NaCl concentration were determined to be 8.1, 29.2℃, and 2.6% (w/v) respectively. The relative growth extent could be enhanced up to 80%, and final mouse unit (MU) value of TTX was also enhanced up to 87% by response surface optimization.

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The Effect of Aeration Rate on the Production of Exopolysaccharide, EPS-R by Marine Microorganism 96CJ10356

  • Lee, Hyeon-Sang;Park, Sin-Hye;Lee, Jong-Ho;Lee, Hong-Geum
    • 한국생물공학회:학술대회논문집
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    • pp.211-214
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    • 2000
  • Marine Microorganism strain 96CJ10356 produced extracellular polysaccharide (EPS-R) accompanied with cell growth. To improve the production of EPS-R, the effect of aeration rate was tested in a 5-liter jar fermentor with STN medium. The production of EPS-R was increased with aeration rate and after 72 hour cultivation, 12.20 g/l of EPS-R was obtained with an aeration rate of 1.5 vvm and the apparent viscosity was measured to be about 1000 cp with culture broth.

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Isolation and Identification of DHA-Rich Marine Microorganism (Docosahexaenoic acid (DHA)를 다량 함유하는 해양미생물의 분리 및 동정)

  • Jeong, U-Cheol;Choi, Byeong-Dae;Choi, Jong-Duck;Kang, Seok-Joong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.47 no.1
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    • pp.31-38
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    • 2014
  • Docosahexaenoic acid (DHA, 22:6n-3) and ecosapentaenoic acid (20:5n-3) have attracted increasing attention since the first epidemiological report on the importance of n-3 essential fatty acids. It is thought that DHA has important functions in brain and retinal tissues. Thraustochytrids, a group of marine protists, are capable of heterotrophic growth, and are potential omega-3 producers for industrial use, especially the members of the Schizochytrium and Thraustochytrium genera. The aims of this work were to isolate, identify and screen thraustochytrids from 17 different locations. Twenty-three isolates were screened for biomass, total fatty acid (TFA) and DHA content. Analysis of the fatty acid methyl esters revealed four distinct clusters biomass ranged from $8.68-9.36gL^{-1}$, and lipid and DHA contents ranged from $3.11-4.10gL^{-1}$ and $1.05-1.93gL^{-1}$ biomass, respectively. B-12 isolates were screened for biomass ($9.36gL^{-1}$), TFA ($4.10gL^{-1}$) and DHA (47.01%, w/w) content. C-6 isolates were also screened for biomass ($8.92gL^{-1}$), TFA ($3.30gL^{-1}$) and DHA (49.41%, w/w) content. The 18S rRNA gene sequencing results identified Schizochytrium mangrovei as B-12 and Crypthecodium cohnii as C-6.

Optimal Conditions for the Production of Exopolysaccharide by Marine Microoranism Hahella chejebsis

  • Ko, Sung-Hwan;Lee, Hyun-Sang;Park, Shin hye;Lee, Hong-Kum
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.5 no.3
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    • pp.181-185
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    • 2000
  • A marine microorganism, strain 96CJ10356 produced exopolysaccharide, designated as EPS-R. To optimize culmize culture conditions for the production of EPS-R, carbon and nitrogen sources, mineral salts, temperature, and pH were exmined. From this study, STN medium for the production of EPS-R was suggested as follows; sucrose 20g, typtone 10g, NaCl 10g, MgSO45g, CaCl21g, KH2PO4 76mg, K2HPO4 83mg, FeCl2 5mg, MnCl2 1mg, NaMoO4 1mg, and ZnCl2 1mg per liter at pH 7.0. About 9.23g/L of EPS-R was obtained from STN medium after cultivation for 120h at $25^{\circ}C$ in a 5-liter jar fermentor with an aearation rate of 0.17 vvm. Apparent viscosity and flocculation activity of the culture broth were increased with the production of EPS-R and the maximal values were 415 cP and 1400 unit/mL against 0.5% activated carbon, respectively.

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Effect of Culture Conditions on Characteristics of Growth and Production of Docosahexaenoic acid (DHA) by Schizochytrium mangrovei (배양조건에 따른 Schizochytrium mangrovei의 성장 및 Docosahexaenoic acid의 생산특성)

  • Jeong, U-Cheol;Choi, Byeong-Dae;Kang, Seok-Joong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.47 no.2
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    • pp.144-153
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    • 2014
  • Both docosahexaenoic acid (DHA, 22:6n-3) and eicosapentaenoic acid (EPA, 20:5n-3) have attracted increasing attention since the first epidemiological report on the importance of n-3 essential fatty acids. Lipids in microbial cells play various biological roles and, consequently, much research has been carried out on their role in cell physiology. The lipid composition of microorganisms can exhibit considerable variations depending on environment. The effects of culture conditions, temperature (15, 20, 24, 28, 32 and $36^{\circ}C$), salinity (10, 20, 30, 40 and 50 psu), pH (pH5, 6, 7, 8 and 9), rotation speeds (50, 100, 150 and 200 rpm), carbon sources, nitrogen sources and C/N ratio on the production of docosahexaenoic acid, fatty-acid profiles, and acids secreted to the broth culture by the oleaginous microorganism, Schizochytrium mangrovei (KCTC 11117BP), were studied. Temperature (initially $28^{\circ}C$), salinity (20 psu), pH (pH7), rotation speeds (100 rpm), organism fatty acids, and secreted acids in the broth were varied during cultivation of S. mangrovei. At pH 7.0, S. mangrovei was able to accumulate lipids up to 40% of its biomass, with 13% (w/w) DHA content. The monosaccharides glucose and fructose, and yeast extract were suitable carbon and nitrogen sources, respectively. The primary omega-3 polyunsaturated fatty acid produced was docosahexaenoic acid.

Evaluation of the Biological Activities of Marine Bacteria Collected from Jeju Island, Korea, and Isolation of Active Compounds from their Secondary Metabolites

  • Kim, Hyun-Soo;Zhang, Chao;Lee, Ji-Hyeok;Ko, Ju-Young;Kim, Eun-A;Kang, Nalae;Jeon, You-Jin
    • Fisheries and aquatic sciences
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    • v.17 no.2
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    • pp.215-222
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    • 2014
  • To explore marine microorganisms with medical potential, we isolated and identified marine bacteria from floats, marine algae, animals, and sponges collected from Jeju Island, Korea. We isolated and identified 21 different strains from the marine samples by 16S rRNA analysis, cultured them in marine broth, and extracted them with ethyl acetate (EtOAc) to collect secondary metabolite fractions. Next, we evaluated their anti-oxidative and anti-inflammatory effects. Among the 21 strains, the secondary metabolite fraction of Bacillus badius had both strong antioxidant and anti-inflammatory activity, and thus was selected for further experiments. An antioxidant compound detected from the secondary metabolite fraction of B. badius was purified by preparative centrifugal partition chromatography (n-hexane:EtOAc:methanol:water, 4:6:4:6, v/v), and identified as diolmycin A2. Additionally, diolmycin A2 strongly inhibited nitric oxide production. Thus, we successfully identified a significant bioactive compound from B. badius among the bacterial strains collected from Jeju Island.