• Title, Summary, Keyword: Marphysa sanguinea

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Environmental Characteristics on Larval Release of Rockworm Polychaete Marphysa sanguinea (바위털갯지렁이(Marphysa sanguinea) 유생 방출에 미치는 환경특성)

  • Kim, Kyeong-Hun;Vo, Thi Thu Em;Kim, Hong Jin;Kim, Chang-Hoon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.49 no.4
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    • pp.486-492
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    • 2016
  • This study investigates the relationship between environmental factors, such as semilunar rhythm and atmospheric pressure, and the release of Marphysa sanguinea larvae. During artificial seed production in a temperature-regulated culture system, there was an increase in the number of released larvae at tide times between 3-4 and 9-11. Numbers of larvae released were significantly correlated with tidal rhythms. Atmospheric pressure also appeared to influence larval release, with increased numbers released when a period of high atmospheric pressure followed a period of low pressure.

Effects of Substrate Sizes and Organic Contents on Larval Settlement and Growth in the Early Stage of the Polychaete Marphysa sanguinea (바위털갯지렁이(Marphysa sanguinea) 유생의 착저와 초기 성장에 미치는 기질 크기 및 유기물 함량)

  • Phoo, War War;Kim, Sung Kyun;Kim, Chang-Hoon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.53 no.1
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    • pp.132-138
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    • 2020
  • Marphysa sanguinea is the most well-known polychaete species with a high economic value. However, this species has a high mortality in the early rearing stage of aquaculture. This study was conducted to find out the optimal substrate size and organic contents for the growth and survival rate of M. sanguinea larvae and juveniles. It was observed that the smaller grain size (<Ø 0.063 mm) and high organic contents (5-10%) induced settlement and reduced settlement time of larvae. Moreover, the growth and survival rate of larvae reached high levels at Ø 0.004-0.016 mm of grain sizes and 5-7.5% of organic contents as advantageous substrates for settlement. The survival rate of juveniles reached over 90% in less than Ø 0.016 mm substrate on 15-day experiment for different grain sizes of substrates. These results indicated that substrate compositions of less than Ø 0.016 mm of sand size and 5-7.5% of organic contents in mud will enhance the productivity of M. sanguinea at the early stage.

Identification of a new serine protease from polychaeta, Marphysa sanguinea, for its thrombolytic and anticoagulant activity

  • Yeon, Seung Ju;Shim, Kyou Hee;Hong, Jae Sang;Shin, Hwa Sung
    • Korean Journal of Chemical Engineering
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    • v.34 no.3
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    • pp.781-786
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    • 2017
  • A serine protease was purified from Marphysa sanguinea through ammonium sulfate followed by ion exchange chromatography, and its N-terminal amino sequence was identified to be IVGGSEATPYQFPFQ. Fibrinolytic activity was depended on both direct fibrinolysis and indirect plasminogen-mediated cascade and had a consistent activity irrespective of pH. The serine protease could be confirmed to degrade ${\alpha}$-, ${\beta}$-, and ${\gamma}$-chains of human fibrinogen through fibrinogenolytic assay and did not express significant cytotoxicity to endothelial cells. These imply the enzyme has anticoagulant as well as thrombolytic activity, not significantly impairing endothelial cells comprising brain blood brain barrier (BBB) tissue. Conclusively, the new serine protease is worthy of being a candidate to substitute tissue-Plasminogen Activator (t-PA) for acute ischemic reperfusion injury of brain.

Effects of Rearing Conditions on the Artificial Seed Production of a Polychaete Marphysa sanguinea (바위털갯지렁이 Marphysa sanguinea의 인공종묘생산에 미치는 사육환경의 영향)

  • Kim, Chang-Hoon;Jang, Sung-Wook
    • Journal of Aquaculture
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    • v.21 no.1
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    • pp.34-40
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    • 2008
  • This study investigated the effect of rearing conditions on the spawning habit, juvenile growth and larvae development of Marphysa sanguinea, to develop techniques of its artificial seed production. M. sanguinea released trochophore larvae around the burrow when the water temperature was from $18^{\circ}C$ to $22^{\circ}C$. Larvae floated away after staying in the burrow for a while. Larvae secreted mucus eight days after release, covering their whole body with it, and developed ten somites 20 days after release. In preference of juveniles in different grain sizes of sediment, the highest survival rate was made in the grain substrate of $1{\sim}2$ mm in the mean diameter, but the lowest was in the $2{\sim}3$ mm grain substrate. Optimum growth was obtained at the rearing temperature of $24^{\circ}C$(SGR 1.10%), and it reached over $2{\sim}3$ times higher weight gain and specific growth rate than those reared at $21^{\circ}C(0.64),\;18^{\circ}C(0.56)$ and $15^{\circ}C$(0.42) for 50 days. Salinity also made a great difference in the growth and survival rate. The highest weight gain and growth rate were shown when the juveniles were reared at salinity 30 psu.

Effects of Rearing Density on Growth of the Polychaete Rockworm Marphysa sanguinea

  • Parandavar, Hossein;Kim, Kyeong-Hun;Kim, Chang-Hoon
    • Fisheries and aquatic sciences
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    • v.18 no.1
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    • pp.57-63
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    • 2015
  • Effects of rearing density on growth and survival of the polychaete rockworm Marphysa sanguinea have been investigated in order to develop rearing techniques for this species. This study was examined over a nine-month period in the Fisheries Science and Technology Center of Pukyong National University. Three rockworm densities, 500, 1,000 and $2,000worms{\cdot}m^{-2}$ with weight ranges of < 0.5 g, 0.6-1.5 g, and 1.6-2.5 g, and the no feed control treatment, were stocked in triplicate $0.10m^2$ boxes with sand bottoms. Growth rates were checked with 15 randomly sampled rockworms from each box at months 3, 6 and 9. Results showed that SGRs in all treatments were higher during the first period (0-3 months) than the second (3-6 months) and third periods (6-9 months) for all treatment densities, while SGRs decreased with increasing density. However, survival and growth of worms at high density was not better than low density, but daily biomass production in medium and high density groups was $6.28gm^{-2}day^{-1}$ for the rockworms of 0.6-1.5 g with $2,000inds{\cdot}m^{-2}$, and $12.6gm^{-2}day^{-1}$ for group between 1.6-2.5 g with $2,000inds{\cdot}m^{-2}$, and $14.7gm^{-2}day^{-1}$ for the group of individuals <0.5 g with $1,000inds{\cdot}m^{-2}$. Results showed that M. sanguinea can be one of the most suitable species to commercially exploit in a farming system. In particular, specified densities permit elevated pure production.

Study on the Acceptance Level for Damage due to Underwater Noise from the Case Study of Field Measurement at Marphysa sanguinea Farm (바위갯지렁이 양식장 인접지역 계측 사례를 포함한 수중소음 피해인정기준에서 배경소음과의 차에 대한 문제점)

  • Choi, Tae-Hong;Kim, Jae-Woong;Ko, Chin-Surk
    • Explosives and Blasting
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    • v.35 no.1
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    • pp.34-42
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    • 2017
  • It is very difficult to clearly define the damages caused by blasting-induced noise and vibration, because the damages depend on, besides the level of noise and vibration, the response of the object, environmental conditions, subjective feeling, and mental condition. Especially, it is more difficult when the fish is concerned, because that experimental approach is not easy and that we lack of the reasonable criterion for the acceptance level of noise and vibration. In Korea, the acceptance level for damage due to underwater noise is 140 dB re $1{\mu}Pa$, and the difference from the underwater background sound level is defined as more than 20 dB re $1{\mu}Pa$. It is however, appropriate for continuous noise not for transient sound. The authors compared the relationship between vibration velocity and underwater noise measured from the test blasting around Marphysa sanguinea farm. This paper presents the measurement results and suggestions the acceptance level for damage due to underwater noise from explosive blasting.

Antioxidant and Anti-Inflammatory Effects of NCW Peptide from Clam Worm (Marphysa sanguinea)

  • Park, Young Ran;Park, Chan-Il;Soh, Yunjo
    • Journal of Microbiology and Biotechnology
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    • v.30 no.9
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    • pp.1387-1394
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    • 2020
  • Clam worms (Marphysa sanguinea) are a rich source of bioactive components such as the antibacterial peptide, perinerin. In the present study, we explored the physiological activities of a novel NCWPFQGVPLGFQAPP peptide (NCW peptide), which was purified from clam worm extract through high-performance liquid chromatography. Tandem mass spectrometry (MS/MS) revealed that NCW was a new peptide with a molecular weight of 1757.86 kDa. Moreover, NCW peptide exhibited significant antioxidant effects, causing a 50% inhibition of DPPH radical at a concentration of 20 μM without showing any cytotoxicity. These were associated with a reduction in the activity of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) in LPS-stimulated RAW264. 7 cells. Furthermore, NCW peptide exhibited anti-inflammatory effects in LPS-stimulated RAW264.7 macrophages via inhibition of the abnormal production of pro-inflammatory cytokines including nitric oxide (NO), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). These anti-inflammatory effects of NCW peptide were associated with the inhibition of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). Our results therefore suggest that this novel NCW peptide with antioxidant and anti-inflammatory effects could be a good therapeutic agent against inflammation-related diseases.

Anti-inflammatory and Antioxidant Effects of Clam Worm Extract Treated with Peptidoglycan (펩티도글리칸 처리된 갯지렁이 추출물의 항염증 및 항산화 효과)

  • Kim, Se-woong;Sapkota, Mahesh;Yang, Ming;Li, Liang;Soh, Yunjo
    • Korean Journal of Pharmacognosy
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    • v.48 no.3
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    • pp.187-194
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    • 2017
  • Peptidoglycan in inserts and mammals is well known to improve biological functions in the host's immune system. However, it is unclear how Peptidoglycan exerted its anti-inflammatory capacity especially in clam worm (Marphysa sanguinea). In this experiment, the anti-inflammatory and antioxidant effects of clam worm extract treated with (PCWE) peptidoglycan (Micrococcus luteus) in RAW264.7 cells were examined by measuring MDA, catalase, SOD, GSH-Px and inflammatory cytokines (nitric oxide, iNOS, interleukin-$1{\beta}$ and tumor necrosis factor-${\alpha}$). PCWE significantly increased the activities of catalase, SOD and GSH-Px and decreased the level of MDA. Interestingly, PCWE induced activities of SOD and GSH-Px more than clam worm extract without peptidoglycan (CWE). In addition, PCWE decreased NO production, iNOS, COX-2, TNF-${\alpha}$ and IL-$1{\beta}$ better than CWE. Taken together, these results indicate that PCWE has the potential as a natural antioxidant and a therapeutic for inflammation-related diseases.