• Title, Summary, Keyword: Medicinal Plant

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The Branch Extracts of Vaccinium oldhamii Stimulate Melanin Synthesis Through Activation of Tyrosinase Activity in B16F10 Melanoma Cells

  • Park, Su Bin;Kim, Ha Na;Kim, Jeong Dong;Park, Gwang Hun;Son, Ho-Jun;Eo, Hyun Ji;Song, Jeong Ho;Jeong, Hyung Jin;Jeong, Jin Boo
    • Proceedings of the Plant Resources Society of Korea Conference
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    • pp.95-95
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    • 2018
  • In this study, we investigated the effect of branch extracts from Vaccinium oldhamii (VOB) on melanin synthesis in B16F10 cells. VOB promoted melanin production in absence or presence of ${\alpha}$-melanocyte-stimulating hormone (${\alpha}-MSH$) in B16F10 cells. However, VOB did not affect the expression of tyrosinase and TRP-1 associated with melanin synthesis at the mRNA and protein level in B16F10. But, VOB decreased TRP-2 protein level and induced tyrosinase activation in B16F10 cells. Inhibition of tyrosinase activity and tyrosinase knockdown attenuated VOB-mediated melanin synthesis. In conclusion, it is thought that VOB may stimulate melanin synthesis through activating tyrosinase activity.

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Enhancement of Melanin Synthesis by the Branch Extracts of Vaccinium oldhamii through Activating Tyrosinase Activity in B16F10 Melanoma Cells

  • Son, Kun Ho;Baek, Jueng Kyu;Park, Su Bin;Kim, Ha Na;Park, Gwang Hun;Son, Ho-Jun;Eo, Hyun Ji;Song, Jeong Ho;Jeong, Hyung Jin;Jeong, Jin Boo
    • Korean Journal of Plant Resources
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    • v.31 no.5
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    • pp.547-553
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    • 2018
  • This study was conducted to investigate the effect of branch extracts of Vaccinium oldhamii (VOB) on melanin synthesis in B16F10 cells. VOB promoted melanin production in absence or presence of ${\alpha}$-melanocyte-stimulating hormone (${\alpha}-MSH$) in B16F10 cells. However, VOB did not affect the expression of tyrosinase and TRP-1 associated with melanin synthesis at the mRNA and protein levels in B16F10. But, VOB decreased TRP-2 protein level and induced tyrosinase activation in B16F10 cells. Inhibition of tyrosinase activity and tyrosinase knockdown attenuated VOB-mediated melanin synthesis. In conclusion, VOB may stimulate melanin synthesis through activating tyrosinase activity.

In vitro propagation of Coleus forskohlii, an important medicinal plant

  • Yang, Deok-hun;Jeon, Manju-Meluttu-George;Jong-Seong
    • Plant Resources
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    • v.6 no.2
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    • pp.129-133
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    • 2003
  • For mass multiplication of an important medicinal plant Coleus forskohlii, a procedure for the high frequency regeneration of Coleus forskohlii has been developed using leaf explants via callus culture. Callus formation occurred in MS medium supplemented with 1-2 mg/L each of NAA and BAP. A large number of shoots were formed on MS + 1 mg/L BAP from 50-60 days old greenish calli. Rooting of healthy shoots occurred on 0.1-0.4 mg/L NAA. The protocol described could be useful in future for genetic manipulation of this plant species.

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