• 제목, 요약, 키워드: Medium development

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Effect of MEM Vitamins Supplementation of In vitro Maturation Medium and In vitro Culture Medium on the Development of Porcine Embryos

  • Kim, J.Y.;Lee, E.J.;Park, J.M.;Park, H.D.
    • 아세아태평양축산학회지
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    • v.24 no.11
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    • pp.1541-1546
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    • 2011
  • This study was carried out to examine the influence of minimum essential medium (MEM) vitamins supplementation to in vitro maturation medium and in vitro culture medium on the development of porcine embryos. Porcine embryo development was investigated following cultivation in both in vitro maturation and culture medium with the supplementation of MEM vitamins (0, 0.1, 0.2 and 0.4%) using immature oocytes collected from the ovary of prepubertal gilts. Embryo development was observed and the total cell number in each blastocyst generated under the culture conditions was quantified following supplementation of the medium. The embryonic development rate of the blastocyst and hatched blastocyst was higher, but not significantly so, when 0.4% MEM vitamins were supplemented to the in vitro maturation medium of the porcine oocyte. Interestingly, the total number of cells in the blastocyst was significantly higher in the in vitro maturation MEM vitamins supplemented group compared to either the untreated group or the group which had MEM vitamins supplemented to both in vitro maturation and in vitro culture medium simultaneously (p<0.05). Therefore, the supplementation of 0.4% MEM vitamins to the in vitro mature medium has a beneficial effect on the embryonic development of in vitro produced blastocysts derived from the immature porcine oocytes.

토끼 수정란 체외 배양액의 개발에 관한 연구 (Study on Development of In Vitro Culture Medium for Rabbit Embryos)

  • 임경순;진동일;김대경;김성우;정소용;최화식
    • 한국가축번식학회지
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    • v.22 no.1
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    • pp.35-42
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    • 1998
  • This experiment was carried out to improve in vitro development of rabbit one-cell embryos to the blastocyst stage. One-cell rabbit embryos were collected at 19\ulcorner20hr after superovulation induction and incubated at 39\ulcorner in 5% CO2 for 72hr. In order to find optimum conditions in medium that affects the rabbit embryo's development in vitro, RDH medium which mixed with RPMI1640, DMEM and Ham's F10 was compared with the previously reported mediums (Ham's F10 and RD) for embryo development and cell numbers. Three additives (BSA, taurine and glucose) were tested for the development of rabbit one-cell embryos in vitro. When the embryos were cultured in RDH medium, their development was markedly promoted as compared with Ham's F-10 or RD alone. Glucose exhibited no significant effects on embryo development and cell numbers. BSA a, pp.ared to promote transition from morula to blastocyst stage and taurine increased cell numbers of cultured embryos markedly regardless of medium. BSA and taurine together in RDH medium showed the additive effects on embryos development and cell number.

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중소규모 SW개발 프로젝트를 위한 시각화 기반의 Tool-Chain 품질관리 방법 제안 (A Method for Tool-Chain-driven Quality Control based on Visualization for Small and Medium Scale Software Development Projects)

  • 김정보;정진영;김정인
    • 한국멀티미디어학회논문지
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    • v.18 no.4
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    • pp.546-556
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    • 2015
  • Since the concept of software engineering was first used in 1968 by NATO Science Committee, a lot of research work and improvements have been made on software development methodology and software quality control, but they still fall short of ensuring successful development of small and medium scale software systems. Under these circumstances, Center for Software Engineering (CSE) at National IT Industry Promotion Agency(NIPA) has been conducting studies on quality control methodologies of software visualization well-suited for small and medium scale software systems, and also working on the systemization and quantification of software quality control. In this paper, we attempt to scope on the software development management of domestic and foreign small and medium-sized enterprises that are lying in the blind spot, compared to large enterprises with well-organized software development systems. In particular, based on software visualization that CSE is pursuing for small and medium-sized developers, we propose a practical quality control methodology well-suited for small and medium scale projects, and a low-cost quality control management tool by combining open-source quality control tools. Our proposal is expected to induce developers' mind change in SI-specialized small and medium-sized software enterprises, increase their profits and improve customer satisfaction through project quality control.

Effect of Glucose and Sodium Phosphate on In Vitro Development of Porcine Embryos

  • Lee, S.H.;Lim, S.M.;Lee, S.Y.;Cheong, H.T.;Yang, B.K.;Park, C.K.
    • Reproductive and Developmental Biology
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    • v.28 no.2
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    • pp.101-105
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    • 2004
  • This study was carried out to evaluate the effects of glucose and sodium phosphate on in vitro development of porcine oocytes matured and fertilized in vitro. When the culture medium was supplemented with various concentrations of glucose, the higher proportions (23 and 26%) of oocytes developed to morular or blastocyst stages were at the concentrations of 2.78 and 5.56 mM than 0 (9%; P<0.05) and 11.12 mM (18%). In experiment to evaluate effect of sodium phosphate during in vitro development of porcine oocytes, a significantly (P<0.05) higher proportions of embryos developed to morular or blastocyst stages was obtained with sodium phosphateof 0.28 (25%) and 0.53 (27%) mM than 0 (15%), 1.05 (19%) and 2.10 (10%) mM. On the other hand, when oocytes were cultured in medium with (0.53 mM) sodium phosphate, the proportions of developed embryos were significantly (P<0.05) higher in medium without (29%) that than with (14%) 5.56 mM glucose. However, a higher proportion of embryos developed to morular or blastocyst stages were obtained in medium with (23%) that than without (8%) glucose (P<0.05). The minimum essential medium (MEM) added to the culture medium were higher regardless of presence of sodium phosphate and glucose on the development of embryos. Although sodium phosphate and glucose could support morular and blastocyst development to a limited extend (10∼24%), significantly higher proportion (36%) at morular or blastocyst stages was obtained by MEM adding in the medium with sodium phosphate and glucose. These results suggest that the early development of in vitro fertilized porcine oocytes can be maintained efficiently by glucose and sodium phosphate when they were cultured in medium with MEM.

생쥐난자의 시험관내 수정과 발달 (In Vitro Fertilization and Development of Mouse Eggs)

  • 김승재;정길생
    • 한국가축번식학회지
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    • v.8 no.2
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    • pp.110-115
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    • 1984
  • These experiments were carried out to obtain the information about the optimal pH osmolality affecting in vitro fertilization of the mouse eggs, to elucidate the 2-cell block to development in vitro and to find out the method of controlling the subsequent embryo development in vitro. pH and osomlality was adjusted by adding NaCl or NaHCO3 to the basic salt solution. In vitro fertilization were carried out by inroducting the cumulus masses to the suspension of epididymal spermatozoa at each pH, osmolality, and 10${\mu}$M-EDTA medium. The results obtained in these experiments were summarized as follows: 1. The fertilization rates in vitro at each medium of 235, 252, 269, 286, 306, 323, 345, 368, 393 mosmol were 15.6, 38.2, 65.7, 75.6, 80.9, 74.3, 58.1, 35.1, 24.3, 11.1%, respectively. 2. The fertilization rates in vitro at each medium of pH 6.1, 6.4, 6.7, 7.0, 7.3, 7.6, 7.9, 8.1 were 11.8, 17.9, 32.4, 61.9, 79.5, 76.7, 53.5, 13.6%, respectively. 3. In case of ICR female x ICR male embryos, the development rate of 2-cell embryos to 4-8 cell embryos was 16.2% at normal medium, but the rate was increased to 49.3% in medium containging 10 ${\mu}$M-DETA; In case of C3H female x ICR male embryos, the development rate was 41.0% at normal medium, but the rate was increased to 71.7% at 10 ${\mu}$M-EDTA-medium.

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돼지 난관액과 Oviductal Conditioned Medium 이 다정자침입과 체외배발달에 미치는 영향 (Effect of Oviductal Fluid and Oviductal Conditioned Medium on Polyspermy and In Vitro Development of Porcine Oocytes)

  • 문승주;김재홍;나진수
    • 한국가축번식학회지
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    • v.22 no.4
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    • pp.411-417
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    • 1998
  • 본 연구는 난관액과 oviductal conditioned medium 이 다정자 침입과 체외배발달에 미치는 효과를 규명하기 위하여 수행하였다. 배양액내 난관액과 oviductal conditioned medium 의 첨가는 다정자침입율과 난자내 침입한 평균정자수를 감소시켰다 (P<0.05). 정자와 난관액 그리고 oviductal conditioned medium 과 l.5, 3, 4.5 시간 공배양후 첨체반응의 성적은 대조구에 비하여 증가하였다. 체외수정후 체외발달 배양액에 난관액이나 oviductal conditioned medium 을 첨가하여 192 시간동안 배양했을 때 상실배와 배반포배발달율이 난관액과 oviductal conditioned medium 첨가구에서 유의적으로 높았다 (P<0.05). 이러한 연구결과는 난관액과 OVCM 등 난관 유래물질은 다정자침입율과 난자내 침입한 평균정자수를 감소시키며 체외발달율을 높인다고 사료된다.

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제지 부산물을 이용한 느타리버섯(Pleurotus ostreatus) 자실체 형성용 염가배지개발 (Development of Cheap Substrate for Fruiting of Pleurotus ostreatus using Paper Sludge)

  • 조우식;윤영석;박선도;최부술
    • 한국균학회지
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    • v.23 no.3
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    • pp.197-201
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    • 1995
  • 느타리버섯 재배에 있어서 배지재료로 제지부산물의 사용 가능성을 검토한 결과 배지재료별 화학특성은 제지부산물이 폐면, 볏짚, 왕겨보다 CaO는 많았고, T-C, T-N, $P_2O_5$, MgO는 비슷하였으며 $K_2O$는 현저히 적었다. 균사생장은 혼합처리가 폐면단용보다 생장이 다소 빠르거나 비슷한 경향이었다. 초발이소요일수는 폐면단용보다 혼합처리가 비슷하거나 $1{\sim}2$일 정도 빠른 경향이었다. 배지종류별 수량은 폐면단용에 비해 폐면 10+왕겨 20+제지부산물 70% 처리를 제외하고는 증수되었으며, 특히 계면+제지부산물 30% 혼합처리가 21% 증수되어 가장 우수하였다. 경제성분석결과 폐면단용보다 폐면+제지부산물 30% 혼합처리에서 소득지수가 50% 높게 나타났다.

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Growth and Differentation of Rat Mammary Epithelial Cells Cultured in Serum-free Medium

  • Kim, Dong-Yeum;Jhun, Byung-Hak;Lee, Kyung-Hee;Hong, Seung-Chul;Clifton, Kelly-H.;Kim, Nam-Deuk
    • Archives of Pharmacal Research
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    • v.20 no.4
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    • pp.297-305
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    • 1997
  • A new serum-free defined medium was developed that supports the growth of normal rat mammary epithelial cells. Mammary organoids from the glands of female F344 rats were cultured in a serum-free medium. Monolayer culture colonies developed within a week and remained viable for months in culture. Upon subculture of one-week-old primary colonies, almost the same morphology of colonies was developed. The scrape loading/dye transfer technique showed that most of colonies that developed in a serum-free medium containing EGF, human transferrin, insulin, and hydrocortisone (basal serum-free medium, BSFM) failed to show cell-cell communication. However, colonies cultured in BSFM supplemented with prolactin, $E_2$, and progesterone (complete hormone serum-free medium, CHSFM) showed cell-cell communication at 14 days of primary culture or of subculture. By flow cytometry with FITCPNA and PE-anti-Thy-1.1 monoclonal antibody, we distinguished four RMEC subpopulations in cultures in both media: Thy-1.1+ cells, PNA+ cells, cells negative to both reagents and cells positive to both reagents. It is likely that combined prolactin, cortisol, and insulin in CHSFM stimulate terminal differentiation of clonogenic cells.

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Plant Regeneration from Shoot Tip-Derived Embryogenic Callus of Dianthus superbus

  • Lee, Eun-Ae;Kim, Joon-Chul;Kim, Won-Bae;Kim, Byeong-Hyeon;Kim, Jeong-Kan
    • Journal of Plant Biology
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    • v.37 no.3
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    • pp.381-385
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    • 1994
  • The highest degree of callus formation was obtained from the shoot tips of Dianthus superbus when cultured on the MS medium supplemented with 2.0 mg/L NAA and 0.5 mg/L BAP. Embryogenic calluses were obtained from the seperated friable calluses on MS medium containing 2.0 mg/L 2,4-D after 7-8 wk of culture. For plant regeneration, embryogenic calluses were selected and cultured on te proliferation medium. After 3 wk, somatic embryos appeared on MSK medium (0.5 mg/L NAA, 2.0 mg/L kinetin) and N6 medium (2.0 mg/L kinetin, 0.1 mg/LNAA, 0.1 mg/L 2,4-D and 2.0 g/L casein hydrolysate). When these somatic embryos were kept under continuous illumination, shoots were successfully regenerated on the both media. The shoots were rooted on MS medium supplemented with 2.0 mg/L NAA.

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난관상피세포 Conditioned Medium이 체외수정된 소 수정란의 체외 발달에 미치는 영향 (Effect of Bovine Oviductal Epithelial Cell(BOEC) Conditioned Medium on In Vitro Development of Bovine Embryos Fertilized In Vitro)

  • 오종훈;김동훈;정형민;이훈택;정길생
    • 한국가축번식학회지
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    • v.17 no.1
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    • pp.69-74
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    • 1993
  • This study was investigated to examine the effect of conditioned medium from bovine oviductal cell(BOEC) in the co-culture system with BOEC on in vitro development of in vitro produced bovine embryos. Oocyte-cumulus complexes were cultured for 24 hrs in TCM-199 supplemented with 10% fetal calf serum, 1$\mu\textrm{g}$/ml FSH and 21U hCG, 1$\mu\textrm{g}$/ml oestradiol-17$\beta$ at 39$^{\circ}C$ under 5% CO2 in air. In vitro fertilization was performed with epididymal sperm and heparin (10$\mu\textrm{g}$/ml, 15min.) or caffeine(2.5mM)-treated spermatozoa. Oocytes were incubated with 1$\times$106 spermatozoa/ml for 18 hrs and then cultured in various culture system for 7 days. The development rates of 16-cell or blastocyst stages were recorded on 4, 7 days, respectively, after incubating. The proportions ofembryonic development into molulae and blastocysts were higher in cumulus cell co-culture(23.4%) and BOEC co-culture(34.3%) than in M199-FCS(6.1%). Similarily, the development rates into molulae and blastocysts were significantly higher in BOEC-conditioned medium than those in M199-FCS. Therefore, it is suggested that BOEC co-culture and BOEC conditioned medium increase significantly the development of in vitro produced bovine embryos in in vitro system.

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