• Title, Summary, Keyword: Medium development

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Development of Carrot Medium Suitable for Conidia Production of Venturia nashicola

  • Choi, Eu Ddeum;Kim, Gyoung Hee;Lee, Young Sun;Jung, Jae Sung;Song, Jang Hoon;Koh, Young Jin
    • The Plant Pathology Journal
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    • v.33 no.1
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    • pp.75-79
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    • 2017
  • The causal fungus of pear scab, Venturia nashicola, grows slowly and rarely produces conidia on artificial media in the laboratory, but it produced conidia on the Cheongah medium containing Cheongah powder. V. nashicola grew too slow to produce conidia until 15 days after cultivation but produced conidia with $4{\time}10^4$ conidia/plate 30 days after cultivation on the Cheongah medium containing 1% Cheongah powder. V. nashicola showed a peak production of conidia with $4.5{\times}10^5$ conidia/plate 60 days after cultivation on the carrot medium containing 2% carrot powder, one of the constituents of Cheongah powder. The carrot medium is considered to be the best medium to obtain conidia of V. nashicola in the laboratory until now. This is the first report on the development of a suitable medium for conidia production of V. nashicola, as far as we know.

Effects of Sodium Chloride and Macromolecules in Chemically Defined Culture Medium on In Vitro Development of Bovine Embryos (단순한정배양액 내의 Sodium Chloride 및 Macromolecules가 소 수정란의 체외발육에 미치는 영향)

  • 노상호
    • Journal of Embryo Transfer
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    • v.15 no.2
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    • pp.191-196
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    • 2000
  • the present study was carried out to develop a completely defined culture system and determine if high NaCl concentrations in defined (PVA added) or semi-defined (BSA added) medium is toxic to bovine embryos. Oocytes from slaughterhouse ovaries were matured and fertilized in vitro. After 30 h of insemination, only 2-cell stage embryos were selected and cultured for this experiment. The culture media used were as follows : TLP(114 mM of NaCl) + BSA (3 mg/ml), TLP + PVA (1 mg/ml), mTLP(96 mM of NaCl) + BSA, mTLP + PVA. Six to ten embryos were placed into a 30$\mu$1 drop of each medium and the embryos were examined at 10 day post-insemination without medium renewal. The experiment was replicated 4 times. All data were analyzed by chi-square. There were no significant differences among TLP-BSA, mTLP-BSA and mTLP-PVA in blastocyst development (21.6, 17.2 and 20.2%), respectively. Also, no differences were obtained in hatching rates (11.7, 9.9 and 12.2%), respecitively. However, there were significant differences between TLP-PVA (1.7% and 0.6%) and other group in blastocyst formation and hatching rates, respectively (p<0.01). Development of in vitro produced embryos cultured in BSA containing medium was not affected by high NaCl concentration, but in the completely defined medium, embryonic development was highly affected by NaCl. This study shows that reduced NaCl concentration in completely defined medium is beneficial for development of bovine pre-implantation embryos in vitro.

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Effects of Insulin, Transferrin and Selenium (ITS) on In Vitro Development of Porcine Parthenogenetic and Nuclear Transfer Embryos

  • Quan, Yan-Shi;Naruse, Kenji;Kim, Baek-Chul;Kim, Hong-Rye;Han, Rang-Xun;Choi, Su-Min;Park, Chang-Sik;Jin, Dong-Il
    • Reproductive and Developmental Biology
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    • v.31 no.4
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    • pp.261-265
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    • 2007
  • Insulin, transferrin and selenium (ITS) complex is reported to improve in vitro development of oocytes and embryos. This study was carried out to investigate the effects of ITS during in vitro culture (IVC) of porcine parthenogenetic and nuclear transfer (NT) embryos on subsequent developmental capacity in vitro. The electrically activated oocytes were cultured in Porcine Zygote Medium (PZM-3) with various concentrations (0, 0.1, 0.5, and 1.0%) of ITS for 7 days. Also, the electrically activated reconstructed embryos were cultured in PZM-3 with various concentrations (0, 0.1, 0.5, and 1.0%) of ITS for 6 days. Addition of ITS to culture medium did not affect development of porcine parthenogenetic embryos in vitro. To test the effect of ITS on the in vitro development of porcine NT embryos, factorial experiments were also performed for in vitro maturation (IVM) medium (TCM-199) with or without 1% ITS and culture medium (PZM-3) with or without 0.5% ITS. Addition of 0.5% ITS to culture medium increased (p<0.05) the proportion of NT blastocysts compared with non-treated group. In contrast, addition of 1% ITS to culture medium was ineffective or had a detrimental effect. Also, addition of ITS only to maturation medium increased (p<0.05) the percentage of NT blastocysts formation compared with the control group. In conclusion, addition of ITS to IVM or IVC medium could improve subsequent blastocyst development of porcine NT embryos.

Detrimental Effect of Bovine Serum Albumin in a Maturation Medium on Embryonic Development after Somatic Cell Nuclear Transfer in Pigs

  • Lee, Hanna;Lee, Yongjin;Park, Bola;Elahi, Fazle;Lee, Joohyeong;Choi, Jung Hoon;Lee, Seung Tae;Park, Choon-Keun;Hyun, Sang-Hwan;Lee, Eunsong
    • Journal of Embryo Transfer
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    • v.29 no.4
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    • pp.361-368
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    • 2014
  • This study was designed to evaluate the effect of bovine serum albumin (BSA) in a maturation medium on oocyte maturation and embryonic development in pigs. Immature pig oocytes were matured for 44 h in a medium supplemented with 0.4% (w/v) BSA, 0.1% (w/v) polyvinyl alcohol (PVA), or 10% (v/v) pig follicular fluid (PFF). After IVM, oocytes reached metaphase II stage were activated for parthenogenesis (PA) or used as cytoplasts for somatic cell nuclear transfer (SCNT). Nuclear maturation (89.5%, 90.7% and 91.3% for BSA, PVA and PFF, respectively) and intraoocyte glutathione contents (1.20, 1.16 and 1.00 pixels/oocyte for BSA, PVA and PFF, respectively) were not altered by the macromolecules added to maturation medium. IVM of oocytes in a medium containing BSA (21.4%) and PVA (20.7%) showed significantly lower blastocyst formation after PA than culture in medium with PFF (39.2%). After SCNT, oocytes matured in medium with BSA showed decreased embryonic development to the blastocyst stage (9.2%) compared to those matured in medium with PFF (28.9%), while 23.6% of SCNT oocytes matured in medium with PVA developed to the blastocyst stage. When the effect of BSA in a maturation medium during the first 22 h and the second 22 h of IVM in combination with PFF or PVA was examined, PVA-BSA showed a higher nuclear maturation (94.1%) than BSA-PFF (84.5%). However, there was no significant difference in the blastocyst formation among tested combinations (47.3, 52.2, 50.0, 44.4 and 49.0% for PFF-PFF, PFF-BSA, PVA-BSA, BSA-PVA and BSA-PFF, respectively). Our results demonstrate that BSA and PVA added to maturation medium can support oocyte maturation comparable to PFF-supplemented medium. However, maturation of oocytes in a BSA-containing medium decreases embryonic development after PA and SCNT when compared with the medium supplemented with PFF.

Effect of EGF on In Vitro Oocyte Maturation and Embryo Development and Expression of EGF mRNA in Bovine Oocytes and Embryo I. Influence of Cumulus Expression and Maturation and Embryo Development during Bovine Oocyte Maturation In vitro by Addition EGF

  • Kim, Kwang-Sig;Kim, Chang-Keun;Chung, Yung-Chai;Hwang, Seong-Soo;Park, Jin-Ki;Chang, Won-Kyong
    • Proceedings of the KSAR Conference
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    • pp.28-28
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    • 2001
  • The objective of this study was to the effect on subsequent development of EGF present in defined medium during bovine 1)oocyte maturation or 2)embryo culture. The presence of EGF during IVM, irrespective of concentration(1, 10, 100ng/$m\ell$), stimulated cumulus expansion and significantly increased the proportion of oocytes attaining metaphaseII, the rate of cleavage, and develop to blastocyst. 1. In the group of EGF-added medium(1, 10, 100ng/$m\ell$), nuclear maturation rate for in vitro maturation was 91% to 92% but was not significantly higher than control group(87%). 2. For in vitro maturation, in the group of EGF-added medium(1, 10, 100ng/$m\ell$)the rate of cumulus cell expansion degree 2 ranged from 81% to 87%, which was significantly higher than the control group(medium with EGF not added). The rate of in vitro fertilization, developing to 2-to 4- cell stage, was 76% to 80%, which was also significantly higher(p<0.05)than control group(62%). 3. For in vitro maturation, in the group of EGF added in medium(1, 10, 100ng/$m\ell$)the development rate to blastocyst was 24.3% to 27%, which was significantly higher than control group(13.7%). The total cleavage rate in the group of EGF-added medium was 77% to 82%, which was higher than control group. 4. The development rate to blastocyst for 6 days of cultivation and the hatching blastocyst were 30.6% and 59.1%, respectively, in the group of 100ng/$m\ell$ of EGF, which were significantly higher(p<0.05)than control group(14.0% and 24%, respectively), The numbers of cells in blastocyst were 140.2 and 148, respectively, in 10ng/$m\ell$ and 100ng/$m\ell$ of EGF-added medium, which were higher than 108.5 in control group. 5. The development rate of in vitro fertilized embryos to blastocyst in 10ng/$m\ell$ of EGF-added medium co-cultured with somatic cell was 28%, which was significantly higher(p<0.05)than control group(11.8%). The numbers of cells in blastocyst were 141.6 for EGF-added medium and 145 for EGF+co-culture group, which were higher than control(101.6)and medium co-cultured with somatic cells(110.6). These results showed that in vitro maturation and fertilization, EGF was found a significant effect of increase of development rate to blastocyst and cell number.

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Small and Medium Venture Companies' Exterior Information Network Heterogeneity and New Technology Development Capability: The Mediating Role of Product Planning Capability (중소 벤처기업의 외부 정보 네트워크의 다양성과 신기술 개발 역량: 제품 기획 역량의 매개적 역할)

  • Hau, Yong Sauk
    • Journal of Digital Convergence
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    • v.16 no.3
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    • pp.121-127
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    • 2018
  • Enterprises' new technology development capability is essential for creating successful product innovation and process innovation in the heavy competition in the business context changing quickly with technology convergence and development. Based on the open innovation perspective, this research develops a research model about the direct effect of small and medium venture companies' exterior information network heterogeneity on their new technology development capability and the mediating impact of their product planning capability on this direct effect. This study statistically tests the research model by using the ordinary least squares regression with the 683 small and medium venture companies, providing two major findings as follows. One finding is that small and medium venture companies' exterior information network heterogeneity positively impacts their new technology development capability. The other finding is that small and medium venture companies' product planning capability perfectly mediates the positive effect of their exterior information network heterogeneity on their new technology development capability.

Effects of Medium Components and Growth Regulators on Callus Development and Shoot Regeneration from Shoot Explants of Black Locust (Robinia pseudoacacia)

  • Shin, Dongill;Han, Kyung-Hwan;Sul, Ill-Whan
    • Journal of Life Science
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    • v.9 no.1
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    • pp.50-53
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    • 1999
  • Effects of growth regulators and medium components were tested for shoot multiplication and callus growth from shoot explants of black locust. During shoot multiplication, callus growth at the cut end of shoot explants proceeded shoot development. The basal callus growth seemed to be a function of both mineral components and cytokinin supplemented in the medium. Maximum callus growth was induced by 0.5${\mu}$M BAP and the callus growth decreased as the level of BAP increased. Positive correlations were found between basal callus growth, and shoot multiplication and growth. Shoot multiplication was greatest on BSM medium (black locust shoot culture medium) supplemented with 1 $\mu$M BAP. With medium containing high nitrogen content, both shoot multiplication and growth were significantly enhanced. A new BRM medium was the most effective for rooting of black locust among three rooting media tested.

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Development of Loading Machine of Culture Medium for Oyster Mushroom Production - Investigation of Physical Properties and Element Design of System - (느타리버섯 재배용 배지 입상 장치 개발(1) - 배지 물성 조사 및 장치요소 설계 -)

  • Lee, Kyung-Jin;Lim, Hak-Kyu;Kim, Tae-Han
    • Journal of Biosystems Engineering
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    • v.34 no.4
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    • pp.211-219
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    • 2009
  • In the process of oyster mushroom production, loading work of culture medium needs the most intensive labor power and cost. Therefore, the development of culture medium loading machine causes to reduce the manpower and cost. The main objectives of this study are identify cultivating environment, physical properties of culture medium and to make an element design of culture medium loading machine. The results are summarized as follows: 1. The moisture content and density of popularly used culture medium were 70%(w.b), $26\;kg/m^3$, respectively. 2. Pressure of the blower increased as the impeller speed increased, and the opening ratio of pressure controller decreased. 3. Recommendable c1earance(${\delta}$) between an impeller plate and a blower case was 25 mm at an impeller speed of 3183 rpm 4. Discharge device of type B with a hopper and suit was better than type A with a hopper.

Methods of temperature measurement of medium in bottle during sterilization (병재배 배지살균 시 배지내부의 실시간 온도측정 방법)

  • Cheong, Jong-Chun;Jhune, Chang-Sung;Lee, Chan-Jung;Oh, Jin-A;Shin, Pyeong-Gyun
    • Journal of Mushroom
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    • v.9 no.2
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    • pp.74-79
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    • 2011
  • This study was carried out to develop a method related to measuring the temperature of sterile medium in bottle cultivation. When the medium is sterilized, the device is able to be inserted inside of the medium and the temperature can be directly measured in real time although high temperature and pressure are detected in the sterilization. This device can be able to measure the sterilization temperature in short intervals inside of autoclave and medium used in bottle cultivation. As the method were applied to the field cultivation of mushroom, we could produce mushroom in consistent manner through the optimum sterilization of the medium.

Development of Cabbage Juice Medium for Industrial Production of Leuconostoc mesenteroides Starter

  • Jeong, Eun Ji;Moon, Dae Won;Oh, Joon Suk;Moon, Jin Seok;Seong, Hyunbin;Kim, Kwang Yup;Han, Nam Soo
    • Journal of Microbiology and Biotechnology
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    • v.27 no.12
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    • pp.2112-2118
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    • 2017
  • Leuconostoc mesenteroides is used as a starter to produce high-quality kimchi products. In this study, an efficient and economical cabbage juice medium (CJM) was developed by process optimization of cabbage extraction and pasteurization and by compositional supplementation of various lacking nutrients. The pasteurized cabbage juice was determined to be a good medium candidate to cultivate L. mesenteroides, showing maximal cell numbers ($9.85{\times}10^8CFU/ml$) after 24 h. Addition of sucrose and yeast extract with soy peptone resulted in increment of bacterial cell counts in CJM, showing the supplementing effect of the lacking nutrients. Furthermore, addition of shell powder gave a protective effect on bacterial cells by preventing pH decline and organic acid accumulation in CJM, resulting in a 2-fold increase of bacterial counts. The optimized composition of CJM was 70% cabbage juice diluted with water, 0.5% (w/v) sucrose, 1% (w/v) yeast extract, 1% (w/v) soy peptone, and 1.5% (w/v) ark shell powder. The CJM developed in this study was able to yield a comparable level of bacterial counts with MRS medium and reduced the cost by almost 10-fold.