• Title, Summary, Keyword: Medium development

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Development of a Selective Medium for the Fungal Pathogen Fusarium graminearum Using Toxoflavin Produced by the Bacterial Pathogen Burkholderia glumae

  • Jung, Boknam;Lee, Sehee;Ha, Jiran;Park, Jong-Chul;Han, Sung-Sook;Hwang, Ingyu;Lee, Yin-Won;Lee, Jungkwan
    • The Plant Pathology Journal
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    • v.29 no.4
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    • pp.446-450
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    • 2013
  • The ascomycete fungus Fusarium graminearum is a major causal agent for Fusarium head blight in cereals and produces mycotoxins such as trichothecenes and zearalenone. Isolation of the fungal strains from air or cereals can be hampered by various other airborne fungal pathogens and saprophytic fungi. In this study, we developed a selective medium specific to F. graminearum using toxoflavin produced by the bacterial pathogen Burkholderia glumae. F. graminearum was resistant to toxoflavin, while other fungi were sensitive to this toxin. Supplementing toxoflavin into medium enhanced the isolation of F. graminearum from rice grains by suppressing the growth of saprophytic fungal species. In addition, a medium with or without toxoflavin exposed to wheat fields for 1 h had 84% or 25%, respectively, of colonies identified as F. graminearum. This selection medium provides an efficient tool for isolating F. graminearum, and can be adopted by research groups working on genetics and disease forecasting.

Comprehensive model formedium composition for mushroom bottle cultivation (버섯 병재배를 위한 배지조성물의 종합관리 모델)

  • Cheong, Jong-Chun;Lee, Chan-Jung;Moon, Ji-Won
    • Journal of Mushroom
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    • v.14 no.3
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    • pp.111-118
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    • 2016
  • The medium used for bottle cultivation of mushrooms is made according to a specific workflow; this includes mixing the raw materials, adjusting the moisture content, placing the mixture into bottles, and sterilizing and cooling the medium. Medium composition should be kept constant throughout the year. placed in the bottle, choice of materials, moisture content, weight of the medium, bulk density, nitrogen content, three-phase medium, such as suitable conditions. To achieve constant medium composition a comprehensive model has been established in accordance with the technology developed in the work process with respect to medium compositions piecemeal. By comprehensively applying the various methods to establish medium composition, our country can expect to grow at over four seasons and overcome the many difficulties in mushroom mycelial growth periods, cultivation periods, quantity, and quality.

Recycling Post-harvest Medium from Bottle Cultivation for Oyster mushroom(Pleurotus ostreatus) (버섯 병재배 수확후배지의 느타리버섯 배지에 알맞은 재활용 수준)

  • Cheong, Jong-Chun;Lee, Chan-Jung;Shin, Pyung-Gyun;Suh, Jang-Sun
    • Journal of Mushroom
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    • v.10 no.4
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    • pp.167-173
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    • 2012
  • This study was carried out to test the recycling post-harvest medium from other mushroom bottle cultivation as a secondary medium of the oyster mushroom. In the post-harvest medium from winter mushroom and king oyster mushroom cultivation, oyster mushroom varieties in Chunchu-2ho and Manchuri fruit bodies yields compared with control group tend to be low. After recycling the post-harvest medium, it was replaced by basal medium up to 50%, of which the fruit bodies with stable yield increase from 10% to 30% were increased.

Effect of the Culture Media of Bovine Oviductal Epithelium on Development of the Early Bovine Embryos Derived from in vitro Fertilization (소 난관상피세포배양액이 체외수정 유래 분할란의 발육에 미치는 영향)

  • Park Jong-Im;Hwang Woo-Suk;Jo Choong-Ho;Lee Byeong-Chun
    • Journal of Veterinary Clinics
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    • v.9 no.1
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    • pp.323-332
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    • 1992
  • The present study was carried out to examine the effect of oviduct epithelium and its conditioned medium on e development of early bovine embryos in vitro. Oocytes obtained from ovarian follicles of slaughtered cows were cultured in TCM199 with 10% fetal calf serum for 22-24hrs and then fertillzed in vitro using frozen-thawed semen treated with BO-caffein, BO-BSA(20mM heparin added). Oviduct epithelium was collected in each stage of the estrus cycle and conditioned medium was the medium in which oviduct epithelium in early luteal stage was cultured. In vitro fertilized bovine embryos of 1~2 cell were co-cultured with oviduct epithelium from different estrus cycles, cultured in conditioned medium, and cultured in rabbit oviduct. The cleavage rates of in vitro fertilized early bovine embryos co-cultured with oviduct epithelial cell from early luteal, luteal and follicular phase of estrus cycle(67.2~70.8%) and cultured in conditioned medium(56.7%) were significantly(p<0.05) higher than that of the control(44.2%) The rate of development to morula or blastocyst stage in oviduct epithelial cell co-culture(15.3~32.5%) from three phase of estrus cycles and conditioned medium(14.5%) were significantly(p<0.05) higher than that of the control(5.2%). The oviduct epithelial cell from early luteal phase gave a significantly( p<0.05) higher rate of development to morula or blastocyst stage than both luteal and follicular phase. The results of in vivo culture in rabbit oviduct of early bovine embryos were 52.1% for the cleavage rate and 26.7% for the rate of development to morula or blastocyst stage.

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Gametophyte Propagation and Sporophyte Formation of Asian Chain Fern [Woodwardia japonica (L. f.) Sm.] Under Various Medium Conditions In Vitro and Ex Vitro

  • Cho, Ju Sung;Jang, Bo Kook;Park, Kyungtae;Lee, Ha Min;Lee, Cheol Hee
    • Korean Journal of Plant Resources
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    • v.32 no.6
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    • pp.735-742
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    • 2019
  • This study investigated a suitable method that could be applied for Asian chain fern [Woodwardia japonica (L. f.) Sm.] to propagate gametophytes and promote sporophyte formation. The gametophytes used in all experiments were obtained from germinated spores in vitro and were subcultured at 8-week intervals. The most appropriate media for gametophyte propagation was identified by culturing 300 mg of gametophyte in Murashige and Skoog (MS) basal medium (1/8, 1/4, 1/2, 1, 2), and Knop medium for 8 weeks. As a result, fresh weight of the gametophyte was increased by 56.7-fold on MS medium. Moreover, antheridium formation as well as gametophyte growth was improved on MS medium, especially. To improve the sporophyte formation ex vitro, 1.0 g of gametophyte was ground with distilled water and spread on eight combinations onto four different culture mediums, such as bed soil, peat moss, perlite and decomposed granite. Then generation and growth of sporophytes were investigated after cultivation for 10 weeks. As a result of this experiment, peat moss had a promotive effect of sporophyte formation at single-use and mixed culture soils. In particular, a mixture of bed soil, peat moss and perlite in a 1:1:1 ratio (v/v/v) led to the accelerated formation (782.5 ea/pot) and the frond growth of sporophytes. This included increases in length and width of fronds. However, promotive effect of gametophyte growth and sporophyte formation was not found at single-use and treatment with high ratio of bed soil.

A study on the model of regional balanced development for promoting small and medium sized enterprise (중소기업육성을 위한 지역균형발전 모형 연구)

  • Chang, Seog-Ju;Park, Roh-Gook;Yoon, Byung-Seop
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.2 no.1
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    • pp.133-151
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    • 2007
  • In the era of 21st new century. reinforcement of regional competitiveness by establishing innovation system is taking its place of main small and medium sized enterprise policies in major developed countries. The model of regional balanced development for promoting small and medium sized enterprise is a main frame of governmental small and medium sized enterprise policies. which complements national innovation process. And in the level of local area, the model is regional network system, molded in the process of induction, application, modification, proliferation of new technologies and knowledge by local businessman and innovators. This study focused on the strategy development for small and medium sized businesses to increase local competitive power, in terms of regional balanced development. In order to find the real problems and improvement alternatives for local competitive power, this study both reviewed the common strategies and action programs advanced in developed countries for small businesses, and did research on the current economic conditions in local area. Based on the lessons from foreign cases and the analysis of the current situations in small business in local area, the study suggested the model of regional balanced development for promoting small and medium sized enterprise including formation of innovation network system through the invitation of leading firms in the area, industry-university partnerships, and development and attraction for small and medium sized businesses.

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The development of internet based ship design support system for small and medium sized shipyards

  • Shin, Sung-Chul;Lee, Soon-Sup;Kang, Dong-Hoon;Lee, Kyung-Ho
    • International Journal of Naval Architecture and Ocean Engineering
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    • v.4 no.1
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    • pp.33-43
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    • 2012
  • In this paper, a prototype of ship basic planning system is implemented for the small and medium sized shipyards based on the internet technology and concurrent engineering concept. The system is designed from the user requirements. Consequently, standardized development environment and tools are selected. These tools are used for the system development to define and evaluate core application technologies. The system will contribute to increasing competitiveness of small and medium sized shipyards in the 21st century industrial en-vironment.

Effect of Insulin, Transferrin and Platelet-Derived Growth Factor Supplemented to Synthetic Oviduct Fluid Medium on In Vitro Development of Bovine Embryos Matured and Fertilized In Vitro (합성난관배양액에 첨가된 Insulin, Transferrin 및 Platelet-Derived Growth Factor (PDGF)가 소 수정란의 체외발육에 미치는 영향)

  • 이은송
    • Journal of Embryo Transfer
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    • v.12 no.3
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    • pp.283-291
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    • 1997
  • In vitro development of bovine embryos is affected by many factors such as energy substrates, amino acids, and some growth factors. It has been reported that mRNA of insulin, PDGF and their receptors are detected in cow embryos, and that some chelating agents such as EDTA and transferrin have beneficial role on mouse and bovine embryos. The author hypothesized that insulin, transferrin arid PDGF added to a culture medium increase in vitro development of bovine embryos by chelating toxic substance(s) or increasing cell growth and metabolism. Immature oocytes from slaughtered ovaries of Holstein cows and heifers were matured for 24 hours in a TCM199 containing 10% fetal calf serum, FSH, LH and estradiol with granulosa cells in vitro. Matured oocytes were coincubated with sperm for 30 hours in a modified Tyrode's medium (IVF). Embryos cleaved to 2- to 4-cell at 30 hours after IVF were selected and cultured in a 30-$\mu$l drop of a synthetic oviduct fluid medium (SOFM) containing 0.8% BSA, Minimum Essential Medium essential and non-essential amino acids, and insulin, transferrin or PDGF for 9 days. Supplementation of a SOFM with insulin, and /or transferrin did not increase develop-mental rate to expanding and hatching blastocyst of 2- to 4-cell bovine embryos compared with control. The highest developmental rate to hatching blastocyst was shown when PDGF was added at the concentration of 10 ng /ml among the supplementing doses tested in the present study (p<0.05). Addition of PDGF without insulin to a SOFM could not increase embrye development, but combined addition of PDGF with insulin significantly increased (p<0.05) embryo development to hatching blastocyst (50%) compared with control (38%). In conclusion, insulin and PDGF supplemented to a SOFM may act synergistically and have beneficial effect on in vitro development of 2- to 4-cell bovine embryos matured and fertilized in vitro.

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Effect of Oocyte Maturation Medium, Cytochalasin Treatment and Electric Activation on Embryonic Development after Intracytoplasmic Sperm Injection in Pigs

  • Lee, Joohyeong;Choi, Jung Hoon;Lee, Seung Tae;Hyun, Sang-Hwan;Lee, Eunsong
    • Journal of Embryo Transfer
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    • v.28 no.2
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    • pp.127-132
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    • 2013
  • The objective of this study was to examine the effect of in vitro maturation (IVM) medium, cytochalasin B (CB) treatment during intracytoplasmic sperm injection (ICSI), and electric activation on in vitro development ICSI-derived embryos in pigs. Immature pig oocytes were matured in vitro in medium 199 (M199) or porcine zygote medium (PZM)-3 that were supplemented with porcine follicular fluid, cysteine, pyruvate, EGF, insulin, and hormones for the first 22 h and then further cultured in hormone-free medium for an additional 21~22 h. ICSI embryos were produced by injecting single sperm directly into the cytoplasm of IVM oocytes. The oocytes matured in PZM-3 with 61.6 mM NaCl (low-NaCl PZM-3) tended to decrease (0.05

Promotion of in vitro shoot proliferation in rose by addition of liquid medium to culture (액체배지 첨가에 의한 장미 기내 신초 증식 촉진)

  • Lee, Ye Ji;Lee, Jung Lim;Hyung, Nam-In;Kim, Seung Tae;Lee, Eun Kyung;Kwon, O Hyeon;Kim, Won Hee;Lee, Su Young
    • Journal of Plant Biotechnology
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    • v.39 no.4
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    • pp.305-308
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    • 2012
  • To promote the growth and proliferation of in vitro rose (Rosa hybrida L) shoots, a liquid medium was added to shoot culture. Shoots were obtained by culturing internodes of four cultivars, 'Antique Curl', 'Shiny Orange', 'White Zen', and 'Red Zen', and then were proliferated by the subculture two times. An addition with 10~15 mL of liquid medium enhanced the shoot elongation of all four cultivars. However, the effect of liquid medium addition to culture of in vitro shoot for proliferation was dependent on cultivars of rose.