• Title, Summary, Keyword: Medium development

Search Result 3,632, Processing Time 0.063 seconds

Making yeast culture medium with rapeseed pollen granules (유채 꽃가루 분말을 이용한 효모 배양배지 제조)

  • Lee, Yong-Hwa;Kim, Kwang-Soo;Jang, Young-Seok;Choi, In-Hu;Lee, Kyeong-Bo
    • Journal of the Korean Applied Science and Technology
    • /
    • v.32 no.1
    • /
    • pp.93-99
    • /
    • 2015
  • The aim of this study was to analyze nutritional compositions of rapeseed pollen granules and to determine the possible usage of pollen granules as a yeast culture medium. Rapeseed pollen granules (per 100 g) were consisted of carbohydrate 58.9 g, protein 20.8 g, fat 4.1 g, ash 2.5 g and water 13.7 g. And fructose (13.7 g), glucose (11.1 g), and sucrose (6.6 g) of sugars and K (606.7 mg) and P (603.3 mg) of minerals were highly contained. In addition, free amino acids such as glutamic acid (2,482.4 mg), aspartic acid (2,136.5 mg), lysine (1,648.3 mg), and leucine (1,631.1 mg) were present at a higher level. When liquid medium, which was made from cracked pollen granules (5, 10, 15, 20, 25, 30, and 40 g/L), was tested for yeast culture, liquid medium containing pollen granules over 15 g/L showed higher yeast growth than YPD medium (control). Liquid medium containing both cracked pollen granules (15 g/L) and NaCl (1 ~ 20 g/L) improved yeast growth than the liquid medium without NaCl. In addition, when yeast growth was tested on solid medium made from pollen granules (15 g/L) at $30^{\circ}C$ for 2 days, yeast colonies were equally well-formed like those grown on YPD medium. Overall, rapeseed pollen granules have potential properties on yeast growth and could be used as a primary source for yeast culture.

A Study on the Korea Distribution Promotion Policy and Adjustment Policy (국내 유통진흥정책과 유통조정정책에 대한 고찰)

  • Kim, Dae-Yun;Kwon, Sung-Ku
    • The Journal of Distribution Science
    • /
    • v.11 no.4
    • /
    • pp.89-97
    • /
    • 2013
  • Purpose - The purpose of this study is to systematically review the background of the Korean distribution promotion policy and distribution adjustment policies along with related regulations and policies. Research design, data, and methodology - Domestic distribution policy and relevant laws were examined through a review of existing research literature. The results of the development process of the domestic distribution policy, promotion policies, and adjustment policies are summarized below. Results - The results are summarized as follows. First, the purpose of the development of the domestic distribution promotion policy was to strengthen the competitiveness of the small and medium business industry through structural advancement of the small and medium industry. By expanding the managerial base for the small and medium industry, a new balance could be created in the national economy. There was a requirement for an early assistance policy for small and medium businesses as a base of these businesses in the distribution industry developed from their original model of catering to a traditional market of retail shops. Since 1996, there was a need for this early assistance policy due to the expansion and rapid growth of large scale stores causing a change in the consumption pattern for distribution markets and the decline of large enterprises. Second, the government supports small and medium business distribution through distribution promotion policies by supporting an organization promoting small business and supporting innovation in the distribution system. Third, in 1961 a business mediation system was established to protect small and medium industries. The Small and Medium Business Administration advises conglomerates to postpone acquisitions, restrain expansion of the business, or to reduce business scale if small businesses undergo an adverse effect such as decreasing demand because large companies are expanding into their areas. Fourth, the Distribution Adjustment Policy managed large-scale store regulation as follows: ① limitation on construction by urban planning ordinance, ② limitation on location based on traffic impact assessments, ③ regulation based on business guidelines by chiefs of autonomous bodies, ④ regulation on mandatory holidays and limitation of business hours. This large-scale store regulation is a policy introduced by authority to increase competitiveness of small and medium business distribution by the government. Conclusions - As discussed in this study, the distribution promotion policy and distribution adjustment policy are government distribution policies focused on the protection of the small and medium distribution businesses. This study is timely, since it was planned when the strengthening of the revisions of the Distribution Industry Development Act, aimed to protect small and medium retailers and merchants, was under discussion. The significance of this study is that it offers insights for the development of new policies in the future and an opportunity to consider the background of the distribution policy by the government.

  • PDF

City Shrinking Simulation followed by a Decrease of Population Trend in Small and Medium-Sized Local Cities (지방 중소도시의 인구감소추세에 따른 도시 축소 시뮬레이션)

  • Lee, Da-Geon;Yoon, Cheol-Jae
    • Journal of the Architectural Institute of Korea Planning & Design
    • /
    • v.35 no.4
    • /
    • pp.127-134
    • /
    • 2019
  • Recently, the city development policies are being switched to the policies focusing on recession worldwide. Even though South Korea is also facing the phenomena that small and medium-sized cities are downsized because of a decrease in population and an aging society problem, there is lack of solutions and even they are still planning city development policies based on prospect of high growth. This study is featuring 4 viewpoints depending on shrinking population trend in 7 small and medium sized local cities, which are chosen as downsized cities. It is dealing with population peak, current population, 2030 population, and 2040 population viewpoints. And this study uses downsizing simulation that suggests optimum area that fits population and then shows visual check of the status of the small and medium sized local cities. The area of the small and medium sized local cities are expanded more than needs when they are on population peak point. However, they need only 20% to 25% of current area in 2030 and 2040. The most important thing is realizing the seriousness and facing up to the 'shrinking' phenomena that each small and medium-sized cities suffer. Therefore, this study has significance for presenting the status of small and medium-sized cities' problems in South Korea.

Ultrarapid Freezing of Mouse 2-Cell Embryos (생쥐 2-세포기 수정란의 초급속동결)

  • 강만종;이철상;한용만;유대열;이경광
    • Korean Journal of Animal Reproduction
    • /
    • v.14 no.1
    • /
    • pp.9-16
    • /
    • 1990
  • This study was carried out in order to investigate effects of cryoprotectant concentration and equilibration time on survival of ultrarapidly frozen 2-cell mouse embryos. Mouse 2-cell embryos, following dehydration by exposure to DMSO and sucrose, were directly immersed into liquid nitrogen and thawed in 37$^{\circ}C$ water. Viability was defined by development rate to the blastocyst stage after in vitro culture for 72 hours. The results are summarized as follows ; 1. When 0.25M of sucrose was added into the freezing medium at various concentrations of DMSO and dilution medium, higher development rate of embryo was obtained in 3.0M DMSO concentrations (82.6%). However, when sucrose concentraitons of 0.25 and 0.5 M were added to the freezing medium with 3.0 M DMSO and dilution medium, development rate of embryos were 81.7% and 24.1%, respectively. 2. In the equilibration time at room temperature, higher development rate was attained after short period of time (2.5min) in 3.0 M DMSO+0.25 M sucrose (85.9%). 3. The development rate of embryos at in vitro 2-cell, in vitro 2-cell, solution control and untreated control was 84.6%, 90.9%, 89.9%,, and 89.7%, respectively.

  • PDF

Development of hull form of small and medium size coastal ships (연안항로용 중소형 섭박의 선형 개발)

  • 조용진;고창두;김성환
    • Journal of Ocean Engineering and Technology
    • /
    • v.11 no.2
    • /
    • pp.131-137
    • /
    • 1997
  • The technology of small and medium sized shipbuilding companies is lower relatively to major shipbuilding companies, and should be up-graded from the viewpoint of national economic development. In spite that hul form design is one of the most important elements with respect to shipbuilding technology, small and medium sized shipbuilding companies are incapable of developing hull form. Inorder to support technically such companies hull form development was carried out for G/T 999ton class tanker and D/W 4990ton class tanker which are the typical vessels operating in coastal area. The operating efficiency for the developed hull forms are impeoved by 30% for G/T 999ton class tanker and 24% for D/W 4990 tonclass tanker respectively. The results of hull form development including ship model test and performance analysis are presented in this paper.

  • PDF

A Study on the Development Tendency of Housing Development District in Local Small and Medium Cities - Focused on the Completed District from 1992 to 1996 - (지방중소도시 택지개발지구 단독주택지의 개발경향에 관한 연구 - 1992년부터 1996년까지 조성된 지구를 중심으로 -)

  • Choi, Ki-Young
    • Journal of the Korean housing association
    • /
    • v.22 no.2
    • /
    • pp.63-71
    • /
    • 2011
  • The growth of housing complex in the Housing Development District in local small and medium cities can be defined to process individual lots are possessed by buildings. The study is the basic research of analysis for the internal factors that affect on the each lot, and is for hypothesis of the verification about the Housing Development District growth. So, it can be applied in the Housing Development District of the site conditions and restriction of use of buildings and infrastructure works and the constant boundary of the region. The rate of the development is the occupation of the individual lots per entire lots was limited as the factor of analysis. As above conditions haver no possibility to change in the Housing Development District, the results of this study and further are available to consider. By the results of the connected study, it can be used for Planning and management of the Housing Development District in local small and medium cities. by the adjustment of the relation between growth tendecy and city's properties or district characters.

Effects of Growth Factors and Hexoses on In Vitro Development in Porcine Embryos (돼지수정란의 체외발육에 있어서 Growth Factors와 Hexoses의 영향)

  • 윤선영;이상영;정희태;양부근;김정익;박춘근
    • Korean Journal of Animal Reproduction
    • /
    • v.27 no.3
    • /
    • pp.249-258
    • /
    • 2003
  • The aim of this study was to investigate the effects of different hexoses in medium with IGF-I and/or EGF on in vitro development of porcine embryos. In first experiment, when the embryos were cultured in medium with concentrations of 5 ng/ml IGF-I and 10 ng/ml EGF, the morula and blastocyst rates were higher than in another culture conditions (P<0.05). In second experiment, the effect of IGF-I in medium with different hexoses during the embryo culture was examined. The higher cleavage rates were obtained in medium with glucose and IGF-I (P<0.05). However, the higher proportion of embryos developed to morula and blastocyst stages in medium with IGF-I were obtained than in medium without IGF-I in medium with glucose, mannose and galactose. In third experiment, the effect of EGF on in vitro development of porcine embryos in medium with different hexoses were examined. In the culture medium was supplemented with glucose, the higher proportions (P<0.05) of embryos developed to molura and blastocyst stages were obtained in medium with that than without EGF. However, the proportions of embryos developed to blastocyst stage were not significantly different between with and without of EGF in medium with different hexoses. In firth experiment, the effects of presence of IGF-I and EGF in medium with different hexoses on in vitro development of porcine embryos were examined. When culture medium was supplemented with IGF-I and EGF, the higher proportions of oocytes cleaved and developed to 8-cell stage were obtained in medium with glucose than mannose, galactose and fructose (P<0.05). These results show that glucose and growth factors, supported the development in vitro of porcine embryos, especially with greater embryo cleavage and development in medium with glucose added to media with IGF-I and/or EGF.

Effect of Supplements Added into the Maturation Medium on Lipid Droplets Formation and In Vitro Development of Immature Porcine Oocytes.

  • Park, In-Kyoung;Song, Hai-Bum
    • Proceedings of the KSAR Conference
    • /
    • /
    • pp.242-242
    • /
    • 2004
  • This study was conducted to investigate the effects of various supplements added into maturation medium of immature porcine oocytes on quantity of cytoplasmic lipid droplets(LD), subsequent fertilization and development to the blastocyst stage in vitro. The basic maturation medium was TCM 199 + 1 ㎍/㎖ FSH, 0.57 mM cystein, 10 ng/㎖ EGF and was supplemented various supplements(10% FBS, 10% pFF, 0.4% BSA, 1.0% BSA, 0.4% PVP, 1.0% PVP). (omitted)

  • PDF

Mechanism for the Action of Co-culture (공배양의 작용기전에 관한 연구)

  • Kim, Mi-Kyoung;Joo, Bo-Sun;Kim, Mi-Sun;Moon, Hwa-Sook;Lee, Kyu-Sup;Kim, Han-Do
    • Clinical and Experimental Reproductive Medicine
    • /
    • v.27 no.1
    • /
    • pp.39-46
    • /
    • 2000
  • Objective: A number of studies to improve in vitro culture conditions have been tried over past ten years by using co-culture system with helper somatic cells. However, the mechanism of coculture is poorly understood. This study was designed to understand the mechanism for the mode of actual action of co-culture using co-culture system of ICR strain's 1-cell embryos with human oviduct epithelial cells by examining the effect of conditioned medium and contactless coculture using a cell culture insert on the embryo development and by measuring the level of superoxide anion from conditioned medium after co-culture. Methods: ICR strain's zygote embryos were cultured in medium alone (control), coculture, conditioned medium, or contactless coculture system for 6 days. Conditioned media (CM) were prepared as following 5 groups. All CM were collected after culturing oviduct cells for 2 days. CM-1 was stored at $-20^{\circ}C$ until use, and CM-2 was prepared just before use as a culture medium. CM-3 was cocultured with embryos and retrieved just before use. CM-4 and CM-5 were derives from the microfilteration of CM-2 and CM-3, respectively, using Microcon-10 (10 kDa molecular weight cut-off). The percentage of the embryos developed to hatched blastocyst stage and the level of superoxide anion in supernatant from medium alone culture (control), coculture, and contactless coculture were measured. Results: The rates of embryo development to the hatched blastocyst stage were significantly higher in coculture (43%) than in control (0%) (p<0.05). The CM-1 group had no embryo development since 2-cell embryonic stage, whereas the CM-2, CM-3, CM-4 and CM-5 groups had the improved development to 4 or 8 cell embryo stage, but the similar rate of development to hatched blastocyst compared to control. The effect of coculture on embryo development was disappeared in the contactless coculture group. The level of superoxide anion was significantly reduced in coculture group compared to control. Conclusion: It is concluded that the present coculture system overcomes the 2-cell block in vitro and improves the embryo development. This beneficial effect may be due to the direct cell-cell contact between embryo and helper cells or the removal of deleterious components from medium rather than the embryotrophic factors.

  • PDF