• Title, Summary, Keyword: Medium development

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Composition of Culture Medium and Culture Conditions for In vitro Culture of Rice Panicle

  • Lee, Jin-Ho;Lee, Ho-Jin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.1
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    • pp.32-37
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    • 2000
  • The in vitro culture of rice panicles is a culturing technique only panicle without other organs in culture solution containing organic substance, so that would be useful to study how assimilate supply affects grain development and maturation. To find the optimum stage for in vitro culture, rice panicles grown in greenhouse were sampled periodically after anthesis and cultured in nutrient medium. The panicles older than 1 weeks after anthesis had produced normal grains. Grain-filling was apparently dependent upon sucrose concentration (8-12 %) in medium, but not affected by nitrogen concentration supplied with glutamine. As far as rice panicle was supplied with sucrose and N in nutrient medium, grains continued accumulation of dry matter and maturation regardless to light condition. Considerably, grain-filling was improved when panicles were positioned horizontally inside flask, so that each grain was partially submerged to nutrient medium.

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Purification of Embryogenesis Stimulating Activity from Bovine Oviduct Epithelial Cell Conditioned Medium and Its Effect on the Development of Bovine Embryos (소 난관상피세포 배양액 유래 배발육촉진인자의 정제와 소 수정란 체외배양시 발육에 미치는 영향)

  • 김현일;노상호;박종임;신태영;이병천;황우석
    • Journal of Embryo Transfer
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    • v.13 no.1
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    • pp.43-52
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    • 1998
  • The embryogenesis stimulating activity(ESA) had been shown in co-culture of embryos with bovine oviduct epithelial cell(BOEC) and culture in BOEG-conditioned medium. The present study was undertaken to purify and quantify the embryotropic proteins and to determine the optimum concentration of the embryotropic protein for the proper development of embryos. In BOEC-conditioned medium, five major bands of proteins were detected(66, 53, 40, 32 and 24 kDa) by SDS-PAGE. From these proteins, 288pg of protein that had a 32kDa molecular weight was purified by gel filtration column and perfusion chromatography ion-exchange column. When purified protein was supplemented to the in vitro culture media at various concentrations in protein-free media, 2.5$\mu$g /ml supplement group showed significantly higher rates of embryo development into morula /blastocyst stages than other groups(p<0.05). In conclusion, we purified 32kDa protein from BOEC-conditioned medium and this protein showed optimum embryogenesis stimulating effect at 2.5$\mu$g /ml.

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Effects of Glucose, Lactate and Pyruvate on Development of In Vitro Matured and Fertilized Porcine Embryos (Glucose, Lactate 및 Pyruvate가 돼지 체외수정란의 초기발생능에 미치는 영향)

  • 오건봉;박병권;서길웅;이규승
    • Korean Journal of Animal Reproduction
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    • v.19 no.1
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    • pp.9-14
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    • 1995
  • This study was conducted to investigate the effect of energy source on development of in vitro development of in vitro matured and fertilized porcine 2-cell embryos. The relative preferences of glucose, lactate and pyruvate for in vitro development of porcine 2-cell embryos were determined. The results obtained are as follows. 1. 33.3, 20.8 and 29.2% of porcine embryos reached morula stage in addition to lactate, glucose, and both glucose and lactate in the culture medium as energy source, respectively. 2. 38.5, 15.4 and 26.9% of porcine embryos reached morula stage in addition to pyruvate, glucose, and both glucose and pyruvate in culture medium as energy source, respectively. 3. 42.9, 21.4 and 28.6% of porcine embryos reached morula stage in addition to pyruvate and lactate, glucse alone, and glucose, lactate and pyruvate in culture medium as energy source, respectively.

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A Plan to Strengthen the Competitveness of Small and Medium Manufacturing Enterprises in Korea -Focusing on the Support for Technological Development (우리나라 중소기업의 기술경쟁력 강화방안 - 기술지원중심으로)

  • Chung, Won-Kee;Hong, Keun
    • Journal of the Society of Korea Industrial and Systems Engineering
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    • v.18 no.35
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    • pp.95-106
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    • 1995
  • This study is aimed at development a plan to strengthen the competitiveness in technology of small and medium manufacturing enterprises, which can be assumed mainly by small and medium manufacturing companies, to improve the competitiveness of domestic industry. To remove these obstacles and strengthen the technological competitiveness, internally it is required to change the attitude of management about the development and innovation of technology first. Promotion of favorable atmosphere for the technological development and organized activity to acquire technological information are additional requirements. In conclusion, to survive the competition and to secure national industrial competitiveness, each individual company needs to endeavor for technological innovation and improvement in product quality and productivity.

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In vitro multiple shoot proliferation and plant regeneration in rose(Rosa hybrida L.)

  • Lee, Su-Young;Jung, Ji-Hye;Kim, Jeong-Hee;Han, Bong-Hee
    • Journal of Plant Biotechnology
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    • v.35 no.3
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    • pp.223-228
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    • 2008
  • This study was conducted to investigate an optimal condition for shoot proliferation and regenerate shoots from in vitro leaflet and embryogenic calli from in vitro roots in rose. The effect of BAP on shoot proliferation was somewhat different depending upon genotypes or gelling agents. Leaflets with petiole cut from donor shoots which had been cultured in MS medium supplemented with 0.1 $mg{\cdot}L^{-1}$ NAA for six weeks was effective for regeneration of adventitious buds(ABs) as well as shoot elongation of Rosa hybrida cv. Sweet Pink. Culturing seven leaflet explants per petri plate($100mm{\times}15mm$) was effective for regeneration of ABs. Embryogenesis was shown in the calli induced from roots of Rosa hybrida cv. Sweet Pink cultured in the SH medium supplemented with 11 $mg{\cdot}L^{-1}$ 2, 4-D for four weeks. Color of calli induced from roots was yellow although their color was a little different as type of basal medium.

The Development of Early Mouse Embryos Depend on $Ca^{2+}$ Concentration (Ca$^{2+}$ 농도에 따른 생쥐 초기배의 발생)

  • Yang, Jung-Sook;Bae, In-Ha
    • Clinical and Experimental Reproductive Medicine
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    • v.27 no.3
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    • pp.275-282
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    • 2000
  • Objective: This study was to determine the effect of different concentration of calcium III medium on the preimplantational development of zygotes and early 2 cell embryos. Methods: Female mice of ICR strain ($5{\sim}8$ weeks old) were superovulated and mated with fertile males. Zygotes or early 2-cell embryos were collected by flushing the oviducts $31{\sim}32$ hours after hCG injection. The embryos were cultured in various concentrations of $Ca^{2+}$ in medium or with EDTA, EGTA and $Ni^{2+}$. Result and Conclusion: Treatment of high concentraion of $Ca^{2+}$ (3.42 mM $(2X){\sim}17.l$ mM (10X)) in medium didn't develop well compared to the control. Low concentrations of $Ca^{2+}$ (0.214mM $(1/8X){\sim}0.855$ mM (1/2X) were deterimental to development beyond 2-cell stage. EDTA, $Ca^{2+}$ chelating agent was treated with ranged concentrations of EDTA (0.014 $mM{\sim}0.107$ mM) to medium contaning 1.71 mM $Ca^{2+}$ showed beneficial effect to development to blastocyst compared to the control. EGTA, extracellular $Ca^{2+}$ chelator, was treated with ranged concentrations of EGTA ($0.014{\sim}0.107$ mM) to the medium contaning 1.71 mM $Ca^{2+}$. There is no significant difference with the control. $Ni^{2+}$ (50 ${\mu}M$), T-type $Ca^{2+}$-channel blocker was treated to medium contaning low concentration of $Ca^{2+}$. It overcame 2-cell block significantly. Rate of degenerated embryos decreased and developmental rate to morula and blastocyst increased more than low $Ca^{2+}$ concentration alone. Further studies are needed for the overcoming effect of 2-cell block by $Ni^{2+}$.

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EFFECTS OF OVARY TYPE, OOCYTE GRADE, HORMONE, SPERM CONCENTRATION AND FERTILIZATION MEDIUM ON IN VITRO MATURATION, FERTILIZATION AND DEVELOPMENT OF BOVINE FOLLICULAR OOCYTES

  • Im, K.S.;Kim, H.J.;Chung, K.M.;Kim, H.S.;Park, K.W.
    • Asian-Australasian Journal of Animal Sciences
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    • v.8 no.2
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    • pp.123-127
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    • 1995
  • In vitro embryo production (IVP) is affected by various factors during in vitro maturation, fertilization, and development. In this experiment, the effect of ovary type, quality of follicular oocyte, medium used for fertilization, presence of hormone in medium, sperm concentration on in vitro maturation and fertilization were examined for effective IVP. In vitro maturation was carried out using TCM-199 supplemented with 15% FCS and hormones in 5% $CO_2$ incubator for 24h. In vitro fertilization was performed with frozen-thawed sperm in modified mTALP medium containing 0.3% BSA, $10{\mu}g/ml$ heparin, and 5mM/ml caffeine for 24h. The fertilized embryos were co-cultured on monolayer of cumulus cells in TCM-199. When oocytes were collected from functionally active and inactive ovaries, maturation rate was 76.9 and 7.7%, respectively. When oocytes were classified morphologically to good and poor grades, maturation rate was 75 and 58.8%, respectively. FSH + LH + $E_2$ (86.4%) showed higher maturation rate than control (53.0%) and FSH (73%). The fertilization rate was 28.2, 100 and 91.7% in $1.6{\times}10^5$, $5.0{\times}10^5$ and $10.0{\times}10^5$ sperm concentration per ml. When oocytes were fertilized in mTALP and BO media, fertilization and cleavage rates of oocytes in mTALP were higher (84.3 and 56.9%) than those (67.4 and 23.3%) in BO medium. In this experiment, in vitro maturation, fertilization and development of oocytes were affected by type of ovary, grade of oocyte, hormones, sperm concentration and fertilization medium.

Study of the Production of Alkaline Keratinases in Submerged Cultures as an Alternative for Solid Waste Treatment Generated in Leather Technology

  • Cavello, Ivana A.;Chesini, Mariana;Hours, Roque A.;Cavalitto, Sebastian F.
    • Journal of Microbiology and Biotechnology
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    • v.23 no.7
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    • pp.1004-1014
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    • 2013
  • Six nonpathogenic fungal strains isolated from alkaline soils of Buenos Aires Province, Argentina (Acremonium murorum, Aspergillus sidowii, Cladosporium cladosporoides, Neurospora tetrasperma, Purpureocillium lilacinum (formerly Paecilomyces lilacinus), and Westerdikella dispersa) were tested for their ability to produce keratinolytic enzymes. Strains were grown on feather meal agar as well as in solid-state and submerged cultures, using a basal mineral medium and "hair waste" as sole sources of carbon and nitrogen. All the tested fungi grew on feather meal agar, but only three of them were capable of hydrolyzing keratin, producing clear zones. Among these strains, P. lilacinum produced the highest proteolytic and keratinolytic activities, both in solid-state and submerged fermentations. The medium composition and culture conditions for the keratinases production by P. lilacinum were optimized. Addition of glucose (5 g/l) and yeast extract (2.23 g/l) to the basal hair medium increased keratinases production. The optimum temperature and initial pH for the enzyme production were $28^{\circ}C$ and 6.0, respectively. A beneficial effect was observed when the original concentration of four metal ions, present in the basal mineral medium, was reduced up to 1:10. The maximum yield of the enzyme was 15.96 $U_c/ml$ in the optimal hair medium; this value was about 6.5-fold higher than the yield in the basal hair medium. These results suggest that keratinases from P. lilacinum can be useful for biotechnological purposes such as biodegradation (or bioconversion) of hair waste, leading to a reduction of the environmental pollution caused by leather technology with the concomitant production of proteolytic enzymes and protein hydrolyzates.

Effects of Hexoses on In Vitro Development of Parthenogenetic Embryos in the Pigs (돼지 단위발생란의 체외발육시 6탄당의 영향)

  • Yoon S. Y.;Kim C. I.;Cheong H. T.;Yang B. K.;Park C. K.
    • Journal of Embryo Transfer
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    • v.20 no.2
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    • pp.113-121
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    • 2005
  • This study was conducted to investigate the effects of different hexoses (glucose, mannose, galactose and fructose) on in vitro development of parthenogenetic embryos in the pigs. When the parthnogenetic embryos were cultured in medium with concentrations of 5mM glucose or 1mM galactose, the rates of embyos developed to morula and blastocyst stages were significantly higher than those in another culture conditions (P<0.05). However, high concentration of galactose inhibited development to morula and blastocyst stages. Addition of hexoses at early stage of porcine parthenogenetic embryos were effective for in vitro development. Especially, the embryos cultured in medium with glucose at early stage were effective for development to 2-cell $(72\%)$ and blastocyst $(19\%)$ stages compared with embryo cultured without glucose. From the present results, it is suggested that development of porcine parthenogenetic embryos can improve in medium with 5mM glucose. The concentration of 1mM galactose was also effective for development of porcine parthenogenetic embryos. It also show that parthenogenetic embryos cultured with glucose at early stage can improve in vitro development.

Bioalcohol Production with Microalgae, Microcystis aeruginosa (미세조류 Microcystis aeruginosa로부터 바이오 알콜의 생산)

  • Kim, Jong Deog;Chae, Go Woon;Seo, Hyo Jin;Chaudhary, Narendra;Yoon, Yang Ho;Shin, Tai Sun;Kim, Min Yong
    • KSBB Journal
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    • v.27 no.6
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    • pp.335-340
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    • 2012
  • The microalgae, Microcystis aeruginosa are able to proliferate in a wide range of freshwater ecosystem. M. aeruginosa was cultivated in 25 L and 240 L race-way reactor containing modified medium with added urea 0.2 g/L, increased $Fe^{+2}$, and decreased $Ca^{+2}$ion compared to BG11 medium. Sugar contents of M. aeruginosa grown in BG11 medium, and modified medium were 120 mg/mL and 140 mg/mL respectively. Fermentation was conducted with the extract of M. aeruginosa at $30^{\circ}C$ for 30 h, using Saccharomyces cerevisiae (Sc), Pichia stipitis (Ps), Zymomonas mobilis (Zm), and mixed-culture of these strains (Sc + Ps + Zm). Pichia stipitis (0.7%) was found to be more suitable for producing bioalcohol from M. aeruginosa extract than other strains of Saccharomyces cerevisiae (0.45%) and Zymomonas mobilis (0.61%), while mixed-cultured of these strains showed higest productivity by 1.75%. Biomass of M. aeruginosa contains the potency to be the most renewable resource for bioalcohol fermentation.