• Title, Summary, Keyword: Melaleuca Alternifolia

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Antimicrobial Effects of Tea Tree (Melaleuca alternifolia) Extracts (티트리 (Melaleuca alternifolia) 추출물의 항균 활성)

  • Ji, Keunho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.5
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    • pp.102-108
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    • 2019
  • Melaleuca alternifolia contains terpineol-4, cineol, sesquiterpenes etc., and has a germicidal effect and skin moisturizing effect. It also has the characteristics of relieving acne inflammation, treating dandruff, relieving pain, and relieving depression. In this study, an antimicrobial substance extracted from tea tree using an organic solvent (methanol, dichloromethane, ethyl acetate) and hydrothermal extraction method. And confirmed the antimicrobial activity of each extract. In order to verify the antimicrobial activity, nine pathogenic bacteria (Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa and Vibrio parahaemolyticus) were used. The antimicrobial activity of each extracts were confirmed by the commonly used disc diffusion method. The results showed that the fraction extracts of ethyl acetate and methanol had antimicrobial effects against V. parahaemolyticus and S. aureus. Using these results, we confirmed the antimicrobial activity of each fraction extracts and hot water extracts against V. parahaemolyticus. After the treat of samples, we confirmed at over 99.9 % of antimicrobial activity. In case of antifungal activities, we confirmed of preservation effect during over 45 hours. Based on the results of this research, further studies will be conducted to confirm the possibility of development as a new antimicrobial agent.

Antimicrobial activity of fraction mixture of ethanol extracts from Eucalyptus globulus, Yucca recurvifolia, and Melaleuca alternifolia against several human skin microbes (유칼립투스, 유카와 차나무의 추출분획 혼합물의 여러 인간 피부 상재균에 대한 항균활성)

  • Lee, Da-Sol;Hong, In Kee;Song, Hong-Gyu
    • Korean Journal of Microbiology
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    • v.55 no.1
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    • pp.46-51
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    • 2019
  • This study was carried out to evaluate antimicrobial effects of a mixture of resin fractionated ethanol extract of Eucalyptus globulus, Yucca recurvifolia, and tea tree (Melaleuca alternifolia). The plant fraction mixture showed low minimum inhibitory concentration (0.24~3.32 mg/ml) against several bacteria and yeast that usually used as the target skin microbes in a cosmetic industry, and it was more effective than antibiotics, triclosan and ampicillin. In a time-kill assay the plant fraction mixture reduced more than 92% of microbial populations during 4 h, and significantly increased leakage of nucleotides from all microorganisms tested. Antimicrobial effect of the plant fraction mixture was not affected by divalent cation ($Mg^{2+}$ and $Ca^{2+}$). These results suggest that the fraction mixture of ethanol extracts of E. globulus, Y. recurvifolia, and M. alternifolia may be utilized as an efficient preservative in cosmetics to prevent contamination by human skin microbes.

Bioactivity and Chemical Composition of the Essential oil of Tea Tree (Melaleuca alternifolia) (티트리 에센셜오일의 생물활성 및 주요 성분 분석)

  • Yang, Seun-Ah;Jeon, Sang-Kyung;Lee, Eun-Jung;Im, Nam-Kyung;Jung, Ji-Young;Lee, In-Seon
    • Journal of Life Science
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    • v.18 no.12
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    • pp.1644-1650
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    • 2008
  • The essential oil of tea tree (Melaleuca alternifolia) is widely used in traditional Australian medicine for skin lesions and infected injuries. In the present study, we investigated the chemical composition, cytotoxicity and its biological activities. The composition of the oil was analyzed by GC-MS. ${\beta}$-Terpinene (20.87%), ${\alpha}$-pinene (17.60%), p-cymene (11.23%), 3-carene (10.40%), trans-anethole (8.47%) and limonene (4.65%) were the major components in the oil. The results tested by MTT assay indicated that the oil showed no cytotoxic effect, at concentrations up to 5%, for less than 3h. The antiradical capacity was evaluated by measuring the scavenging activity of the essential oil on the 2,20-diphenylpicrylhydrazyl (DPPH) and 2,2'-azino-bis 3-ethyl benzothiazoline-6-sulfonic acid (ABTS) radicals. The oil was able to reduce the both radicals dose-dependently, and the concentration required for 50% reduction ($RC_{50}$) against ABTS radicals ($1.6{\pm}0.02%$) was slightly lower than DPPH radicals ($2.6{\pm}0.29%$). The direct contact and vapor-phase antibacterial activity of the oil were also evaluated using disc diffusion method against Staphylococcus aureus, Streptococcus mutans, Listeria monocytogenes, Acinetobacter baumannii, Escherichia coli, and Vibrio parahaemolyticus. All the Gram-negative bacterial strains tested showed more sensibility to the oil than the Gram-positive strains when compare to the effect of gentamycin. On the other hand, the vapor phase of the essential oil against S. aureus exhibited strongest inhibitory effect.

Inhibitory effect of Plant Essential Oils on Malassezia pachydermatis

  • Lee, Jeong-Hyun;Lee, Jae-Sug
    • Journal of Applied Biological Chemistry
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    • v.53 no.3
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    • pp.184-188
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    • 2010
  • Effect of the plant essential oils on the growth of Malassezia pachydermatis was evaluated and the essential oils of Ocimum basilicum L., Melaleuca alternifolia (Maid. & Bet.) Cheel, and Rosa damascene Mill. were the most active against M. pachydermatis and their activity were high than that of itraconazole at 2 mg/mL. The major constituents of the three oils by GC-MS analysis were linalool (21.83%) and estragole (74.29%) for O. basilicum, a-terpinolene (17.96%) and terpinen-4-01 (45.54%) for M. alternifolia, and a-citronellol (59.98%) and geraniol (27.58%) for R. damascene. Results showed that these selected three oils could be effective toward controlling M. pachydermatis opportunistic infections.

Growth-Inhibiting Effects of Herb Plants on Human Intestinal Bacteria

  • Kim, Moo-Key;Park, Byeoung-Soo;Kim, Byung-Su;Lee, Hoi-Seon
    • Journal of Applied Biological Chemistry
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    • v.44 no.4
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    • pp.185-189
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    • 2001
  • Essential oils of 21 herb plant samples, using spectrophotometric and paper disc agar diffusion methods under anaerobic conditions, were tested in vitro for their growth-inhibiting activities against Bifidobacterium bifidum, B. longum, Lactobacillus casei, Clostridium perfringens, and Escherichia coli. The responses varied with bacterial strains and plant oils. At 10 mg/disk, all essential oils did not inhibit beneficial intestinal bacteria, except for the oil of Alpinia officinarum and Melaleuca alternifolia against L. casei. Due to their strong growth-inhibitory activities against C. perfringens, E. coli, and L. casei, the activites of nine oils were evaluated at low concentrations. In test with C. perfringens at 1 mg/disk, the oils of Amyris balsamifera, Curcuma longa, M. alternifolia, and Trachyspermum ammi showed moderate activities. Moderate activities against E. coli were observed with the oils of M. alternifolia and T. ammi. These results may be indications of at least one of the pharmacological actions of the four herb plants.

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Insecticidal toxicities of essential oils extracted seven plants against Ricania sp. nymphs and adults (돌발 해충, 갈색날개매미충에 대한 7종 식물체 오일성분의 살충)

  • Jeon, Ye-Jin;Choi, Byeong-Ryeol;Lee, Hoi-Seon
    • Journal of Applied Biological Chemistry
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    • v.59 no.3
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    • pp.243-245
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    • 2016
  • The insecticidal toxicities of seven essential oils against Ricania sp. nymph and adults, newly recorded pest, were evaluated using leaf-dipping and spray methods. At 72 h exposure time, the $LC_{50}$ values of the essential oils of Artemisia vulgaris, Anethum graveolens, Citrus paradisi, Melaleuca alternifolia, Melaleuca leucadendron, Neroli bigarade and Tagetes erecta were 251.02, 387.31, 286.21, 353.54, 275.64, 424.55, $165.68mg/cm^2$ when using the leaf dipping method against Ricania sp. nymphs, and 278.31, 402.11, 306.11, 379.64, 298.22, 459.56, $183.21mg/cm^2$ when using the spray method against Ricania sp. adults, respectively. These results indicate that the essential oil of the Tagetes erecta can be potentially used as new natural insecticidal agents against Ricania sp. nymph and adults.

Comparision of Anti-microbial Oils as Natural Preservatives (천연방부제로서 항균오일의 항균력 비교)

  • Kim, Mi-Jin;Jung, Taek-Kyu;Hong, In-Gi;Yoon, Kyung-Sup
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.32 no.2
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    • pp.99-103
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    • 2006
  • Natural essential oils showed anti-microbial activity on relatively broad spectrum of bacterial and fungal species. These materials had much more intensive anti-microbial activities than synthetic preservatives on C. albicans, A niger, and P. acnes, especially. In the experimental group, anti-microbial activity was order of tea tree oil (from Melaleuca alternifolia) > methylparaben > phytoncide (from Chamaecyparis obtusa). Also, natural essential oils had anti-oxidative and anti-inflammatory effects. These results suggest that natural essential oils can be useful as good cosmetic ingredients such as natural preservatives and anti-oxidants.

Anti-Aspergillus Activities of Plant Essential Oils and Their Combination Effects with Ketoconazole or Amphotericin B

  • Shin, Seung-Won
    • Archives of Pharmacal Research
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    • v.26 no.5
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    • pp.389-393
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    • 2003
  • The essential oils from Cedrus atlantica, Styrax tonkinensis, Juniperus communis, Lavandula angustifolia, Melaleuca alternifolia, Pelargonium graveolens, Pogestemon patchouli and Rosmarinus officinalis were analyzed by GC-MS. Antifungal activities of the oils were investigated by disk diffusion assay and the broth dilution method against Aspergillus niger and A. flavus. The effects of geraniol and the essential oil fraction from P. graveolens on the antifungal activity of amphotericin Band ketoconazole were examined using a checkerboard microtiter assay against both Aspergillus fungi. Most of the tested essential oils, with the exception of C. atlantica, J. communis, and P. patchouli, significantly inhibited growth of A. niger and to a lesser extent that of A. fIavus, with MICs (minimal inhibitory concentrations) in the range 0.78-12.5 mg/mL. The essential oil fraction of P. graveolens and its main components, geraniol and citronellol, exhibited additive effects with amphotericin B and with ketoconazole against both Aspergillus species, resulting in fractional inhibitory concentration (FIC) indices ranging from 0.52 to 1.00.

Effects of A-solution on Halitosis and Oral Status in Preoperative NPO Patients (A-solution을 이용한 구강 가글링이 수술 전 금식 환자의 구취와 구강 상태에 미치는 효과)

  • Song, Ji-Ah;Hur, Myung-Haeng
    • Journal of Korean Academy of Nursing
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    • v.42 no.3
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    • pp.405-413
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    • 2012
  • Purpose: The aim of this study was to explore the effects of A-solution on halitosis and oral status in preoperative NPO patients. Methods: A nonequivalent control group, non-synchronized pretest-posttest design was used in this study. The participants in this study were 66 patients who were admitted for gynecologic surgery. The experimental treatment was to give oral gargling with A-solution, blended essential oils and diluted with distilled water. To identify the experimental treatment effects, halitosis, salivary pH, and oral status were measured by a portable halitosis detector, visual analogue scale, Bromo Thymol Blue (BTB), Bromo Cresol Purple (BCP) test paper and oral assessment guide. Data were analyzed using $X^2$-test, t-test with PASW 18.0 version. Results: Participants were homogeneous before experimental treatment. Objective halitosis in the experimental group, measured by a portable halitosis detector (t=-8.34, p<.001) was significantly lower than the control group. Subjective halitosis was significantly lower in the experimental group (t=-9.29, p<.001). Salivary pH was significantly different between two groups (t=8.81, p<.001). Oral status was significantly better in the experimental group (t=-13.31, p<.001). Conclusion: These findings indicate that oral gargling using A-solution is effective in reducing patient halitosis, and improving oral status.

Clinical Assessment on the Safety of Acupuncture with Aromatherapy (향기침요법(Acupuncture with Aromatherapy)의 임상적인 부작용에 대한 연구)

  • Hong Jin-Woo;Kim Chang-Hyun;Min In-Kyu;Chung Sae-Yun;Hwang Jae-Woong;Kim Seok-Min;Sun Jong-Joo;Jung Jae-Han;Choi Chang-Min;Jung Woo-Sang;Moon Sang-Kwan;Cho Ki-Ho;Kim Young-Suk
    • The Journal of Korean Medicine
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    • v.27 no.2
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    • pp.96-102
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    • 2006
  • Background & Purpose : Acupuncture with aromatherapy (AA) is a branch of complementary or alternative therapy recently growing in use. As the use of aromatherapy itself has grown so rapidly in recent years, studies about its safety have been accumulating. However, safety of AA has not been studied clinically and the treatment method which has the needles inserted after spreading essential oils exposes the human body more directly to components than aromatherapy. Therefore, we examined the safety of AA on patients treated thereby and physicians practising it. Methods : We observed all inpatients treated with AA from November 21, 2005 to March 31, 2006, in Kyung Hee Oriental Medical Center. During treatment, we monitored all of the newly developed signs from the patients to assess the adverse effects of AA. The patients' and physicians' general characteristics (gender, age, present illness, and treatment days) were obtained from medical records and analyzed to assess the clinical safety of AA in detail. Results : There were 440 patients treated with AA; clinical adverse effects appeared in 3 of them, which can be assessed as 0.7%. The major symptom was chest discomfort and choking originated from smell aversion condition (2 patients), and the other adverse effect was sensation of itching and reddening. However, none of them seemed to have direct relation with AA. A total of eight physicians applied AA and there were no symptoms related to it among them. Conclusions : We suggest that our results will contribute to confirming the safety of AA by offering clinical evidence.

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