• Title, Summary, Keyword: Middle Cerebral Artery Occulsion(MCAO)

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The Protective Effect of Hirudin Herbal-acupuncture against the Neuronal Damage Induced by Middle Cerebral Artery Occulsion(MCAO) in Rats (Hirudin 약침(藥鍼)이 중대뇌동맥폐색(中大腦動脈閉塞)으로 유발(誘發)된 흰쥐의 신경손상(神經損傷) 보호(保護) 효과(效果))

  • Suk, Jae-wook;Jung, Tae-young;Lim, Seong-cheol;Seo, Jeong-chul;Kim, Mi-ryeo;Yang, Chae-ha;Han, Sang-won
    • Journal of Acupuncture Research
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    • v.21 no.4
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    • pp.207-215
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    • 2004
  • Objective : In order to prove the effect of Hirudin Herbal-acupuncture this experimental studies were performed by using rats that had neuronal damage due to the Middle Cerebral Artery Occulsion(MCAO). Methods : Microdialysis probes were implanted into the coordinate of striatum of anesthetized rats which consist of sham-operated 8 rats, MCAO-operated 8 rats and Hirudin Herbal-acupuncture administrated 8 rats before MCAO operating. The Hirudin Herbal-acupuncture(0.5mg/kg) was administrated to rats 30 minutes before having an operation causing the MCAO. The surgical excision lead the cross resected brain to the acute ischemic state. The brain was sliced in 2mm thickness and stained with cresyl violet buffer for the measurement of cerebral infarcted area and volume. Results : Based on the result of the tissue inspection for the cerebral ischemic cell, Hirudin Herbal-acupuncture significantly protect neurocytes. Conclusion : We suggest Hirudin Herbal-acupuncture produces protective effects against the neuronal damage induced by MCAO. Therefore, Hirudin Herbal-acupuncture may prevent delayed neuronal death(DND) in selectively vulnerable focal areas of the brain effectively.

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The Effect of Hirudin Herbal-acupuncture on Neurotransmitters against Middle Cerebral Artery Occulsion(MCAO) Rats (Hirudin 약침(藥鍼)이 뇌허혈(腦虛血)을 유발(誘發)시킨 흰쥐의 신경전도물질(神經傳達物質)에 미치는 영향(影響))

  • Suk, Jae-Wook;Jung, Tae-Young;Leem, Seong-Cheol;Seo, Jeong-Chul;Han, Sang-Won
    • Journal of Pharmacopuncture
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    • v.7 no.2
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    • pp.29-41
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    • 2004
  • Objective : This experimental studies were performed in order to prove the effect of Hirudin Herbal-acupuncture by using rats that had neuronal damage due to the Middle Cerebral Artery Occulsion(MCAO). Method : We observed the change of extracellular concentrations(${\mu}M$) of dopamine, DOPAC, HVA, HIAA, glutamate, aspartate, GABA, glysine, taurine, alanine, and tyrosine as extracted by vivo microdialysis, in the Hirudin Herbal-acupuncture administrated rats($240{\sim}260g$, Sprague-Dawley) subjected to the MCAO. The dialysates were extracted three times before the MCAO and six times after the MCAO every 20 minutes, and analysed by highperformance liquid chromatography(HPLC). Results : Hirudin Herbal-acupuncture significantly inhibited glutamate, aspartate, and tyrosine which are stimulant neurotransmitters at brain ischemia, and it significantly decreased glycine, GABA, taurine, and alanine which are inhibitory neurotransmitters at brain ischemia. Conclusion : Hirudin Herbal-acupuncture may prevent delayed neuronal death(DND) in selectively vulnerable focal areas of the brain effectively.

The Effect of Phospholipase $A_2$ Herbal-acupuncture on Neurotransmitters against Middle Cerebral Artery Occulsion Rats. (Phospholipase $A_2$ 약성이 뇌허혈을 유발시킨 흰쥐의 신경전달물질에 미치는 영향)

  • Kim Sung Min;Jung Tae Young;Leem Seong Cheol;Seo Jeong Chul;Han Sang Won
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.18 no.5
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    • pp.1322-1330
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    • 2004
  • This experimental studies were performed in order to prove the effect of Phospholipase A₂(PLA₂) Herbal-acupuncture by using rats that had neuronal damage due to the Middle Cerebral Artery Occulsion(MCAO). We observed the change of extracellular concentrations(μM) Of dopamine, DOPAC, HVA, HIAA, glutamate, aspartate, GABA, glysine, taurine, alanine, and tyrosine as extracted by vivo microdialysis, in the PLA₂ Herbal-acupuncture administrated rats(240-260g, Sprague-Dawley) subjected to the MCAO. The dialysates were extracted three times before the MCAO and six times after the MCAO every 20 minutes, and ana lysed by highperformance liquid chromatography(HPLC). PLA₂ Herbal-acupuncture significantly inhibited glutamate and tyrosine which are stimulant neurotransmitters at brain ischemia, and it significantly decreased glycine, GABA, taurine, and alanine which are inhibitory neurotransmitters at brain ischemia. PLA₂ Herbal-acupuncture may prevent delayed neuronal death(DND) in selectively vulnerable focal areas of the brain effectively.

The Protective Effect of Phospholipase $A_2(PLA_2)$ Herbal-acupuncture against the Neuronal Damage Induced by Middle Cerebral Artery Occulsion(MCAO) in Rats. (Phospholipase $A_2(PLA_2)$ 약침(藥鍼)이 중대뇌동맥폐색(中大腦動脈閉塞)으로 유발(誘發)된 흰쥐의 신경손상(神經損傷) 보호(保護) 효과(效果)에 미치는 영향)

  • Kim, Sung-Min;Jung, Tae-Young;Leem, Seong-Cheol;Seo, Jeong-Chul;Kim, Mi-Ryeo;Yang, Chae-Ha;Han, Sang-Won
    • Korean Journal of Acupuncture
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    • v.21 no.3
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    • pp.89-96
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    • 2004
  • Objectives : In order to prove the effect of Phospholipase $A_2(PLA_2)$ Herbal-acupuncture, this experimental studies were performed by using rats that had neuronal damage due to the Middle Cerebral Artery Occulsion(MCAO). Methods : Microdialysis probes were implanted into the coordinate of striatum of anesthetized rats which consist of sham-operated 8 rats, MCAO-operated 8 rats and $PLA_2$ Herbal-acupuncture administrated 8 rats before MCAO operating. The $PLA_2$ Herbal-acupuncture(0.5mg/kg) was administrated to rats 30 minutes before having an operation causing the MCAO. The surgical excision lead the cross resected brain to the acute ischemic state. The brain was sliced in 2mm thickness and stained with cresyl violet buffer for the measurement of cerebral infarcted area and volume. Results : Based on the result of the tissue inspection for the cerebral ischemic cell, $PLA_2$ Herbal-acupuncture significantly protect neurocytes. Conclusions : We suggest $PLA_2$ Herbal-acupuncture produces protective effects against the neuronal damage induced by MCAO. Therefore, $PLA_2$ Herbal-acupuncture may prevent delayed neuronal death(DND) in selectively vulnerable focal areas of the brain effectively.

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