• Title, Summary, Keyword: Mouse

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Mouse Strain-Dependent Osteoclastogenesis in Response to Lipopolysaccharide

  • Choi, Ho-Gil;Kim, Jin-Moon;Kim, Bong-Ju;Yoo, Yun-Jung;Cha, Jeong-Heon
    • Journal of Microbiology
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    • v.45 no.6
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    • pp.566-571
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    • 2007
  • Bacterial lipopolysaccharide (LPS) is a potent stimulator of bone resorption in periodontitis. Co-culture systems of mouse calvaria-derived osteoblasts and bone marrow-derived preosteoclasts were used as an in vitro osteoclast differentiation. This study revealed that co-cultures using ddY or ICR mouse strain responded differently to LPS while responded equally to $1{\alpha},25(OH)_2D_3$. Thus, the different response to LPS indicates dissimilarity of two mouse stains in their capacity for generating osteoclasts while the two mouse strains share the similarity in response to $1{\alpha},25(OH)_2D_3$. To identify which cells between osteoblasts and preosteoclasts in the co-culture are responsible for the dissimilarity, the reciprocal co-cultures were performed between ddY and ICR mouse strains. The treatment of $1,25(OH)_2D_3$ to ddY/ICR (osteoblasts from ddY/preosteoclasts from ICR) and ICR/ddY reciprocal co-cultures also showed the similarity. In case of LPS treatment, the results of ddY/ICR were similar to ddY/ddY and the results of the other reciprocal co-culture, ICR/ddY combination, were consistent with those of ICR/ICR. It suggests that the dissimilarity between the two mouse strains may resident in osteoblasts but not in preosteoclasts. Therefore, the osteoblast is responsible for mouse strain-dependent osteoclastogenesis in response to LPS. Although mouse models will continue to provide insights into molecular mechanisms of osteoclastogenesis, caution should be exercised when using different mouse strains, especially ddY and ICR strains as models for osteoclast differentiation.

Studies on the Effects of the Saponin Fraction of Panax Ginseng on the Immune System of the Mouse Fed with Protein Malnutritive Diet (인삼사포닌 분획이 단백부족 마우스의 면역체계에 미치는 영향)

  • 이나경;김영중
    • YAKHAK HOEJI
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    • v.30 no.4
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    • pp.174-179
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    • 1986
  • The thymus weight of the mouse was 54.1% in protein malnutritive diet group and 39.2% in group treated with saponin fraction of Panax ginseng in comparison to normal diet group. This decreasing effects of protein malnutritive diet and saponin fraction on the thymus weight practically disappeared after four weeks. The saponin fraction showed no effect on the spleen weight of the mouse. The supplement of the saponin fraction enhanced total peritoneal exudate cells, content of total serum protein and albumin content of the mouse, each 45, 8 and 10% respectively in comparision to that of normal diet group. And these values in protein malnutritive diet group were 61.2, 83.6 and 87.0% respectively in comparision to that of normal diet group, and recovered to the level of normal diet group by the supplement of the saponin fraction. The electrophoregram of the serum protein of the mouse fed with protein malnutritive diet was different from that of the mouse fed with normal diet, but this difference practically disappeared by the supplement of the saponin fraction.

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Mouse models for hepatitis B virus research

  • Hwang, Jeong-Ryul;Park, Sung-Gyoo
    • Laboraroty Animal Research
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    • v.34 no.3
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    • pp.85-91
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    • 2018
  • Hepatitis B virus (HBV) infection remains a major global health problem; indeed, there are 250 million carriers worldwide. The host range of HBV is narrow; therefore, few primates are susceptible to HBV infection. However, ethical constraints, high cost, and large size limit the use of primates as suitable animal models. Thus, in vivo testing of therapies that target HBV has been hampered by the lack of an appropriate in vivo research model. To address this, mouse model systems of HBV are being developed and several are used for studying HBV in vivo. In this review, we summarize the currently available mouse models, including HBV transgenic mice, hydrodynamic injection-mediated HBV replicon delivery systems, adeno-associated virus-mediated HBV replicon delivery systems, and human liver chimeric mouse models. These developed (or being developed) mouse model systems are promising and should be useful tools for studying HBV.

Fertilization Processes in Porcine Oocytes Following Intracytoplasmic Injection of Porcine and Mouse Spermatozoa

  • Lee, Youn-Jeung;Kim, Bong-Ki;Park, Chang-Sik;Kim, Nam-Hyung
    • Proceedings of the KSAR Conference
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    • pp.12-12
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    • 2001
  • To get insight into the nature of foreign mitochondria and syngamy during mammalian fertilization we compared fertilization processes in porcine oocytes following microinjection of porcine or mouse spermatozoa. Pronuclear movement, sperm mitochondria, and DNA synthesis were imaged with propidium iodide, mitotracker, and BrdU under confocal laser scanning microscope. Intracytoplasmic injection of either porcine or mouse spermatzoon activated porcine oocytes without additional parthengenetic stimulation. Foreign mitochondria in either mouse or porcine sperm midpiece were introduced into porcine oocytes following sperm injection, but rapidly disappeared from the actively developing porcine oocytes. BrdU experiment showed new DNA synthesis in porcine oocytes following injection of mouse spermatozoon or sperm head. At 24 h after injection of mouse isolated sperm head or a spermatozoon, mitoic metaphase was seen in oocyte, but they did not go to normal cell division (Table). These results suggest that pronuclear formation, foreign mitochondria disruption, DNA synthesis and syngamy formation during fertilization are not species specific processes.(Table Omitted).

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The Effects of T-2 Toxin on the Morphology of Mouse Organ (Mouse 장기의 형태에 미치는 T-2 Toxin의 영향)

  • 송재영;정덕화
    • Journal of Environmental Health Sciences
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    • v.21 no.4
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    • pp.96-101
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    • 1995
  • To study the immunotoxicity of mouse injected with fungal mycotoxin, T-2 toxin (Fusarium mycotoxin) was treated to 6 week-old female C3H/He mouse and the body, organ weight and morphological change were investigated. The weights of body, liver and kidney of mouse injected the 2 mg/kg of toxin was decreased to 17, 20 and 3%, respectively, compared to control animal and the comsumption of feed was also decreased with lapsing the time. The fat dropleting phenomenon and destruction of Golgi apparatus in liver and histopathological changes of tissue and mitochondria in small intestine were found by scanning electron microscopic observation.

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Chemical Synthesis and Determination of Biological Activity of the Epidermal Growth Factor-Like Domain of Mouse Betacellulin

  • Shin, Song-Yub;Kang, Shin-Won;Ha, Jong-Myung
    • BMB Reports
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    • v.28 no.2
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    • pp.87-93
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    • 1995
  • To investigate the biological functions of the EGF-like domain of mouse betacellulin (BTC), mouse BTC(33-80), a 48-residue peptide corresponding to the EGF-like domain, was synthesized by stepwise solidphase methods using a 9-fluorenylmethoxycarbonyl (Fmoc) strategy. The homogeneity of synthetic mouse BTC(33-80) was confirmed by analytical reversed phase (RP)-HPLC, amimo acid analysis, and fast atom bombardment mass spectrometer (FAB-MS). Three disulfide bond pairings of synthetic mouse BTC(33-80) were established by amino acid analysis of cysteine-containing fragments derived from thermolytic digestion. These were consistent with the pairings of EGF and transforming growth factor ($TGF-{\alpha}$). The EGF-Iike domain of mouse BTC showed equipotent activity in both EGF-receptor binding on A-431 epidermoid carcinoma cells, and mitogenesis on NIH-3T3 fibroblast cells, as compared with authentic h-EGF. Results suggest that the EGF-Iike domain of BTC plays a significant role in mitogenic activity with an EGF-receptor mediated system.

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An Interactive Game with a Haptic Mouse (햅틱마우스를 이용한 인터랙티브 게임)

  • Cho, Seong-Man;Jung, Dong-June;Heo, Soo-Chul;Um, Yoo-Jin;Kim, Sang-Youn
    • 한국HCI학회:학술대회논문집
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    • pp.1-5
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    • 2009
  • In this paper, we develop a haptic mouse system for immersive human computer interaction. The proposed haptic mouse system can provide vibrotactile feedback as well as thermal feedback for realistic virtual experience. For vibrotactile and thermal feedback, we use eccentric motors, a solenoid, and a peltier actuator. In order to evaluate the proposed haptic mouse, we implement a racing game prototype system. The experimental result shows that our haptic mouse is expected to be useful in experiencing virtual world.

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A Study on Hair-growth Activity of Artemisia apiacea Hance (청호의 육모활성연구)

  • 김경순;심상희;장지면;정재훈;김박광
    • YAKHAK HOEJI
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    • v.43 no.6
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    • pp.798-801
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    • 1999
  • To investigate the effect of Artemisia apiacea Hance on the hair growth of black mouse (C57BL/6), this study was carried out. We obtained chloroform fraction, ethyl acetate fraction, butanol fraction from ethanol extract. When these fractions were administered to the black mouse by the method of skin paste, chloroform extract increased remarkably the hair growth of black mouse. thus Chloroform fraction was devided into 5 fractions from 20:1 to 1:1 with chloroform : methanol. the detail fractions of chloroform fraction were administered to the black mouse. We obtained the result that fraction I, II, III, IV are superior to minoxidil of positive control. Most of all fraction II have an dramatical effect on hair growth of black mouse.

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Effect of Biomechanical Intervention based on Custom Seating System on Activities of Mouse Click for Children with Cerebral Palsy (맞춤형 착석장치를 통한 생체역학적 중재가 뇌성마비 아동의 마우스 클릭 동작에 미치는 영향)

  • Jeong, Dong-Hoon
    • The Journal of Korean Physical Therapy
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    • v.24 no.2
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    • pp.57-65
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    • 2012
  • Purpose: This study was to investigate the effect of biomechanical intervention, based on the custom seating system on the activities of a mouse click for children with cerebral palsy. Methods: Thirteen children with cerebral palsy participated in this study. We compared reaction time and frequency for proper mouse click in the subject's typical position, in addition to an intervention position. The intervention position conformed to the principle and practice of research on promoting the upper-extremity movement and postural control. The intervention position was achieved through an external postural support, which was based on the custom seating system. Results: Reaction time and frequency for proper mouse click were moderately improved in the intervention position, compared with that of the typical position. There was a statistically significant difference between the typical position and that of the intervention position (p<0.05). Conclusion: Results provide evidence of the positive effects of functional seating on the activities of a mouse click for children with cerebral palsy.

Genetically Engineered Mouse Models for Drug Development and Preclinical Trials

  • Lee, Ho
    • Biomolecules & Therapeutics
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    • v.22 no.4
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    • pp.267-274
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    • 2014
  • Drug development and preclinical trials are challenging processes and more than 80% to 90% of drug candidates fail to gain approval from the United States Food and Drug Administration. Predictive and efficient tools are required to discover high quality targets and increase the probability of success in the process of new drug development. One such solution to the challenges faced in the development of new drugs and combination therapies is the use of low-cost and experimentally manageable in vivo animal models. Since the 1980's, scientists have been able to genetically modify the mouse genome by removing or replacing a specific gene, which has improved the identification and validation of target genes of interest. Now genetically engineered mouse models (GEMMs) are widely used and have proved to be a powerful tool in drug discovery processes. This review particularly covers recent fascinating technologies for drug discovery and preclinical trials, targeted transgenesis and RNAi mouse, including application and combination of inducible system. Improvements in technologies and the development of new GEMMs are expected to guide future applications of these models to drug discovery and preclinical trials.