• Title, Summary, Keyword: Neospora caninum

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Immunohistochemical and serological observations on Neospora caninum infection in Korean indigenous cattle and calves of abnormal deliveries (한우 및 이상산 송아지의 Neospora caninum의 감염에 대한 면역조직화학적 및 혈청학적 관찰)

  • 손성봉;정원일;정규식;이차수
    • Korean Journal of Veterinary Service
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    • v.27 no.1
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    • pp.53-62
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    • 2004
  • The present study was carried out to investigate the prevalence of Neospora caninum infection in Korean indigenous cattle and calves of abnormal deliveries and focus on correlation between malformation and N caninum infection. To determine the prevalence of antibodies to N caninum, sera of 473 Korean indigenous cattle from slaughter house were tested for N caninum antibodies using indirect fluorescence assay. Of the 473 cattle sera, 9.5% (45/473) showed positive against N caninum. Regional seropositive rates of the samples were 16.7% (5/85), 11.0% (11/100), 8.8% (21/240) and 5.9% (5/85) at Kyonggi, Gyeongbuk, Daegu and Kyongnam province, respectively. In female, seropositive rates were 17.5% (25/143) and 6.1% (20/330) in male. During the period from march 2000 to August 2001, 55 abnormal deliveries of Korean indigenous cattle including abortion, stillbirth and congenital malformation were examined by histopathological, immunohistochemical and serological methods for evidence of N caninum infection. Of the 55 abnormal deliveries, only 5 calves showed positive reaction against N caninum in serological test. In microscopical observation, gliosis and nonsuppurative myositis were observed. However, Neospora-like organisms were not detected by either periodic acid-schiff (PAS) reaction or immunohistochemical technique. Taken together all these data, this study indicate that N caninum infection was widespread in breeding farms of Korean native cattle, but correlation between malformation and N caninum infection was not recognized.

Fecal and Molecular Survey of Neospora caninum in Farm and Household Dogs in Mashhad Area, Khorasan Province, Iran

  • Razmi, Gholamreza
    • The Korean Journal of Parasitology
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    • v.47 no.4
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    • pp.417-420
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    • 2009
  • Neospora caninum is an important cause of abortion in dairy cattle worldwide. Dog is the definitive host for N. caninum and can infect dairy cattle. The aim of this study is to determine the prevalence of Neospora oocysts in feces of dogs from dairy farms. A total of 174 fecal samples was collected from 89 farm dogs and 85 household dogs during 2006 and 2008. Fecal samples of dogs were microscopically examined for detecting Hammondia Neospora-like oocysts (HNLO) by Mini $Parasep^{(R)}SF$ fecal parasite concentrator. HNLO were microscopically detected in 4 fecal samples (2.2%). The fecal samples with HNLO were examined by N. caninum-specific PCR. Two of the samples were positive for N. caninum. The 2 positive fecal samples were selected for inoculation to calves. Two inoculated calves were seronegative by ELISA for 4 months post-infection. This is the first report of finding N. caninum DNA in feces of farm dogs in Mashhad area, Iran.

Seroprevalence of Neospora caninum in dairy cattle of northern Gyeonggi province in Korea (경기도 북부지역 젖소의 Neospora caninum 항체 양성률 조사)

  • Jung, Kwang;Shim, Hang-Sub
    • Korean Journal of Veterinary Service
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    • v.36 no.1
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    • pp.53-56
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    • 2013
  • This study was carried out to investigate the seroprevalence of Neospora caninum infection in dairy cattle of northern Gyeonggi province in Korea. A total of 716 dairy cattle from 14 dairy farms were tested for the presence of antibodies against N. caninum using a commercial ELISA test kit. Herd and individual seroprevalences of N. caninum were 92.8% (13/14) and 23.1% (166/716), respectively. The seroprevalence of dairy cattle according to age was the highest at 49~72 months (27.4%), but the difference was not statistically significant (P>0.05). No significant relationship between N. caninum seropositivity with the herd size of the dairy farms was found (P>0.05). The results indicate that N. caninum infection spreads widely in dairy farms of northern Gyeonggi province in Korea.

Seroprevalence of Antibodies to Neospora caninum in Korean Native Cattle Raised in Kangwon Province (강원도 사육 한우에서 Neospora caninum에 대한 항체양성률 조사)

  • Hwang, Eui-kyung
    • Korean Journal of Veterinary Research
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    • v.43 no.2
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    • pp.283-288
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    • 2003
  • This survey was carried out to investigate the seroprevalence of antibodies to Neospora caninum in Korean native cattle (KNC) raised in Kangwon province in Korea. A total of 867 sera collected from KNC were tested for N. caninum antibodies using an indirect immunofluorescent antibody test (IFAT). One hundred and fifty five (17.9%) KNC were positive by IFAT. Seroprevalence of cows was 19.6% (44/224) and seroprevalence of boars was 17.3% (111/643). Among the seroprevalences of cattle according to the raised areas, five counties or cities, Hwacheon was 33.3% (1/3), Wonju was 30.8% (4/13), Chuncheon was 25.8% (24/93), Hongcheon 18.3% (22/120) and Wheongsung was 16.6%(104/628). It was proved that KNC raised in Kangwon provinces exposed extensively and seriously to N. caninum.

Seroprevalence of antibodies to Neospora caninum in dairy cattle raised in Kangwon province (강원도 사육 젖소의 네오스포라포자충(Neospora caninum)에 대한 항체양성률 조사)

  • Hwang, Eui-Kyung
    • Korean Journal of Veterinary Research
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    • v.50 no.1
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    • pp.19-24
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    • 2010
  • This survey was carried out to investigate the seroprevalence of antibodies to Neospora (N.) caninum in dairy cattle raised in Kangwon province in Korea. A total of 349 sera collected from dairy cattle were tested for N. caninum antibodies using an indirect immunofluorescent antibody test (IFAT). Ninety six (27.5%) dairy cattle were positive by IFAT. Seroprevalence of cows was 28.9% (91/315) and seroprevalence of boars was 14.7% (5/34). The seroprevalences of cows were increased according to the ages from 19.6% in cows less than 2 year-old to 50.0% in cows more than 7 year-old. Among the seroprevalences of cattle according to the raised areas, five counties or cities, Wheongsung was 34.6% (27/78), Wonju was 29.3% (46/157), Hongcheon was 28.9% (13/45), Chuncheon was 15.0% (3/20) and Cheolwon was 13.3% (2/15). It was proved that dairy cattle raised in Kangwon provinces exposed extensively and seriously to N. caninum.

Detection of Neospora caninum in the blood of Korean native cattle and dairy cows using PCR (한우와 젖소에서 PCR을 이용한 혈액내 Neospora caninum 검출)

  • Lee, Sang-Eun;Lee, Jung-Youn
    • Korean Journal of Veterinary Research
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    • v.48 no.2
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    • pp.191-195
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    • 2008
  • This study was performed to detect Neospora caninum in blood of 61 Korean native cattle and 50 dairy cows in Chungnam province. All of them were healthy and did not show any clinical signs. DNA was isolated from blood samples and a 328 bp fragment was amplified by PCR using primer pair Np21 and Np6. The PCR positive rate was 14.8% in Korean native cattle and 0% in dairy cows. Cows with 15.6% were a little higher than bulls with 12.5% in gender. The detection rate of over 3-year-old Korean native cattle was 28.6% in age. The results demonstrate that N. caninum DNA can be detected in blood by PCR. PCR analysis in blood may be useful to annually screening test for N. caninum infection in clinically healthy cattle.

Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against experimental Neospora caninum infection

  • CHO Jung-Hwa;CHUNG Woo-Suk;SONG Kyoung-Ju;NA Byoung-Kuk;KANG Seung-Won;SONG Chul-Yong;KIM Tong-Soo
    • The Korean Journal of Parasitology
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    • v.43 no.1
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    • pp.19-25
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    • 2005
  • Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against N. caninum infection was evaluated in vitro and in vivo. Two major immunodominant surface antigens (NcSAG1 and NcSRS2) and two dense granule proteins (NcDG1 and NcDG2) of N. caninum tachyzoites were expressed in E. coli, respectively. An in vitro neutralization assay using polyclonal antisera raised against each recombinant antigen showed inhibitory effects on the invasion of N. caninum tachyzoites into host cells. Separate groups of gerbils were immunized with the purified recombinant proteins singly or in combinations and animals were then challenged with N. caninum. Following these experimental challenges, the protective efficacy of each vaccination was determined by assessing animal survival rate. All experimental groups showed protective effects of different degrees against experimental infection. The highest protection efficacy was observed for combined vaccination with NcSRS2 and NcDG1. Our results indicate that combined vaccination with the N. caninum recombinant antigens, NcSRS2 and NcDG1, induces the highest protective effect against N. caninum infection in vitro and in vivo.

In vitro isolation of a bovine Neospora in Korea (국내 소에서 Neospora caninum의 분리)

  • Kim, Jae-hoon;Sohn, Hyun-joo;Hwang, Eui-kyung;Hwang, Woo-suk;Hur, Kwon;Jean, Young-hwa;Lee, Byung-chun;Rhee, Jae-chin;Kang, Yung-bai;Yamane, Itsuro;Kim, Dae-yong
    • Korean Journal of Veterinary Research
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    • v.38 no.1
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    • pp.139-145
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    • 1998
  • The Neospora sp. was isolated from the brain of 1 calf via continuous in vitro cultivation in Vero cell. Neospora tachyzoites were observed 45 days after inoculation of the homogenized brain suspension into the Vero cell. The isolated parasite (named tentatively as NCKB-1) was morphologically and ultrastructurally similar to the previously reported Neospora sp isolated in cattle (BPA-1, JPA-1). A comparison of the antigenic reactivity of cultivated tachyzoites with polyclonal antisera to Neospora caninum and Toxoplasma gondii confirmed that this protozoal isolate was similar to N caninum. This is the first report of successful isolation of Neospora sp from cattle in Korea.

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Repeated bovine abortion associated with Neospora caninum in Korea (Neospora caninum에 의한 젖소의 반복유산)

  • Kim, Jae-hoon;Hwang, Eui-kyung;Sohn, Hyun-joo;Jean, Young-hwa;Yoon, Soon-seek;Kim, Dae-yong
    • Korean Journal of Veterinary Research
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    • v.38 no.4
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    • pp.853-858
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    • 1998
  • A case of repeated bovine abortion caused by Neospora caninum is described. The 3-year-old Holstein cow, which was seropositive to N caninum antibody by indirect immunofluoresent assay, aborted consecutively within a 10-month period. Two fetuses of 6- or 5-month of gestation were aborted in June 1996 and March 1997, respectively. Histologically, multifocal necrotizing encephalitis, nonsuppurative periportal hepatitis, myocarditis, and myositis were observed in both fetuses. Tachyzoites or tissue cysts detected in the brain, heart and liver were positive to N caninum but negative to Toxoplasma gondii by immunohistochemical method. The results of our study demonstrate that repeated abortion due to N caninum actually occurs in Korea. This is believed to the first report of repeated abortion associated with N caninum in Korea.

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Seroprevalence of antibodies to Neospora caninum in Korean indigenous cattle in Jeongeup province (정읍지역에서 사육중인 한우에서 Neospora caninum 항체 양성율 조사)

  • Jeong Jae-Myong;Kweon Mi-Soon;Yoon Yeo-Baik;Han Kyu-Sam
    • Korean Journal of Veterinary Service
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    • v.28 no.2
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    • pp.99-106
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    • 2005
  • This survey was carried out to investigate the seroprevalence of antibodies to Neospora caninum in Korean indigenous cattle that was representative livestock raised in Jeongeup province Jeonbuk Korea. A total of 1,162 sera were tested for N caninum antibodies using ELISA (Herdcheck anti-Neospora. IDEXX Laboratories Inc., Westbrook, Maine USA). $15(1.3\%)$ sera were positive by ELISA. A total of 290 farms were tested. $11(3.8\%)$ farms were positive. Among the seroprevalence of cattle according to the areas, 8 of 44 counties were infected N caninum. Sero-positive 11 farms of N caninum antibodies using ELISA test and 62 of 301 sera $(20.6\%)$ were infected to N caninum. We found that cattle be raised in Jeongeup province was slightly infected to N caninum. It seems to be infected of N caninum an early stage. but the positive rates was variable that it was $6\%\~62\%$. It was supposed to the highest positive rate of 3 farms had some factors to be infected. The factors were supposed to keep dogs yearly, located the base of hills, and almost feed with imported dried grass. Farmers suppose to the cause of abortion was not the neosporosis but also broke out an accident or was infected Brucella spp. The results of Brucella Rose Bengal Test (RBT) were all negative.