• Title, Summary, Keyword: ODS cartridge

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Development of New Analysis Method of Cyanobacterial Toxins in Reservoirs (호수에서의 남조류 독성물질의 새로운 분석법 개발)

  • Pyo, Dong Jin;Song, Gi Seop;Yun, Seok Chang;Kim, Beom Cheol;Lee, Dae Un
    • Journal of the Korean Chemical Society
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    • v.38 no.10
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    • pp.741-748
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    • 1994
  • A new HPLC method for the analysis of cyanobacterial toxins, i.e. microcystin was developed using cyano-type prepacked cartridge while the conventional method was to utilize ODS cartridge. The cartridge was washed with 0.5 M acetic acid, then microcystins RR and LR were eluted from the cartridge with 30% acetonitrile. A better degree of quantitation was observed than with a ODS cartridge. Especially, in the case of microcystin LR a great difference in peak area was observed.

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Simultaneous detection of residual fungicide in Bean Sprouts by Solid-phase Extraction (고체상 추출법에 의한 콩나물 중의 잔류농약 동시 검출)

  • Lee, Myunghwoon;Lee, Taejoon;Pyo, Dongjin
    • Analytical Science and Technology
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    • v.6 no.5
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    • pp.509-514
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    • 1993
  • Benomyl, topsin-M and thiram been known as one of the seed disinfectant. For these studies benomyl, topsin-M and thiram simultaneously had been analyzed by high performance liquid chromatographic method using ODS cartridge, benomyl, topsin-M and thiram was chromatographed using an ODS column and eluent 40% Acetonitrile at a flow rate of 1ml/min. The UV Detector responses at 276nm.

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Study on New Extraction Method of Microcystins from Cyanobacteria (남조류로부터 마이크로시스틴을 추출하는 새로운 추출법 연구)

  • Pyo, Dong Jin;Shin, Hyun Du
    • Journal of the Korean Chemical Society
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    • v.45 no.2
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    • pp.149-155
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    • 2001
  • A new analytical method of cyanobacterial toxins, i, e, microcysins was deveeloped using supercritical fluid extraction(SFE). The microcystins mcluded in the study are sparsely soluble in neat supercritical fluid CO$_2$ However, the microcystins were successfully extracted with a temary mixture(90% CO$_2$,9.0% methanol 1.0% water) at 40$^{\circ}$C and 250 atm. The SFE method developed in this study has several advantages over solid-phase extraction(SPE) sample preparation for the analysis of microcystins. Sample handling steps are minimized thus reducing possible losses of analytes and saving analysis time. No clean-up steps are employed in this SFE method. Althouhgh many methods have been described for microcystim RR and LR, the method using solid-phase extraction with ODS cartridges is the most commonly used. However, the adsorbing power of ODS caridges for microcystins is weak, so we have attempted to use a more polar CN cartridge, to increase the adsorbing power for microcystins. Lyophilized cells(100mg) were wxtracted with 5% (v/v) acetic acid. The extract was centrifuged and then the supernatant was applied to a CN cartridge. The cartridge which contained microcystins was rinsed with 5 ml of water and 5 ml of 0.5 M acetic acid. followed by 5 ml of 5% acetonitrile in water , and were determined by HPLC. Better recoveries and chromatogram were observed than with ODS cartridge.

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Inhibitory Effect of Hexane Fraction from Myagropsis myagroides on Pancreatic α-Amylase In Vitro

  • Pak, Won-Min;Kim, Koth-Bong-Woo Ri;Kim, Min-ji;Cho, Ji-Young;Ahn, Dong-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.25 no.3
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    • pp.328-333
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    • 2015
  • A Myagropsis myagroides (Mm) methanol extract showed α-amylase inhibitory activity of 13% at a concentration of 5 mg/ml. Results showed that the hexane fraction from the Mm methanol extract exhibited α-amylase inhibitory activity with an IC50 value of 4.24 mg/ml. The hexane fraction was separated using silica-gel column chromatography, and six subfractions were obtained. The fraction eluted with CHCl3:MeOH = 50:1 showed the highest α-amylase inhibitory activity with an IC50 value of 0.72 mg/ml. This fraction was purified using Sephadex LH-20 column chromatography and an octadecyl silica (ODS) Sepak cartridge, obtaining seven subfractions. Fraction (Fr.) 4 also showed a strong α-amylase inhibitory activity with an IC50 value of 0.75 mg/ml. Fr. 4 was purified by Sephadex LH-20 column chromatography and ODS Sepak cartridge, obtaining six subfractions. Fr. 4-2 was identified as sargachromanol I with an IC50 value of 0.40 mg/ml, and the inhibition pattern analyzed from Lineweaver-Burk plots revealed it to be an uncompetitive inhibitor. These results suggest that Mm has potential as a natural antidiabetes agent.

Determination of Patulin in Commercial Apple Juice in Korea by High Performance Liquid Chromatography (High Performance Liquid Chromatography를 이용한 국내 시판 사과 주스의 Patulin 분석)

  • Cho, Wan-Il;Choi, Young-Boong;Moon, Tae-Wha
    • Korean Journal of Food Science and Technology
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    • v.29 no.3
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    • pp.412-416
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    • 1997
  • Seven brands of commercial apple juice in Korea obtained from local stores were analyzed for patulin. Sep-Pak silica cartridge purification of the extract by ethyl acetate offered a good separation of patulin from other components. Patulin was determined by reverse phase liquid chromatography using a J'sphere $4\;{\mu}m$ ODS-H80 column with an ultraviolet detector set at 274 nm. Patulin concentrations in four samples ranged from 4 to $20\;{\mu}g/L$ and the other three samples from 30 to $45\;{\mu}g/L$. The limit of detection was $3\;{\mu}g/L$, and the recovery was $80{\sim}90%$ at the contamination level of $3.78{\sim}125.9\;{\mu}g/L$.

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Evaluation of Pre-treatment Stages for Microcystins Analysis using LC/MS/MS (LC/MS/MS 분석을 위한 microcystins 전처리 단계별 효율성 연구)

  • Kim, Hwa-Bin;Park, Hae-Kyung;Moon, Jeong-Suk
    • Journal of Korean Society on Water Environment
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    • v.25 no.5
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    • pp.720-726
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    • 2009
  • We investigated the most effective pre-treatment processes and LC/MS/MS condition for microcystins analysis. With a step-by-step pre-treatment, efficiencies of several established methods were compared. At the level of cell burst, sonication method was found to be the most efficient. As a mycrocystins first extraction solvent, 5% acetic acid showed the highest efficiency. An isolation and recovery rate of mycrocystins of ODS Sep-Pak $C_{18}$ cartridge was higher than HLB SPE cartridge. As a final elution solvent from cartridge, 100% MeOH had a better efficiency than others. Using a LC/MS/MS, effective analytical methods were established. C18 reverse column was used and gradient elution was performed with using acetonitrile, 0.1% formic acid as a mobile phase. We analysed to 0.8 mL/min flow rate fit to the $5{\mu}m$ particle size column and $55^{\circ}C$ housing temperature. The validity of established analytical method was evaluated that MDL as average $0.050{\pm}0.014{\mu}g/L$ and LOQ as average $0.160{\pm}0.045{\mu}g/L$ had a good sensitivity over 40 magnification rather than $2{\mu}g/L$ detection limit of HPLC.

Multisample Extraction system for Solid Phase Extraction of Dissolved Organic Compounds from Sea Water (해수로부터 용존 윤기물의 Solid Phase Extraction을 위한 다수 시료 처리 장치)

  • Cho Ki Woong;Jung Kyungwha;Shin Jongheon;Kim Suk Hyun;Hong Gi-Hoon
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.3 no.3
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    • pp.34-40
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    • 2000
  • A multisample extraction device was newly designed for efficient extraction of dissolved lipophillic organic compounds from sea water sample. This device allowed extraction of organic compounds from up to 96 sample at a time using 96 multifolder on the principle of solid phase extraction with commercially available octadecyl silane (ODS) cartridges. The recovery yield of the new divice was higher than 90 % while that of conventional liquid-liquid extraction process are only 60 - 70 %. The amount of solvent required for the new device could be reduced to less than 20㎖ per 1ℓ of sample while 1 - 2 ℓ of solvent were used in the conventional liquid-liquid extraction process. The usefulness of this novel method was demonstrated with sea water samples collected from Yellow sea, and the qualitative and quantitative analyses results of the dissolved hydrocarbon showed this method was superior to that of conventional liquid-liquid extraction process in efficiency and reliability.

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