• Title, Summary, Keyword: PA-TCH-SP-PD

Search Result 2, Processing Time 0.038 seconds

Application of Periodic Acid Thiocarbohydrazide Silver Proteinate Physical Development ( PA-TCH-SP-PD) Stain to Observation of Sertoli Cell (세르톨리세포 관찰을 위한 PA-TCH-SP-PD 염색의 적용)

  • 박영석;이성호
    • Korean Journal of Animal Reproduction
    • /
    • v.22 no.4
    • /
    • pp.331-339
    • /
    • 1998
  • The purpose of this study was to investigate the applicability of periodic acid thiocarbohy-drazide silver proteinate, physical development (PA-TCH-SP, -PD) stain to the seminiferous tubules for the ultrastructural studies of Sertoli cell column and Sertoli cell processes. In the Sertoli cell cloumn and Sertoli cell processes, high concentration of the reactive granules were observed under transmission electronmicroscope (TEM) after PA-TCH-SP-PD stain. Also some reactive granules were seen in the spermatogonium cytoplasm, clearly. These reactive granules specifically stained with PA-TCH-SP, -PD make the Sertoli cell column, Sertoli cell processes and spermatogonium cytoplasm easy to distinguish from nucleus of the germ cells, spermatocyte, spermatid and residual body which did not contain the reactive granules. This result indicates that the PA-TCH-SP, -PD stain is superior to other traditional electronic double stain methods for the ultrastructural studies of Sertoli cell.

  • PDF

Glycogen distribution of germ cells and Sertoli cells of seminiferous tubules in Jindo dog (진도견 정세관의 정세포와 Sertoli 세포내 glycogen의 분포)

  • Park, Young-seok;Lee, Seong-ho
    • Korean Journal of Veterinary Research
    • /
    • v.36 no.3
    • /
    • pp.521-529
    • /
    • 1996
  • In an effort to obtain basic data of carbohydrate metabolism during spermiogenesis of the sexually-matured Jindo dog, the glycogen distribution in the testis was investigated by light and transmission electron microscopy. Periodic acid thiocarbohydrazide silver proteinate physical development(PA-TCH-SP-PD) staining method provided better results in the detection of glycogen granules from Sertoli cells and germ cells than the periodic acid schiff(PAS) staining method did. Pre-treatment of the tissue sections with ${\alpha}$-amylase elicited a significant decrease in PA-TCH-SP-PD stained granules, which suggested that the stained granules were of glycogen origin. High concentration of the glycogen granules were observed in the Sertoli cells, especially in its column, sheet-like processes, club-like processes, and tubular processes. The glycogen granules were unevenly distributed in some Sertoli cell columns. These results strongly indicated that the Sertoli cells of Jindo dogs showed vigorous activity of carbohydrate metabolism.

  • PDF