• Title/Summary/Keyword: PEG

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PEGYLATION: Novel Technology to Enhance Therapeutic Efficacy of Proteins and Peptides (PEG 접합: 단백질 및 펩타이드 치료제의 약효를 증가시키는 새로운 기술)

  • Park, Myung-Ok;Lee, Kang-Choon
    • Journal of Pharmaceutical Investigation
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    • v.30 no.2
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    • pp.73-83
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    • 2000
  • Polyethylene glycol (PEG) is a water soluble, biocompatible, non-toxic polymer and PEGylation is a well established technique for the modification of therapeutic proteins and peptides. PEG-protein drugs have been extensively studies in relation to therapies for various diseases: cancer, inflammation and others. The covalent attachment of PEG to proteins and peptides prolonged plasma half-life, reduced antigenicity and immunogenicity, increased thermal and mechanical stability, and prevented degradation by enzymes. Several chemical groups for general and site specific conjugation have been exploited to activate PEG for amino group, carboxyl group, and cysteine groups. PEGylation of many proteins and peptides have been studied to enhance their properties for the potential uses. Also, the different positional isomers in several PEG-proteins have shown the difference in vivo stability and biological indicating that the site of PEG molecule attachment is one of the important factor to develop PEG-proteins as potential therapeutic agents.

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Characterization of patterned biochip fabricated by using photolithographic method of plasma polymerized polyethylene glycol

  • Choe, Chang-Rok;Choe, Geon-O;Jeong, Dong-Geun;Mun, Dae-Won;Lee, Tae-Geol
    • Proceedings of the Korean Vacuum Society Conference
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    • pp.399-399
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    • 2010
  • Polyethylene glycol(PEG)은 강력한 단백질 및 세포흡착 억제력을 가지고 있어 다양한 생물학적 연구에 사용되고 있으나, 기판과의 결합력이 무척 약해 기판 위에 박막을 형성하기가 매우 어렵다는 문제점이 있다. 이번 연구에서는 capacitively-coupled plasma chemical vapor deposition(CCP-CVD)를 이용하여 PEG를 유리 기판 위에 플라즈마 중합하여 plasma-polymerized PEG(PP-PEG) 기판을 만들었다. PP-PEG 박막은 FT-IR, XPS, ToF-SIMS 분석을 통하여 PEG와 매우 유사한 화학적 조성을 가지고 있음을 확인할 수 있었다. 또한 PP-PEG 기판은 photolithography 방법을 이용하여 표면에 photoresist를 패턴한 뒤 아민작용기를 가지는 plasma-polymerized ethylenediamine (PPEDA)를 증착하여 표면이 amine/PEG로 패턴화된 박막 기판을 만들었다. 패턴된 기판에 단백질 및 세포를 고정화하였을 때, 아민 작용기가 노출된 부분에만 고정화가 나타나고 PP-PEG 영역에는 단백질 및 세포의 흡착이 효율적으로 억제되는 것을 형광측정 및 ToF-SIMS chemical imaging 방법을 이용하여 확인하였다. 이러한 바이오칩 제작기술은 단백질 및 세포 칩을 포함한 여러 분야에서 폭넓게 응용될 수 있을 것으로 기대된다.

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Polyethylene glycol (PEG)-modified cationic liposome mediated gene delivery

  • Kim, Jin-Ki;Choi, Sung-Hee;Ahn, Woong-Shick;Kim, Chong-Kook
    • Proceedings of the PSK Conference
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    • pp.426.1-426
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    • 2002
  • In this study. we modified the cationic liposomes by polyethylene glycol (PEG)-grafted or PEG-added methods. The PEG-grafted transfection complexes were prepared by adding the plasmid DNA to the PEG-grafted cationic liposomes, composed of PEG and cationic lipids. PEG-added transfection complexes were prepared by adding the PEG to the mixture of cationic lipids and plasmid DNA. The particle sizes of PEG-modified transfection complexes did not change during storage compared to conventional transfection complexes. (omitted)

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2D Correlation Analysis of Spin-Coated Films of Biodegradable P(HB-co-HHx)/PEG Blends

  • Kim, Min-Kyung;Ryu, Soo-Ryeon;Noda, Isao;Jung, Young-Mee
    • Bulletin of the Korean Chemical Society
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    • v.32 no.11
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    • pp.4005-4010
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    • 2011
  • We investigated thermal behavior of spin-coated films of P(HB-co-HHx)/PEG blends by using infraredreflection absorption (IRRAS) spectroscopy and 2D correlation spectroscopy. Based on 2D IRRAS correlation spectra, we could determine the sequence of spectral intensity changes with increasing temperature that PEG band changes first and then a band for crystalline component of P(HB-co-HHx) changes before a band for amorphous component. The intensities of bands for PEG and amorphous P(HB-co-HHx) were changed greatly as PEG weigh % of P(HB-co-HHx)/PEG blends increased. Transition temperatures of P(HB-co-HHx)/PEG blends were successfully determined by 2D gradient mapping method. The transition temperature of spincoated films of 98/2 and 90/10 P(HB-co-HHx)/PEG blends and 80/20 P(HB-co-HHx)/PEG blend determined by 2D gradient map are, respectively, about 137.5 and $132.5^{\circ}C$. Furthermore, P(HB-co-HHx)/PEG blends show an additional transition temperature that have been interpreted in terms of different lamellar thicknesses in spin coated films.

Study on protein adsorption resistant soft contact lens (단백질흡착을 막는 소프트콘택트렌즈에 관한 연구)

  • Jeong, Young-Il;Cho, Chong-Su;Na, Jae-Woon;Kim, Sung-Ho
    • Proceedings of the KOSOMBE Conference
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    • v.1996 no.05
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    • pp.223-225
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    • 1996
  • Poly (ethylene glycol) (PEG)macromers terminated with acrylate groups and interpenetrating polymer networks(IPN) composed of poly(hydroxyethyl methacrylate)(PHEMA) or poly(hydroxyethyl methacrylate-co-hydropropyl methacrylate-co-N-vinyl pyrrolidone) [P(HEMA-co-HPMA-co-NVP)] and PEG macromer were synthesized with the aim of obtaining protein adsorption resisatnt soft contact lens. Polymerization of PEG macromer resulted in the formation of cross-linked gels due to the multifunctionality of macromer. Crosslinked P(HEMA) or P(HEMA-co-HPMA-co-NVP) chains were interpenetrated into the cross-linked three-dimensional networks of PEG. It was found that albumin adsorption onto the contact lens prepared by P(HWMA)/PEG IPN decreases with a decrease of molecular weight of PEG whereas its adsorption onto the contact lens prepared by P(HEMA-co-HPMA-co-NVP)/PEG IPN decreases with an increase of molecular weight of PEG. Also, it was found that albumin adsorption onto the both contact lens decreases with an increase of concentration of PEG macromer in the IPN preparation. There are also more adequate in the bioinertness and bioadhesion for the contact lens by P(HEMA)/PEG IPN or P(HEMA-co-HPMA-co-NVP)/PEG IPN than that by P(HEMA) or P(HEMA-co-HPMA-co-NVP).

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Preparation of PLA/PEG Block Copolymer via Melt Blend (용융블렌드를 이용한 PLA/PEG 블록 공중합체의 제조)

  • Yoon, Cheol-Soo;Ji, Dong-Sun
    • Textile Science and Engineering
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    • v.43 no.5
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    • pp.235-244
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    • 2006
  • PLA/PEG blends composed of poly(lactic acid) (PLA) and poly (ethylene glycol) (PEG) were prepared via melt blending for bioabsorbable filament sutures. The effects of blend composition and blending time on the alcoholysis or esterification in the PLA/PEG blends were investigated. Their properties due to PLA/PEG block copolymer were characterized by $^1$H-NMR, GPC, and DSC analyses. Consequently, PLA/PEG block copolymers were formed during the melt blending by alcoholysis or esterification from a new peak due to -CH$_2$(c') appeared at 4.3 ppm. In addition, the molecular weights of the blends processed in Haake Rheomix for 10$\sim$30 minutes at 220 $^{\circ}C$ under screw speed 20 rpm decreased with increasing PEG contents and blending time. Their thermal properties such as melting temperature and heat of fusion due to PLA in the blends decreased with increasing PEG contents and blending time. However, their melting temperature and heat of fusion due to PEG increased with an increase of PEG content under constant blending time.

Partition Coefficient of Proteins of Different Surface Hydrophobicity in Poly (ethylene glycol)-Dextran Aqueous Two Phase System (Poly(ethylene glycol)-Dextran 수용액 2상계에서 단백질들의 소수성에 따른 분획계수)

  • Lee, Sam-Pin;Lee, Cherl-Ho
    • Korean Journal of Food Science and Technology
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    • v.19 no.2
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    • pp.140-145
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    • 1987
  • The partition coefficient of the proteins of known effective hydrophobicity was determined in a poly (ethylene glycol)-dextran aqueous two-phase system. The changes in the partition coefficient was also determined when a fraction of PEG-palmitate (PEG-P) was added to the system. The partition coefficient of the proteins increased as the concentrations of PEG and dextran increased at a constant phase volume ration irrespective of the effective hydrophobicity of the proteins. When small amounts of PEG-P were added to the PEG phase, the partition coefficients of BSA and ${\beta}-lactoglobulin$, which had relative hydrophobicity (RI) of 700 and 120, respectively, increased more than ten-fold, whereas ovalbumin whose RI was 5 showed little change. The drastic increases m the partition coefficient were observed by the addition of PEG-P in 2% level to the PEG system. Addition of PEG-P over 5% level resulted in a slight further increase in the partition coefficient of all proteins tested.

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Compressive Strength of Waterlogged Archaeological Wood after PEG Treatment with Concentration and Solvent (PEG 처리 수침고목재의 농도 및 용매에 따른 압축강도 변화)

  • Kim, Soo-Chul
    • Journal of Conservation Science
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    • v.28 no.2
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    • pp.95-99
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    • 2012
  • The compressive strength of PEG along processing concentration and solvent is willing to be measured and proper processing condition for exhibition and storage is also willing to be measured by comparing with dimensional stability. In the advanced research of setting PEG-preprocessing concentration & solvent for freeze drying of waterlogged archaeological wood of high water content, vacuum freeze drying showed the highest dimension stability after 40% PEG-preprocessing of aqueous solution. In this study, the compressive strength increased in proportion of processing concentration and water showed the relatively-higher compressive strength than t-butanol regarding solvent. Especially, it showed that there is no big strength difference between PEG 40% and PEG 50% in aqueous solution by 6.6%(16kgf/$cm^2$). According to the above results, it was recognized that it is most effective to implement freeze drying after 40% PEG-preprocessing when want to dimensional stability and compressive strength simultaneously.

Synthesis of Ion Conducting Polymer Having Low Temperature Characteristics : I. Synthesis and Characterization of Amorphous PEO Copolymer (저온특성을 갖는 이온전도성 고분자의 합성 연구 : I. 비정형 PEO 공중합체의 합성 및 분석)

  • 황승식;조창기
    • Polymer(Korea)
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    • v.24 no.1
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    • pp.133-139
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    • 2000
  • Poly(ethylene glycol) with number-average molecular weight (M$_{n}$) of 200 (PEG 200) or 400 (PEG 400) was reacted with various linking agents (CH$_2$Cl$_2$, CH$_2$Br$_2$, CH$_2$I$_2$, Br(CH$_2$)$_3$Br) in the presence of alkali to form of oxyalkylene linked chains. Molecular weights of copolymers were controlled using feed mole ratio of alkali/CH$_2$C1$_2$/PEG. The M$_{n}$ of the polymers measured by end group analysis and that measured by GPC agreed well. Molecuglar weights of polyether copolymers obtained from PEG 200 and PEG 400 were about 500~8500 and 1000~2000, respectively. Polyether copolymers prepared from PEG 400 showed melting points of around 1$0^{\circ}C$. Glass transition temperatures of the copolymers were around -75$^{\circ}C$ and the crystallinity was about 0~25%. The polyether copolymers prepared from PEG 200 had no crystallinity below the M$_{n}$ of 2500. 2500.

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Studies on the Dimensional Stabilization of Wood -Especially by the Solution of Different Composition by PEG Molecular Weight- (목재(木材)의 치수 안정화(安定化)에 관(關)한 연구(硏究) -Polyethylene Glycol의 혼합액(混合液)을 중심(中心)으로-)

  • Oh, Joung Soo;Cheon, Cheol
    • Journal of Korean Society of Forest Science
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    • v.76 no.2
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    • pp.119-126
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    • 1987
  • This study was carried out to elucidate the desirable PEG molecular weight and it's blending methods which can promote the dimensional stabilization of Corpus controversa, Quercus variabilis and Prunus sargentii. The results may be summarized as follows: 1. PEG polymer loading in woods was affected by specific gravity of wood. And the PEG polymer loading was satisfactory except in Quercus variabilis, which shows the blending method of PEG V was proper. 2. Bulking coefficiency of PEG V was high in Corpus controversa and Prunus sargentii, and it is necessary to paint or coat water-proofing wood preservative: for high bulking coefficiency of Quercus variabilis. 3. In the treatment of PEG V, the antishrink efficiency of Prunus sargentii was 82.59% and that of Quercus variabilis 62.79%. 4. Dimensional stabilization of hardwoods did not have apparant relation with PEG blending method. 5. Judging from results, PEG-400 and PEG-1000 would be enough for dimensional stabilization of hardwood having high specific gravity if other factors are considered well.

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