• Title, Summary, Keyword: PTS

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Cloning and Characterization of the Lactococcus lactis subsp. lactis ATCC 7962 pts HI Operon

  • Kim, Tea-Youn;Park, Rae-Jun;Chang, Hae-Choon;Chung, Dae-Kyun;Lee, Jong-Hoon;Lee, Hyong-Joo;Kim, Jeong-Hwan
    • Journal of Microbiology and Biotechnology
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    • v.10 no.6
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    • pp.829-835
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    • 2000
  • The ptsH and ptsI genes of Lactococus lactis subsp. lactis ATCC 7962 (L. lactis 7962), encoding the general proteins of phosphotransferase system (PTS) components, HPr and enzyme I, respectively, were cloned and characterized. A 1.3 kb PCR product was obtained using a primer set that was hybridized to the internal region of the L. lactis 7962 pts HI genes and then subcloned into a low-copy number vector, pACYC184. The 5' upstream and 3' downstream region from the 1.3 kb fragment were subsequently clone using the chromosome walking method. The complete ptsHI operon was constructed and the nucleotide sequences determined. Two ORFs corresponding to HPr (88 amino acids) and enzyme I (575 amino acids) were located. The ptsHI genes of L. lactis 7962 showed a very high homology (84-90%) with those genes from other Gram-positive bacteria. A primer extension analysis showed that the transcription started at either one of two adjacent bases upstream of the start codon. Using a Northern analysis, two transcripts were detected; the first, a 0.3 kb transcript corresponding to ptsH and the second, a 2 kb transcript corresponding to ptsH and ptsI. The transcription level of ptsH was higher than that of ptsI. The concentration of the ptsH transcript in cells grown on glucose was similar to that in cells grown on lactose, yet higher than that in cells grown on galactose. The ptsI transcript was scarcely detected in cell grown on lactose or galactose. The ptsI transcript was scarcely detected in cells grown on lactose or galactose. The results of a sequence analysis and Northern blot confirmed that the ptsH and ptsI genes of L. lactis 7962 were arranged in an operon like other known ptsHI genes and the expression of the ptsHI genes was regulated at the transcriptional level in response to the carbon source.

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Surgical Correction of Tetralogy of Fallot in Adults over 20 Years of Age (성인 20 세 이상 활로 4징증의 수술성적)

  • 정윤섭
    • The Korean Journal of Thoracic and Cardiovascular Surgery
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    • v.23 no.2
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    • pp.253-259
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    • 1990
  • Between January, 1970 and August, 1989, a total of 81 patients whose age were more than 20 years of life, received total correction for tetralogy of Fallot. This report analyzed 70 patients among them and excluded the remaining 11 patients whose clinical data could not be found. Their mean age was 25.750.39 years[range 20 \ulcorner50]. The clinical manifestations were cyanosis and clubbing [64 pts], frequent URI[40 pts], anoxic spell [19 pts], infective endo-carditis[4 pts], brain abscess[3 pts], pulmonary tuberculosis[3 pts] and CHF, chest tightness, nephrotic syndrome, left hemiplegia, and tamponade. The types of right ventricular outflow tract obstruction were combined[46 pts], pure infundibular [21 pts] and pure valvular[3 pts]. Associated cardiovascular anomalies were PFO [27 pts], ASDi8 pts], LSVC[8 pts], aortic regurgitation [5 pts], right aortic arch, coronary artery anomalies, PDA and dextrocardia. Hospital mortality was 5.7%. The causes of death ware low cardiac output [2 pts], aggravation of CRF[1 pts] and brain damage[1 pts]. There was one late death because of residual intracardiac shunt and congestive heart failure. During the follow-up period, 16 patients were lost and the remaining 49 patients were asymptomatic and leading normal lives. Residual intracardiac shunt was detected in 5 patients with radionuclide single pass study but all of them had Qp / Qs ratio less than 1.5.

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Analysis of Atopic Dermatitis Patients according to the Sasang Constitution (아토피 피부염 환자의 체질분석)

  • Kim Hae-jeong
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.16 no.3
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    • pp.200-209
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    • 2003
  • Objectives : The aim of this study was to investigate the relationship between atopic dermatitis and the Sasang constitution. Methods : I examined 64 patients with moderate to severe atopic dermatitis for a long time who visited Department of Dermatology in Kangnam Korean Hospital, Kyunghee University and classified them into 4 types according to Sasang constitution under consulting a Sasang specialist. 1. The Sasang specialist classified the patients using the Questionnaire for the Sasang Constitution Classification Ⅱ(QSCCⅡ), body components examination, facial morphology measurement and Meridian function examination. 2. I analyzed the distribution of sex and age, first onset age, history period, digestion condition, existences of past history and family history, basal material volume, immunoglobulin E and eosinophil. Results and Conclusions : 1. As the distribution of sex and age, 28 patients(pts) were male and 36 pts were female. 20's female group was the most common. 2. As the distribution of Sasang constitution types, Soeumin were 28(43.7$\%$) pts, Taeumin were 24(37.5$\%$) pts, Soyangin were 11(17.2$\%$) pts, and Taeyangin was 1(1.6$\%$) pI. 3. As the mean age at first visit, Soeumin's was 20yrs, Taeumin's and Soyangin's were 27yrs, and Taeyangin's was 15yrs. 4. As the mean onset age, Soeumin's was 7yrs the lowest, Taeumin's was 15.7yrs, Soyangin's was 10.54yrs and Taeyangin's was 15yrs. 5. As the history period. Soeumin's was 13.8yrs. Taeumin's was 11.8yrs. Soyangin's was 16.5yrs. Tae yangin's was 13yrs. 6. As the existence of past history of atopic diseases, Soeumin were 12(43$\%$) pts, Taeumin were 7(29$\%$) pts and Soyangin were 5(45$\%$) pts. 7. As immunoglobulin E, 21(75$\%$) pts of Soeumin were normal, 7(25$\%$) pts were abnormal.. 22(92$\%$) pts of Taeumin were normal, 2(8$\%$) pts were abnormal. 8(73$\%$) pts of Soyangin were normal, 3(27$\%$) pts were abnormal. As the defecation condition, 19(68$\%$) pts of Soeumin were normal, 9(32$\%$) pts were abnormal. 14(58$\%$) pts of Taeumin were normal, 10(42$\%$) pts were abnormal, 8(73$\%$) pts of Soyangin were normal, 3(27$\%$) pts were abnormal. 8. The mean value of immunoglobulin E of Soeumin was 1236, Taeeumin was 442, Soyangin was 2010, the mean value of eosinophil of Soeumin was 3.5, Taeeumin was 2.47, Soyangin was 4.28.

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A Study on PAPR Characteristic using High-speed adaptation PTS Method in OFDM System (OFDM 시스템에서 고속 적응형 PTS 기법을 이용한 PAPR 특성에 대한 연구)

  • Sung Tae-Kyung;Kim Dong-Seek;Cho Hyung-Rae
    • Proceedings of the Korea Contents Association Conference
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    • pp.458-463
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    • 2005
  • OFDM communication system is effective for the high data rate transmission in the frequency selective fading channel. Since OFDM has high PAPR, OFDM signal may be distorted by the nonlinear HPA. In this paper, we propose an combined SLM and PTS method for reducing the PAPR in OFDM communication system. Proposed method increased some system complex in comparison with exiting method. But we made sure of high efficiency in the case of reducing the PAPR. As a result of using the 16-QAM modulation and increasing the carrier number in the SLM, PTS method and the SLM-PTS combined method, SLM-PTS combined method is more an effective method of reducing 3.5 dB PAPR than exiting OFDM system when this method is M, L=3, D=128, 256 in 10-3 BER.

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Filamentous growth of Escherichia coli by dephosphorylated NPr (탈인산화된 NPr에 의한 대장균의 섬유상 생장)

  • Choi, Umji;Seok, Yeong-Jae;Lee, Chang-Ro
    • Korean Journal of Microbiology
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    • v.53 no.3
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    • pp.149-155
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    • 2017
  • The nitrogen phosphotransferase (PTS) system is a regulatory cascade present in most Proteobacteria, where it controls different functions. The nitrogen PTS is usually composed of $EI^{Ntr}$ (encoded by the ptsP gene), NPr (encoded by the ptsO gene), and $EIIA^{Ntr}$ (encoded by the ptsN gene). While $EIIA^{Ntr}$ plays a role in a variety of cellular processes, such as potassium homeostasis, regulation of ppGpp accumulation, nitrogen and carbon metabolisms, and regulation of ABC transporters, little information is available for a physiological role of NPr. A recent study showed that dephosphorylated NPr affects adaptation to envelope stresses in Escherichia coli. In this study, we provide another phenotype related to NPr. The ptsP mutant showed a filamentation phenotype. The filamentation phenotype of the ptsP mutant was recovered by additional deletion of the ptsO gene, but not by additional deletion of the ptsN gene, suggesting that an increased level of dephosphorylated NPr in the ptsP mutant renders cells the filamentous growth. This idea was confirmed by the fact that cells with increased levels of dephosphorylated NPr shows the filamentation phenotype. Additionally, we showed that cell size of E. coli increases with incremental dephosphorylated NPr concentrations. These results suggested that dephosphorylated NPr induces morphological change of E. coli.

Mechanism of Regulation of the pts Promoter Transcription Initiation by Carbon Sources in Escherichia coli (Carbon Source의 변화에 의한 대장균의 pts Promoter 전사 조절 기작)

  • Kim, Soon-Young;Kwon, Hyuk-Ran;Shin, Dong-Woo;Ryu, Sang-Ryeol
    • Applied Biological Chemistry
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    • v.42 no.4
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    • pp.293-297
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    • 1999
  • The pts operon, which encodes several factors in the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) of Escherichia coli, has multiple promoters which respond to different signals to facilitate quick adaptation to changes in growth conditions. The influence of an 1 kbp DNA region upstream of the pts P0 promoter on pts expression was studied in vitro by employing the DNA templates containing both P0 and P1 promoter with or without the 1 kbp upstream DNA region for in vitro transcription assay. The 1 kbp DNA region upstream of the pts P0 promoter, however, had no effect on pts transcription in vitro. The intracellular concentration of cAMP was measured when cells were grown in the presence of glucose, mannose, or mannitol. The transcription of P0 was increased maximally in the presence of glucose even though the concentration of cAMP in the condition was lowest while the transcription from the P1b was highest when cells were grown in the presence of mannose or mannitol even though the intracellular concentration of cAMP was lower than cells grown in the absence of the sugar. These results suggest the possibility of the existence of a glucose inducible repressor specific for the P0 promoter and a second repressor that is inducible by glucose, mannose and mannitol specific for the P1 promoter.

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PTS Technique Based on Micro-Genetic Algorithm with Low Computational Complexity (낮은 계산 복잡도를 갖는 마이크로 유전자 알고리즘 기반의 PTS 기법)

  • Kong, Min-Han;Song, Moon-Kyou
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.33 no.6C
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    • pp.480-486
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    • 2008
  • The high peak-to-average power ratio (PAPR) of the transmitted signals is one of major drawbacks of the orthogonal frequency division multiplexing (OFDM). A partial transmit sequences (PTS) technique can improve the PAPR statistics of OFDM signals. However, in a PTS technique, the search complexity to select phase weighting factors increases exponentially with the number of sub-blocks. In this paper, a PTS technique with low computational complexity is presented, which adopts micro-genetic algorithm(${\mu}$-GA) as a search algorithm. A search on the phase weighting factors starts with a population of five randomly generated individuals. An elite having the largest fitness value and the other four individuals selected through the tournament selection strategy are determined, and then the next generation members are generated through the crossover operations among those. If the new generation converges, all the four individuals except the elite are randomly generated again. The search terminates when there has been no improvements on the PAPR during the predefined number of generations, or the maximum number of generations has been reached. To evaluate the performance of the proposed PTS technique, the complementary cumulative distribution functions (CCDF) of the PAPR are compared with those of the conventional PTS techniques.

Multi-carriers PAPR Reduction Method using Adaptive Sub-optimal PTS with Threshold (다중반송파 PAPR 감소를 위한 임계치 적용 적응 부최적 PTS 기법 연구)

  • 권오주;하영호
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.26 no.12A
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    • pp.2012-2018
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    • 2001
  • This paper proposes the adaptive suboptimal iterative algorithm using threshold to reduce system complexity in the PTS\`s. Performance of the proposed adaptive suboptimal iteration algorithm is represented in terms of iteration number and CDF. In the case of the number of sub-block is 4, the 10-3 PAPR of the proposed method and P S improved this by 0.4dB compared to Cimini\`s. And the complexity of the proposed method was reduced to nearly 22% for the PTS\`s and 44% for the Cimini\`s for 8dB threshold. For the 8 sub-blocks, the 10$\^$-3/ PAPR of the proposed method reduced by 0.7dB compared to PTS\`s, but improved by 0.4dB compared to Cimini\`s. And the complexity of the proposed method was reduced to nearly 2.4% for the PTS\`s and 39% for the Cimini\`s.

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Effect of Polytriazolesulfone Addition on Fracture Toughness of DGEBA Epoxy Resin (DGEBA에 대한 폴리트리아졸술폰의 강인화 효과 연구)

  • Kwon, Woong;Lee, Minkyu;Han, Minwoo;Jeong, Euigyung
    • Textile Coloration and Finishing
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    • v.31 no.2
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    • pp.118-126
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    • 2019
  • This study aims to investigate the effect of polytriazolesulfone(PTS) addition on fracture toughness of diglycidyl ether of bisphenol A(DGEBA) and 4,4'-diaminodiphenylsulfone(DDS). Various amounts of PTS were added to DGEBA/4,4'-DDS in diazide and dialkyne monomer forms and polymerized during the epoxy curing process. Fracture toughness(K1C), tensile properties and thermal stability of the PTS added epoxy resin were evaluated and compared with those of PES, the conventional high Tg toughening agent, added epoxy resin. Fracture toughness of the PTS added epoxy resin was dramatically improved up to 133%, as the amount of PTS added increased, whereas that of the PES added epoxy resin was improved by only 67%. The tensile strength of PTS added DGEBA/4,4'-DDS was similar to the epoxy resin without PTS and tensile modulus was improved by 20%. And thermal stability of the PTS added epoxy resin was improved up to 14%. Therefore, PTS addition to DGEBA/4,4'-DDS, as a toughening agent, is very effective way to improve its fracture toughness without any lowering in other properties.

New low-complexity segmentation scheme for the partial transmit sequence technique for reducing the high PAPR value in OFDM systems

  • Jawhar, Yasir Amer;Ramli, Khairun Nidzam;Taher, Montadar Abas;Shah, Nor Shahida Mohd;Audah, Lukman;Ahmed, Mustafa Sami;Abbas, Thamer
    • ETRI Journal
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    • v.40 no.6
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    • pp.699-713
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    • 2018
  • Orthogonal frequency division multiplexing (OFDM) has been the overwhelmingly prevalent choice for high-data-rate systems due to its superior advantages compared with other modulation techniques. In contrast, a high peak-to-average-power ratio (PAPR) is considered the fundamental obstacle in OFDM systems since it drives the system to suffer from in-band distortion and out-of-band radiation. The partial transmit sequence (PTS) technique is viewed as one of several strategies that have been suggested to diminish the high PAPR trend. The PTS relies upon dividing an input data sequence into a number of subblocks. Hence, three common types of the subblock segmentation methods have been adopted - interleaving (IL-PTS), adjacent (Ad-PTS), and pseudorandom (PR-PTS). In this study, a new type of subblock division scheme is proposed to improve the PAPR reduction capacity with a low computational complexity. The results indicate that the proposed scheme can enhance the PAPR reduction performance better than the IL-PTS and Ad-PTS schemes. Additionally, the computational complexity of the proposed scheme is lower than that of the PR-PTS and Ad-PTS schemes.