• Title, Summary, Keyword: Petasites japonicus

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A study on the chemical and dyeing properties of Petasites japonicus leaf extract (머위잎 추출액의 염색성)

  • 김애순
    • Journal of the Korean Society of Clothing and Textiles
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    • v.28 no.3_4
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    • pp.444-451
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    • 2004
  • This study was carried out to investigate the chemical and dyeing properties of Petasites japonicus leaf extract under the various extracting and dyeing conditions such as temperature, time, the ratio of water and pH, repetition of dyeing in silk and cotton fabrics with Petasites japonicus leaf. The results were as follows: 1. It was found that λ$_{\max}$ of color solution extracted by Petasites japonicus leaf has two peaks at 290nm and 323nm. 2. The optimum extracting conditions were studied at 10$0^{\circ}C$, 40min., pH 7 and 1 : 20(the ratio of water and Petasites japonicus leaf), the optimum dyeing temperature, dyeing time, dyeing pH and repetition of dyeing were 10$0^{\circ}C$, 60min., pH 7, repetitions of three times, respectively. 3. Silk and cotton fabrics dyed with Petasites japonicus leaf extract were colored yellowish orange. The colory Petasites japonicus leaf extract in silk and cotton fabrics were deeped by same-mordanting with aluminum potassium sufate and cupric sulfate. 4. Washing fastness of silk fabrics was 4∼5 grade, but cotton fabrics was 3∼4 grade, so washing fastness of silk fabrics washed with neutral detergent was excellent.

Pathological changes on rats and mice fed with Petasites japonicus Maxim I. Macroscopical and histopathological observations (머위(Petasites japonicus maxim)를 급여한 rat와 mouse에 대한 병리학적 관찰 I. 육안적 및 병리조직학적 관찰)

  • Jee, Young-heun;Lee, Cha-soo
    • Korean Journal of Veterinary Research
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    • v.36 no.2
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    • pp.417-428
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    • 1996
  • In order to know the toxic effect and carcinogenic activity in rats and mice fed with juice of Korean native Petasites japonicus Maxim of its pellet(4% or 8%) which were dried, milled and mixed with basal diet, the investigations were carried out by macroscopy and histopathology. Macroscopically, although remarkable changes were not observed in the liver of mice, there were slight to moderate swelling of rat livers in the whole groups at 12 to 14 weeks after feeding and milky spots in rats fed with its juice and 8% pelleted Petasites japonicus Maxim diet and a normal diet for 1 week alternatively for 14 weeks. Moreover, moderate to severe swelling and milk spots were recognized in livers of all rats fed with its juice and 8% pellet or 8% pelleted Petasites japonicus Maxim for 16 weeks. But, in cases of rats fed with its juice and 4% pellet or 4% pelleted Petasites japonicus Maxim, only swelling of livers was recognized moderately or severely. Histopathologically, major lesions were found in livers of both rats and mice. There were congestion, hemorrhage, fatty change, focal necrosis, megalocytosis and hyperplasia of endothelial cell in livers of mice and rats, the additional lesions such as proliferation of bile duct and nodular regeneration with diffuse regenerating cells were seen in livers of rats. In addition, preneoplastic lesions, the areas of milky spots macroscopically, were observed in livers of rats fed with Petasites japonicus Maxim for 14 to 16 weeks. In a few cases, haemangioendothelial sarcoma in livers was detected in rats fed with Petasites japonicus Maxim for 16 weeks. Petasites japonicus maxim growing naturally in Korea seem to exhibit toxic effect especially in liver and it contained a causative agent of primary liver tumors.

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Cytoprotective Effect of Petasites japonicus Extract on Cadmium-induced Cytotoxicity in HaCaT cell (Cadmium으로부터 손상을 유도한 HaCaT 세포에서 머위(Petasites japonicus) 추출물의 세포보호효과)

  • Kim, Bo-Ae
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.43 no.2
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    • pp.87-92
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    • 2017
  • In this study, we investigated the effect of Petasites japonicus extract on the cytotoxicity and cytoprotective effects against cadmium for cosmetics use. We measured the protein expression of apoptosis regulatory factor (Bcl-2 and procaspase-3) after treatment of Petasites japonicus extract in the cadmium-induced keratinocyte. As a result, high cell viabilities above 98% were observed in the all treated concentrations except at $200{\mu}g/mL$ of Petasites japonicus extract in keratinocytes with cadmium-induced damages. In keratinocytes with cadmium-induced damages, Bcl-2 and procaspase-3 protein expression increased in the experimental group treated with Petasites japonicus extract. Also HaCaT cells resulted in cleavage of PARP protein at 12 h post-cadmium exposure. Western blot analysis and relative density of the bands suggested that pretreatment of cells with Petasites japonicus extract inhibited cadmium-mediated cleavage of PARP. These results suggest that Petasites japonicus extract can be used as the cosmetic ingredients for cytoprotective effect.

Effect of Freeze-Drying and Hot Air-Convection Drying on the Antioxidative Activity of Butterbur (Petasites japonicus)

  • Cheong, Sun-Hee;Kim, Mi-Yeon;Son, Chan-Wok;Kim, Min-Hee;Lee, Yun-Jin;Kim, Mee-Ree
    • Food Quality and Culture
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    • v.2 no.2
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    • pp.61-66
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    • 2008
  • The principal objective of this study was to assess the anti oxidative activities of Petasites japonicus against oxidative stress in bovine brain tissue. Petasites japonicus is found with a relatively widespread distribution, and is cultivated as a culinary vegetable in Korea. Petasites japonicus samples were dried either by freeze-drying or by hot air-convection drying ($80^{\circ}C$), then evaluated for their anti oxidative activity by measuring 1-dipheny-1,2-picrylhydrazyl (DPPH) radical scavenging, and by measuring thiobarbituric acid-reactive substances (TBARS) in brain homogenates subjected to $Fe^{2+}$-mediated lipids with or without the addition of botanical extract. Hot air convection-drying resulted in a slight increase in the extraction yield as compared with freeze-drying. However, total phenol and flavonoid contents in freeze-dried Petasites japonicas were significantly higher than those of hot air convection-drying. Freeze-drying increased the free radical scavenging activity of Petasites japonicas, leaves, and stems by 52.6, 28.6, and 248.0%, as compared with hot air convection-drying. Additionally, the $IC_{50}$ values measured by TBARS in hot air convection-dried Petasites japonicas, leaves, and stems were increased by 36.0, 31.6, and 15.9%, as compared to those of freeze-drying. Although anti oxidative activity was reduced slightly by heat processing in Petasites japonicas, freeze-drying for each portion of Petasites japonicus was the most appropriate for use as a functional food and pharmaceutical material.

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Pathological Changes in Rats Fed petasites japonicus Maxim II. Immunohistochemical Localization of Cytochrome P4502E1 and GST-P in Liver

  • Jee, Young-Heun;Lee, Cha-Soo;Jeong, Kyu-Shik
    • Korean Journal of Veterinary Pathology
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    • v.1 no.1
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    • pp.33-39
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    • 1997
  • We investigated metabolism and carcinogenesis in livers of Sprague-Dawley rats fed juices and pelleted diets containing Korean native plants petasites japonicus Maxim by evaluating cell localization and expression of cytochrome P450s and GST-P. Anti-cytochrome P450s application in liver sections revealed three to four times increased expression of cytochrome P450E1 immunoreactivity in degenerative hepatocytes when compared to histologically normal hepatocytes. Anti-GST-P in showed positive pren plastic foci as well as in individual hepatocytes randomly scattered throughout all liver sections examined. Additionally GST-P was evident in proliferative endothelial cells and biliary epithelial cells in exposed rat livers. These results suggested that the increased level of cytochrome P4502E1 in affected hepatocytes was a direct consequence of Petasites japonicus toxicity. Further immunoreactivity to anti-GST-P in hepatocytes endothelial cells and biliary epithelial cells indicated a possible preneoplastic effects of Petasites japonicus in Sprague-Dawley rat.

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A Study on the Dyeing Properties of Petasites Japonicus Leaf Extract (머위 잎 추출액의 염색성 -매염제가 염색성에 미치는 영향-)

  • Kim Ae Soon;Chang Che Chul;Moon Un Joung
    • Textile Coloration and Finishing
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    • v.17 no.1
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    • pp.1-6
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    • 2005
  • This study were carried out to investigate the effects of mordants and mordanting methods under the various dyeing conditions such as temperature, time, pH, repetition of dyeing in the silk fabrics with Petasites japonicus leaf. It was the most high K/S values of the silk fabrics dyed with Petasites japonicus leaf according to co mordanting and used cupric sulfate, ferric sulfate as mordanting agents. K/S values of the silk fabrics dyed with Petasites japonicus leaf were increased gradually with dyeing temperature, dyeing time, repetition of dyeing was higher and the optimum pH was pH 7. Silk fabrics were colored yellowish orange and surface color(munsell value) was changed from 8.3YR to 2.0Y by using mordanting agents and those of the silk showed high tone when mordanting with cupric sulfate but decolored and darked when mordanting with ferric sulfate. Washing fastness of silk fabrics were good in 4 ~ 5 grade, so washing fastness of the silk fabrics was significantly improved when washed with the neutral detergent.

Effects of Petasites japonicus and Momordica charantia L. Extracts on MC3T3-E1 Osteoblastic Cells (머위(Petasites japonicus)와 여주(Momordica charantia L.) 추출물의 MC3T3-E1 조골세포 증식 및 분화에 미치는 효과)

  • Ji, Suk-Hee;Ahn, Do-Hwan;Jun, Mi-Ra
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.2
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    • pp.203-209
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    • 2010
  • In this study, the effects of Petasites japonicus and Momordica charantia L. extracts on MC3T3-ET1 osteoblastic cells were investigated. Since the activity of osteoblastic cell is one of the important factors for bone formation, the cellular proliferation of osteoblast was evaluated by MTT and alkaline phosphatase (ALP) activity. Compared to control, the cell proliferation was elevated to 114% and 112% by the treatment of Petasites japonicus and Momordica charantia L. extracts, respectively at the concentration of $10\;{\mu}g/mL$. The cell differentiation was also measured by alkaline phosphatase (ALP) activity at 3, 7, 14, and 27 days treatments with one of the extracts, respectively. As results, the ALP activity was significantly increased at 3 days, compared to control (p<0.05). To evaluate the effect of Petasites japonicus and Momordica charantia L. extracts on bone nodule formation, MC3T3-E1 cells were cultured in $\alpha$-MEM for 3, 14, and 21 days and then stained by alizarin red. To determine the expression patterns of bone-related proteins during the MC3T3-E1 osteoblast-like cell differentiation, osteoblast cells were cultured in $\alpha$-MEM for 24 hr. RNA was extracted and RT-PCR analysis was performed to examine the expression of OPG, RANKL and osteocalcin. Petasites japonicus extract exhibited the significant increment of osteocalcin compared with the positive control, which suggests that Petasites japonicus may have beneficial effects on bone health through the proliferation of osteoblast cells.

Dyeing Properties and Ultraviolet-cut Ability of Dyed Fabrics with Petasites japonicus Extract (머위 추출액에 의한 직물의 염색성과 자외선 차단성)

  • Choi, In-Ryu;Joen, Mi-Sun
    • The Research Journal of the Costume Culture
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    • v.19 no.1
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    • pp.96-103
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    • 2011
  • It is well known that the Petasites japonicus has been used for a long time medicine for the treatment of allergic diseases such as lacquer poisoning. However, the exact components and dyeing properties of its effects is still not known. Therefore, the purpose of this study was to investigate the dyeing property and ultraviolet-cut ability of silk and nylon fabrics that was dyed variously with the Petasites japonicus. The Petasites japonicus extract was done by boiling with distilled water at $100^{\circ}C$ for 1 hour. As mordanting agent, we used Aluminum potassium sulfate ($AlK(SO_4)_2{\cdot}12H_2O$), Copper(II) sulfate pentahydrate ($CuSO_5{\cdot}5H_2O$), Iron(II)Chloride ($FeCl_2{\cdot}4H_2O$). The best K/S value of dyeing temperature and time, all the fabrics were $100^{\circ}C$, 90min. Silk fabric was dyed yellow(0.8Y 7.6/2.2) and nylon fabric was dyed reddish yellow(10.1 YR 7.4/3.0). Silk fabric and nylon fabric was changed greenish yellow on mordanting with $CuSO_5{\cdot}5H_2O$ and $FeCl_2{\cdot}4H_2O$ respectively. And the colorfastness of washing and dry-cleaning was improved by using mordanting agent(4~5 grade). Ultraviolet-cut ability(UV-B) was showed more 90% in dyed nylon fabrics.

Optimization of a Process for Extraction of Petasin from Petasites japonicus Leaves by Response Surface Methodology (반응표면분석법에 의한 머위 잎의 petasin 추출공정 최적화)

  • Lee, Dong Wan;Lee, Se Yeul;Chung, Hun Sik;Choi, Young Whan;Im, Dong Soon;Lee, Young Guen
    • Journal of Life Science
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    • v.23 no.11
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    • pp.1360-1364
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    • 2013
  • Petasin extracted from Petasites japonicus leaves has been well known to be effective in the treatment of allergic asthma. This study was carried out to optimize the extraction process of petasin from P. japonicus leaves by response surface methodology (RSM). The dried powder of P. japonicus leaves was extracted at ethanol concentrations ranging from 40% to 80%, extraction rpm ranging from 125 rpm to 225 rpm, and extraction time ranging from 1 to 3 hours. The effects of the extraction conditions on the dry yield and petasin content of the extracts were investigated using a second-order Box-Behnken design. The petasin content was significantly affected by ethanol concentration, extraction rpm, and extraction time, tending to increase more with increasing ethanol concentration. The optimum condition for petasin extraction from Petasites japonicus leaves was 79.92% in ethanol concentration, 178.10 rpm in extraction rpm, and 2.06 hours in extraction time, respectively.

A New Furofuran Lignan with Antioxidant and Antiseizure Activities from the Leaves of Petasites japonicus

  • Min Byung-Sun;Cui Hui Song;Lee Hyeong-Kyu;Sok Dai-Eun;Kim Mee Ree
    • Archives of Pharmacal Research
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    • v.28 no.9
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    • pp.1023-1026
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    • 2005
  • A new furofuran lignan (1) was isolated from the n-butanol fraction of the methanolic extract of the leaves of Petasites japonicus (Sieb. et Zucc.) Maxim. (Compositae). The structure of compound 1 was determined to be $2{\alpha}-(4'-hydroxy-3'-methoxyphenyl)-6{\alpha}-(4"-hydroxy-3"-methox­yphenyl)-8{\alpha}-hydroxy-3, 7-dioxabicyclo[3.3.0]octane\;4'-O-({\beta}-D-glucopyranoside)$ by spectroscopic methods including 2D-NMR. In further studies, it was found that the compound 1 expressed an antioxidant activity in DPPH radical scavenging assay, and moreover, ameliorated the seizure in kainic acid-treated mice.