• Title, Summary, Keyword: Phellinus baumii

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Comparison on the Morphology, General Composition, Elemental Composition and Mineral Contents of Phellinus linteus, Phellinus baumii and Phellinus gilvus (Phellinus linteus, Phellinus baumii 및 Phellinus gilvus의 형태, 일반성분, 원소분석 및 무기성분 함량의 비교)

  • Bae, Jae-sung;Jang, Kwang-ho;Rhee, Man-hee;Jeong, Kyu-shik;Jo, Woo-sik;Choi, Sung-guk;Kim, Young-hoan;Park, Seung-chun
    • Korean Journal of Veterinary Research
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    • v.43 no.3
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    • pp.423-428
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    • 2003
  • The purpose of this study is to compare the morphology, general composition, elemental composition and mineral contents of Phellinus linteus, Phellinus baumii and Phellinus gilvus. In the scanning electron microscopy, P. gilvus and P. linteus had a similar shape. In the elemental composition, all of the three Phellinus spp. showed similar percent for the analyzed components. In the general composition, the water content of P. linteus. P. baumii and P. gilvus were 13%, 15% and 10%, respectively. The dietary fiber of three Phellius spp. showed more than 30%. The range of vitamin C and protein content was 1.5-2.1 g% and 3.63-3.73 g%. respectively. In mineral analysis, P. baumii of the three Phellinus spp. has the highest calcium concentration (1,135 ppm). From the above results, P. linteus. P. baumii and P. gilvus did not show any differences in the general composition and elemental composition.

Cultural Characteristics of Phellinus baumii Grown in Bottle (장수상황버섯(Phellinus baumii)의 병재배 특성)

  • Rew, Young-Hyun;Cho, Woo-Sik;Lee, Jin-Man;Kim, Jong-Guk
    • The Korean Journal of Mycology
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    • v.32 no.2
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    • pp.101-104
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    • 2004
  • For artificial cultivation of Phellinus baumii, we have conducted a study on cultural characteristics and condition of fruitbody formation in sawdust cultivation. Mycelial density and incubation rate were higher in oak sawdust+rice bran (4 : 1) medium than oak sawdust 100% medium. However, primordia and fruitbody formation were higher in oak sawdust 100% medium than rice bran added medium. Bigger bottle capacity (1,100 ml) resulted in a little higher yield and more biological efficiency than 850 ml bottle.

Molecular Detection of Phellinus linteus and P. baumii by PCR Specific Primer

  • Nam, Byung-Hyouk;Kim, Gi-Young;Park, Hyung-Sik;Lee, Sang-Joon;Lee, Jae-Dong
    • Mycobiology
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    • v.30 no.4
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    • pp.197-201
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    • 2002
  • Specific primer sets based on ribosomal DNA(rDNA) internal transcribed specer(ITS) sequences were designed for rapid detection of Phellinus linteus and P. baumii. Polymerase chain reaction(PCR) with these primers produced unique bands for each Phellinus species. The annealing temperature range is from $40^{\circ}C\;to\;55^{\circ}C$. The length of PCR products(P. linteus and P. baumii) using designed combinative primer sets of PL1F, PL2R, PB1F, PB2R, ITS5F and ITS4R, were from 520 by to 730 bp. Fifteen strains of Phellinus species including P. linteus, P. baumii, P. weirianus, P. johnsonianus, P. rhabarberinus, P. pini, P. gilvus, P. igniarius, P. nigricans and P. laevigatus were examined in this study. Five strains, including two isolated strains of P. linteus(MPNU 7001 and MPNU 7002), and two isolated strains of P. baumii(MPNU 7004 and MPNU 7005) were shown to have about 520 bp (PL1F-PL2R), 700 bp (TTS5F-PL2R) and 600 bp (PB1F-ITS4R) -sized PCR single bands respectively. This molecular genetic technique provided a useful method for rapid detection and identification of P. linteus and P. baumii.

Composition of Crude Polysaccharides in Some Phezllinus Species (수 종 상황버섯의 조다당 분획물 조성)

  • Park, Mi-Jung;Ahn, Mi-Jeong;Kim, Jin-Woong;Ze, Keum-Ryon;Lee, Heum-Sook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.1
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    • pp.131-135
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    • 2007
  • Various Phellinus species (Hymenochaetaceae) including P. linteus, P. baumii, P. pini, and P. igniarius of domestic and foreign products, were examined by gas chromatography to validate their composition of crude polysaccharides. The crude polysaccharide fraction, which is known to be one of the physiologically active fractions, were prepared by a cold ethanol precipitation method and the yield of the crude polysaccharide fractions of P. linteus, P. baumii, P. pini, and P. igniarius were 15.0%, 5.1%, 6.3% and 5.7%, respectively. The total monosaccharide amount of the crude polysaccharide fraction of P. linteus was 148.713 mg/g (dry weight of mushroom) while those of P. baumii, P. pini and P. igniarius were 29.022 mg/g, 29.222 mg/g and 1.546 mg/g, respectively. Although the major monosaccharide of all Phellinus mushrooms was glucose, there were remarkable differences in the absolute quantity of each monosaccharide. P. linteus included plenty of monosaccharides except mannose and ribose in comparison with P. baumii and P. pini. The absolute quantities of monosaccharides of P. baumii and P. pini were nearly similar except arabinose. The total monosaccharides of P. igniarius were minimal, but the portions of galactose and mannose in P. igniarius were greater than in the other species.

Genetic characterization of Phellinus baumii PMO-P4 by analyzing restriction fragment length polymorphisms of nuclear ribosomal DNA internal transcribed spacers (ITS) (Ribosomal DNA의 ITS부위에 대한 RFLP 분석에 의한 Phellinus baumii PMO-P4의 유전학적 특성)

  • Chang, Yun-Hee;Kim, Tae-Rack;Kim, Hyun-Su;Yeo, Ik-Hyun;Lee, Sang-Youn;Ha, Hyo-Cheol
    • Journal of Mushroom
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    • v.4 no.2
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    • pp.43-47
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    • 2006
  • PMO-P4, being cultivated as "Sanghwang" in Korea, was proved to be P. baumii based on ITS (internal transcribed spacer) sequencing and RFLP (Restriction Fragment Length Polymorphism) patterns along with some Phellinus species including P. linteus. The similaraty of ITS sequencing between PMO-P4 and other Phellinus species was given the range of 48.6%~72.2%, showing the highest homology from P. linteus and the lowest from P. gilvus.

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Exploring the quality standard of Phellinus spp through β-glucan content and sensory evaluation (β-glucan 함량과 관능검사를 통한 상황버섯 품질기준 모색)

  • Shin, Yong-Wook
    • The Korea Journal of Herbology
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    • v.32 no.4
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    • pp.47-52
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    • 2017
  • Objective : Phellinus spp. mushroom is an object of interest because it has excellent anticancer effect. Owing to the similalarities in the morphology, Phellinus linteus and Phellinus baumii are often used as same Sang Hwang Mushroom in the Korean market.. The quality control for mushrooms is needed because there are many differences in the efficacy according to cultivation method and cultivation area. Therefore, a reliable authentication method of these herbal medicine is necessary to compare and measure the amount of beta-glucan which is known to have a hypoglycemic effect, from the mushrooms collected in various regions Methods : 7 samples of medicinal mushrooms supplying phellinus spp. were collected in Korea, China and Cambodia. We investigated the hardness, colors, extract ratio, ${\alpha}-amylase$ and ${\alpha}-glucosidase$ inhibitory activities, glucose transporter 4 (GLUT-4) expressions of water extracts from Phellinus spp and also MTT assay were examined for cell toxicity. Results : The results revealed that Phellinus spp.water Ext.inhibited ${\alpha}-glucosidase$ activity. glucose transporter 4 (GLUT-4), the key insulin signaling pathway transcription factor, was remarkably increased by the Phellinus baumii water extract Conclusions : These results suggest that The more yellowish the mushroom is, the lower the hardness, the more the content of ${\beta}-glucan$ is proportional. Because the more ${\beta}-glucan$, the greater the effect of hypoglycemia. compared to the hypoglycemic effect, Phellinus Baumii grown at hanging on selves for 7 month in the green house is the best.

Taxonomical Classification and Species-specific Detection of Genus Some Phellinus using Phylotype (Phylotype에 의한 수종의 Phellinus속의 분류체계 확립 및 종간구별을 위한 신속동정법 개발)

  • Kim, Cheng-Yun;Lee, Jae-Yun;Kim, Gi-Young;Lee, Ki-Won;Park, Jae-Min;Kim, Mun-Ok;Lee, Tae-Ho;Lee, Jae-Dong
    • The Korean Journal of Mycology
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    • v.31 no.3
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    • pp.121-128
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    • 2003
  • This study was carried out to identify the phylogenetic relationship of Phellinus species and to know its distribution by comparing the DNA sequences of internal transcribed spacer regions(ITS1 and IST2) and 5.8S ribosomal DNA (rDNA) repeat unit. The Phellinus species had their specific sequences in IST1 and 2 regions depending on suedes. The comparison of the ITS sequences of standard strains indicated that the sequences of ITS1 were more variable than those of ITS2. Nine strains of the commercial products of Phellinus species used in this study were identified as P. lintues, P. baumii, P. igniarius, and P. pini. Most of commercial species were P. pini and P. baumii, and P. gilvus was not found. Also, P. linteus was only found in form of mycelial culture rather than fruiting body. Moreover, the species-specific primers were designed based on ITS sequence data. Each species-specific primers were bound in P. lintues(ITSF-PL2R), P. baumii(PB1F-ITS4R), P. igniarius(IF1-IR3), P. pini(PF1-PR3), and P. gilvus(GF2-GR4), respectively. These primer sets would be useful fer the detection of specific-species among unidentified Phellinus species rapidly.

Antioxidative-activity of Phellinus baumii Pilát in Caenorhabditis elegans (상황버섯의 예쁜꼬마선충 내의 항산화 효과)

  • Kim, Jun Hyeong;Kwon, Kang Mu;Yang, Jae Heon;Ki, Byeolhui;Kim, Dae Keun
    • Korean Journal of Pharmacognosy
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    • v.50 no.4
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    • pp.299-304
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    • 2019
  • In order to research the anti-oxidative activity of methanol extract of Phellinus baumii Pilat (Hymenochaetaceae), Caenorhabditis elegans model system was used. Ethyl acetate soluble fraction of the methanol extract showed the most potent DPPH radical scavenging activity. The ethyl acetate fraction was measured on its activities of superoxide dismutase (SOD), catalase, and oxidative stress tolerance with reactive oxygen species (ROS) level in C. elegans. Furthermore, to see if regulation of stress-response gene is responsible for the increased stress tolerance of C. elegans which treated by the ethyl acetate fraction, we checked SOD-3 expression using a transgenic strain. Consequently, the ethyl acetate fraction of P. baumii increased SOD and the catalase activities in a dose-dependent manner in C. elegans, reduced ROS accumulation dose-dependently. Besides, the ethyl acetate fraction-treated CF1553 worms showed higher SOD-3::GFP intensity than the control worms.

Inhibitory effects of Sanghwang mushrooms, Phellinus linteus strains and Phellinus baumii, on platelet aggregation (상황버섯, Phellinus linteus 계통과 Phellinus baumii의 항 혈소판 응집 효과)

  • Kang, Hee-Wan;Rhee, Man-Hee;Yeo, Woon-Hyung
    • Journal of Mushroom
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    • v.15 no.3
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    • pp.124-128
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    • 2017
  • The fruiting bodies of Sanghwang mushrooms, Phellinus linteus HN1009K (PLH) and Phellinus linteus (Korea Sanghwang, PLK), and Phellinus baumii (Jangsu Sangwhang, PB), were extracted with 70% methanol. The methanolic extracts of different concentrations ($5-200{\mu}g/ml$) showed inhibitory effects of 20-95% on plated aggregation induced by collagen (2.5 ug/ml), ADP (10 uM), and thrombin (0.1 U/ml). The PLH, PLK, and PB extracts (200 ug/ml) reduced ATP release from ADP-activated platelets by 50-60%. These results suggest that the methanolic extracts from Sanghwang mushrooms inhibit platelet aggregation.

Component(mineral) difference of Phellinus linteus on cultured to timber (기주식물에 따른 목질진흙버섯의 일반성분(무기물) 함량변화 I)

  • Lee, Soo-Won;Han, Ki-Won;Jang, Won-Cheoul;Lee, Dae-Jin;Jung, Dong-Sik;Lee, Byung-Eui
    • Journal of Mushroom
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    • v.3 no.3
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    • pp.100-104
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    • 2005
  • We investigated mineral difference from mushroom Phellinus linteus and Phellinus baumii on mulberry, oak and elm. ICP test, The major mineral components were K, Ca, and Fe, while Mg, Na, P, Cr, Mn, Cu and Zn were determined as micro mineral components. In case of P.linetus and P.baumii the component of K was shown as 699.0mg/100g and 630mg/100g, in oak, 340.7mg/100g and 314.6mg/100g in elm, and 311.6mg/100g and 311.6mg/100g in mulberry respectively. In Ca, mulberry fruiting body were shown about 2 times highest and expecially in case of Na was oak fruiting body were shown as 10 times highest as against the others sample groups. In micro mineral components, Zn component was shown very diffeence about sample groups. We concluded that the highest mineral component of K was shown in mushroom cultivated on oak of both P.linetus and P.baumii. As a results, mineral component has showed very difference on cultivated to timber. According to the results of mineral component we concluded that Phellinus linteus and Phellinus baumii has mineral component different depending on timber.

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