• Title, Summary, Keyword: Pholiota adiposa

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Extraction yield and nutraceuticals of mushroom Pholiota species (비늘버섯류의 추출수율 및 영양 약리학적 특성)

  • Cho, Soo-Muk;Park, Hong-Ju;Seo, Gun-Shik;Lee, Jong-Soo
    • Journal of Mushroom
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    • v.4 no.2
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    • pp.57-61
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    • 2006
  • This study was performed to obtain basal data for development of new functional food by using mushroom Pholiota spp.. Among several kinds of extracts, water extracts of Pholiota adiposa ASI 24027 showed the highest solid yield of 58.7%. Crude protein of Pholiota adiposa ASI 24015 and ${\beta}$-glucan contents of Pholiota adiposa ASI 24005 were 25.0% and 0.66%, respectively. K and Mg were also contained plentifully in the fruiting body of Pholiota adiposa ASI 24004 (3,649 mg and 138 mg/100g dried fruiting body, respectively). Amino acid and total fatty acid contents were similar between almost all of Pholiota spp..

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Pholiota adiposa and its Related Species Collected from the Wild Forestry (야생에서 채집된 검은비늘버섯(Pholiota adiposa)균에 관한 연구)

  • Lee, Sang-Sun;Kim, Mi-Hye;Chang, Hu-Bong;Shin, Chun-Sik;Lee, Min-Woong
    • The Korean Journal of Mycology
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    • v.26 no.4
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    • pp.574-582
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    • 1998
  • Five basidiocarps of Pholiota species have been collected from the areas of BubJu Temple for last two years, and identified to those of P. adiposa or Pholiota species. The taxonomy of these basidiocarps with the morphological aspects was compared with the analysis obtained from the polymorphisms of PCR-RAPD bands made after reacted with the random primers. The polymorphic variations were observed within the species of the basidiocarps, but not between genomic DNA's of the mycelia obtained and the basidiocarps. Several different bands made from the primers (28 and 36) in PCR-RAPD reactions were identified within the genus of Pholiota and speculated to be specific for the individual basidiocarp of P. adiposa collected. The primers employed here were considered to be very useful for distinguishing the individual isolates or basidiocarps collected from the fields. Also, the basidiospores were obtained from the sporeprints of the above basidiocarps as a simple agar and confirmed with observations of clamp connection under microscopes. The matings of them indicated the 'tetrapolar' type, being different from the 'bipolar' type reported by Japanese basidiocarps of P. adiposa or P. nameko. Based on our work, the edible fungi collected were speculated to be a new breeding resource for those of Pholiota commercialized in Japan.

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Effect of a Pholiota adiposa Extract on Fat Mass in Hyperlipidemic Mice

  • Cho, Soo-Muk;Lee, Young-Min;Lee, Dae-Hyoung;Chun, Hye-Kyung;Lee, Jong-Soo
    • Mycobiology
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    • v.34 no.4
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    • pp.236-239
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    • 2006
  • The purpose of this study was to investigate the effect of a Pholiota adiposa extract on fat mass in hyperlipidemic mice fed on a high-fat diet. The water extracts from P. adiposa (ASI 24018) were not affected in the total triglyceride contents and epididymal fat mass in mice fed on a high-fat diet, but the retroperitoneal fat mass decreased significantly. This result suggests that the P. adiposa extract may be a potential candidate for use as a functional food that can act as a prophylactic against hyperlipidemia. However, the P. adiposa extract showed no effect in the total triglyceride contents and epididymal fat mass.

The Fruit-body Formation and Properties of Pholiota sp. (비늘버섯속균(Pholiota sp.)의 특징과 자실체 형성)

  • Sung, Jae-Mo;Lee, Jae-Keun;Park, Dong-Soo
    • The Korean Journal of Mycology
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    • v.26 no.2
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    • pp.194-199
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    • 1998
  • Pholiota sp. were collected from the forest of Kangwon province (Mt. Samak, Mt. Odae and Kangwon National University Forest Experimentation) from the early of August to the end of October 1997. Among these fungi, Poliota adiposa was selected for culture experiment because it is suitable for edible mushroom. The Optimal temperature for the mycelial growth of Pholiota adiposa was in the range of $28^{\circ}C$ while that of fruit body formation was $15{\pm}1^{\circ}C$. Brown sugar as carbon source and soybean flour as nitrogen source were good for mycelial growth in commercial liquid culture. The fruit bodies of Pholiota adiposa were artifitially produced on plastic bottle including poplar sawdust and rice bran (4:1).

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Screening of Potent Anti-dementia Acetylcholinesterase Inhibitor-containing Edible Mushroom Pholiota adiposa and the Optimal Extraction Conditions for the Acetylcholinesterase Inhibitor

  • Kim, Do-Yeon;Bae, Sang-Min;Han, Sang-Min;Lee, Jong-Soo
    • The Korean Journal of Mycology
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    • v.44 no.4
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    • pp.314-317
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    • 2016
  • To develop a new anti-dementia acetylcholinesterase (AChE) inhibitor from edible mushrooms, AChE inhibitory activities were determined on water and ethanol extracts of various edible mushrooms from oriental medicine markets and agriculture markets. As a result, the 70% ethanol extract from Pholiota adiposa fruiting body had the highest AChE inhibitory activity of 30.6, and its water extract also had an AChE inhibitory activity of 23.8%. Therefore, we finally selected P. adiposa as a potent anti-dementia AChE inhibitor-containing mushroom. The AChE inhibitor of P. adiposa was maximally extracted when its fruiting body was treated with water for 3hr at $70^{\circ}C$ and 70% ethanol for 12 hr at $70^{\circ}C$, respectively.

Production and Characterization of Antihypertensive Angiotensin I-Converting Enzyme Inhibitor from Pholiota adiposa

  • Koo Kyo-Chul;Lee Dae-Hyoung;Kim Jae-Ho;Yu Hyung-Eun;Park Jeong-Sik;Lee Jong-Soo
    • Journal of Microbiology and Biotechnology
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    • v.16 no.5
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    • pp.757-763
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    • 2006
  • Angiotensin I-converting enzyme (ACE) inhibitors have generally been very useful to remedy or prevent hypertension. This study describes the extraction and characterization of an ACE inhibitor from the fruiting body of Pholiota adiposa ASI 24012, which can be used as an antihypertensive drug. The maximal ACE inhibitory activity $(IC_{50};0.25mg)$ was obtained when the fruiting body of Pholiota adiposa ASI 24012 was extracted with distilled water at $30^{\circ}C$ for 12 h. After the purification of ACE inhibitor with ultrafiltration, Sephadex G-25 column chromatography, and reverse-phase HPLC, an active fraction with an $IC_{50}$ of 0.044 mg was obtained. The purified ACE inhibitory peptide was a novel pentapeptide, showing very little similarity to other ACE inhibitory peptide sequences. The molecular mass of the purified ACE inhibitor was estimated to be 414 daltons with a sequence of Gly-Glu-Gly-Gly-Pro, and showed a clear antihypertensive effect on spontaneously hypertensive rats (SHR) at a dosage of 1 mg/kg.

Quality characteristics of muffins added with Pholiota adiposa powder (검은비늘버섯 분말을 첨가한 머핀의 품질특성)

  • Noh, Jae-Gwan;Yoon, Hyang-Sik;Oh, Eun Young;Kim, Ji Won;Kim, Sang Hee;Kim, Yee Gi;Han, Nam Soo;Eom, Hyun-Ju
    • Korean Journal of Food Preservation
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    • v.21 no.6
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    • pp.815-823
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    • 2014
  • This study was carried out to investigate the quality characteristics of muffins with added Pholiota adiposa powder. Muffins were prepared with different amounts of Pholiota adiposa powder (in 0, 2.5, 5, 10, and 15% ratios to the flour quantity). For analyzing the quality characteristics during the manufacturing of muffins, the pH, moisture content, dough density, height, specific volume, baking loss, texture, color, antioxidant activity, total polyphenol content, and sensory test results were determined. As the Pholiota adiposa powder content increased, the moisture content of the muffins decreased from 26.72 to 22.56%. The hardness of the muffins was increased with increasing Pholiota adiposa powder content, with the muffins with 15% Pholiota adiposa powder the hardest among all the samples. The inner color of the muffins, particularly the L and b values, decreased with increasing powder content compared to those of the control, but the a value increased. As the Pholiota adiposa powder content increased, the antioxidant activity and total polyphenol content of the muffins also increased. In the sensory evaluation, the muffins with 5% Pholiota adiposa powder showed higher values than the control and the other samples in all aspects, showing that 5% Pholiota adiposa powder could be incorporated into muffins to meet the taste and functional needs of the consumers.

Cytotoxic Chemical Constituents from the Mushroom of Pholiota adiposa

  • Chung, Ill-Min;Kong, Won-Sik;Lee, Oh-Kyu;Park, Jeong-Sik;Ahmad, Ateeque
    • Food Science and Biotechnology
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    • v.14 no.2
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    • pp.255-258
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    • 2005
  • The compounds, 1-Linoleic-2-olein (1), stigmasterol (2), 1,4-glucopyranosyl-1',4'-glucopyranosyl-1",4"-glucopyranoside (3), 2',3'-diphosphoryl-1'-propanoxy-${\beta}$-D-glucopyranoside (4), 1-Linoleic-3-olein (5), l-(N,N,N-trimethyl ethyl amino phosphoryl)-2,3-dilinolein ion (6) and glyceryl phosphate (7) were isolated and identified from the Mushroom of Pholiota adiposa for the first time by column chromatography, TLC, UV, IR, $^1H$ and $^{13}C$ NMR, EI-MS, and FAB-MS. The compounds 1 and 2 were found to have weak cytotoxicity against P388 murine leukemia cells. However, the compounds 6 and 7 did not show any cytotoxicity.

Quality Characteristics and Antioxidant Activities of Cookies with Pholiota adiposa Powder (검은비늘버섯 분말을 첨가한 쿠키의 품질특성 및 항산화활성)

  • Kim, Ji Won;Kim, Sang Hee;Yoon, Hyang-Sik;Song, Dal Nym;Kim, Min Ja;Chang, Who-Bong;Song, In Gyu;Eom, Hyun-Ju
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.12
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    • pp.1966-1971
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    • 2013
  • Pholiota adiposa is an edible as well as medicinal mushroom cultured in China and Japan with anti-tumor, anti-microbial, anti-hypertensive, and anti-hyperlipidemic activities. This study was carried out to investigate the quality characteristics of cookies added with Pholiota adiposa powder. Chemical composition of Pholiota adiposa powder was as follows: 3.8% moisture, 6.9% ash, 1% crude lipids, 3% protein, 53.3% carbohydrates, and 31.9% crude dietary fiber. To analyze quality characteristics during the manufacture of cookies, pH, texture, color, antioxidant activity, total polyphenol content, and sensory evaluation were determined. As the content of Pholiota adiposa powder increased, the pH of cookies decreased from 6.7 to 6.02. Hardness of cookies decreased with increasing Pholiota adiposa powder content, and cookies added with 5% powder were softer than the control. Regarding surface color, L-value decreased with increasing powder content compared to that of the control, whereas in surface color among various powder levels was not significantly different. As the content of Pholiota adiposa powder increased, both antioxidant activity and total polyphenol content increased. In the sensory evaluation, cookies added with 3% Pholiota adiposa powder showed remarkably higher values compared to control and other amples in all aspects.

Screening of Bioactive Compounds from Mushroom Pholiota sp. (비늘버섯으로부터 생리기능성 물질의 탐색)

  • Yu, Hyung-Eun;Cho, Soo-Muk;Seo, Geon-Sik;Lee, Byeong-Seok;Lee, Dae-Hyoung;Lee, Jong-Soo
    • The Korean Journal of Mycology
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    • v.34 no.1
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    • pp.15-21
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    • 2006
  • Extracts from 63 kinds of Pholiota sp. fruiting bodies were prepared using water and methanol, and then their physiological functionalities were investigated. The methanol extracts from Pholiota adiposa PAD030 showed high fibrinolytic activity and those of P. adiposa ASI PAD-022 showed potential inhibitory activity of 76.8% against ${\beta}-hydroxy-{\beta}-methylglutaryl(HMG)-CoA$ reductase. The highest antioxidant and tyrosinase inhibitory activities were found in the water extracts of Pholiota sp. PSP-015 (72.7%) and methanol extracts of P. nameko PNA-024 (69.5%), respectively. However, superoxide dismutase(SOD)-like activity and elastase inhibitory activity were low in almost of the extracts. The HMG-CoA reductase inhibitor from the fruiting body of P. adiposa PAD-022 which showed the highest functionality was extracted maximally when powder of the fruiting body was shaked at $30^{\circ}C$ for 12 h by methanol and its HMG-CoA reductase inhibitory activity was 80.2%.