• Title, Summary, Keyword: Pig

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Genomic analysis reveals selection signatures of the Wannan Black pig during domestication and breeding

  • Zhang, Wei;Yang, Min;Wang, Yuanlang;Wu, Xudong;Zhang, Xiaodong;Ding, Yueyun;Yin, Zongjun
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.5
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    • pp.712-721
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    • 2020
  • Objective: The Wannan Black pig is a typical Chinese indigenous, disease-resistant pig breed with high fertility, and a crude-feed tolerance that has been bred by artificial selection in the south of Anhui province for a long time. However, genome variation, genetic relationships with other pig breeds, and domestication, remain poorly understood. Here, we focus on elucidating the genetic characteristics of the Wannan Black pig and identifying selection signatures during domestication and breeding. Methods: We identified the whole-genome variation in the Wannan Black pig and performed population admixture analyses to determine genetic relationships with other domesticated pig breeds and wild boars. Then, we identified the selection signatures between the Wannan Black pig and Asian wild boars in 100-kb windows sliding in 10 kb steps by using two approaches: the fixation index (FST) and π ratios. Results: Resequencing the Wannan Black pig genome yielded 501.52 G of raw data. After calling single-nucleotide variants (SNVs) and insertions/deletions (InDels), we identified 21,316,754 SNVs and 5,067,206 InDels (2,898,582 inserts and 2,168,624 deletions). Additionally, we found genes associated with growth, immunity, and digestive functions. Conclusion: Our findings help in explaining the unique genetic and phenotypic characteristics of Wannan Black pigs, which in turn can be informative for future breeding programs of Wannan Black pigs.

Development of prevotella intermedia ATCC 49046 Strain-Specific PCR Primer Based on a Pig6 DNA Probe (Pig6 DNA probe를 기반으로 하는 Prevotella intermedia ATCC 49046 균주-특이 PCR primer 개발)

  • Jeong Seung-U;Yoo So-Young;Kang Sook-Jin;Kim Mi-Kwang;Jang Hyun-Seon;Lee Kwang-Yong;Kim Byung-Ok;Kook Joong-Ki
    • Korean Journal of Microbiology
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    • v.42 no.2
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    • pp.89-94
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    • 2006
  • The purpose of this study is to develop the strain-specific PCR primers for the identification of prevotella inter-media ATCC 49046 which is frequently used in the pathogenesis studies of periodontitis. The Hind III-digested genomic DNA of P. intermedia ATCC 49046 were cloned by random cloning method. The specificity of cloned DNA fragments were determined by Southern blot analysis. The nucleotide sequence of cloned DNA probes was determined by chain termination method. The PCR primers were designed based on the nucleotide sequence of cloned DNA fragment. The data showed that Pig6 DNA probe were hybridized with the genomic DNA from P. intermedia strains (ATCC $25611^T$ and 49046) isolated from the Westerns, not the strains isolated from Koreans. The Pig6 DNA probe were consisted of 813 bp. Pig6-F3 and Pig6-R3 primers, designed base on the nucleotide Sequences Of Pig6 DNA Probe, were 3150 specific to the only both P. intermedia ATCC $25611^T$ and P. intermedia ATCC 49046. In the other hand, Pig6-60F and Pig6-770R primers were specific to the only P. intermedia ATCC 49046. The two PCR primer sets could detect as little as 4 pg of chromosomal DNA of P. intermedia. These results indicate that Pig6-60F and Pig6-770R primers have proven useful for the identification of P. intermedia ATCC 49046, especially with regard to the maintenance of the strain.

Identification of copy number variations using high density whole-genome single nucleotide polymorphism markers in Chinese Dongxiang spotted pigs

  • Wang, Chengbin;Chen, Hao;Wang, Xiaopeng;Wu, Zhongping;Liu, Weiwei;Guo, Yuanmei;Ren, Jun;Ding, Nengshui
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.12
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    • pp.1809-1815
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    • 2019
  • Objective: Copy number variations (CNVs) are a major source of genetic diversity complementary to single nucleotide polymorphism (SNP) in animals. The aim of the study was to perform a comprehensive genomic analysis of CNVs based on high density whole-genome SNP markers in Chinese Dongxiang spotted pigs. Methods: We used customized Affymetrix Axiom Pig1.4M array plates containing 1.4 million SNPs and the PennCNV algorithm to identify porcine CNVs on autosomes in Chinese Dongxiang spotted pigs. Then, the next generation sequence data was used to confirm the detected CNVs. Next, functional analysis was performed for gene contents in copy number variation regions (CNVRs). In addition, we compared the identified CNVRs with those reported ones and quantitative trait loci (QTL) in the pig QTL database. Results: We identified 871 putative CNVs belonging to 2,221 CNVRs on 17 autosomes. We further discarded CNVRs that were detected only in one individual, leaving us 166 CNVRs in total. The 166 CNVRs ranged from 2.89 kb to 617.53 kb with a mean value of 93.65 kb and a genome coverage of 15.55 Mb, corresponding to 0.58% of the pig genome. A total of 119 (71.69%) of the identified CNVRs were confirmed by next generation sequence data. Moreover, functional annotation showed that these CNVRs are involved in a variety of molecular functions. More than half (56.63%) of the CNVRs (n = 94) have been reported in previous studies, while 72 CNVRs are reported for the first time. In addition, 162 (97.59%) CNVRs were found to overlap with 2,765 previously reported QTLs affecting 378 phenotypic traits. Conclusion: The findings improve the catalog of pig CNVs and provide insights and novel molecular markers for further genetic analyses of Chinese indigenous pigs.

Development of a Geometry PIG for the Inspection of Natural Gas Pipeline and It´s application

  • Kim, Dong-Kyu;Cho, Sung-Ho;Park, Seoung-Soo;Park, Dae-Jin;Koo, Sung-Ja;Yoo, Hui-Ryong;Park, Yong-Woo;Kho, Young-Tai
    • 제어로봇시스템학회:학술대회논문집
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    • pp.150.4-150
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    • 2001
  • The geometry PIG provides pipeline operators with continuous measurement of pipe centerline coordinates, bend radius, displacement, and bending strain in a single pass through the pipeline. This study introduces the developed geometry PIG(Pipeline Inspection Gauge) which is used for geometry surveys. This tool is equipped with the several sensor systems. The Inertial Navigation System (INS) comprises angle rate gyros and linear accelerometers. The system measures the precise path of the PIG during its traverse of the pipeline. This system is also used to produce a detailed map of the lire, measure curvature. Odometers measure the PIG´s distance moved along the line and instantaneous speed during the PIG run. Caliper sensors measure pipeline ...

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Progress in Transgenic Cloned Pig for Xenotransplantation

  • Park, Kwang-Wook
    • Proceedings of the Korean Society of Embryo Transfer Conference
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    • pp.9-19
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    • 2003
  • Pig organ is thought to be the most suitable nonhuman organ for xenotransplanstation. However, one of the major constraints to using pig organs for xenotransplantation is human natural antibody-mediated hyperacute rejection (HAR). Elimination of a(1,3) galactosyltransferase (GGTA1) from the pig is expected to be a solution to the problem of hyperacute rejection. ry1any efforts have made characterization of GGTA1 in structure and function. improvement in the technique of DNA transfection of somatic cells and advancement of the pig NT, a specific modification has been made to one copy of the GGTA1 gene by Missouri group in 2002. To date because homozygousity of the genetic modification has been achieved in this gene, the role of gala(1,3) gal specific natural antibody in HAR and the efficacy of xenotransplantation in a nonhuman primate model will be addressed. If other genes are found to be involved in rejection of pig donors by primates, the technology will be available to modify those genes so that rejection can be overcome.

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Umbilical Hernia and Repair in a Transgenic Male Cloned Pig

  • Kim, Geon A;Jin, Jun-Xue;Taweechaipaisankul, Anukul;Lee, Sanghoon;Kim, Min Jung;Lee, Byeong Chun
    • Journal of Veterinary Clinics
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    • v.35 no.5
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    • pp.226-228
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    • 2018
  • We generated a transgenic male cloned pig which was derived from fibroblast of white Yucatan miniature pig. After 2 weeks of birth, umbilical hernia which was not easily reduced was identified. Considering the usefulness of cloned pig, surgical treatment for umbilical hernia correction was performed and a cloned pig has been maintained healthy. This is the first report and can be useful for the treatments of umbilical hernia of cloned piglets.

Dynamic Modeling of PIG Flow through Curved Section in Natural Gas Pipelines (천연가스배관내 곡선 영역을 지나는 피그흐름의 동적모델링)

  • Nguyen, Tan Tien;Yoo, Hui-Ryong;Rho, Yong-Woo;Kim, Sang-Bong
    • Proceedings of the KSME Conference
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    • pp.247-252
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    • 2001
  • In this paper, dynamic modeling and its analysis for the PIG flow through $90^{\circ}$ curved pipe with compressible and unsteady flow are studied. The PIG dynamics model is derived by using Lagrange equation under assumption that it passes through 3 different sections in the curved pipeline such that it moves into, inside and out of the curved section. The downstream and up stream flow dynamics including the curved sections are solved using MOC. The effectiveness of the derived mathematical models is estimated by simulation results for a low pressure natural gas pipeline including downward and upward curved sections. The simulation results show that the proposed model and solution can be used for estimating the PIG dynamics when we pig the pipeline including curved section.

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Progress in Transgenic Cloned Pig for Xenotransplantation

  • Park, Kwang-Wook
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • pp.9-19
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    • 2003
  • Pig organ is thought to be the most suitable nonhuman organ for xenotransplanstation. However, one of the major constraints to using pig organs for xenotransplantation is human natural antibody-mediated hyperacute rejection (HAR). Elimination of a(1,3) galactosyltransferase (GGTA1) from the pig is expected to be a solution to the problem of hyperacute rejection. Many efforts have made characterization of GGTA1 in structure and function, improvement in the technique of DNA transfection of somatic cells and advancement of the pig NT, a specific modification has been made to one copy of the GGTAl gene by Missouri group in 2002 To date because homozygousity of the genetic modification has been achieved in this gene, the role of gala(1,3) gal specific natural antibody in HAR and the efficacy of xenotransplantation in a nonhuman primate model will be addressed. Of other genes are found to be involved in rejection of pig donors by primates, the technology will be available to modify those genes so that rejection can be overcome.

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Effect of Insameumja Extract on the Contraction of Isolated Guinea Pig Trachea Smooth Muscle (인삼음자(人蔘飮子)가 GUINEA PIG의 기관지(氣管支) 평골근(平滑筋)에 미치는 영향(影響))

  • Sim, Young-Seop;Han, Jong-Hyeon;Han, Sang-Whan
    • The Journal of Internal Korean Medicine
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    • v.17 no.1
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    • pp.193-203
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    • 1996
  • These studies were carried out to investigate the effect of Insameumja water extract on the inhibitory contractile action of acetylcholine in guinea pig. The results of these studies were as follows; 1. Contractile force of acetylcholine from trachea smooth muscle in guinea pig was significantly inhibited by Insameumja. 2. Dose-response of acetylcholine pretreated Insameumja in guinea pig was not significantly changed. 3. Inhibitory contractile action of acetylcholine pretreated propranolol in guinea pig was decreased. 4. Inhibitory contractile action of acetylcholine pretreated indomethacin was significantly changed by Insameumja. 5. Inhibitory contractile action of acetylcholine pretreated methylene blue was not significantly changed by Insameumja.

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Effect of Mahwangchangchultang Extract on the Contraction of Isolated Guinea Pig Trachea Smooth Muscle (마황창출탕(麻黃蒼朮湯)이 GUINEA PIG의 기관지(氣管支) 평골근(平滑筋)에 미치는 영향(影響))

  • Park, No-Su;Han, Sang-Hwan;Han, Jong-Hyeon
    • The Journal of Internal Korean Medicine
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    • v.16 no.2
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    • pp.92-100
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    • 1995
  • These studies were carried out to investigate the effect of Mahwangchangchultang water extract on the inhibitory contractile action of acetylcholine in guinea pig. The results of these studies were as follows; 1. Contractile force of acetylcholine from trachea smooth muscle in guinea pig was significantly inhibited by Mahwangchangchultang 2. Dose-response of acetylcholine pretreated Mahwangchangchultang in guinea pig was not significantly changed. 3. Inhibitory contractile action of acetylcholine pretreated propranolol in guinea pig was decreased. 4. Inhibitory contractile action of acetylcholine pretreated indomethacin was not significantly changed by Mahwangchangchultang. 5. Inhibitory contractile action of acetylcholine pretreated methylene blue was not significantly changed by Mahwangchangchultang.

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