• Title, Summary, Keyword: Poly(l-lactide)

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Preparation and Release Behavior of Albumin-Loaded PLGA Scaffold by Ice Particle Leaching Method (얼음입자추출법을 이용한 알부민 함유 PLGA 담체의 제조 및 방출 거동)

  • Hong Keum Duck;Seo Kwang Su;Kim Soon Hee;Kim Sun Kyung;Khang Gilson;Shin Hyung Sik;Kim Moon Suk;Lee Hai Bang
    • Polymer(Korea)
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    • v.29 no.3
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    • pp.282-287
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    • 2005
  • A novel ice particle leaching method for fabrication of porous and biodegradable PLGA scaffold has been proposed for the application to tissue engineering. After uniform mixing of poly(L-lactide-co-glycolide) (PLGA) and bovine serum albumin-fluorescein isothiocyanate (FITC-BSA), the FITC-BSA loaded scaffold was fabricated by adding various ratio of ice particle. The release profiles of FITC-BSA were examined using pH 7.4 PBS for 28 days at $37^{circ}$. The release amount was determined by fluorescence intensity by using the fluorescence spectrophotometer and the morphological change of the scaffolds was observed by scanning electron microscope. The release initial burst of BSA containing scaffolds was lower than that of simple dipping scaffolds resulting in constant release aspect. Although the BSA concentration increased. the initial burst was not increased. As a result of this study, it can be suggested that ice particle leaching method for the tissue engineered scaffold miff be very useful and it is possible to impregnate with water soluble factors like cytokine. We suggest that ice particle leaching method may be useful to tissue engineered organ regeneration.

The Control of Drug Release and Vascular Endothelialization after Hyaluronic Acid-Coated Paclitaxel Multi-Layer Coating Stent Implantation in Porcine Coronary Restenosis Model

  • Bae, In-Ho;Jeong, Myung Ho;Kim, Ju Han;Park, Yong Hwan;Lim, Kyung Seob;Park, Dae Sung;Shim, Jae Won;Kim, Jung Ha;Ahn, Youngkeun;Hong, Young Joon;Sim, Doo Sun
    • Korean Circulation Journal
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    • v.47 no.1
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    • pp.123-131
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    • 2017
  • Background and Objectives: Hyaluronic acid (HA) is highly biocompatible with cells and the extracellular matrix. In contrast to degradation products of a synthetic polymer, degradation products of HA do not acidify the local environment. The aim of this study was to fabricate an HA-coated paclitaxel (PTX)-eluting stent via simple ionic interactions and to evaluate its effects in vitro and in vivo. Materials and Methods: HA and catechol were conjugated by means of an activation agent, and then the stent was immersed in this solution (resulting in a HA-coated stent). After that, PTX was immobilized on the HA-coated stent (resulting in a hyaluronic acid-coated paclitaxel-eluting stent [H-PTX stent]). Study groups were divided into 4 groups: bare metal stent (BMS), HA, H-PTX, and poly (L-lactide)-coated paclitaxel-eluting stent (P-PTX). Stents were randomly implanted in a porcine coronary artery. After 4 weeks, vessels surrounding the stents were isolated and subjected to various analyses. Results: Smoothness of the surface was maintained after expansion of the stent. In contrast to a previous study on a PTX-eluting stent, in this study, the PTX was effectively released up to 14 days (a half amount of PTX in 4 days). The proliferation of smooth muscle cells was successfully inhibited (by $80.5{\pm}12.11%$ at 7 days of culture as compared to the control) by PTX released from the stent. Animal experiments showed that the H-PTX stent does not induce an obvious inflammatory response. Nevertheless, restenosis was clearly decreased in the H-PTX stent group ($9.8{\pm}3.25%$) compared to the bare-metal stent group ($29.7{\pm}8.11%$). Conclusion: A stent was stably coated with PTX via simple ionic interactions with HA. Restenosis was decreased in the H-PTX group. These results suggest that HA, a natural polymer, is suitable for fabrication of drug-eluting stents (without inflammation) as an alternative to a synthetic polymer.