• Title, Summary, Keyword: Polygonum cuspidatum

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Antithrombotic Effect and Antiplatelet Activity of Polygonum cuspidatum Extract (호장근 추출물의 항혈전 효능 및 혈소판 응집 억제작용)

  • Yang, Won-Kyung;Sung, Yoon-Young;Kim, Ho-Kyoung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.2
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    • pp.168-173
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    • 2012
  • We performed this study to develop antithrombotic agents from oriental medicine herb extracts. Polygonum cuspidatum has been traditionally used as an edible medical resources for the treatment of cancer, pyodermatitis, hepatitis, cystitis, and inflammation. However, the effects of Polygonum cuspidatum on thrombosis and platelet activation are not precisely understood. The antithrombotic and antiplatelet activities of Polygonum cuspidatum were investigated by assessing the effect of a 70% ethanol extract of Polygonum cuspidatum on blood coagulation, fibrinolysis, and platelet aggregation. Polygonum cuspidatum showed effective fibrinolytic activity at 10 mg/mL. Polygonum cuspidatum also inhibited adenosine diphosphate induced platelet aggregation. Furthermore, evaluation of anticoagulant activity showed that an extract of Polygonum cuspidatum prolonged coagulation time via activated partial thromboplastin time (APTT). Our results show that Polygonum cuspidatum can be a potential candidate for antiplatelet activity as well as a fibrinolytic agent.

Inhibition of Nitric Oxide Production by Anthraquinones from Polygonum cuspidatum (호장으로부터 분리한 안트라퀴논류의 Nitric Oxide 저해활성)

  • Joo, Si-Mong;Yang, Ki-Sook
    • YAKHAK HOEJI
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    • v.54 no.5
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    • pp.387-391
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    • 2010
  • Polygonum cuspidatum which is widely distributed in Korea has been used as treatments of dermatitis, gonorrhea and inflammation in traditional medicine. We examined anti-inflammatory activities by the inhibition of NO production in RAW264.7 murine macrophages cells. Phytochemical examination of Polygonum cuspidatum led to the isolation and characterization of emodin (1), emodin 8-O-${\beta}$-D-glucopyranoside (2), emodin 1-O-${\beta}$-D-glucopyranoside (3). Antioxidative activities of these compounds were determined by measuring the radical scavenging effects on DPPH radicals. Compounds 1 and 3 showed potent activities compared with L-NMMA. These results suggested that the antraquinone compounds isolated from Polygonum cuspidatum might be used as antiinflammatory agents.

Antilipoperoxidant Activity of Antraquinone and Stilbene from Polygonum cuspidatum (호장으로부터 분리한 안트라퀴논 및 스틸벤 화합물의 지질과산화 저해활성)

  • Joo, Si-Mong;Lee, Min-Won;Yang, Ki-Sook
    • YAKHAK HOEJI
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    • v.51 no.2
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    • pp.140-144
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    • 2007
  • Polygonum cuspidatum has been used as treatments of dermatitis, gonorrhea, inflammation, and hyperlipidaemia in traditional medicine. We examined liver protective effect on CCl$_4$ inducing hepatotoxicity and anti-oxidative activity by TBA method. Phytochemical examination of Polygonum cuspidatum led to the isolation and characterization of emodin 8-O-${\beta}$-D-glucopyranoside (compound 1), and trans-resveratrol 3-O-${\beta}$-D-glucopyranoside (compound 2). Compounds 1 and 2 enhanced the inhibition of anti-lipid peroxidative effects in liver homogenate. In chemical parameters obtained from serum analysis, compounds 1 and 2 also revealed significant decrease in hepatotoxicity. These results suggested that the antraquinone and stilbene which were isolated from Polygonum cuspidatum might be used as therapeutic agent of hepatitis.

The Effects of Polygonum Cuspidatum on the Skin Functions (호장근추출물이 피부질환 및 피부미용에 미치는 영향)

  • Jung, Hyun-A;Roh, Seok-Seon;Oh, Min-Suck
    • Journal of Korean Medicine Rehabilitation
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    • v.19 no.1
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    • pp.73-89
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    • 2009
  • Objectives : This study was carried out to investigate the effects of Polygonum cuspidatum extract on several skin functions including inflammation and wrinkle formation. Methods : To investigate in vitro anti-oxidant activity assay, ethanol extracts of medicinal plants tested by DPPH method. In the next experiment, to investigate anti-inflammatory test, the RAW 264.7 macrophage cells was cultured using DMEM including the 10% FBS. To study anti-allergic effect, we blended cultured Human Mast Cells(HMC-1), and then observe $TNF-{\alpha}$, IL-8 by ELISA Results : Polygonum Cuspidatum extract has the effects of anti-inflammation and anti-allergy, which may be due to its inhibitory potential on the macrophage activation. Furthermore, Polygonum Cuspidatum extract has the anti-wrinkle effects through the inhibitory potential on the collagnease, elastase and gelatinase activities. Conclusions : The above results suggest that Polygonum Cuspidatum extract could be applicable for improvement of several skin functions.

Natural dyeing of silk fabric with Polygonum cuspidatum (호장근을 이용한 견직물의 천연염색)

  • Kim, Sangyool
    • The Research Journal of the Costume Culture
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    • v.23 no.5
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    • pp.768-777
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    • 2015
  • In this study, the dyeing of silk fabric with Polygonum cuspidatum extracts was investigated. The contents of this study are as follows. First, the proper dyeing conditions were investigated by measuring the dye uptake (K/S value) that depended on the dyeing conditions when silk fabric was dyed with Polygonum cuspidatum extract. Second, the brightness (L), hue, and chroma differences that appear after mordanting with Al, Cu and Fe were investigated by measuring the CIELAB and Munsell values. And third, the colorfastness and antibacterial property were measured. When the silk fabric was dyed with Polygonum cuspidatum extract, the proper dyeing conditions were a colorant concentration of 90% v/v, a dyeing of time 100 minutes, a dyeing temperature of $70^{\circ}C$, and a dyeing of pH 3. In mordanting methods, the dyeabilities of post-mordanting were higher than those of premordanting. The hue value displayed yellow (Y) and yellow-red (YR) in cases of pre and post mordanting. The C value decreased by the mordanting of Polygonum cuspidatum extracts. Generally the colorfastness of mordanted fabrics was improved by mordanting. The dyed fabrics showed a 90.6% of Staphylococcus aureus reduction rate, and the dyed and mordanted fabrics showed 97.1% bacteria reduction rate. The dyed and mordanted fabrics showed above 90.5% Klebsiella pneumoniae reduction rate, and Cu mordant revealed the most effective bacterial reduction.

Polygonum cuspidatum Extract Induces Apoptosis in Human Uterine Cervical Carcinoma ME-180 Cells (호장근(虎杖根)이 자궁경부암세포(子宮經部癌細胞)의 성장억제(成長抑制) 및 세포고사(細胞枯死)에 미치는 영향)

  • Choi, Byun-Tak;Kim, Yeon-Hee;Lee, Dong-Nyung;Kim, Hyung-Jun
    • The Journal of Korean Obstetrics and Gynecology
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    • v.19 no.1
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    • pp.1-13
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    • 2006
  • Purpose : Polygonum cuspidatum extract is an oriental herb which has been used for uterine diseases. In this study, the effects of Polygonum cuspidatum extract were investigated on inducing growth inhibition and apoptosis of human uterine cervical carcinoma cells. Methods : Viability of Polygonum cuspidatum extract-induced ME-180 cells was measured by MTT assay. Apoptotic cells were visualized by EtBr/AcOr staining under fluorescent microscope. Nucleosomal DNA fragmentation was assayed by agarose gel electrophoresis. Cell cycle distribution and changes in mitochondrial membrane potential were observed by flow cytometry. Results : Polygonum cuspidatum extract induced ME-180 cell death in a dose- and time-dependent manner. In the cells treated with Pc, the population of cells at sub-G1 phase significantly increased, and the condensed nuclei, apoptotic bodies and nucleosome-sized DNA were detected. Moreover, reduction in mitochondrial membrane potential was detected. Conclusion : Polygonum cuspidatum extract inhibits the growth and proliferation of ME-180 cells by apoptotic induction and facilitates its activity initiated by depolarization of mitochondria.

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Pharmacognostical Studies on Polygonum Species (호장엽의 생약학적 연구)

  • Kim, Tae-Hee;Lee, Chong-Hee
    • Korean Journal of Pharmacognosy
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    • v.4 no.2
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    • pp.75-82
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    • 1973
  • In Korea, two species of Ho-chang-Ho-chang $(Polygonum\;cuspidatum \;S_{IEB}.\;et\;Z_{UCC}.)$ and Wang-Ho-chang $(Polygonum \;sachalinense\;F_R.\;S_{CHM}.)$-had been identified, but the components of the leaves of Polygonum species growing in Korea had never been reported. The authors examined the anatomical characteristics of Korean Polygonum species, identified the flavonoids contained in their leaves and obtained the following results: Korean Polygonum species should be divided into three species: $Polygonum\;cuspidatum\;S_{IEB}.\;et\;Z_{UCC}.,\;P. \;sachalinense\;F_R.\;S_{CHM}\;and\;P.\;elliptica\;M_{IGO}$. The last one was illustrated in the report. P. cuspidatum contained reynoutrin and quercitrin, but no isoquercitrin could be identified. P. sachalinense also contained both reynoutrin and quercitrin. P. elliptica, which had never been studied, included the same flavonoids.

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Changes of Plasma and Hepatic Lipids, Hydroxy-Methyl-Glutaryl CoA Reductase Activity and Acyl-CoA : Cholesterol Acyltransferase Activity by Supplementation of Hot Water Extracts from Rosa rugosa, Crataegus pinnatifida and Polygonum cuspidatum in High-Cholesterol Fed Rats

  • Lee, Hee-Ja;Park, Myung-Sook
    • Preventive Nutrition and Food Science
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    • v.3 no.4
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    • pp.344-350
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    • 1998
  • Lipid lowering properties from three plant water extracts, Rosa rugosa, Crataegus pinnatifida and Polygonum cuspidatum, were tested by supplementing a 1% high-cholesterol diet with them in rats. Plasma triglyceride levels in Rosa fugosa, Crataegus pinnatifida and Polygonum cuspidatum groups were significantly lower compared to that of the control. by 29% , 24% and 47% respectively. hepatic trigylceride levels in Rosa rugosa and Crataegus pinnatifida groups were significantly lower compared to the control by 11% and 15% respectively. Hepatic HMG-CoA reductase activity in Rosa rugosa group was significantly greater compared to the control by 406%. Hepatic ACAT activity was significantly lower in Polygonum cuspidatum group compared to the control by 28%. by multiple regression results, only plasma cholesterol was associated significantly (p<0.05) with liver HMG-CoA reductase activity. Plasma cholesterol explained 12% of thevariance of the liver HMG-CoA redctase activity. In conclusion, we have showen that hot water extracts from Rosa rugosa, Crataegus pinnatifida and Polygonum cuspidatum lowered plasma triglycerides in rats fed on a high-cholesterol diet. Data suggests that these extracts could potentially prevent or treat hypertriglyceridemia induced by a high fat diet and fatty liver.

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Antilipoperoxidant Activity of the Root Polygonum cuspidatum on Carbon Tetrachloride Induced Hepatotoxicity (호장근의 사염화탄소로 유도된 지질과산화 저해활성)

  • 김지연;양기숙
    • YAKHAK HOEJI
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    • v.43 no.5
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    • pp.572-576
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    • 1999
  • The root of Polygonum cuspidatum (Polygonaceae) has been used as treatments of hyperlipidemia, dermatitis, gonorrhea, favus athlete's foot, inflammation in traditional medicine. In order to examine anti-lipidperoxidation activity, hexane, EtOAc, BuOH and water fractions of its methanol extract were administered to carbon tetrachloride intoxicated rats. Ethylacetate fraction exhibited antilipidperoxidative effect on liver lipid homogenate and the radical scavenging effect on DPPH. Serum transaminase, AlP, triglyceride and total cholesterol contents significantly decreased by administrations of ethylacetate fraction.

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Inhibition of Whole Blood Platelet aggregation from Traditional medicines (한약재의 전혈혈소판응집억제)

  • Jeon, Won-Kyung;Kim, Jung-Hee;Lee, A-Yeong;Kim, Ho-Kyoung
    • Korean Journal of Oriental Medicine
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    • v.9 no.2
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    • pp.55-67
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    • 2003
  • To evaluate anti-aggregatory activity of traditional prescriptions and medicines for treatment of Ohyul symptom, 70% methanol extracts were examined using collagen stimulated in vitro platelet aggregation by impedance method in rat whole blood. The crude extracts from DoHaekSeungKiTang, BoYangHwanOhTang, Caesalpinia sappan, Rhus verniciflua, Rheum palmatum, Polygonum cuspidatum, Salvia miltiorrhiza were found to inhibit platelet aggregation. The effective crude extracts of traditional medicine were fractionated to dichloromethane, ethyl acetate, butanol and aqueous layer. Polygonum cuspidatum, Caesalpinia sappan aud Rhus verniciflua ethyl acetate fractions concentration-dependently $(250-50{\mu}m/ml)$ inhibited the aggregation of platelet in whole blood induced by collagen. These results suggested that ethyl acetate fractions of Polygonum cuspidatum, Caesalpinia sappan and Rhus verniciflua have potent anti-aggregatory activity.

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