• Title, Summary, Keyword: Pulsed electromagnetic field

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The Effects of Pulsed Electromagnetic Field on Functional Recovery and Expression of GAP-43 after Incomplete Spinal Cord Injury in Rats (맥동전자장이 불완전 척수손상 흰쥐의 기능회복과 GAP-43의 발현에 미치는 영향)

  • Bang, Hyun-Soo
    • Journal of the Korean Society of Physical Medicine
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    • v.7 no.3
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    • pp.349-356
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    • 2012
  • Purpose : This study was designed to investigate the effects of pulsed electromagnetic field on functional recovery and expression of GAP-43 after incomplete spinal cord injury (SCI) in rats. Methods : To confirm the damage of SCI and effects of pulsed electromagnetic field, 20 Sprague-Dawley male rats were used and divided randomly 2groups (SCI, PEMF). Incomplete SCI was induced by using modified NYU drop model. After operation, functional recovery test, immunohistochemistry and western blot analysis were measured at 1, 2, 3 weeks. Pulsed electromagnetic field were apply three weeks (one times a day, five days a week and twenty minutes a session). Results : In the this study, applications of pulsed electromagnetic field after incomplete SCI induced the significant improvement in functional recovery and expression of neurotrophic factor. The results were as follows; Foot print test, PEMF were significantly decreased than the SCI (p<.05). Expression of GAP-43, PEMF were significantly increased than the SCI at 2 and 3 weeks (p<.05). Conclusion : In conclusion, pulsed electromagnetic field were positive effect in functional recovery and expression of GAP-43 after incomplete SCI in rats.

The Effects of Pulsed Electromagnetic Fields on Blood components, Antioxidant enzymes and Reactive Oxygen in Hyperlipidemic Rats (맥동전자장이 고지혈증 흰쥐의 혈액 성분, 항산화 효소 및 활성 산소에 미치는 영향)

  • Bang, Hyun-Soo;Jeong, In-Ho;Lee, Sang-Deok
    • Journal of Digital Convergence
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    • v.12 no.7
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    • pp.349-356
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    • 2014
  • The present study was aimed to investigate the effects of the application of pulsed electromagnetic fields on the blood components associated hyperlipidemia, antioxidant enzymes and reactive oxygen. The subjects were divided into three groups: General Diet, High fat Diet, High fat diet and pulsed electromagnetic fields. Pulsed electromagnetic field was applied with pulsed electromagnetic energy therapy equipment. The Glucose, free fatty acids, triglycerides, cholesterol and insulin was used to measure hyperlipidemia-related blood components, and the GSH, GRD, XO and MDA was used to measure antioxidant enzymes and reactive oxygen. The applied pulsed electromagnetic field has improved the concentrations of blood components and increasing GSH and GRD, that were decreased due to a high-fat diet and by reducing the increased MDA and XO to a level of general diet group. Therefore, the applied pulsed electromagnetic field can be the effective treatment for changes in the blood composition of hyperlipidemic rats, enhancement of antioxidative activity and the inhibition of reactive oxygen.

A STUDY OF EFFECT OF PULSED ELECTROMAGNETIC FIELDS ON OSTEOGENESIS IN RABBIT CRANIAL BONE DEFECT (가토 두개부 골결손에서 맥동전자기장이 골형성에 미치는 영향에 관한 연구)

  • Hwang, Kyung-Gyun;Lee, Jong-Hwan;Kim, Myung-Jin;Shim, Kwang-Sup;Kim, Jong-Won
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.28 no.4
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    • pp.264-273
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    • 2002
  • Pulsed electromagnetic field (PEMF) was used first to induce osteogenesis in 1974. The appliance which was consisted of the Helmholtz coil configuration have used to osteogensis. The objective of this study was to determine whether PEMF, a frequency of 100 Hz and magnetic field strength of 38 gauss applied to the calvarial defect in rabbit, could affect the induction of osteogenesis and the healing of the graft bone. This field should not produce excitation of nerve or muscle and heating the tissue. To evaluate the effect of PEMF on osteogenesis, 16 rabbit under the same condition was divided into 8 experimental groups and 8 control groups. 10 mm calvarial bone defects were formed around sagittal suture. The defect of left side was left without graft while the defect of right side was grafted by bone harvested from left side. A pulsed electromagnetic field was applied for 8 hours per day. Each group was sacrificed after 1 week, 2 weeks, 4 weeks, 8 weeks. Microscopic specimens were obtained from the calvarial bone defects and surrounding tissue using Hematoxylin-Eosin staining method. The results were as follows. 1. In the group which pulsed electromagnetic field was applied, new bone formation filled up the defect was observed after 4 and 8 weeks effectively. 2. There are no difference in the healing period for the fusion between the bone and graft bone. According to the result, the PEMF with 38 Gauss, 100 Hz was very effective in the healing of bone defect and new bone formation. So The PEMF will be useful in clinical aspect for oseteogenesis.

The Effects of Pulsed Elctromagnetic Fields on Expression of Neurotrophic Factors after Spinal Cord Hemisection in Rats

  • Kang, Ji-Hyuk;Park, Sang-Young;Lee, Yun-Seob
    • Journal of Magnetics
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    • v.16 no.3
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    • pp.253-258
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    • 2011
  • The purpose of this study was to identify the effect of pulsed electromagnetic fields on the expression of neurotrophic factors after spinal cord injury. Sprague-Dawley male rats were given a spinal cord hemisection and randomly divided into 2 groups, the control and experimental groups. The experimental group was administered a fifteen minutes session of pulsed electromagnetic field once a day, five days a week. In order to observe the effect of these pulsed electromagnetic fields, this study observed the BDNF expression in the rat's lumbar spinal cord and the H&E staining in the gastrocnemius at 3, 7, 14, 21 days group after spinal cord hemisection. The results of this showed that the immunoreactivity of the BDNF in the rat's spinal cord gradually increased in each group. At 21 days, there is a significant difference between the control and experimental groups. The morphological shape of the gastrocnemius was gradually changed from 3days to 21days, and the gastrocnemius at 21 days was significantly degraded. However, the experimental group showed a slightly more organized gastrocnemius than the control group at 21days. The Results of this study suggest that pulsed electromagnetic field application decreases the degeneration of a rat's gastrocnemius morphology, and increases the immunoreactivity of the BDNF in the rat's spinal cord after spinal cord hemisection.

Pulsed Ultrasound and Pulsed Electromagnetic Field in the Treatment of Muscle Contusion in Rats

  • Cheon, Song-Hee;Lee, Sun-Min
    • Journal of Magnetics
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    • v.17 no.3
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    • pp.225-228
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    • 2012
  • Muscle contusion usually results from a direct blunt impact and is frequently associated with contact sports. Muscle contusion results from microscopic muscle fiber and capillary disruption causing a microhemorrhage dissecting torn fibers and remaining viable muscle fibers. Recent studies concluded that some physical methods, including pulsed ultrasound (PU) and pulsed electromagnetic field (PEMF) treatment, accelerate and facilitate wound healing, improve scar quality and have beneficial effects on muscle and tendon healing. However, there are few studies on the effects of the early use of physical methods, such as PU and PEMF, on the expression of neurotrophic factors. The objective of this study was to investigate the effects of the early application of PU and PEMF, measured through the expression of BDNF in the muscles (gastrocnemius) and spinal cords of rats after skeletal muscle contusion. In the spinal cords and muscles, there was a significant increase of BDNF expression in the PEMF and PU groups, a greater increase was found in the PEMF group than in the PU group. In conclusion, PEMF is a useful therapeutic method that improves muscle healing after muscle contusion.

The Effects of Static Magnetic Field and Pulsed Electromagnetic Field on Alkaline Phosphatase and DNA synthetic Activity of ME3T3-E1 Cells (정자기장과 맥동전자기장이 MC3T3-E1 세포의 ALP 및 DNA 활성도에 미치는 영향)

  • Son, Jeong-Hee;Bae, Seong-Min;Sung, Jae-Hyun
    • The korean journal of orthodontics
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    • v.27 no.4
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    • pp.623-632
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    • 1997
  • The purpose of this study was to evaluate the effects of magnetic field on cellular activity of MC3T3-E1 cells. The cellular activity was monitored by alkaline phosphatase and DNA synthetic activity in control, static magnetic field and pulsed electromagnetic field groups. A static magnetic field was applied to the cell by placing one, two, three, foue, and five samarium-cobalt magnets above and below each cell plate for 24hours per day. A pulsed electromagnetic field with a frequency of 100 herz was applied for 10 hours per day. After 10 days of magnetic field exposure, there were increase of alkaline phosphatase activity in static magnetic field groups consisted of one, two and three magnetic groups. Alkaline phosphatase activities were not significantly increased in four and five magnetic groups. Application of pulsed electromagnetic field did not result in significant increase in alkaline phosphatase activity compared to control. DNA synthetic activity in both static and pulsed electromagnetic field group were not significantly different from that in control group. The result of this study suggest that magnetic field could have effect on the metabolism of bone cells related to the cellular metabolic process.

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Acceleration of Cell Proliferation and Gene Expression in Human Chondrosarcoma Cells Stimulated by Strong Pulse Magnetic Field

  • Shin, Sung Chul;Chung, Eui Ryong;Hwang, Do Guwn
    • Journal of Magnetics
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    • v.18 no.1
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    • pp.14-20
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    • 2013
  • For the treatment of osteoarthritis, pulsed electromagnetic field stimulus has been suggested as a useful therapeutic method in rehabilitative medicine. Most studies have been performed under low-frequency and low-energy to find out biological properties for stimulating chondrocyte with pulsed magnetic field. In this study, the effect of strong pulse magnetic field on the human chondrosarcoma cells (SW-1353) has been investigated by means of cell counting, morphologies, and gene expression of cartilage extracellular matrix genes. The SW-1353 cells were exposed under the field intensities of 270, 100, 55, 36, and 26 mTesla during 6 hours a day in 5 consecutive days. The pulse magnetic field with an LRC oscillating signal has the pulse width of 0.126 msec and stimulation period of 1 sec. For the 270 and 100 mTesla stimulation, the cell proliferation significantly increased in 21-24% as compared with the non-stimulated cells. Gene expression of cartilage extracellular matrix genes (ACAN, COMP and COL2A1) was assayed by quantitative real time-PCR method. The ACAN gene expression showed a significant brightness, which means the increase on gene expression, compared with the non-stimulated cells. Our results suggest that the strong pulse magnetic field stimulation can be utilized to accelerate cell proliferation and gene expression on human chondrosarcoma cells.

An Experimental Study on the Effect of Half Sine-Wave Pulsed Electromagnetic Field in Orthodontic Tooth Movement (Half-sine Wave Pulsed Electromagnetic Fields가 교정적 치아 이동에 미치는 영향에 관한 실험적 연구)

  • Kim, Young-Joon
    • The korean journal of orthodontics
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    • v.29 no.2
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    • pp.157-163
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    • 1999
  • The purpose of this study was to investigate the effect of electromagnetic field in orthodontic tooth movement, and the optimal application period of electromagnetic field to initiate tooth movement. Eighty male Hartley guinea pigs were utilized in this study. Laterally directed orthodontic force was applied to the maxillary central incisors. Half sine-wave pulsed electromagnetic field(HSPEMP) was applied to experimental group during 10 days. According to the period of application of HSPEMP(4 hours, 8 hours, 12 hours a day), they were divided into the three sub-groups The obtained results were as follows; 1. In case of application of HSPEMF during 4 hours a day, there was no significant difference in the final amount of orthodontic tooth movement between the experimental and the control group. 2. In case of application of HSPEMF during 8 and 12 hours a day, there was a significant increase in the final amount of orthodontic tooth movement since the eighth day, the sixth day respectively. 3. In comparison with the case of application of HSPEMF during 8 hours a day, there was a significant increase in the final amount of orthodontic tooth movement at the seventh day and the eighth day in case of application of HSPEMF during 12 hours a day.

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A STUDY ON THE ORTHODONTIC TOOTH MOVEMENT UNDER HALF SINE-WAVE PULSED ELECTROMAGNETIC FIELDS (Half sine-wave pulsed electromagnetic fields 내에서 교정력에 의한 치아 이동에 대한 연구)

  • Kim, Young-Joon;Ryu, Young-Kyu
    • The korean journal of orthodontics
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    • v.24 no.4
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    • pp.897-916
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    • 1994
  • The biologic potential, which is different from the piezoelectric signals, relates tooth movement at least in part to changes in bone metaboliosm in bioelectric theory. The purpose of this experiment was to determine wheather the application of half sine-wave pulsed electromagnetic fields (HSPEMF) could increase both the rate and amount of orthodontic tooth movement. Forty-three male Hartley guinea pigs, weighting approximately 255g, were utilized in this study. The animals were 35 days old at the start of the study. Laterally directed orthodontic force was applied to the maxillary central incisors of 40 Hartley guinea pigs (20 experimental, 20 control). According to the amount of orthodontic force (6g, 12g), they were divided into two sub-groups (10 experimental I, 10 experimental II, 10 control I, 10 control II). During the experimental period, experimental animals were placed in plastic animal holders with their heads positioned in an area of uniform electromagnetic field. Control animals were placed in similar plastic holders that did not carry the electric apparatus. The results were as follows : 1. The application of a HSPEMF to the experimental groups significantly increase the final amount of orthodontic tooth movement observed over a 10-day experimental period. 2. The application of a HSPEMF to the experimental groups significantly increase the velocity of orthodontic tooth movement observed over a 10-day experimental period. 3. There was no significant difference in the final amount of orthodontic tooth movement at the fourth day to the eighth day, but there was significant difference in the final amount of orthodontic tooth movement at the nineth, tenth day during a 10-day experimental period. 4. After 10 days of HSPEMF exposure & orthodontic force, the experimental groups demonstrated more osteodasts in the pressure side than control groups.

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IME EFFECT OF PULSED ELECTROMAGNETIC FIELD ON THE CULTURED CALVARIAL CELLS OF RAT (맥동 전자기장이 백서의 배양 두개관세포에 미치는 영향)

  • Choi, Byung-Taek;Yang, Won-Sik
    • The korean journal of orthodontics
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    • v.20 no.3
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    • pp.499-517
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    • 1990
  • Electrical stimulation among several factors that influence bone remodeling has been studied by many investigators with great enthusiasm in orthodontic field. The action mechanisms of Pulsed Electromagnetic Field (PEMF) are different from those of the conventional electrode application method in that PEMF induces endogenous current in the living tissues. PEMF is known to have the healing effect in nonunion of bone and osteoporosis. It is widely used in orthopaedic scopes and the possibility of using the method in clinical orthodontics Is also conceivable. But the exact mechanisms by which the PEMF exerts its effects are not clearly understood. Therefore, the author wanted to see the effect of PEMF on five groups of rat calvarial cells obtained by sequential enzyme digestion method, and observed the changes in enzyme activation, collagen synthesis and $^3H-thymidine$ incorporation. The results were as follows: 1. Under the effect of PEMF, there were no changes in the alkaline phosphatase activity in five groups of cell populations. 2. Both the PEMF group and the PTH with PEMF group shelved no changes in acid phosphatase activities and there were no differences between two experimental groups. 3. Under the effect of PEMF, there was significant increase of collagen synthesis in the group V cell population. 4. Under the effect of PEMF, there were significant increases of $^3H-thymidine$ incorporation in the group IV and V cell populations.

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