• Title, Summary, Keyword: Pyrus pyrifolia

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Lupane Triterpenoids from Pyrus pyrifolia

  • Yoo, Ji-Hye;Yang, Ki-Sook
    • Natural Product Sciences
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    • v.18 no.1
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    • pp.13-15
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    • 2012
  • Three lupane type triterpenoids were isolated from the methanol extract of Pyrus pyrifolia fruit peel through repeated silica gel column chromatography. Based on the spectroscopic methods, their structures were determined to be lupeol (1), betulin (2), and betulinic acid (3).

Application of crude enzymes obtained from Pyrus pyrifolia cv. Shingo on milk proteins

  • Park, Min-Gil;Kim, Hyoung-Sub;Nam, In-Sik;Kim, Woan-Sub
    • Korean Journal of Agricultural Science
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    • v.45 no.4
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    • pp.789-797
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    • 2018
  • This study investigated the activity of crude enzymes obtained from Pyrus pyrifolia cv. Shingo on milk proteins. In the milk processing industry, there is an increasing interest in the addition of functional materials to dairy products or functional peptides isolated from milk proteins. First, Pyrus pyrifolia cv. Shingo was separated into core, flesh, and peel regions, and crude enzymes were obtained from the individual regions. The activity of the obtained crude enzymes was measured using casein and gelatin agar. The crude enzyme obtained from the flesh of Pyrus pyrifolia cv. Shingo decomposed gelatin, but the activity of the crude enzymes obtained from the peel and core regions was insignificant. On the other hand, the crude enzymes obtained from the flesh and core regions of Pyrus pyrifolia cv. Shingo had a remarkable enzymatic activity in casein agar. However, the activity of the crude enzyme obtained from the peel region was insignificant. In addition, the crude enzymes obtained from the individual regions were mixed with casein to induce reactions, and the degradation patterns were investigated through electrophoresis and high performance liquid chromatography (HPLC). According to the results, the crude enzymes from Pyrus pyrifolia cv. Shingo degraded milk proteins. Thus, the results of this study can be used in studies on functionality. Additionally, it is expected that the use of pear peels and cores in the milk processing industry would greatly contribute to the reduction of food waste.

Constituents of Pyrus pyrifolia with Inhibitory Activity on the NO Production and the Expression of iNOS and COX-2 in Macrophages and Microglia

  • Yoo, Ji-Hye;Yang, Ki-Sook
    • Natural Product Sciences
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    • v.18 no.3
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    • pp.183-189
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    • 2012
  • It is well known that inflammation is associated with neurodegenerative disorders, including Alzheimer' disease, Parkinson's disease and ischemia. Nitric oxide (NO), a pro-inflammatory mediator, is produced by inducible NO synthase (iNOS) in microglia as well as macrophages and appears to account for neurodegeneration. In this study, we aimed to isolate NO inhibitors from Pyrus pyrifolia by activity guided purification. As a result, we identified daucosterol and ${\beta}$-sitosterol, which have not been isolated from this plant before. This article also describes NO inhibitory activities of the methanol extract of Pyrus pyrifolia fruit and the isolated compounds from this, which are lupeol, betulin, betulinic acid, ${\beta}$-sitosterol and daucosterol, in LPS-activated RAW 264.7 and BV2 cell lines. Western blot analysis was performed to clarify the underlying mechanism of NO inhibition in the two cell lines.

Protective Effects of Pyrus pyrifolia NAKAI Leaf Extracts on UVB-induced Toxicity in Human Dermal Fibroblasts (자외선B 노출로 인해 손상된 피부세포에 대한 돌배나무잎 추출물의 보호효과)

  • Koh, Ara;Choi, Songie;Kim, Yong-ung;Park, Gunhyuk
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.42 no.1
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    • pp.87-94
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    • 2016
  • Skin damage is mainly caused by environmental factors such as ultraviolet light, heat, and smoking. It is known that reactive oxygen species production is commonly involved in the pathogenesis of skin damage induced by these factors, causing skin aging. Pyrus pyrifolia Nakai continues to be a popular and highly consumed fruit in many countries with known beneficial effects including antitumor, antioxidative, and anti-inflammatory effects. However, there is no evidence of a therapeutic effect of Pyrus pyrifolia extract (PPE) against skin aging via inhibition of mitochondria-mediated apoptosis. In this study, we investigated PPE protective effect against photoaging induced by UVB ($50mJ/cm^2$) in HS68 human dermal fibroblasts. Lactate dehydrogenase assay showed that PPE significantly protected HS68 cells against UVB-induced damage in a dose-dependent manner. Other assays using DCF-DA demonstrated that PPE protected HS68 cells by regulating reactive oxygen species production. PPE also regulated mitochondrial dysfunction and mitochondrial membrane potential induced by UVB, and inhibited UVB-induced caspase-3 activity. These results indicate that PPE protects human dermal fibroblasts from UVB-induced damage by regulating the oxidative defense system.

Construction of a Genetic Map using the SSR Markers Derived from "Wonwhang" of Pyrus pyrifolia (배 '원황'(Pyrus pyrifolia) 유전체 해독에 기반한 SSR 마커 개발 및 유전자 지도 작성)

  • Lee, Ji Yun;Seo, Mi-Suk;Won, So Youn;Lim, Kyoung Ah;Shin, Il Sheob;Choi, Dongsu;Kim, Jung Sun
    • Korean Journal of Breeding Science
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    • v.50 no.4
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    • pp.434-441
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    • 2018
  • High-density genetic linkage mapping is critical for undertaking marker-assisted selection and confirming quantitative trait loci, as well as helping to build pseudomolecules of genomes. We constructed a genetic map using 94 $F_1$ populations generated from the interspecific cross between Korean cultivar "Wonwhang" (Pyrus pyrifolia, NCBI BioSample SAMN05196235) and European cultivar "Bartlett" (Pyrus communis). We designed a total of 24,267 SSR markers based on the genome sequences of "Wonwhang" for this. To select the markers that are linked to the traits important in pear breeding programs, SSR-containing genomic sequences were subjected to nucleotide sequence homology searches, which resulted in 510 SSR markers with high similarity to genes encoding proteins with putative functions such as transcription factors, resistance proteins, flowering time, and regulatory genes. Of these, 70 markers showed polymorphisms in parents and segregating populations and were used to construct a genetic linkage map, together with the unpublished 579 SNPs obtained from genotyping by sequencing analysis. The genetic linkage map covered 3,784.2 cM and the average distance between adjacent markers was 5.8 cM. Seventy SSR markers were distributed across 17 chromosomes with more than one locus.

Isolation and Identification of Two Flavonoids from Pear (Pyrus pyrifolia Nakai cv. Chuhwangbae) Fruit Peel (추황배(Pyrus pyrifolia Nakai cv. Chuhwangbae) 과피로부터 2종의 Flavonoids의 단리·동정)

  • Lee, Sang Won;Lee, Yu Geon;Cho, Jeong-Yong;Kim, Young Chool;Lee, Sang-Hyun;Kim, Wol-Soo;Moon, Jae-Hak
    • Korean Journal of Food Science and Technology
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    • v.47 no.2
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    • pp.170-175
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    • 2015
  • The methanol extract of Asian pear (Pyrus pyrifolia N. cv. Chuhwangbae) fruit peel was purified using solvent fractionation, Sephadex LH-20 column chromatography, and octadecylsilane high performance liquid chromatography. Based on the electrospray ionization mass spectrometry and nuclear magnetic resonance data, the two isolated compounds were identified as quercetin 3-O-${\beta}$-D-glucopyranoside (1) and 3,5,6,7,8,3',4'-heptahydroxyflavan [(-)-dulcisflavan, 2]. Compounds 1 and 2 were isolated and identified for the first time from Asian pears and pears, respectively.

Study on the Antioxidant Effect and Total Phenolics Content in Rosaceae Plant Stem (장미과 식물 줄기의 항산화 효과와 총 페놀류 함량에 관한 연구)

  • Lee, Jun-Young;You, Ju-Han;Kim, Sang-Wook
    • Journal of Environmental Science International
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    • v.23 no.12
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    • pp.2129-2134
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    • 2014
  • The antioxidant activities and total phenolics of four Rosaceae species Pourthiaea villosa (Thunb.) Decne, Sorbus commixta Hedlund, Sorbaria sorbifolia var. stellipila Maxim and Pyrus pyrifolia (Burm.f.) Nakai were determined. Phenolic content (polyphenol and flavonoid), radical scavenging activities [2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylnezthiazoline-6-sulfoic acid) (ABTS)] and ferrous ion chelating effect were evaluated. Total polyphenol and flavonoid contents were highest in Pourthiaea villosa (Thunb.) Decne and lowest in Pyrus pyrifolia (Burm.f.) Nakai. Phenolic contents of Pourthiaea villosa (Thunb.) Decne was $331.45{\pm}7.78$ and $90.4{\pm}3.5mg{\cdot}g^{-1}$. DPPH and ABTS radical scavenging activities were found to be lowest in Sorbus commixta Hedlund whereas Sorbaria sorbifolia var. stellipila Maxim and Pourthiaea villosa (Thunb.) Decne showed relatively good DPPH and ABTS radical scavenging activities. Ferrous ion chelating effect was highest in Pyrus pyrifolia (Burm.f.) Nakai ($1.05{\pm}0.04mg{\cdot}ml^{-1}$) and lowest in Sorbus commixta Hundlund ($4.22{\pm}0.71mg{\cdot}ml^{-1}$).

Effects of Pear (Pyrus pyrifolia) Methanol Extracts on the Proliferation and the Cytokines Production of Mouse Splenocytes (배(梨)의 메탄올 추출물이 마우스의 비장세포 증식능과 Cytokine 생성능에 미치는 영향)

  • Hwang Yoo-Kyung;Pyo Myoung-Yun
    • YAKHAK HOEJI
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    • v.49 no.1
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    • pp.25-29
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    • 2005
  • This study was performed to investigate the potential of pear (Pyrus pyrifolia) as a immune-modulating functional food by assay of splenocytes proliferation and induction of cytokines (IFN-${\gamma}$, IL-4) in vitro. When mouse splenocytes were exposed to various concentration (0.16, 0.31, 0.63, 1.25, 2.50 mg/ml) of pear methanol extracts (P-M) without mitogens, splenocytes proliferation (SP) was significantly increased. Also, SP to mitogens, concanavalin A (Con A) and lipopolysaccharide (LPS) were significantly increased by P-M when compared with controls. When splenocytes were cultured with P-M in the presence of Con A, cytokine (IFN-${\gamma}$, IL-4) levels in culture supernatant were significantly enhanced in a dose-dependent manner except 2.5 mg/ml when compared with control group. Therefore, our study suggest that the pear has the potential of being an immune-modulating functional food.

Ecological characteristics of areas naturally inhabited by Dolbae trees(Pyrus pyrifolia) on Mt. Kaya

  • Ahn, Young-Hee;Chung, Kyu-Hwan
    • Journal of Environmental Science International
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    • v.11 no.11
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    • pp.1149-1156
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    • 2002
  • The ecological characteristics of Dolbae trees, a valuable genetic resource for native pyrus fruit trees, were investigated at four naturally inhabited areas on Mount Kaya, Gyeongsangnam-Do. Most Dolbae trees were found on the southwest side of an old hiking path to a mountain valley at altitudes of 610∼670 m. The surrounding flora consisted of 46 families, 69 genera, 75 species, and 10 varieties. The natural habitat of the Dolbae trees was basically a deciduous broad-leaved forest with a predominance of Quercus serrata and ligneous plants(xylophytes) from the Fagaceae and Betulaceae families. The habitat was also found to be in a second transition resulting from forest disturbance and exhibited a degree of diversity of 2.901-5.065, based on Shannon-Weiner's index. Examination of the Dolbae trees found in Mount Kaya revealed diameter breast heights(DBHs) ranging from 10 to 60 cm, including six old and large Dolbae trees with a DBH of 50∼60 cm, which will be particularly valuable as a genetic resource. Accordingly, the current results suggest that the study areas are a secondary forest in a typical mid-temperate zone resulting from forest damage about 10 years earlier.