• Title/Summary/Keyword: REBA

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Analysis of REBA, RULA, OWAS of Wearing and Taking off Skinscuba Equipment for Prevention of Musculoskeletal Injuries (스킨스쿠버 장비 착용 및 벗는 자세의 근골격계 상해 예방을 위한 REBA, RULA, OWAS 분석)

  • Son, Sung-Min;Kim, Yong-Jae
    • Journal of Fisheries and Marine Sciences Education
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    • v.23 no.1
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    • pp.67-74
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    • 2011
  • The study was constructed an analysis of postures of a expert diver's wearing and taking off with skinscuba equipment using ergonomics tools(REBA, RULA, OWAS) for prevention of Musculoskeletal injuries. This survey is consisted of 4 postures in terms of wearing and taking off : Crouching(alone), Standing(alone), Helping of assistance, Using of stanchion. Their rate of injuries is getting higher due to instability postures, exposed dangers and excessive physical actions. So this study provides basic and educational information for prevention of that. The result is as follows : The result of crouching postures of wearing alone was REBA 9(Ac Level 3), RULA 7(Ac Level 4), OWAS AC 2, and crouching postures of taking off alone was REBA 12(Ac Level 4), RULA 7(Ac Level 4), OWAS AC 4. The result of standing postures of wearing alone was REBA 8(Ac Level 3), RULA 7(Ac Level 4), OWAS AC 3, and standing postures of taking off alone was REBA 8(Ac Level 3), RULA 7(Ac Level 4), OWAS AC 3. The result of helping of assistance postures of wearing was REBA 4(Ac Level 2), RULA 3(Ac Level 2), OWAS AC 2, and standing postures of taking off alone was REBA 4(Ac Level 2), RULA 4(Ac Level 2), OWAS AC 1. The result of using of stanchion postures of wearing was REBA 3(Ac Level 1), RULA 3(Ac Level 2), OWAS AC 1, and standing postures of taking off alone was REBA 3(Ac Level 1), RULA 3(Ac Level 2), OWAS AC 1. Especially both postures of wearing and taking off alone showed high results in the analysis of postures using ergonomics tools(REBA, RULA, OWAS).

Comparison of Posture Classification Schemes of OWAS, RULA and REBA (작업 자세 평가 기법 OWAS, RULA, REBA 비교)

  • Kee, Do-Hyung;Park, Kee-Hyun
    • Journal of the Korean Society of Safety
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    • v.20 no.2 s.70
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    • pp.127-132
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    • 2005
  • The purpose of this study is to compare representative posture classification schemes of OWAS, RULA and REBA in terms of correctness for postural load. The comparison was based on the evaluation results by the three methods for 224 working postures sampled from steel, electronics, automotive, and chemical industries. The results showed that OWAS and REBA generally underestimated postural stress than RULA irrespective of industry type, work performed and whether or not leg posture is balanced. While about $71\%\;and\;73\%$ of the 224 posture were evaluated with the action category/level 1 or 2 by OWAS and REBA respectively, about $60\%$ of the postures were classified into the action level of 3 or 4 by RULA. The coincidence rate of postural stress category between OWAS and RULA was just $33.5\%$, while the rate between RULA and REBA was $46.0\%$. It is concluded from the findings of this study and the previous research that compared to OWAS and REBA, RULA more precisely evaluates postural stress.

Analysis of Accuracy and Reliability for OWAS, RULA, and REBA to Assess Risk Factors of Work-related Musculoskeletal Disorders (근골격계질환 유해요인 정밀조사를 위한 OWAS, RULA, REBA의 평가 정확도 및 신뢰도 분석)

  • Cheon, Woohyun;Jung, Kihyo
    • Journal of the Korea Safety Management & Science
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    • v.22 no.2
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    • pp.31-38
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    • 2020
  • The study evaluated the accuracy and intra-rater reliability for OWAS (Ovako Working posture Analysing System), RULA (Rapid Upper Limb Assessment), REBA (Rapid Entire Body Assessment) to improve their evaluation accuracy and reliability. Participants (n = 163) with undergraduate degree were recruited in this study and trained for 6 hours about the ergonomic assessment methods. Ergonomic assessments were conducted using OWAS, RULA, and REBA for a representative work with dynamic posture found in manufacturing industries. The study compared action categories (overall level) and detailed evaluation scores for individual body part. Action categories of the participants significantly differed from the golden reference defined by ergonomic experts. The participants underrated or omitted scores for truck (37.4% of the participants) and legs (52.8%) in OWAS. Similarly, the participants underrated or omitted additional scores for all body parts except the hand and wrist in RULA (53.5%) and REBA (54.8%). On the other hand, the participants overrated scores for the hand and wrist in RULA (55.2%) and REBA (39.9%). The results found in this study can help of selecting focus points and parts during assessment and education to improve accuracy and reliability of the ergonomic assessment methods.

Human Papillomavirus Prevalence and Genotype Distribution in Normal and ASCUS Specimens: Comparison of a Reverse Blot Hybridization Assay with a DNA Chip Test

  • Kim, Sunghyun;Lee, In-soo;Lee, Dongsup
    • Biomedical Science Letters
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    • v.21 no.1
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    • pp.32-39
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    • 2015
  • High-risk (HR) human papillomavirus (HPV) genotypes are strongly associated with cervical cancer, whereas other HPV genotypes are not. To identify the various HPV genotypes in clinical samples, we conducted HPV genotyping using a DNA chip test and reverse blot hybridization assay (REBA) in normal cytology samples and atypical squamous cells of undetermined significance (ASCUS) cytology samples. We also investigated the HPV infection rate and HPV genotype prevalence in women with normal cytology and ASCUS cytology. Liquid-based cytology preparations were used for the initial screening of 205 subjects with normal cytology and ASCUS cytology. The HPV infection rate was 49.8% when using the DNA chip assay and 61.0% when using the REBA test. In patients with normal cytology, the HR-HPV positive rate was 21.9% with the DNA chip assay and 43.9% with the REBA test. In contrast, 8.3% of patients with ASCUS were HR-HPV positive when using the DNA chip assay, and 13.6% were positive when tested with the REBA test. The infection rate of HR-HPV in the 40~50-year age group was significantly higher than that of the other age groups. Based on the cytological analysis of the normal and ASCUS samples, the five most prominent HPV genotypes were HPV 16, 18, 68, 33, and 58 using the DNA chip test, and they were HPV 16, 18, 53, 33, and 66 when using the REBA test. In conclusion, the findings show that the results of the REBA test are comparable to those of the DNA chip test. Most strikingly, the REBA test detected the HR-HPV genotype associated with cervical carcinoma similar to that detected with the DNA chip method. Therefore, the REBA test is a useful method to detect clinically important HR-HPV genotypes.

Sequencing and annotation of the complete mitochondrial genome of a threatened labeonine fish, Cirrhinus reba

  • Islam, Mohammad Nazrul;Sultana, Shirin;Alam, Md. Jobaidul
    • Genomics & Informatics
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    • v.18 no.3
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    • pp.32.1-32.7
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    • 2020
  • The mitochondrial genome of a species is an essential resource for its effective conservation and phylogenetic studies. In this article, we present sequencing and characterization of the complete mitochondrial genome of a threatened labeonine fish, Cirrhinus reba collected from Khulna region of Bangladesh. The complete mitochondrial genome was 16,597 bp in size, which formed a circular double-stranded DNA molecule containing a total of 37 mitochondrial genes (13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes) with two non-coding regions, an origin of light strand replication (OL) and a displacement loop (D-loop), similar structure with other fishes of Teleostei. The phylogenetic tree demonstrated its close relationship with labeonine fishes. The complete mitogenome of Cirrhinus reba (GenBank no. MN862482) showed 99.96% identity to another haplotype of Cirrhinus reba (AP013325), followed by 90.18% identity with Labeo bata (AP011198).

Phase transformation of $REBa_2Cu_3O_{7-x}$ (RE=Nd, Gd, Dy) Superconductor during Continuous Cooling and Isothermal Heat Treatment (등온열처리와 냉각에 따른 $REBa_2Cu_3O_{7-x}$ (RE=Nd, Gd, Dy) 초전도체의 상변화)

  • O, Yong-Taek;Shin, Dong-Chan;Han, Young-Hee;Sung, Tae-Hyun;Jeong, Nyeon-Ho
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2003.05a
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    • pp.42-45
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    • 2003
  • The phase transformation of $REBa_2Cu_3O_{7-x}$ (RE=Nd, Gd, Dy) was investigated using isothermal heat-treatment and continuous cooling in air. During continuous cooling, the $REBa_2Cu_3O_{7-x}$ (RE=123) superconducting phase with well-distributed $REBa_2Cu_3O_{7-x}$ (RE-211) was obtainde at a cooling rate of $0.001^{\circ}C$/s. Single phase RE-123 (Nd, Gd, Dy) was stable at $1050^{\circ}C$, $1050^{\circ}C$, and $950^{\circ}C$ during isothermal heat-treatment, respectively. Above these temperatures the RE-211 phase existed within the RE-123 grains. The RE-123, RE-211, $BaCu_2Od_2$, and CuO phases coexisted at $50^{\circ}C$ below the partial melting temperature for each respective rare-earth RE-123.

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Review and Comparison of OWAS, RULA and REBA Based on Literature Survey (문헌조사에 기반한 OWAS, RULA 및 REBA의 연구 현황 및 비교)

  • Kee, Dohyung
    • Journal of the Korea Safety Management & Science
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    • v.23 no.2
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    • pp.31-36
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    • 2021
  • This study aims to review observational methods for assessing postural loads such as OWAS, RULA and REBA, and to compare them, based on the literature survey. The literature was searched through academic database of ScienceDirect using the key words of observational methods, OWAS, RULA and REBA. The results exhibited that of the thee methods, RULA was cited in the literature and applied to manufacturing industries the most frequently. Although it has been known that RULA is appropriate for assessing upper body postures, it has been applied to healthcare and social work activities, agriculture, forestry, fishing, construction, mining and quarrying, which require unstable lower limb postures. The countries where more number of relevant studies have been carried out were USA, India, Brazil, UK, etc. It was recommended that of the three techniques, RULA may be better for assessing postural loads, because it evaluated postural loads more highly, irrespective of industry, work type and lower limb postures, and its assessment results had higher agreement rate with experts' assessments than those of OWAS and REBA. It is expected that the results of this study will be used as a guideline for selecting an appropriate observational method.

Evaluation of a PCR-Reverse Blot Hybridization Assay to Identify Six Dermatophytes Predominant in the Republic of Korea

  • Jin, Hyunwoo;Kim, Hyunjung;Kim, Sunghyun;Choi, Yeonim;Bang, Hyeeun;Park, Sangjung;Wang, Hyeyoung;Lee, Jang-Ho;Jang, In Ho;Kim, Young-Kwon;Lee, Hyeyoung
    • Biomedical Science Letters
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    • v.20 no.3
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    • pp.139-146
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    • 2014
  • Accurate and rapid diagnosis of dermatophytosis, a disease whose prevalence has been steadily increased, is important for successful treatment. Current laboratory methods for diagnosing dermatophytosis rely on KOH mount and fungal culture method. However, these methods have low sensitivity and are time-consuming (2~4 weeks to diagnosis). In our previous study, a rapid molecular diagnostic assay (PCR-reverse blot hybridization assay, REBA) was developed to identify the following 6 main species of dermatophytes: Trichophyton rubrum, T. mentagrophytes, T. tonsurans, Microsporum canis, M. gypseum, and Epidermophyton floccosum. However, the REBA required more evaluation to validate its use in clinical examinations. The aim of the present study was to evaluate and validate the ability of the PCR-REBA to successfully identify dermatophytes in clinical isolates from dermatophytosis patients. Both conventional identification methods and the PCR-REBA were used to assess the presence of species of dermatophytes in 148 clinical isolates. The results of the two approaches were compared, and discrepancies between the two approaches were resolved by fungal ITS1 sequence analysis. T. rubrum was the most prevalent dermatophyte identified by conventional identification methods (118/148, 79.7%) and the PCR-REBA (131/148, 88.4%). The overall rate of consistency between conventional identification methods and the PCR-REBA was 79.0% (117/148 samples). Fungal ITS1 sequence analysis showed that PCR-REBA results were correct for 93.5% (29/31) of the discrepant samples. The PCR-REBA is rapid, sensitive, and highly specific compared with conventional identification methods. Thus, the PCR-REBA is a potentially useful tool for identifying dermatophytes in clinical settings.

Detection of Waterborne Pathogens in Public Bath Houses by PCR-Reverse Blot Hybridization Assay (PCR-REBA) (분자생물학적 방법인 PCR-REBA를 이용한 대중목욕탕 수질 중 수인성병원성미생물 검출)

  • Song, Woon-Heung;Choi, Seung-Gu;Yang, Byoung-Seon;Lee, Jae-Sang
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.12 no.8
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    • pp.3517-3522
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    • 2011
  • Contamination of public bath water by waterborne pathogens can cause disease outbreaks and contribute to background rates of disease. The aim of this study is to determine the prevalence of waterborne pathogens in public baths. A total of 30 water samples were collected from 30 different public baths in seoul, Korea. Pathogens in water samples were concentrated by 0.45 ${\mu}m$ nitrocellulose membrane filter, analyzed by both cultivation and polymerase chain reaction-reverse blot hybridization (PCR-REBA) of partial 16S rRNA gene. Various microorganisms including Escherichia coli and Shigella spp. were identified by microbiological cultivation. E. coli, Shigella spp., Salmonella spp., Pseudomonas spp. and Mycobacterium spp. were identified by PCR-REBA. Our results suggest that appropriate hygiene practice and continuous monitoring is needed for reducing health risk associated with public bath houses.

Stock identification of minor carp, Cirrhinus reba, Hamilton 1822 through landmark-based morphometric and meristic variations

  • Ethin, Rokhsana;Hossain, Md Shakhawate;Roy, Animesh;Rutegwa, Marcellin
    • Fisheries and Aquatic Sciences
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    • v.22 no.6
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    • pp.12.1-12.8
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    • 2019
  • Background: Wild fish populations stock is continuously diminishing in the Indo-Ganges river basin, and the population status of most fishes is unidentified. The identification of the population status and the conservation of commercially important and endemic wild fish populations in this region are crucial for the management. The aim of this paper was to identify the population status of Cirrhinus reba, a promising aquaculture but vulnerable species in the Indo-Ganges river basin in Bangladesh. Methods: C. reba samples were collected from four isolated populations of the Brahmaputra (n = 30), the Padma (33), the Karatoya (31), and the Jamuna Rivers (30) in Bangladesh, and the population status was evaluated using morphometric and landmark comparisons. Data were analyzed with the Kruskal-Wallis test, univariate analysis, discriminant function analysis, and the formation of a dendrogram. Results: Three meristic characters (Pectoral fin rays, caudal fin rays, scale in lateral lines), four morphometric characters (head length, pre-orbital length, post-orbital length, maximum body depth), and truss measurement (4-7) were significantly different among the stocks. The step-wise discriminant function analysis retained 15 variables from morphometric and landmark measurements that significantly differentiated the populations based on the constructed DFI and DFII. Discriminate function analysis also showed that 91.2% of the original groups were classified into their correct samples. The cluster analysis of Euclidean distances placed the Jamuna population in one cluster and the Brahmaputra, the Padma, and the Karatoya populations in the second one. Conclusion : Morphological differences among the stock were probably due to different ancestral origin. This is the first report about population status of C. reba in their natural habitat of the Indian subcontinent. Further genetic studies and the evaluation of environmental impact on C. reba populations in Bangladesh are suggested to support our findings.