• Title, Summary, Keyword: Region-specific

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Neural Network Modeling for the Superheated, Saturated and Compressed Region of Steam Table (증기표의 과열, 포화 및 압축영역의 신경회로망 모델링)

  • Lee, Tae-Hwan;Park, Jin-Hyun
    • Journal of the Korean Society of Mechanical Technology
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    • v.20 no.6
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    • pp.872-878
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    • 2018
  • Steam tables including superheated, saturated and compressed region were simultaneously modeled using the neural networks. Pressure and temperature were used as two inputs for superheated and compressed region. On the other hand Pressure and dryness fraction were two inputs for saturated region. The outputs were specific volume, specific enthalpy and specific entropy. The neural network model were compared with the linear interpolation model in terms of the percentage relative errors. The criterion of judgement was selected with the percentage relative error of 1%. In conclusion the neural networks showed better results than the interpolation method for all data of superheated and compressed region and specific volume of saturated region, but similar for specific enthalpy and entropy of saturated region.

Low Specific On-resistance SOI LDMOS Device with P+P-top Layer in the Drift Region

  • Yao, Jia-Fei;Guo, Yu-Feng;Xu, Guang-Ming;Hua, Ting-Ting;Lin, Hong;Xiao, Jian
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.14 no.5
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    • pp.673-681
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    • 2014
  • In this paper, a novel low specific on-resistance SOI LDMOS Device with P+P-top layer in the drift region is proposed and investigated using a two dimensional device simulator, MEDICI. The structure is characterized by a heavily-doped $P^+$ region which is connected to the P-top layer in the drift region. The $P^+$ region can modulates the surface electric field profile, increases the drift doping concentration and reduces the sensitivity of the breakdown voltage on the geometry parameters. Compared to the conventional D-RESURF device, a 25.8% decrease in specific on-resistance and a 48.2% increase in figure of merit can be obtained in the novel device. Furthermore, the novel $P^+P$-top device also present cost efficiency due to the fact that the $P^+$ region can be fabricated together with the P-type body contact region without any additional mask.

Molecular Characterization of the Region Encoding Integrative Functions from Enterococcal Bacteriophage ${\phi}$FC1

  • Kim, Min-Jung;Lee, Jin-Young;Kim, Young-Woo;Sung, Ha-Chin;Chang, Hyo-Ihl
    • BMB Reports
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    • v.29 no.5
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    • pp.448-454
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    • 1996
  • Bacteriophage ${\phi}FC1$ is a temperate phage which was identified as a prophage in the Enterococcus faecalis KBL703 chromosome. Phage ${\phi}FC1$ integrates into the host chromosome by site-specific recombination. The phage attachment site P (attP) was localized within the 0.65-kb XhoI-HindIII fragment and the nucleotide sequence of the region was determined. An open reading frame (mj1) which adjoined the phage attachment site encoded a deduced protein related to the site-specific recombinase family. The organization of this region was comparable to other site-specific recombination systems. The molecular weight of the expressed MJ1 in E. coli was in good agreement with the predicted 53,537 Da of the mj1 gene product. Elucidation of the phage-specific integration process in this study would provide useful genetic tools such as a chromosomal integration system.

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Identification of an Enhancer Critical for the ephirn-A5 Gene Expression in the Posterior Region of the Mesencephalon

  • Park, Eunjeong;Noh, Hyuna;Park, Soochul
    • Molecules and Cells
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    • v.40 no.6
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    • pp.426-433
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    • 2017
  • Ephrin-A5 has been implicated in the regulation of brain morphogenesis and axon pathfinding. In this study, we used bacterial homologous recombination to express a LacZ reporter in various ephrin-A5 BAC clones to identify elements that regulate ephrin-A5 gene expression during mesencephalon development. We found that there is mesencephalon-specific enhancer activity localized to a specific +25.0 kb to +30.5 kb genomic region in the first intron of ephrin-A5. Further comparative genomic analysis indicated that two evolutionary conserved regions, ECR1 and ECR2, were present within this 5.5 kb region. Deletion of ECR1 from the enhancer resulted in disrupted mesencephalon-specific enhancer activity in transgenic embryos. We also found a consensus binding site for basic helix-loop-helix (bHLH) transcription factors (TFs) in a highly conserved region at the 3'-end of ECR1. We further demonstrated that specific deletion of the bHLH TF binding site abrogated the mesencephalon-specific enhancer activity in transgenic embryos. Finally, both electrophoretic mobility shift assay and luciferase-based transactivation assay revealed that the transcription factor Ascl1 bound the bHLH consensus binding site in the mesencephalon-specific ephrin-A5 enhancer in vitro. Together, these results suggest that the bHLH TF binding site in ECR1 is involved in the positive regulation of ephrin-A5 gene expression during the development of the mesencephalon.

Human Y Chromosome: Structure, Function and Evolution (인간 Y 염색체: 구조, 기능 그리고 진화)

  • 홍경원;허재원;김희수
    • Journal of Life Science
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    • v.13 no.6
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    • pp.958-969
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    • 2003
  • The human Y chromosome is strictly paternally inherited and does not X-Y crossing over during male meiosis in most of its length. Although this region came to be known as the non-recombining region Y (NRY), it was renamed as male-specific region Y (MSY) due to abundant recombination. The MSY is a mosaic of heterochromatic sequences and three classes of euchromatic sequences: X-transposed, X-degenerated and ampliconic. The X-transposed sequences exhibit 99% identity to the X chromosomal sequences. The X-degenerate sequences are remnants of ancient autosomes from which the modem X and Y chromosomes evolved. Eight palindromes of the ampliconic comprise one-quarter of the euchromatic DNA of the male-specific region of the human Y chromosome. They contain many testis-specific genes and typically exhibit 99.97% intra-palindromic (arm-to-arm) sequence identity. The arms of these palindromes must have subsequently engaged in gene conversion, driving the pair arms to evolve it concert. Averages of approximately 600 nucleotides per newborn male have undergone Y-Y gene conversion, which has had an important role in the evolution of multi-copy testis gene families in the MSY.

Investigation on the Feasible Range of Design Variables Versus Specific Speed for Centrifugl Pumps (원심 펌프의 설계를 위한 비속도에 따른 무차원 설계 변수의 설계 범위에 관한 연구)

  • Park, Moo-Ryong;Oh, Jae-Min;Yoo, Il-Su
    • 유체기계공업학회:학술대회논문집
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    • pp.655-660
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    • 2005
  • The feasible range of design variables for centrifugal pumps have been investigated. The present study has suggested a searching procedure to find the feasible ranges using only non-dimensional parameters. The results in the typical specific speed region and the low specific speed region have been presented.

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Identification of the Regulatory Region Responsible for Vascular Tissue-Specific Expression in the Rice Hd3a Promoter

  • Pasriga, Richa;Cho, Lae-Hyeon;Yoon, Jinmi;An, Gynheung
    • Molecules and Cells
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    • v.41 no.4
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    • pp.342-350
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    • 2018
  • Flowering time is determined by florigens. These genes include, Heading date 3a (Hd3a) and Rice FT 1 (RFT1) in rice, which are specifically expressed in the vascular tissues of leaves at the floral transition stage. To study the cis-regulatory elements present in the promoter region of Hd3a, we generated transgenic plants carrying the 1.75-kb promoter fragment of Hd3a that was fused to the ${\beta}$-glucuronidase (GUS) reporter gene. Plants expressing this construct conferred a vascular cell-specific expression pattern for the reporter gene. However, GUS was expressed in leaves at all developmental stages, including the early seedling stage when Hd3a was not detected. Furthermore, the reporter was expressed in roots at all stages. This suggests that the 1.75-kb region lackings cis-elements that regulate leaf-specific expression at the appropriate developmental stages. Deletion analyses of the promoter region indicated that regulatory elements determining vascular cell-specific expression are present in the 200-bp region between -245 bp and -45 bp from the transcription initiation site. By transforming the Hd3a-GUS construct to rice cultivar 'Taichung 65' which is defective in Ehd1, we observed that Ehd1 is the major regulatory element that controls Hd3a promoter activity.

Sequence Variations in the Non-Coding Sequence of CTX Phages in Vibrio cholerae

  • Kim, Eun Jin;Yu, Hyun Jin;Kim, Dong Wook
    • Journal of Microbiology and Biotechnology
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    • v.26 no.8
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    • pp.1473-1480
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    • 2016
  • This study focused on the variations in the non-coding sequences between ctxB and rstR of various CTX phages. The non-coding sequences of CTX-1 and CTX-cla are phage type-specific. The length of the non-coding region of CTX-1 and CTX-cla is 601 and 730 nucleotides, respectively. The non-coding sequence of CTX phage could be divided into three regions. There is a phage type-specific Variable region between two homologous Common regions (Common regions 1 and 2). The non-coding sequence of RS1 element is similar to CTX-1 except that Common region 1 is replaced by a short RS1-specific sequence. The non-coding sequences of CTX-2 and CTX-cla are homologous, indicating the non-coding sequence of CTX-2 is derived from CTX-cla. The non-coding region of CTX-O139 is similar to CTX-cla and CTX-2; however, it contains an extra phage type-specific sequence between Common region 2 and rstR. The variations in the non-coding sequences of CTX phages might be associated with the difference in the replication efficiency and the directionality in the integration into the V. cholerae chromosome.

A New SOI LDMOSFET Structure with a Trench in the Drift Region for a PDP Scan Driver IC

  • Son, Won-So;Kim, Sang-Gi;Sohn, Young-Ho;Choi, Sie-Young
    • ETRI Journal
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    • v.26 no.1
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    • pp.7-13
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    • 2004
  • To improve the characteristics of breakdown voltage and specific on-resistance, we propose a new structure for a LDMOSFET for a PDP scan driver IC based on silicon-on-insulator with a trench under the gate in the drift region. The trench reduces the electric field at the silicon surface under the gate edge in the drift region when the concentration of the drift region is high, and thereby increases the breakdown voltage and reduces the specific on-resistance. The breakdown voltage and the specific on-resistance of the fabricated device is 352 V and $18.8 m{\Omega}{\cdot}cm^2$ with a threshold voltage of 1.0 V. The breakdown voltage of the device in the on-state is over 200 V and the saturation current at $V_{gs}=5V$ and $V_{ds}$=20V is 16 mA with a gate width of $150{\mu}m$.

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Function of mORF1 Protein as a Terminal Recognition Factor for the Linear Mitochondrial Plasmid pMLP1 from Pleurotus ostreatus

  • Kim, Eun-Kyoung;Roe, Jung-Hye
    • Journal of Microbiology
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    • v.37 no.4
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    • pp.229-233
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    • 1999
  • The mitochondrial plasmid pMLP1 from a white-rot fungus, Pleurotus ostreatus, is a double-stranded DNA containing 381 bp terminal inverted repeat (TIR) whose 5'-ends are covalently bound by terminal proteins. The plasmid contains two major open reading frames (ORFs), encoding putative DNA and RNA polymerases, and a minor ORF encoding a small, highly basic protein. To identify the DNA binding activity that recognizes the TIR region of pMLP1, gel retardation assays were performed with mitochondrial extracts. A specific protein binding to a region between 123 and 248 nt within TIR was observed. We examined whether the gene product of mORF1 bindes to this region specifically. E. coli cell extract which contains an overproduced mORF1 protein formed a complex specific to the region between 123 and 248 nt. Inclusion of mORF1 protein in the specific complex formed between P. ostreatus mitochondrial extract and TIR was confirmed by a supershift assay using polyclonal antibodies against the mORF1 protein. Our result suggest that the product of mORF1 may function as a terminal region recognition factor (TRF), recognizing an internal region in TIR.

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