• Title, Summary, Keyword: Ruta graveolens L.

Search Result 2, Processing Time 0.035 seconds

Isolation and Identification of Active Antimicrobial Substance against Listeria monocytogenes from Ruta graveolens Linne (운향으로부터 Listeria monocytogenes에 대한 항균 활성 물질의 분리 및 구조동정)

  • Ahn, Yong-Seon;Shin, Dong-Hwa;Baek, Nam-In
    • Korean Journal of Food Science and Technology
    • /
    • v.32 no.6
    • /
    • pp.1379-1388
    • /
    • 2000
  • Ethanol extracts from Ruta graveolens Linne exhibited strong antimicrobial activities by disc diffusion method against 5 strains of Listeria monocytogenes (ATCC 19111, ATCC 19112, ATCC 19113, ATCC 19114 and ATCC 15313). Ethanol extract from Ruta graveolens Linne was subsequently fractionated by n-hexane, chloroform, ethyl acetate and water. Chloroform fraction of Ruta graveolens Linne showed strong growth inhibition at concentrations as low as 40 ppm level in broth culture medium against 5 strains of L. monocytogenes for 72 hr at $30^{\circ}C$. Single substance(RTG1-1) was isolated by silica gel column chromatography from chloroform fraction of Ruta graveolens Linne. RTG1-1 showed a strong bactericidal activity against L. monocytogenes at a concentration of 20 ppm level. Purified RTG1-1 was identified as gravacridonechlorine by analyses of EI-Mass, $^1H-NMR$ and $^{13}C-NMR$.

  • PDF

Inhibition against Helicobacter pylori and Biological Activities by Rue (Ruta graveolens L.) Extracts (Rue(Ruta graveolens L.) 추출물의 Helicobacter pylori에 대한 항균활성과 생리활성효과)

  • Cho, Young-Je;Chun, Sung-Sook;Kim, Jeung-Hoan;Yoon, So-Jung
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.34 no.4
    • /
    • pp.460-465
    • /
    • 2005
  • Water and ethanol extracts from Rue were prepared, and their growth inhibiting activity against Helicobacter pylori and other biological activities were examined. Total phenolic compounds in the water and ethanol extracts were present at the concentration of 16.39 mg/g, and 17.07 mg/g, respectively. At the concentration of $200\;{\mu}g/mL$ of phenolic compounds concentration, water extract produced 12 mm inhibition zone while ethanol extract produced 13 mm. The ABTS [2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)] radical decolorization and antioxidant protection factor (PF) were determined for extracts from Rue. Water extracts showed $96\%$ inhibition rate on ABTS, but ethanol extracts showed higher PF (1.2) than water extracts (0.8). Water extracts had higher electron donation ability on DPPH than ethanol extracts. But ethanol extracts had higher ACE (angiotensin converting enzyme) and xanthine oxidase inhibitory activities than water extracts. Rosemarinic acid and quercetin were the most abundant phenolic compounds as analyzed by HPLC.