• Title, Summary, Keyword: S. epidermidis

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Root canal irrigants influence the hydrophobicity and adherence of Staphylococcus epidermidis to root canal dentin: an in vitro study

  • Nagendrababu, Venkateshbabu;Sultan, Omer Sheriff;Kannathasan, Sreedharan;Patel, Amir Shahreza;Chitra, Ebenezer;Neelakantan, Prasanna;Davamani, Fabian
    • Restorative Dentistry and Endodontics
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    • v.43 no.1
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    • pp.1.1-1.8
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    • 2018
  • Objectives: To determine the effect of root canal irrigants on the hydrophobicity and adherence of Staphylococcus epidermidis (S. epidermidis) to root canal dentin in vitro. Materials and Methods: Root dentin blocks (n = 60) were randomly divided into 4 groups based on the irrigation regimen: group 1, saline; group 2, 5.25% sodium hypochlorite (NaOCl); group 3, 5.25% NaOCl followed by 17% ethylenediaminetetraacetic acid (EDTA); group 4, same as group 3 followed by 2% chlorhexidine (CHX). The hydrophobicity of S. epidermidis to root dentin was calculated by cell surface hydrophobicity while the adherence was observed by fluorescence microscopy, and bacteria were quantified using ImageJ software (National Institutes of Health). Statistical analysis of the data was done using Kruskal-Wallis test and Mann-Whitney U test (p = 0.05). Results: The hydrophobicity and adherence of S. epidermidis to dentin were significantly increased after irrigating with group 3 (NaOCl-EDTA) (p < 0.05), whereas in group 4 (NaOCl-EDTA-CHX) both hydrophobicity and adherence were significantly reduced (p < 0.05). Conclusions: The adherence of S. epidermidis to dentin was influenced differently by root canal irrigants. Final irrigation with CHX reduces the bacterial adherence and may impact biofilm formation.

In vitro evaluation of octenidine as an antimicrobial agent against Staphylococcus epidermidis in disinfecting the root canal system

  • Chum, Jia Da;Lim, Darryl Jun Zhi;Sheriff, Sultan Omer;Pulikkotil, Shaju Jacob;Suresh, Anand;Davamani, Fabian
    • Restorative Dentistry and Endodontics
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    • v.44 no.1
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    • pp.8.1-8.7
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    • 2019
  • Objectives: Irrigants are imperative in endodontic therapy for the elimination of pathogens from the infected root canal. The present study compared the antimicrobial efficacy of octenidine dihydrochloride (OCT) with chlorhexidine (CHX) and sodium hypochlorite (NaOCl) against Staphylococcus epidermidis (S. epidermidis) for root canal disinfection. Materials and Methods: The minimum inhibitory concentration (MIC) was obtained using serial dilution method. The agar diffusion method was then used to determine the zones of inhibition for each irrigant. Lastly, forty 6-mm dentin blocks were prepared from human mandibular premolars and inoculated with S. epidermidis. Samples were randomly divided into 4 groups of 10 blocks and irrigated for 3 minutes with saline (control), 2% CHX, 3% NaOCl, or 0.1% OCT. Dentin samples were then collected immediately for microbial analysis, including an analysis of colony-forming units (CFUs). Results: The MICs of each tested irrigant were 0.05% for CHX, 0.25% for NaOCl, and 0.0125% for OCT. All tested irrigants showed concentration-dependent increase in zones of inhibition, and 3% NaOCl showed the largest zone of inhibition amongst all tested irrigants (p < 0.05). There were no significant differences among the CFU measurements of 2% CHX, 3% NaOCl, and 0.1% OCT showing complete elimination of S. epidermidis in all samples. Conclusions: This study showed that OCT was comparable to or even more effective than CHX and NaOCl, demonstrating antimicrobial activity at low concentrations against S. epidermidis.

The Experimental Study on Anti-bacterial Potency of Soyum Herbal-acupuncture, Hwangryunhaedoktang & Coptidis rhizoma on Staphylococcus epidermidis (소염 약침액, 황련해독탕, 황련이 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구)

  • Seo, Hyung-Sik
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.19 no.2
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    • pp.19-25
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    • 2006
  • Objective : This experimental study was performed to investigate the effect of Soyum Herbal-acupuncture, Hwangryunhaedoktang & Coptidis rhizoma on Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by dropping to $50{\mu}l$ according to density Soyum Herbal-acupuncture, Hwangryunhaedoktang & Coptidis rhizoma(100%, 10%, 1%, 0.1%). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on Staphylococcus epidermidis in Soyum Herbal-acupuncture dippear all sample. 2. MIC on Staphylococcus epidermidis in Hwangryunhaedoktang disappear all sample. 3. MIC on Staphylococcus epidermidis in Coptidis rhizoma was 100%, $50{\mu}l$ and anti-bacterial potency increase compare with quantity. Conclusions : The present author think that Soyum Herbal-acupuncture must be extracted a new method for anti-bacterial potency and Coptidis rhizoma was made Herbal-acupuncture for eye drops.

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A Case of Pyogenic Liver Abscess Caused by Staphylococcus epidermidis in a Healthy Child (건강한 소아에서 발생한 Staphylococcus epidermidis에 의한 화농성 간농양 1례)

  • Gwak, Ji Hye;Eem, Yeun Joo;Choi, Ui Yoon;Kang, Jin Han
    • Pediatric Infection and Vaccine
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    • v.20 no.1
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    • pp.36-40
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    • 2013
  • Staphylococcus epidermidis is a normal inhabitant of skin, throat, mouth, vagina, and urethra. It is not usually pathogenic, particularly in immunocompetent hosts. This report describes a case of a pyogenic liver abscess caused by Staphylococcus epidermidis in a healthy 12-year-old boy. He was admitted to Seoul St. Mary's Hospital with intermittent fever for 6 days. Findings on abdominal computed tomography (CT) showed a mass measuring $7.0{\times}6.5$ cm in the right hepatic lobe. Culture of the abscess resulted in growth of Staphylococcus epidermidis as a causative organism. The patient was successfully treated with intravenous administration of antibiotics and percutaneous drainage of the abscess.

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Fabrication of Antimicrobial Wound Dressings Using Silver-Citrate Nanorods and Analysis of Their Wound-Healing Efficacy

  • Park, Yong Jin;Jeong, Jisu;Kim, Jae Seok;Choi, Dong Soo;Cho, Goang-Won;Park, Jin Seong;Lim, Jong Kuk
    • Journal of the Chosun Natural Science
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    • v.12 no.2
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    • pp.47-57
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    • 2019
  • Staphylococcus epidermidis is well-known not only as an innocuous normal flora species commonly isolated from human skin, but also as an important bacterial species to keep skin healthy, because this species can protect the human skin from pathogenic microorganisms. However, S. epidermidis turns into a potential pathogen in damaged skin, because these bacteria can easily form a biofilm on the wound area and provide antimicrobial resistance to other microorganisms embedded in the biofilm. Thus, it is important to kill S. epidermidis in the early stage of wound treatment and block the formation of biofilms in advance. In the present study, hydrogel wound dressings were fabricated using polyvinyl alcohol/polyethylene glycol containing silver citrate nanorods, which have been proven to have strong antimicrobial activity, especially against S. epidermidis, and their wound-healing efficacy was investigated in vivo using a rat experiment.

The Experimental Study on Anti-bacterial Potency of Hwangryunhaedoktang Herbal-acupuncture & extract on Staphylococcus aureus & Staphylococcus epidermidis (황련해독탕 약침액과 전탕액이 Staphylococcus aureus와 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구)

  • Seo, Hyung-Sik
    • Journal of Pharmacopuncture
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    • v.9 no.2
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    • pp.99-103
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    • 2006
  • Objective : This experimental study was performed to investigate the effect of Hwangryunhaedoktang Herbal-acupuncture & extract on Staphylococcus aureus and Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by dropping to $50{\beta}^{\circ}$ according to density Hwangryunhaedoktang Herbal-acupuncture & extract(100%, 10%, 1%, 0.1%). Anti-bacterial potency was measured by the size of inhibition zone With change of volume. Results : 1. MIC on Staphylococcus aureus in Hwangryunhaedoktang Herbal-acupuncture & extract were not disappear all sample. 2. MIC on Staphylococcus epidermidis in Hwangryunhaedoktang Herbal-acupuncture & extract were not disappear all sample. Conclusions : The present author think that Hwangryunhaedoktang Herbal-acupuncture must be extract a new method for anti-bacterial potency.

The Experimental Study on Anti-bacterial Potency of Soyum Herbal-acupuncture on Staphylococcus aureus & Staphylococcus epidermidis (소염 약침액이 Staphylococcus aureus와 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구)

  • Seo, Hyung-Sik
    • Journal of Pharmacopuncture
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    • v.9 no.2
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    • pp.73-77
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    • 2006
  • Objective : This experimental study was performed to investigate the effect of Soyum Herbal-acupuncture on Staphylococcus aureus and Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by dropping to $50\;{\mu}l$ according to density Soyum Herbal-acupuncture(100%, 10%, 1%, 0.1%). Antibacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on Staphylococcus aureus in Soyum Herbal-acupuncture disappear all sample. 2. MIC on Staphylococcus epidermidis in Soyum Herbal-acupuncture disappear all sample. Conclusions : The present author think that Soyum Herbal-acupuncture must be extract a new method for anti-bacterial potency.

The Experimental Study on Anti-bacterial Potency of Coptidis rhizoma extract on Staphylococcus aureus & Staphylococcus epidermidis (황련 전탕액이 Staphylococcus aureus와 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구)

  • Seo, Hyung-Sik
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.19 no.2
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    • pp.71-76
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    • 2006
  • Objective : This experimental study was performed to investigate the effect of Coptidis rhizoma extract on Staphylococcus aureus and Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by flowing to $50{\mu}l$ according to density Coptidis rhizoma extract(100%, 10%, 1%, 0.1%). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on Staphylococcus aureus in Coptidis rhizoma extract was 100%, $50{\mu}l$ and anti-bacterial potency increase compare with quantity. 2. MIC on Staphylococcus epidermidis in Coptidis rhizoma extract was 100% & 10%, $50{\mu}l$ and anti-bacterial potency increase compare with quantity. Conclusions : The Present author think that Coptidis rhizoma extract must be made a new eye drops.

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Extensive Bilateral Lemierre Syndrome due to Methicillin-Resistant Staphylococcus epidermidis in a Patient with Lung Adenocarcinoma

  • Choi, Bo Mi;Son, Seong Wan;Park, Chan Kwon;Lee, Sang-Hoon;Yoon, Hyung Kyu
    • Tuberculosis and Respiratory Diseases
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    • v.78 no.3
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    • pp.289-292
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    • 2015
  • Lemierre syndrome (LS) is a septic thrombophlebitis of the internal jugular vein (IJV) following an oropharyngeal infection. LS is commonly caused by normal anaerobic flora and treated with appropriate antibiotics and anticoagulation therapy. Although the incidence of disease is very rare, 15% cases of LS are fatal even in the antibiotic era because of disseminated septic thromboemboli. We reported a case of extensive bilateral LS due to methicillin-resistant Staphylococcus epidermidis in a 63-year-old female with lung adenocarcinoma. Initial examination revealed a retropharyngeal abscess; hence, intravenous ceftriaxone and steroid were initiated empirically. However, pulmonary thromboembolism developed and methicillin-resistant S. epidermidis was identified in the bacterial culture. Despite intensive antibiotic and anticoagulation therapies, extensive septic thrombophlebitis involving the bilateral IJV and superior vena cava developed. Adjunctive catheter-directed thrombolysis and superior vena cava stenting were performed and the patient received antibiotic therapy for an additional 4 weeks, resulting in complete recovery.

Molecular typing of epidemiologically unrelated Staphylococcus epidermidis recovered from dogs by pulsed-field gel electrophoresis

  • Pak, Son-il
    • Korean Journal of Veterinary Research
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    • v.39 no.4
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    • pp.811-818
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    • 1999
  • A total of 16 Staphylococcus epidermidis isolates collected from 14 dogs admitted to the Veterinary Medicial Teaching Hospital in Seoul National University over eleven months were examined for in vitro antibiotic susceptibility pattern with minimum inhibitory concentration (MIC) and slime production, a virulence-associated phenotype, and were genetically characterized by pulsed-field gel electrophoresis (PFGE). The frequency of resistance to antimicrobial agents tested was not high, with a susceptibility ranging from 56.3% to 100%. Three strains exhibited multiple drug resistance against amikacin (MIC, $32-64{\mu}g/ml$), ampicillin ($32{\mu}g/ml$), fosfomycin ($32-128{\mu}g/ml$) and gentamicin ($16{\mu}g/ml$). Vancomycin, ciprofloxacin and rifampin were effective antibiotics against the isolates. All isolates were slime producers ; strains isolated from dogs which died of bacteremia were more likely to produce slime than those isolated from dogs which survived. Chromosomal DNA fingerprinting of the isolates yielded 16 different genomic types with few common bands, indicating a variety of clones of S epidermidis were prevalent in the hospital. This study revealed that PFGE is an useful method for the genotype characterization of S epidermidis strains and this organism could probably be pathogenic in some dogs with severe disorders. Further works on a larger number of epidemiologically defined strains are required to assess these results.

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