• Title, Summary, Keyword: S. epidermidis

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Antimicrobial Effects of Photodynamic Therapy using Photofrin Against Staphylococcus aureus and Staphylococcus epidermidis (포토프린을 이용한 황색포도알균과 표피포도알균에 대한 광역학 치료의 항균효과)

  • Kwon, Pil-Seung
    • The Journal of the Korea Contents Association
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    • v.13 no.2
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    • pp.314-321
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    • 2013
  • Photodynamic therapy(PDT) has been recommended as an alternative therapy for various diseases including microbial infection. The aim of the present study is to evaluate the antimicrobial effect of PDT using a photofrin and home made 630 nm Light emitting diode(LED) against Staphylococci. To examine the antimicrobial effect of photofrin-mediated PDT against Staphylococcus aureus and Staphylococcus epidermidis colony forming units(CFU) quantification, and bacterial viability using flow cytometry were formed. The CFU quantification results of S. aureus and S. epidermidis were 1 cfu/ml and 16 cfu/ml of average, respectively, after PDT application with photofrin of $50{\mu}g/m{\ell}$ and 630 nm LED and energy density of $18J/cm^2$. In addition, S. aureus and S. epidermidis isolates yielded forward-scatter (FSC) and fluorescence intensity (FI) differences on flow cytometry (FCM) after PDT. S. aureus and S. epidermidis cell size(FSC) increased 8.96% and 5.55% respectively, after PDT. Also the numbers of dead cell of S. aureus and S. epidermidis were a 39% and 61% incerased. These results suggest that photofrin-mediated PDT can be an effective alternative treatment for antibacterial therapy.

Prevention of Catheter-related Infections (CRIs) using Ciprofloxacin

  • Jeon Sung Min;Kim Mal Nam
    • Biomedical Science Letters
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    • v.10 no.3
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    • pp.245-251
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    • 2004
  • Microbial infection provokes one of the most serious complications to the patients with indwelling catheters. Ciprofloxacin (CFX) was added into the catheter materials (polyurethane or silicone) during the manufacturing process to avoid the microbial infection. Efficacy of the catheters containing CFX was investigated by using the in vitro zone of growth inhibition test method. The catheters made of polyurethane or silicone exhibited a strong antimicrobial activity against the major catheter-related microorganisms (S. aureus, S. epidermidis, P. aeruginosa and E. coli), when CFX was incorporated into the catheters. Fetal bovine serum (FBS) did not affected antimicrobial activities of the polyurethane catheters with CFX loading of 0.5 and 1.0% (W/W) against S. aureus and S. epidermidis. However, the polyurethane catheters with 1.0% (W/W) of CFX loading showed a significantly (P<0.05) reduced antimicrobial activity against E. coli when the catheters were exposed to FBS. Silicone catheters with 1.0 and 1.5% (W/W) of CFX loading demonstrated effective antimicrobial activity against S. epidermidis for at least 2 weeks. These results suggest that the use of catheters containing ciprofloxacin could be effective in preventing catheter-related infections.

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Changes in Blood Parameters of the Cultured Flounder Paralichthys olivaceus Artificially Infected with Staphylococcus epidermidis (Staphylococcus epidermidis로 인위감염시킨 양식넙치의 혈액지수 변동)

  • Sim, Doo-Saing;Jung, Sung-Hee;Park, Hyung-Sook;Chun, She-Kyu
    • Journal of fish pathology
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    • v.6 no.2
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    • pp.123-131
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    • 1993
  • The cultured flounder(Paralichthys olivaceus) was injected with Staphylococcus epidermidis, various hematological and blood chemical changes were monitored over 96 hours. Red blood cell count, hemoglobin hematocrit. mean corpuscular hemoglobin concentration and mean corpuscular hemoglobin were significantly depressed after 24 to 48hours. Total protein, albumin, globulin and total cholestrol were significantly increased by the 24 or 48 hours, Glucose, bilirubin and transaminase were significantly depressed by 24 to 48hours. Erythrocytes were gotten shorter with round-shaped after 48hours inoculated with S. cpidermidis. Hemolytic erythrocytes and neutrophils were showed after 72hours inoculated with S. epidermidis. Price-Jones curve was transformed for left shift after 48hours inoculated with S. epidermidis, therfore staphylococcia appeared hemolytic anemia in the artificially infection.

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The Experimental Study on Anti-bacterial Potency of Variou Herb-medicine used for eye disease on Staphylococcus epidermidis Keratitis (다종의 안질환 사용 처방이 각막염 유발균인 Staphylococcus epidermidis에 미치는 영향)

  • Jung, Soon-Young;Seo, Hyung-Sik
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.19 no.1
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    • pp.123-134
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    • 2006
  • Objective : This experimental study was performed to investigate the effect of Herbe-medicine used for eye disease(Sean-tang, Jinpi-san, Tangpo-san, Copitdis Rhizoma) on Staphylococcus epidermidis keratitis. Methods : After administering various herbal eye drops on Staphylococcus epidermidis, I measured MIC and the size of inhibition zone. MIC was measured by dropping from 20 to $50{\mu}{\ell}$ according to density. Anti-bacterial potency was measured by the size of inhibition zone with change of volume under 2 days culture condition. Also continuous anti-bacterial potency of herbal eye drops was measured by the size of inhibition zone according to 2 days and 6 days culture each under the $50{\mu}{\ell}$ condition. Results : 1. MIC on Staphylococcus epidermidis in Sean-tang, Jinpi-san was 1%, $50{\mu}{\ell}$ 2. MIC on Staphylococcus epidermidis in Tangpo-san, Coptidis rhizoma was 10%, $50{\mu}{\ell}$ 3. MIC on Staphylococcus epidermidis in Cravit was 0.1%, $20{\mu}{\ell}$. 4. Under the 2 days culture condition, the size of inhibition zone on Staphylococcus epidermidis by volume for Sean-tang was 10.0mm in $50{\mu}{\ell}$, Jinpi-san was 16.0mm in $50{\mu}{\ell}$, Tangpo-san was 17.5mm in $50{\mu}{\ell}$, Coptidis rhizoma was 31.0mm in $50{\mu}{\ell}$ and Cravit was 34mm in $50{\mu}{\ell}$, showed the highest anti-bacterial potency. 5. Under the $50{\mu}{\ell}$ condition, the size of inhibition zone on Staphylococcus epidermidis by 2 and 6 culture days for Sean-tang was 47.5mm in 6days, Jinpi-san was 36.0mm in 6days, Tangpo-san was 45.0mm in 6 days and Cravit was 48.0mm in 6 days, which showed the highest anti-bacterial potency. 6. Under the $50{\mu}{\ell}$ culture condition, the size of inhibition zone on Staphylococcus epidermidis by 2 and 6 culture days for Coptidis rhizoma was 31.0mm in 2 days and 6 days, which showed the same anti-bacteria1 potency. Conclusions : The present author think that Herbe-medicine used for eye disease can be used to cure Staphylococcus epidermidis keratitis and if further study is performed, the use of the Herbe-medicine used for eye disease will be valuable and beneficial in the clinical medicines.

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Inactivation of S. epidermidis, B. subtilis, and E. coli Bacteria Bioaerosols Deposited on a Filter Utilizing Airborne Silver Nanoparticles

  • Lee, Byung-Uk;Yun, Sun-Hwa;Ji, Jun-Ho;Bae, Gwi-Nam
    • Journal of Microbiology and Biotechnology
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    • v.18 no.1
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    • pp.176-182
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    • 2008
  • In the present study, a control methodology utilizing airborne silver nanoparticles is suggested and tested with respect to its potential to control Gram-positive Staphylococcus epidermidis and Bacillus subtilis, and Gram-negative Escherichia coli bacteria bioaerosols deposited on filters. As it is known that the Gram-negative bacteria are sensitive to airflow exposure, the main focus of this study for testing the airborne silver nanoparticles effect was the Gram-positive Staphylococcus epidermidis and Bacillus subtilis bacteria bioaerosols whereas Escherichia coli bioaerosols were utilized for comparison. Airborne bacteria and airborne silver nanoparticles were quantitatively generated in an experimental system. Bioaerosols deposited on the filter were exposed to airborne silver nanoparticles. The physical and biological properties of the airborne bacteria and airborne silver nanoparticles were measured via aerosol measurement devices. From the experimental results, it was demonstrated that this method utilizing airborne silver nanoparticles offers potential as a bioaerosol control methodology.

Antimicrobial Activity of Medicinal Herbs against Salmonella gallinarum and Staphylococcus epidermidis (Salmonella gallinarum와 Staphylococcus epidermidis 균주에 대한 한약재의 항균 활성)

  • Choi, I.;Chang, H.S.
    • Korean Journal of Poultry Science
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    • v.36 no.3
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    • pp.231-238
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    • 2009
  • The present study was conducted to investigate the antimicrobial activities of extracts from approximately 40 different traditional Korean medicinal herbs against S. gallinarum and S. epidermidis. The extracts from Schizandra chinensis Baill., Melia azedarach Linn$\acute{e}$, Caesalpinia sappan Linn$\acute{e}$. and Rhus javanica Linn$\acute{e}$. exhibited high antimicrobial activities against S. gallinarum, whereas the extracts from Melia azedarach Linn$\acute{e}$ and Rhus javanica Linn$\acute{e}$. exhibited high antimicrobial growth for S. epidermidis. Minimum inhibitory concentrations (MIC) of Melia azedarach Linn$\acute{e}$, Caesalpinia sappan Linn$\acute{e}$. and Rhus javanica Linn$\acute{e}$. for S. gallinarum were 1.2 mg/mL, whereas MIC of exracts from Rhus javanica Linn$\acute{e}$. extract for S. epidermidis were 0.6 mg/mL. Heat treatment of the extracts from Schizandra chinensis Baill. and Rhus javanica Linn$\acute{e}$. caused a significant reduction in antimicrobial activities against S. gallinarum. but didn't affect antimicrobial activities against S. edidermidis. Alkaline treatment of the extracts from Schizandra chinensis Baill. caused a significant reduction in antimicrobial activities against S. gallinarum, while similar treatment of the extracts from Rhus javanica Linn$\acute{e}$. caused a significant increase in antimicrobial activities against S. edidermidis. Since extracts from Rhus javanica Linn$\acute{e}$. and Caesalpinia sappan Linn$\acute{e}$. exhibited the highest antimicrobial activities, these extracts at the concentrations of 100, 300 or 500 ppm were added and then bacterial growth-inhibiting activities for S. gallinarum and S. epidermidis by these two extracts were further examined. Optical density at 620 nm ($OD_{620}$) after 24 hours incubation in the absence of Rhus javanica Linn$\acute{e}$. extract ranged from 0.30 to 0.45 compared with $OD_{620}$ value ranging from 0.06 to 0.18 in the presence of 100, 300 or 500 ppm of the extract, indicating that growth of all bacteria was significantly inhibited within 24 hours by the addition of at least 100 ppm of Rhus javanica Linn$\acute{e}$ extract. Value of $OD_{620}$ after 24 hours incubation in the absence of Caesalpinia sappan Linn$\acute{e}$. extract ranged from 0.30 to 0.55 compared with $OD_{620}$ value ranging from 0.05 to 0.15 in the presence of 300 or 500 ppm of the extract, indicating that growth of all bacteria was also significantly inhibited within 24 hours by the addition of at least 300 ppm of Caesalpinia sappan Linn$\acute{e}$. extract. In conclusion, these findings suggest that extracts from Rhus javanica Linn$\acute{e}$. and Caesalpinia sappan Linn$\acute{e}$. may play important roles in antimicrobial activities against S. gallinarum and S. epidermidis.

Characteristics of the Conversion Pigment from Gardenia jasminoides Yellow Pigment (치자황색소로부터 변환된 색소의 특성)

  • Jeong, Hyung-Seok;Park, Keun-Hyung
    • Korean Journal of Food Science and Technology
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    • v.30 no.2
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    • pp.319-323
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    • 1998
  • Conversion of Gardenia jasminoides yellow pigment into blue-green pigment by 8 bacterial species was examed. Bioconversion pattern can be categorized into three types according to absorption spectra characteristics. The same pattern of the value of ${\Delta}E$ estimated by color differencemeter was also observed. Conversion rate by S. epidermidis was faster than other bacterial species. It took 16 hour for S. epidermidis to convert pigment at $37^{\circ}C$. Gardenia jasminoides yellow pigment and conversion pigment were completely separated by Amberlite XAD column chromatography with $H_2O-MeOH$ solvent system. Storage stability of the conversion pigment was better than Gardenia jasminoides yellow pigment.

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Synergistic Antimicrobial Effect of Patrinia scabiosaefolia and Forsythiae fructus Extracts on Food-borne Pathogens (식중독 유발 세균의 증식에 미치는 패장과 연교 추출물의 상승 효과)

  • Bae Ji-Hyun;Son Kug-Hee;Lee Eun-Joo
    • Microbiology and Biotechnology Letters
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    • v.33 no.2
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    • pp.130-135
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    • 2005
  • To investigate the antimicrobial effect of the Patrinia scabiosaefolia extracts against food-borne pathogens, we extracted the P. scabiosaefolia with methanol at room temperature and the fractionation of the methanol extracts was carried out by using petroleum ether, chloroform, ethyl acetate, and methanol, respectively. The antimicrobial activity of the P. scabiosaefolia extracts was determined by using a paper disc method against food-borne pathogens and food spoilage bacteria. The ethyl acetate extracts of P. scabiosaefolia showed the highest antimicrobial activity against Escherichia coli and Shigella sonnei. Synergistic effect in inhibition was observed when P. scabiosaefolia extract was mixed Forsythiae fructus extract as compared to each extracts alone. Finally, the growth inhibition curves were determined by using ethyl acetate extracts of P. scabiosaefolia against Staphylococcus epidermidis and Shigella sonnei. The ethyl acetate extract of P. scabiosaefolia had strong antimicrobial activity against S. sonnei at the concentration of 4,000 ppm. At this concentration, the growth of S. Sonnei was retarded more than 72 hours and up to 48 hours for S. epidermidis. These results suggest that the ethyl acetate extracts of P. scabiosaefolia can be used for the efficient material against the growth of S. epidermidis and S. sonnei.

The Early Detection of the Gram Positive Bacteria using Sonification and Fluorescent Dye in the Field (현장에서 초음파 파쇄와 형광시약을 이용한 그람 양성균의 조기 탐지)

  • Ha, Yeon-Chul;Choi, Ki-Bong;Choi, Jung-Do
    • KSBB Journal
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    • v.21 no.5
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    • pp.347-352
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    • 2006
  • This study was carried out to establish the optimum condition for cell disruption with a sonificator in the detection of the gram positive bacteria, Bacillus globigii and Streptococcus epidermidis for the purpose of developing automatic fluorometer. The efficiency of sonificator on the Bacillus globigii and Streptococcus epidermidis disruption differed greatly according to the diameter of sonificator probe tip. The larger sonificator probe diameter showed greater disruption. Bacillus globigii was more disruptive than Streptococcus epidermidis. Sonificator probe of the 13 mm diameter was the most efficient one when sample was sonificated for 20 seconds. The detection limits of Bacillus globigii and Streptococcus epidermidis were $10^5CFU/m{\ell}\;and\;5{\times}10^5CFU/m{\ell}$ respectively when samples were sonificated for 20 seconds with a sonificator probe of 13 mm diameter.

Disseminated Septic Lesions Caused by Staphylococcus epidermidis Mimicking Cysticercosis Detected on Ultrasonography in a Pediatric Patient with Acute Myeloid Leukemia (급성 골수성 백혈병 소아환자에서 초음파 검사에서 낭미충증으로 오인된 표피 포도알균에 의한 파종 감염 병변이 발생한 증례)

  • Lee, Jae Min;Choi, Joon-Sik;Yoo, Keon Hee;Kim, Yae-Jean;Kim, Sun-Ja
    • Pediatric Infection and Vaccine
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    • v.27 no.2
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    • pp.134-139
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    • 2020
  • Staphylococcus epidermidis is a part of the normal skin flora of humans. However, it can cause serious infections in people exposed to foreign bodies or in immunocompromised patients. A 13-year-old boy was hospitalized with fever and myalgia. Painful nodular lesions were detected on the scalp, arms, and legs. Pancytopenia and blasts were present in the peripheral blood. He was diagnosed with acute myeloid leukemia. Magnetic resonance imaging of the whole body showed multiple peripheral rim-enhancing, cyst-like lesions. Ultrasonography showed echogenic nodules inside the cystic lesions in the intramuscular space of the arms and legs. Therefore, cysticercosis was strongly suggested initially. However, an abscess was confirmed on sono-guided biopsy and S. epidermidis was isolated from a microbial culture of the tissue. We report a case of multiple disseminated lesions caused by S. epidermidis in a leukemia patient, initially mistaken for cysticercosis.