• Title, Summary, Keyword: S. epidermidis

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A Study on the Cosmetic Preservative Effects of Cornus officinalis seed Extracts (산수유 종자 추출물의 화장품 방부효능에 관한 연구)

  • Yang, Jae-Chan
    • Journal of the Korean Applied Science and Technology
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    • v.33 no.2
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    • pp.333-341
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    • 2016
  • To evaluate the possibility as a natural preservative of Cornus officinalis seed extracts, we investigated the anti-microbial activity and preservation effect of cosmetics. Cornus officinalis seed extracted with 1,3-Butylene Glycol(1,3-BG) and Ethanol(EtOH). As a result, Cornus officinalis seed extracts showed anti-microbial activity at Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acnes and Cornus officinalis seed 1,3-BG extracts(COS-A) showed $26.7{\pm}4.0mm$ clear zone at the $1000.0{\mu}l/ml$. Minimum inhibitory concentration of S. epidermidis was observed at Cornus officinalis seed EtOH extracts(COS-B) $25.0{\mu}l/ml$. The result of challenge test for preservation effect on cosmetics, S. aureus and S. epidermidis was 100% eliminated from emulsion containing COS-A and COS-B after 7 days. These results suggest that Cornus officinalis seed extracts may have possibility as the natural preservative instead of synthetic perservative on cosmetics.

Causative Organisms of Neonatal Sepsis (신생아 패혈증의 원인 병원체에 대한 조사)

  • Kim, Kyung-Ah;Shin, Son-Moon;Moon, Han-Ku;Park, Young-Hoon
    • Yeungnam University Journal of Medicine
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    • v.16 no.1
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    • pp.60-68
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    • 1999
  • A nationwide survey was conducted to investigate the annual occurrence rate of neonatal sepsis, maternal risk factors in neonatal sepsis, localized infection in neonates, causative organisms in nosocomial infection and the most common causative organism for neonatal sepsis in Korea. Clinical and bacteriological data wele collected from 37 neonatal units to perform retrospective review of the medical records of the newborn infants who were confirmed as having neonatal sepsis and whose blood culture was collected to isolate organisms for one year study period from January to December in 1997. 78,463 neonates were born at 37 hospital in 1997, and 20,869 neonates were admitted to the neonatal units, During this period, 772 episodes of neonatal sepsis were recorded in 517 neonates. The occurrence rate of neonatal sepsis was 0.73%(0~2.95%). Male to female ratio was 1.15:1, and 303 cases(42.1%) were born prematurely. The main pathogens of early onset of sepsis were S. aureus(20%), S. epidermidis(14.4%) and coagulase negative staphylococcus(14. 4%). Gram negative bacilli including Enterobacter spp (7.2%), E. coli(5.1%), Klepstella(4.5%), Pseudomonas(3.7%) and Enterobacter faectum(3.6%) accounted for 24.1% of sepsis. Group B beta-hemolytic streptococcus were isolated only in two cases. Common obstetric factors were PROM(21.1%), difficulty delivery(18.7%), fetal tachycardia(5.3%), chorioamnionitis(4.9%), and maternal fever(4.7%). The main pathogens of late-onset sepsis were S. aureus(22.3%), S. epidermidis(20.4%) and CONS(9.9%). There were 6 cases(1.0%) of Candida sepsis, Frequent focal infections accompanying sepsis were pneumonia(26.1%), urinary tract infection(10.5%), meningitis(8.2%), and arthritis(3.6%), S. epidermidis(22.0%) and s. aureus(21.7%) were also the most common pathogens in 373 nosocomial infection.

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Antimicrobial Activity of Hwangryunhaedok-tang Extract against Keratitis (황련해독탕 추출액의 각막염 원인균에 대한 항균효과)

  • Kim, In-Soo;Seo, Jin-Jong;Kim, Kwang-Gon;Ha, Dong-Ryong;Shin, Min-Koo;Kim, Eui-Su;Jeon, Sang-Yun
    • The Journal of Internal Korean Medicine
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    • v.35 no.3
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    • pp.288-297
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    • 2014
  • Objectives: The goal of this project was to measure the antibacterial effectiveness of Hwangryunhaedok-tang (barberry root decoction for detoxification) and its constituents. All of the active ingredients including the final product were prepared using high-pressure sterilization for use as medicinal eye drops. The varying forms of Hwangryunhaedok-tang (barberry root decoction for detoxification) were used to treat Staphylococcus epidermidis and Enterococcus faecalis which are found in keratitis. Methods: The antibacterial effect was measured by observing the presence or absence of antimicrobial activity when treated with varying concentrations of Hwangryunhaedok-tang extract. The tests were performed using a dosage of $70{\mu}l$ dosages of 100%, 50%, 10% and 1% the extracted solution by the minimum growth inhibitory concentration measurement. Antimicrobial activity was measured by examining the correlation between dosage strength and bacterial activity from $70{\mu}l$ to $10{\mu}l$ at the same concentration. Results: 1. Hwangryunhaedok-tang (barberry root decoction for detoxification), Phellodendri Cortex, and gardenia didn't show any antimicrobial effects against S. aureus, S. epidermidis, or E. faecalis. 2. Barberry root showed antimicrobial effects against S. aureus and S. epidermidis depending on the levels of concentration but didn't show any antimicrobial effects against E. faecalis. 3. Skullcap showed antimicrobial effects against S. aureus and S. epidermidis when a dosage of 100% extract $70{\mu}l$ was used. However, did not show any antimicrobial effects at all against E. faecalis. Conclusions: Hwangryunhaedok-tang (barberry root decoction for detoxification) and its constituents such as barberry root, phellodendri cortex, skullcap, and gardenia, can be used as an alternative to antibiotic medicinal eye drops to treat keratitis. However, further research on effective uses of and efficient extraction methods are needed.

Screening of Lactic Acid Bacteria as a Starter Culture in Fermented Sausage (발효소시지 제조에 적합한 스타터 선발)

  • Yoo, Seon-A;Seo, Seung-Ho;Park, Seong-Eun;Son, Hong-Seok
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.8
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    • pp.1289-1295
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    • 2014
  • The aim of this study was to select the most suitable starter cultures for production of fermented sausages. A total of 27 strains isolated from Korean fermented foods and natural substances were characterized with respect to their physicochemical properties in a fluid (submerged) model system modified according to the special conditions of fermented sausages. Three of these strains were pre-selected for testing as potential cultures based on their ability to grow fast and initiate rapid acidification. The selected strains were identified by API and partial sequence analysis of 16S rRNA. The results exhibited sequence similarity to known sequences of Staphylococcus warneri, Staphylococcus epidermidis and Lactobacillus plantarum. Among them, relatively good growth properties and nitrite reduction activities were detected for S. epidermidis and L. plantarum and low pH values and high total acidities were observed in the model system fermented with these isolates compared with reference strains.

Adsorption of $\textrm{Pb}_{2+}$ in the components of bacterial cell membrane

  • Kim, Mal-Nam
    • Journal of Microbiology
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    • v.33 no.4
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    • pp.278-282
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    • 1995
  • S. epidermidis cell was fractionated into cell wall, cell membrane and cytoplasm. The cell membrane adsorbed the most abundant $\textrm{Pb}_{2+}$ per unit dry weight of the three fractions tested. Adsorption behavior of $\textrm{Pb}_{2+}$ in lipid and protein, which are the main components of the cell membrane, indicated that phosphatidylethanolamine and phosphatidylinositol having phosphoryl group and gangliosides containing carboxyl groups adsorbed much more $\textrm{Pb}_{2+}$ than triglycerides lacking any chargeable functional groups. Protein purified from cell membrane adsorbed larger amount of $\textrm{Pb}_{2+}$ than total native cell membrane or cell membrane lipid.

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황련의 증량에 따른 전탕액이 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구

  • Seo, Hyeong-Sik
    • Journal of Pharmacopuncture
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    • v.10 no.1
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    • pp.79-84
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    • 2007
  • Objective : This experimental study was performed to investigate the effect of Coptidis rhizoma extract compared with quantity on Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by dropping to 50${\mu}l$ according to density Coptidis rhizoma extract(100%, 10%, 1%, 0.1%) compared with quantity(40g, 80g, 160g). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on Staphylococcus epidermidis in Coptidis rhizoma extract was showed anti-bacterial potency compared with quantity and density in 100% and 10% of all samples(40g, 80g, 160g). 2. MIC on Staphylococcus epidermidis in Coptidis rhizoma extract(40g, 80g, 160g) was showed anti-bacterial potency compared with quantity all samples($20{\mu}l$, $30{\mu}l$, $40{\mu}l$, $50{\mu}l$) in 100% density, but anti-bacterial potency of 80g, $50{\mu}l$ Coptidis rhizoma extract decreased compared with 40g, $50{\mu}l$. 3. MIC on Staphylococcus epidermidis in 40g Coptidis rhizoma extract was showed in $50{\mu}l$, $40{\mu}l$ of 10% density, 80g Coptidis rhizoma extract was showed in $50{\mu}l$ of 10% density, 160g Coptidis rhizoma extract was showed in $50{\mu}l$, $40{\mu}l$, $30{\mu}l$ of 10% density. Conclusions : Coptidis rhizoma extract was showed anti-bacterial potency compare with quantity and density. In herbal drug, anti-bacterial potency compare with quantity and density must be studied.

Antimicrobial Effect of Forsythiae Fructus Extracts on Several Food-Borne Pathogens (연교 추출물의 항균활성 검색 및 일부 식중독균의 증식에 미치는 영향)

  • Bae Ji-hyun;Kim Hong-youn;Jang Ji-Youn
    • Korean journal of food and cookery science
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    • v.21 no.3
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    • pp.319-325
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    • 2005
  • This study was performed to investigate the antimicrobial effect of the Forsythiae Fructus extracts against food-borne pathogens. First, Forsythiae Fructus was extracted with methanol at room temperature and the methanol extracts were fractionated by using petroleum ether, chloroform, ethyl acetate, and methanol. The antimicrobial activity of the Forsythiae Fructus extracts was determined by using a paper disc method against food-borne pathogens and food spoilage bacteria. The methanol extracts of Forsythiae Fructus showed the highest antimicrobial activity against Salmonella paratryphimurium and Salmonella typhimurium. A synergistic effect in inhibition was observed when Forsythiae Fructus extract was mixed with Ulmus davidiana Japonica extract as compared to each extract alone. Finally, the growth inhibition curves were determined by using ethyl acetate extracts of Forsythiae Fructus against Shigella flexneri and Salmonella paratyphimurium. The aqueous extract of Forsythiae Fructus had strong antimicrobial activity against Staphylococcus epidermidis at the concentration of 10,000 ppm. At this concentration, the growth of Shigella fexneri was retarded for more than 24 hours and for up to 12 hours for Staphylococcus epidermidis. In conclusion, the methanol extracts of Forsythiae Fructus efficiently inhibited Staphylococcus epidermidis and Shigella flexneri.

Antipathogenic Activity of Lactobacillus plantarum Isolated from Pickled Mulberry Leaf (뽕잎 장아찌로부터 분리된 Lactobacillus plantarum 균주의 유해균 증식 억제 활성)

  • Park, Eun-Hee;Kim, Myoung-Dong
    • Microbiology and Biotechnology Letters
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    • v.44 no.2
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    • pp.163-170
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    • 2016
  • Strains of lactic acid bacteria were isolated from a variety of fermented foods collected in Korea. The strain L2167 showed a strong antipathogenic activity against Bacillus cereus, Listeria monocytogenes, Salmonella Typhimurium, Staphylococcus aureus, and Staphylococcus epidermidis. L2167 was identified as Lactobacillus plantarum by sequence analysis of its 16S rRNA gene. Scanning electron microscopy revealed rough and wrinkled morphology of B. cereus, L. monocytogenes, S. Typhimurium, S. aureus, and S. epidermidis cell membranes after treatment with a crude cell extract of L. plantarum L2167, indicating that Lactobacillus plantarum L2167 might destroy the cell membrane of pathogenic bacteria. The optimal temperature and initial medium pH for Lactobacillus plantarum L2167 growth were 35℃ and 5.5, respectively. Lactobacillus plantarum L2167 was more sensitive to NaCl than Lactobacillus plantarum KCTC21004, used as a control strain. Lactobacillus plantarum L2167 is expected to be developed as a prominent starter strain for efficient inhibition of growth of pathogens.

Antimicrobial Susceptibility test and DNA gyrase analysis of Coagulase-negative Staphylococci isolated (Coagulase-negative Staphylococci 분리 균주의 항생제 감수성 검사 및 DNA gyrase 분석)

  • Na, Hun-Taek
    • The Korean Journal of Vision Science
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    • v.20 no.3
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    • pp.249-258
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    • 2018
  • Purpose : We investigated the susceptibility of quinolone series and vancomycin, erythromycin antibiotics for isolates of Coagulase-negative Staphylococci and analyzed the DNA gyrase gene. Methods : Antibiotic susceptibility ofloxacin, ciprofloxacin, levofloxacin, vancomycin, and erythromycin were studied in six strains of coagulase-negative Staphylococci detected in corneas of suspected bacterial keratitis. Antibiotic susceptibility ofloxacin, ciprofloxacin, levofloxacin, vancomycin, and erythromycin were studied in six strains of coagulase-negative Staphylococci detected in corneas of suspected bacterial keratitis. and the genomic DNA of each strain was extracted. After amplifying PCR, DNA of gyraseA gene was analyzed. Results : In the antibiotic susceptibility test, ofloxacin, ciprofloxacin, levofloxacin and vancomycin susceptibility, and erythromycin resistance were observed in strains of S. epidermidis (SE-2, SE-3), S. hominis (SH-1) and S. saprophyticus (SS-1), showing that all of the other strains were susceptible. In the gyraseA gene analysis, amino acid double mutation was observed from S. epidermidis (SE-1) strain as $Met75{\rightarrow}Thr$ and $Met93{\rightarrow}Val$, and many silent mutations were observed in strains of S. warneri (SW-1) and S. saprophyticus (SS-1). Conclusions : The isolates of Coagulase-negative Staphylococci showed resistance to erythromycin antibiotics, and amino acid double mutation and silent mutation were observed from gyraseA gene analysis. Therefore, although it is not resistance now, potential mutations would be thought that can cause resistance at any time.

Evaluation of Antimicrobial Activity and Mutagenicity of Ozonized Olive Oil (오존화 올리브유의 항균성 및 변이원성 평가)

  • Jang, Il-Woong;Lee, Seung-Jae;Ahn, Jeung-Youb;Miura, Toshiaki;Jung, Mun-Yhung;Choi, Dong-Seong
    • Korean Journal of Food Science and Technology
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    • v.38 no.6
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    • pp.805-809
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    • 2006
  • Ozonized olive oil was tested for its mutagenic potential in a Salmonella/microsome assay. Additionally, antimicrobial activity was tested against Propionibacterium acnes, Staphylococcus epidermidis, Staphylococcus aureus and Pseudomonas aeruginosa, pathogenic strains related to acne, using the paper disk and agar dilution method. Ozonized olive oil showed antimicrobial activities against all the strains tested, with minimal inhibitory concentrations (MICs) values in a range of 2${\sim}$10 mg/mL. Mutagenicity of ozonized olive oil was evaluated with Salmonells typhimurium TA98, TA100 and Ta1535, with and without addition of S9 mixture. No increase in the number of $his^{+}$ revertants over the negative control (solvent and non-ozonized olive oil) values was observed with TA98 (1,000 ${\mu}g/plate$), TA100 (1,500 ${\mu}g/plate$) and TA1535 (1,500 ${\mu}g/plate$) strains. The results from this study suggested that ozonized olive oil does not show any mutagenic potential.