• Title, Summary, Keyword: S. epidermidis

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Conversion Patterns of Yellow Pigment from Gardenia jasminoides by Staphylococcus epidermidas and Lactobacillus plantarum (Staphylococcus epidermidas와 Lactobacillus plantarum에 의한 치자황색소의 변환양상)

  • Jeong, Hyung-Seok;Park, Keun-Hyung
    • Korean Journal of Food Science and Technology
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    • v.31 no.5
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    • pp.1184-1187
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    • 1999
  • The Gardenia jasminoides yellow pigment and converted pigments were completely separated by Amberlite XAD-4 column chromatography. These Pigments were gel filtrated on Sephadex LH-20 column chromatography. The characteristics of absorption spectra of eluate and fractionated pigments were investigated. The pigment converted by Lactobacillus plantarum showed a single blue color with an absorption peak at 588 nm and its molecular size was bigger than that of crocetin. The pigment, converted by Staphylococcus epidermidis, Showed blue-green color, which was composed of yellow color with an absorption peak at 418 nm and blue color at 588 nm. Molecular size of the yellow pigment was smaller than crocetin and that of blue color.

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A Study of Antioxidative and Antimicrobial Effects of Coffee Residue Extracts (커피 부산물의 항산화와 항균력에 관한 연구)

  • Lee, Byeong-Eun;Yang, Jae-Chan;Kim, Bo-Ae
    • Journal of the Korean Applied Science and Technology
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    • v.33 no.3
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    • pp.606-613
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    • 2016
  • The purpose of this study is to analyze the possibility of a residual product of coffee (RC). RC oil extracted with n-hexane at $60({\pm}10)^{\circ}C$ for 24 hours. In this study, the cytotoxicity of RC oil was observed against B16F10 melanoma cells and RAW 264.7 macrophage cells by water solubletetrazolium salt-1 assay, and The RC oil measured by methods of DPPH radical scavenging and antimicrobial activities in Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Candida albicans. As a result, the RC oil treatment-related cytotoxic effects appeared on B16F10 melanoma cells from 0.125 to $2{\mu}{\ell}/m{\ell}$ and RAW 264.7 macrophage cells from 0.125 to $0.5{\mu}{\ell}/m{\ell}$ concentrations in this study. RC oil is radical scavenging activity concentrations on dependent. The antimicrobial activity of RC oil ($150{\mu}l/{\ell}$) was determined by clear zone method. Straphylococcus epidermidis, Straphylococcus aureus, Escherichia coli, Candida albicans showed clear zone by each $11.3{\pm}0.4$, $12.{\pm}0.7$, $12.0{\pm}0.0$, $0.0{\pm}0.0mm$. It is suggested that RC oil have effects on the cytotoxicity, antioxidant and antimicrobial that could be applicable to cosmetics as a new material.

A Study of the Antioxidant Activities and Whitening Activities of Areca semen Extracts as Cosmetic Ingredient (화장품 소재로서 빈랑자의 항산화 및 미백활성에 관한 효과)

  • Kang, Hee Cheol;Cha, Mi Yeon;Kim, Jae Young
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.41 no.3
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    • pp.269-277
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    • 2015
  • Herbal plant extracts are good resources to find functional compounds for cosmetic ingredient. In this study, the extract of Areca semen (A. semen) was studied for melanogenesis inhibition and antioxidant activity. The results showed that ethyl acetate fraction of A. semen contained phenolic contents, $301.35{\pm}0.88{\mu}g/mg$, and exhibited potent antioxidant activity with $IC_{50}$ value of $1.02{\pm}0.07{\mu}g/mg$. Further, FRAP value exhibited potent antioxidant activity with $9.07{\pm}0.36mM$. Disk diffusion assay was performed for antibacterial activity. Ethyl acetate fraction of A. semen showed antibacterial activity against Staphylococcus aureus (S. aureus) and Staphylococcus epidermidis (S. epidermidis) at $80{\mu}g/mL$, whereas it showed no significant antibacterial activity against Escherichia coli (E. coli). The results of cell viability indicated that ethyl acetate fraction did not show cytotoxicity to B16/F1 cells at $80{\mu}g/mL$ and showed significant cytotoxicity at $100{\mu}g/mL$ of concentration and showed inhibition of melanin synthesis inhibitory, $29.78{\pm}0.31%$ at $80{\mu}g/mL$. Furthermore, mRNA expressions of tyrosinase and MITF were decreased after treatment with ethyl acetate fraction in a dose-dependent manner. As a result, the ethyl acetate fraction of A. semen could be considered as potential as whitening agents.

Detection and Characteristics of Coagulase-Negative Staphylococcus sp. isolated from Dairy Cattle Milk (원유에서 Coagulase-Negative Staphylococci 검출율 및 특징)

  • Kim, Jong-Hui;Kim, Bu-Min;Ham, Jun-Sang;Oh, Mi-hwa
    • Journal of Dairy Science and Biotechnology
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    • v.35 no.3
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    • pp.162-168
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    • 2017
  • Mastitis is a common and serious infection of the mammary gland in dairy cattle and has a major economic impact on the production of milk and dairy products. Bacterial mastitis is caused by several pathogens and is most frequently associated with coagulase-negative staphylococci (CNS). Although CNS are typically associated with subclinical or mild mastitis, the importance of CNS has increased as these pathogens have emerged as predominant mastitis-related pathogens in many countries. CNS can cause persistent infections, resulting in increased milk somatic cell counts and thereby affecting milk quality and decreasing milk production. Globally, Staphylococcus chromogenes, S. epidermidis, and S. simulans are the predominant CNS species in dairy cattle mastitis. Antibacterial resistance of CNS varies with species, and most CNS are susceptible to vancomycin and resistant to penicillin and tetracycline. As the most frequently isolated CNS species, some strains of S. chromogenes exhibit phenotypic resistance to ampicillin, erythromycin, oxacillin, penicillin, and tetracycline. Some strains of S. epidermidis and S. haemolyticus are only susceptible to vancomycin and rifampicin. Therefore, more studies are needed to achieve the control and prevention of CNS as environmental pathogens.

ANTIMICROBIAL EFFECT OF ESSENTIAL OILS ON ORAL BACTERIA (구강 내 세균에 대한 Essential oil의 항균효과에 관한 연구)

  • Lee, Sun-Young;Kim, Jae-Gon;Baik, Byeong-Ju;Yang, Yeon-Mi;Lee, Kyung-Yeol;Lee, Yong-Hoon;Kim, Mi-A
    • THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY
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    • v.36 no.1
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    • pp.1-11
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    • 2009
  • Essential oils are mixture of volatile, lipophilic compounds originating from plants. Essential oils have potential biological effects, i.e., antibacterial, antifungal, spasmolytic and antiplasmodial activities and insect-repellent property. In this study, five essential oils, namely R, LG, FR, O, and NM, extracted from various aromatic plants were used to test their antimicrobial activity against the oral microorganisms. The effects of essential oils were investigated against eight important bacteria, Streptococcus mutans (S. mutans), Staphylococcus aureus (S. aureus), Streptococcus sanguis (S. sanguis), Streptococcus anginosus (S. anginosus), Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans), Streptococcus sobrinus (S. sobrinus), Staphylococcus epidermidis (S. epidermidis), and Escherichia coli (E. coli). Essential oils, except NM, effectively inhibited the growth of tested oral pathogenic microorganisms dose-dependently. However, the essential oils didn't show a significant inhibitory effect against E. coli and S. epidermidis. Consequently, these results represented that essential oil-mediated anti-microbial activity was prominent against the oral pathogenic bacteria. For example, minimum bactericidal concentration(MBC) of R, LG, FR oil against A. actinomycetemcomitans was very low as 0.078 mg/mL. In addition, minimum inhibitory concentration (MIC) of R, LG, FR, O oil against S. mutans was low as 0.156 mg/mL in vitro.

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Antimicrobial Effects of Chamaecyparis obtusa Essential Oil (편백정유의 항균효과)

  • 이현옥;백승화;한동민
    • Microbiology and Biotechnology Letters
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    • v.29 no.4
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    • pp.253-257
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    • 2001
  • Essential oil of Chamaecyparis obtusa showed antimicrobial on relatively broad spectrum of bacterial and fungal species. Staphylococcus epidermidis was highly sensitive to the essential oil but Streptococcus aureus and Streptococcus mutans were not. Vibro parahemolyticus, Pseudomoas aeruginosa and Pseudomoas putida showed sensitivity at the concentration higher that 400 ppm, Thegrowth of a pathogenic yeast Candida albicans was inhibited by the essential oil above 200ppm. The radialgrowth of several filamentous fungi was also inhibited The antifungal activity of the essential oil was effective on two plant pathogens Fusarium oxysporum and Altenaria mali. These results sug-gest that essential oil of Chamaecyparis obtusa has an antimicrobial activity by inhibiting bacterial and fungal species.

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약모밀(Houttuynia cordata) 뿌리에서 추출한 순차분획물의 항균활성

  • Kim, Min-Ju;Song, Jong-Ho;Gwon, Hyeok-Dong;Son, Jeong-Eun;Park, In-Ho
    • 한국생물공학회:학술대회논문집
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    • pp.855-858
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    • 2001
  • Antimicrobial activities, extraction yields and chemical compositions of the extracts from the powder of vacuum dried Houttuynia cordata root on various solvents were investigated. Antimicrobial activity was investigated by disc diffusion method against several microorganisms, nine species of Gram positive and thirteen Gram negative bacteria. Ethanol, ethylacetate and butanol extracts exhibited potent antimicrobial activities against B. megaterium C. xerosis, S. aureus, S. epidermidis and E.coli. Chloroform and aqueous extracts from H. cordata showed weak antimicrobial activity against the tested bacteria. Ethylacetate and butanol extracts exhibited strong antimicrobial activity against the tested microorganisms. The extraction yields were 15.7%, 3.7%, 0.13%, 0.5% and 5.9% in ethanol, chloroform, ethylacetate, butanol and aqueous fractions, respectively. To further elucidate the effective components, solvent extracts was analyzed by GC/MSD.

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Composition and Antimicrobial Activities of Essential Oils in the Peel of Citrus Fruits

  • Oh, Hyun-Jeong;Ahn, Hyo-Min;Kim, Sang-Suk;Yun, Pil-Yong;Jeon, Gyeong-Lyong;Ko, Young-Hwan;Riu, Key-Zung
    • Journal of Applied Biological Chemistry
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    • v.50 no.3
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    • pp.148-154
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    • 2007
  • Volatile components of the essential oils of Satsuma mandarin (C. unshiu), Dangyuza (C. grandis), Yuza (C. junos), Byungkyul (C. playtymamma), Jinkyul (C. sunki), and Hakyul (C. natsudaidai) grown in Jeju Island were isolated from the fruit peels by hydro distillation and determined by GC-MS. GC-MS analysis identified 58 compounds, with main components being d-limonene $(64.01{\sim}79.34%),\;{\beta}-myrcene\;(3.01{\sim}26.53%),\;{\gamma}-terpinene\;(0.11{\sim}12.88%),\;{\beta}-pinene\;(0.78{\sim}4.74%),\;and\;{\alpha}-pinene\;(1.01{\sim}2.55%)$. Differences in compositions and contents of the essential oils were observed among citrus varieties. Effects of citrus oils on growth inhibitions of Escherchia coli, Staphyllococcus epidermidis, and Candida albicans were investigated using disc diffusion assay and minimal inhibitory concentration (MIC) assay. The essential oils inhibited growths of the test organisms, exhibiting higher levels of activity against Gram-positive S. epidermidis (MIC values $0.04{\sim}0.17mg/mL$), whereas Gram-negative E. coli was moderately resistant (MIC values $1.66{\sim}20.30mg/mL$). MIC of citrus essential oils ranged from $0.82{\sim}23.69mg/mL$ against C. albicans. The essential oils obtained from C. sunki, C. grandis, and C. playtymamma showed the highest antimicrobial activities against S. epidermidis and C. albicans, indicating their potential as natural antimicrobial agents.

Radiation Preparation and Characterization of Antimicrobial Hydrogels (방사선 이용 항균성 천연추출물 함유 하이드로겔 제조 및 특성 연구)

  • Gwon, Hui-Jeong;Park, Eun Ji;Choi, Jong-Bae;Lim, Jong-Young;Jeong, Jin-Oh;Shin, Young Min;Jeong, Sung In;Park, Jong-Seok;Lim, Youn-Mook
    • Journal of Radiation Industry
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    • v.7 no.1
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    • pp.87-91
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    • 2013
  • Taraxacum platycarpum (TP) has been used for years without restriction on usual dose for its non-toxic nature and nonexistence of the side effects. To develop antimicrobial hydrogel, poly (vinylalcohol) (PVA) hydrogels containing the aqueous extracted TP as an antimicrobial agent were prepared by using gamma-rays irradiation. The antimicrobial activities of the TP hydrogels were tested against Staphylococcus aureus and Staphylococcus epidermidis by disc diffusion method. The inhibition zones (IZ) of the TP extracts and TP hydrogels against S. aureus were 16 mm and 20 mm and against S. epidermidis was 10 mm and 13 mm, respectively. In conclusion, the TP hydrogel that has an excellent antimicrobial activity was proved to be a valuable material for functional skin care.

Bacteriological Research for the Contamination of Equipment in Chest Radiography (영상의학과 흉부 엑스선 촬영 기기의 세균 오염도)

  • Choi, Seung Gu;Song, Woon Heung;Kweon, Dae Cheol
    • Journal of radiological science and technology
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    • v.38 no.4
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    • pp.395-401
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    • 2015
  • The purpose is to determine the degree of contamination of the equipment for infection control in chest radiography of the radiology department. We confirmed by chemical and bacterial identification of bacteria of the equipment and established a preventive maintenance plan. Chest X-ray radiography contact area on the instrument patients shoulder, hand, chin, chest lateral radiography patient contact areas with a 70% isopropyl alcohol cotton swab were compared to identify the bacteria before and after sterilization on the patient contact area in the chest radiography equipment of the department. The gram positive Staphylococcus was isolated from side shoots handle before disinfection in the chest radiography equipment. For the final identification of antibiotic tested that it was determined by performing the nobobiocin to the sensitive Staphylococcus epidermidis. Chest radiography equipment before disinfecting the handle side of Staphylococcus epidermidis bacteria were detected using a disinfectant should be to prevent hospital infections.