• Title, Summary, Keyword: SPT

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Genetic relationship between the SPT3 gene and ARS/cAMP pathway in yeast cell cycle control (Genetic Relationship between the SPT3 Gene and RAS/cAMP Pathway in Yeast Cell Cycle Control)

  • Shin, Deug-Yong;Yun, Jean-Ho
    • Journal of Microbiology
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    • v.34 no.2
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    • pp.158-165
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    • 1996
  • The signal transduction pathways through the RAS gene product and adenyl cyclease play a critical role in regulation of the cell cycle in yeast, Saccharomyces cerevisiae. We examined the genetic relationship between the spt3 gene and ras/cAMP pathway. A mutation in the SPT3 gene suppressed cell cycle arrest at the G1 phase caused by either an inactivation of the RAS or CYR1 gene which encodes a yeast homologue of human ras proto-oncogene or adenyl cyclase, respectively. The phenotypes such as sporulation and heat shock resistancy, that resulted from a partial inactivation of the RAS or CYR1 genes, were also suppressed by the spt3 mutation. Expression of the SSA1 gene encoding one of th heat shock proteins (Hsp70) can be induced by heat shock or nitrogen starvation. Expression of this gene is derepressed in cry1-2 and spt3 mutants. The bcy 1 mutation repressed by the bcy1 mutation, but not in spt3 mutants. These results suggest that the SPT gene is involved in expression of genes that are affected by the RAS/cAMP pathway.

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An Experimental Study about the Properties of Stress Wave in SPT Model Rod (SPT 모형 롯드에서 응력파의 전파 특성에 관한 실험적 연구)

  • 권기철;서원석;김범상;김동수;이병식
    • Journal of the Korean Geotechnical Society
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    • v.18 no.4
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    • pp.95-104
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    • 2002
  • The theoretical and analytical studies on dynamic signals(force and acceleration signals) measured at SPT rod are actively made. In this paper, the characteristics of stress wave(force signals) in SPT rod were studied experimentally using model rod system. It was noted that the amplitude ratios of reflected wave over incident wave in different end conditions were well matched with theoretical results. The impedance of soil could be determined if the characteristics of incident and reflected waves could be evaluated quantitatively in situ SPT test. On the other hand, the results of model tests showed that the effect of reflected wave caused by side friction was negligible, and therefore, the analysis model considering only end resistance of sampler could be applicable in dynamic analysis of SPT.

The expression of human Spt16 is associated with cell proliferation (인간 Spt16 단백질 발현과 세포 증식 사이의 연관성에 관한 연구)

  • Gwak, Jung-Sug;Cho, Mun-Ju;Ryu, Min-Jung;Oh, Sang-Taek
    • Journal of Life Science
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    • v.17 no.3
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    • pp.381-385
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    • 2007
  • Facilitates chromatin transcription (FACT) is a chromatin-specific elongation factor required for transcription of chromatin templates in vivo and in vitro. FACT consists of human homologue of the Saccharomyces cerevisiae Spt16/Cdc68 protein (hSpt16) and the high mobility group-1-like protein structure-specific recognition protein-1 (SSRP-1). Here we show that the protein level of hSpt16 is massively down-regulated in quiescent T98C cells using both immunofluorescence and western blot analysis. In contrast, we observe high level of the hspt16 expression in the proliferative T98G cells. Interestingly, the expression of SSRP-1 is not altered in both quiescent and proliferative states. Taken together, our findings implicate that the expression of hSpt16 is associated with the proliferative state and can be used as a proliferation marker.

Effect of Sopung-tang on Glutamate-Induced Apoptosis in C6 Glial Cells (소풍탕(疎風湯)이 Glutamate에 의한 C6 Glial Cell의 Apoptosis에 미치는 영향)

  • Jeong, Seung-Won;Choi, Chul-Won;Kim, Bong-Sang;Moon, Byung-Soon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.22 no.6
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    • pp.1423-1430
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    • 2008
  • The water extract of Sopung-tang(SPT) has been traditionally used for treatment of psycologic disease and brain damage in oriental medicine. However, little is known about the mechanism by which the water extract of SPT rescues cells from these disease. Therefore, this study was designed to investigate the effect of SPT on the glutamate-induced toxicity of rat C6 glial cells. SPT have protective effects in glutamate-induced toxicity, which was revealed as apoptosis characterized by chromatic condensation and fragmentation and the loss of mitochondrial membrane potential in C6 glial cells. Also, SPT have inhibited the active form of caspase-3 and PARP and significantly protected the apoptotic phenomena by glutamate toxicity in C6 glial cells. However, SPT significantly recovered the depletion of GSH and inhibited the generation of ROS by glutamate in C6 glial cells. In addition, both SPT and antioxidants such as GSH and NAC protected the glutamate-induced cytotoxicity in C6 glial cells, indicating that SPT possibly have antioxidative effect. Specially, SPT were showed transcriptional factor significantly increased the activation of NF-${\kappa}B$ using the analysis of NF-${\kappa}B$ luciferase reporter system in C6 glial cells. These NF-${\kappa}B$ activation protected cells from glutamate-induced toxicity to generate the heme oxygenase-1(HO-1). Taken together, we suggest that SPT have protective effects in glutamate-induced toxicity via a antioxidative mechanism.

표준관입시험 수행 과정에서의 문제점과 개선방향

  • 백세환
    • Proceedings of the Korean Geotechical Society Conference
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    • pp.275-280
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    • 2001
  • Although important developments have taken place since ESOPT 1974 both with respect to the test method as well as the interpretation of the results, many uncertainties still remain in the Standard Penetration Test(SPT). The main pitfall of SPT is that it has not been standardized differing from its terminology and further, the possibility of standardization is very low in practice. Therefore, lack of knowledge on the equipment and method of SPT tends to cause some errors in interpretation of the results. It Is especially important to understand this tendency in domestic design, because most foundations are designed based on SPT results only. Many researchers have made an effort to minimize the uncertainties of SPf in Korea, it is not cleary defined what the most effective method of execution and interpretation of SPT Some uncertainties which many geotechnical engineers encounter in practice are introduced to discuss about improvement of test procedure and interpretation.

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Correlational Analysis of Supine Position Time and Sleep-related Variables in Obstructive Sleep Apnea Syndrome (폐쇄성 수면무호흡 증후군에서 앙와위 자세시간과 수면관련변인 간 상관관계 분석)

  • Kim, Si Young;Park, Doo-Heum;Yu, Jaehak;Ryu, Seung-Ho;Ha, Ji-Hyeon
    • Sleep Medicine and Psychophysiology
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    • v.24 no.1
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    • pp.32-37
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    • 2017
  • Objectives: A supine sleep position increases sleep apneas compared to non-supine positions in obstructive sleep apnea syndrome (OSAS). However, supine position time (SPT) is not highly associated with apnea-hypopnea index (AHI) in OSAS. We evaluated the correlation among sleep-related variables and SPT in OSAS. Methods: A total of 365 men with OSAS were enrolled in this study. We analyzed how SPT was correlated with demographic data, sleep structure-related variables, OSAS-related variables and heart rate variability (HRV). Multiple linear regression analysis was conducted to investigate the factors that affected SPT. Results: SPT had the most significant correlation with total sleep time (TST ; r = 0.443, p < 0.001), followed by sleep efficiency (SE ; r = 0.300, p < 0.001). Snoring time (r = 0.238, p < 0.001), time at < 90% SpO2 (r = 0.188, p < 0.001), apnea-hypopnea index (AHI ; r = 0.180, p = 0.001) and oxygen desaturation index (ODI ; r = 0.149, p = 0.004) were significantly correlated with SPT. Multiple regression analysis revealed that TST (t = 7.781, p < 0.001), snoring time (t = 3.794, p < 0.001), AHI (t = 3.768, p < 0.001) and NN50 count (t = 1.993, p = 0.047) were associated with SPT. Conclusion: SPT was more highly associated with sleep structure-related parameters than OSAS-related variables. SPT was correlated with TST, SE, AHI, snoring time and NN50 count. This suggests that SPT is likely to be determined by sleep structure, HRV and the severity of OSAS.

신기술신제품-HP의 새로운 인쇄표준, 확장형 프린팅 플랫폼 SPT

  • Kim, Jeong-Sang
    • 프린팅코리아
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    • pp.104-105
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    • 2006
  • 지난해 6월, HP는 새로운 인쇄 기술의 하나로 SPT(Scalable Printing Technology)를 선정하고 앞으로 출시되는 모든 제품에 적재할 것이라고 밝혔다. SPT는 HP 확장형 프린팅 기술로 빠른 속도와 고품질 인쇄 구현을 통해 인쇄시간과 비용을 절감할 수 있는 효율적인 시스템이다. SPT기술이 활용된 제품을 이용하면 집안에서도 손쉽게 고화질의 사진을 인화할 수 있으며, 소규모 업체에서도 부담 없이 고품질의 예술작품을 프린팅 할 수 있게 된다. HP의 새로운 인쇄의 표준인 HP SPT에 대해서 알아봤다.

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Effect of Sopyung-tang Extract on Insulin Secretion and Gene Expression in RIN-m5F Cells (소평탕(消平湯)이 RIN-m5F 세포에서 인슐린 분비 및 유전자 발현에 미치는 영향)

  • Youn, Sung-Sik;Cho, Chung-Sik
    • The Journal of Internal Korean Medicine
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    • v.31 no.1
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    • pp.25-39
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    • 2010
  • Background : At high glucose levels in $\beta$-cells, cell viability and insulin secretion are decreased by glucotoxicity. Sopyung-tang(SPT) had an effect on blood glucose level decrease and antioxidant enzyme activities in streptozotocin-induced diabetic rats. Objectives : This study performed a series of experiment to verify the effects of SPT extract on the cell viability, antioxidant enzyme activities, insulin secretion and insulin mRNA expression at hyperglycemic states of RIN-m5F. Methods : After treatment at various concentrations of SPT added to the RIN-m5F cells, cell viability by MTT assay, free radical-scavenging activity, SOD activity and insulin secretion were measured. Additionally, insulin-related gene expression was measured using real-time RT-PCR. Results : Compared to the control group, SPT extract showed considerable effects on RIN-m5F cell viability, DPPH radical-scavenging activity, superoxide dismutase (SOD) activity, insulin secretion and insulin-related gene expression. Conclusions : This study showed that SPT extract has an effect on $\beta$-cell cell viability, insulin secretion and insulin-related gene expression. Thus, SPT extract may be used for treatment of diabetes and its complications. Further mechanism studies of SPT seem to be necessary on the glucotoxicity and oxidative stress.

Assessment of the Usefulness of an IMRT Plan Using a Shell-Type Pseudo Target with Patients in Stage III or IV of NSCLC (비소세포폐암 III, IV기 환자에 있어서 Shell-Type Pseudo Target을 이용한 세기 조절 방사선치료계획기법의 유용성 평가)

  • Lee, Sang-Bong;Park, Ki-Ju;Park, Du-Chan;Kim, Man-Wo;Kim, Jun-Gon;Noh, Sung-Hwan
    • The Journal of Korean Society for Radiation Therapy
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    • v.24 no.2
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    • pp.95-106
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    • 2012
  • Purpose: The objective of this study was to investigate the usefulness of an IMRT treatment plan according to whether there was a shell-type pseudo target during radiation therapy for patients in Stage III or IV of non-small cell lung cancer (NSCLC). Materials and Methods: After setting an IMRT (Intensity-Modulated Radiation Therapy, IMRT) plan for when there was a shell-type pseudo target (SPT) and when there was none (WSPT) with 22 patients in Stage III or IV of NSCLC, the investigator analyzed dose-volume histograms (DVHs) and made assessment with dosimetric comparisons such as homogeneity index (HI) inside the tumor target, conformity index (CI) of the tumor target, spinal cord maximum dose, Esophagus $V_{50%}$, mean lung dose (MLD), and $V_{40%}$, $V_{30%}$, $V_{20%}$, $V_{10%}$, $V_{5%}$. Results: The mean CI of WSPT and SPT was $1.22{\pm}0.04$ and $1.16{\pm}0.032$ ($.000^*$), respectively, and the mean HI of WSPT and SPT was $1.06{\pm}0.015$ and $1.07{\pm}0.014$ ($.000^*$), respectively. In SPT, the mean of each CI difference decreased by $-5.16{\pm}2.54%$, while HI increased by average $0.81{\pm}0.47%$. Esophagus $V_{50%}$ recorded $14.54{\pm}12.01%$ (WSPT) and $12.14{\pm}11.09%$ ($.000^*$, SPT) with the mean of SPT differences dropping by $-26.37{\pm}25.05%$. Mean spinal cord maximum dose was $3,898.44{\pm}1,075.0$ cGy (WSPT) and $3,810.8{\pm}1,134.9$ cGy ($.004^*$, SPT) with SPT dropping by average $-3.36{\pm}5.81%$. As for lung $V_{X%}$, the mean of $V_{5%}$ and $V_{10%}$ differences was $-1.62{\pm}2.29%$ ($.006^*$) and $-1.98{\pm}5.02%$ ($.005^*$), respectively with SPT making a decrease. The mean of V20%, V30%, and V40% differences was $-3.51{\pm}3.07%$ ($.000^*$), $-4.84{\pm}6.01%$ ($.000^*$), and $-6.16{\pm}8.46%$ ($.001^*$), respectively, with SPT making a decrease with statistical significance. In MLD assessment, SPT also dropped by average $-2.83{\pm}2.41%$ ($.000^*$). Those results show that SPT allows for mean 169 cGy (Max: 547 cGy, Min: 6.4 cGy) prescription dose. Conclusion: An IMRT treatment plan with SPT during radiation therapy for patients in Stage III or IV of NSCLC will help to reduce the risk of lung toxicity and radiation-induced pneumonia by cutting down radiation doses entering the normal lung, reduce the local control failure rate during radiation therapy due to increasing prescription doses to a certain degree, and increase treatment effects.

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A study on performance analysis of synchronization clock with various clock states in NG-SDH networks (NG-SDH 망에서 다양한 클럭상태 하에서의 동기클럭 성능분석에 관한 연구)

  • Lee Chang-Ki
    • The KIPS Transactions:PartC
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    • v.13C no.3
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    • pp.303-310
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    • 2006
  • This paper is to execute a study for characteristic analysis of synchronization clock and maximum network node number with various clock states, normal, SPT, LPT, in NG-SDH networks. Through the simulations, maximum network node numbers showed from 42 to 38 nodes in normal state. In SPT state, maximum network node numbers, when the last NE network applied to only SPT state, presented from 19 to 4 nodes, much less than normal state. Node numbers to meet specification in case of occurrence of SPT state in all NE networks decreased greatly. In LPT state, all maximum node numbers, when the last NE network applied to only LPT state, presented more than 50 nodes, and the results in case of occurrence of LPT state in all NE networks were also identified. However, node numbers to meet specification in case of LPT state in all DOTS networks were few large with difference between LPT and normal or SPT state.