• Title, Summary, Keyword: Saccharomyces cerevisiae

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Study on Mixed Cultures of Lactobacillus acidophilus and Saccharomyces cerevisiae in Soymilk (대두유에서의 Lactobacillus acidophilus와 Saccharomyces cerevisiae의 혼합배양에 관한 연구)

  • 유주현;오두환;공인수;박영서;임홍철
    • Microbiology and Biotechnology Letters
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    • v.16 no.2
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    • pp.131-135
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    • 1988
  • Lactobacillus acidophilus KFCC12731 and Saccharomyces cerevisiae KFCC32017 were incubated together in soymilk and the conditions for acid production were investigated. The acid production of Lactobacillus acidophilus was much higher when this organism was incubated with Saccharomyces cerevisiae in soymilk than when it was incubated alone. Optimum acid production by the mixed cultures of Lactobacillus acidophilus and Saccharomyces cerevisiae was achieved with the following conditions; a temperature of 34$^{\circ}C$, a 3:7-8:2 (OD 660) ratio of Lactobacillus acidophilus to Saccharomyces cerevisiae at inoculum, a 1.5% level of sucrose fortification or a 2.0-3.0 % level of skim milk powder fortification and a culture time of 12 hours or more.

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Physiological Characteristics and Ethanol Fermentation of Thermotolerant Yeast Saccharomyces cerevisiae OE-16 from Traditional Meju (메주에서 분리한 고온성 효모 Sccharomyces cerevisiae OE-16 의 생리적 특성과 알코올발효)

  • 김나미
    • The Korean Journal of Food And Nutrition
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    • v.12 no.5
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    • pp.490-495
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    • 1999
  • A thermotolerant yeast Saccharomyces cerevisiae OE-16 was isolated from traditional Meju was investigated on their physiological characteristics and ethanol fermentation ability. Saccharomyces cerevisiae OE-16 were able to grow up to 45$^{\circ}C$ and 40% of glucose. Saccharomyces cerevisiae OE-16 was also resistant to 15% of KCl 1,200ppm of Pb2+, Hg2+ and 500ppm of potassium sorbate. From 20% glucose media Saccharomyces cerevisiae OE-16 produced 83.4g per liter of ethanol at 3$0^{\circ}C$ and 9.5g per liter of ethanol at 4$0^{\circ}C$ for 72 hours.

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Breeding of Yeast Strain with Starch Utilizing and Alcohol Fermenting Ability by Protoplast Fusion (전분분해활성과 알코올 발효능을 보유한 효모의 육종)

  • Ju, Min-No;Hong, Sung-Wook;Kim, Kwan-Tae;Yum, Sung-Kwan;Kim, Gye-Won;Chung, Kun-Sub
    • Microbiology and Biotechnology Letters
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    • v.36 no.2
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    • pp.158-164
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    • 2008
  • The fusants which contain starch utilizing ability and alcohol fermenting ability were developed by protoplast fusion of Saccharomyces cerevisiae KOY-1 and Saccharomyces diastaticus KCTC 1804. Sacharomyces cerevisiae KH-12 was obtained by haploid induction from Saccharomyces cerevisiae KOY-1. The auxotropic mutants of yeast were obtained by using an ethylmethane sulfonate (EMS). The frequency of protoplast formation in Saccharomyces cerevisiae KOY-1 $(Met^-)$ and Saccharomyces diastaticus KCTC 1804 $(Trp^-)$ were 90.5% and 97.7%, respectively. The frequency of fusant formation was $1.79{\times}10^{-4 }$ for the regenerated protoplast and the 1,000 fusants were obtained. Fusant FA 776 was selected as a potential yeast which contain an alcohol fermenting ability in the starch medium. The genetic stability was 4.64% for 10 passages of generation. Fusant FA 776 produced 13mg/ml of alcohol in 24% starch medium and showed 1.86-fold higher alcohol fermenting ability than Saccharomyces diastaticus KCTC 1804.

Change in Growth of alcohol Fermentation Yeast with Addition of Deep Seawater (해양 심층수 첨가에 따른 알콜발효 효모의 증식 변화)

  • 김미림;정지숙;이기동
    • Korean Journal of Food Preservation
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    • v.10 no.3
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    • pp.417-420
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    • 2003
  • In order to study optimum culture condition of yeast medium added deep seawater, we examed samples with 9 yeast strains. The growth rate were measured for Saccharomyces cerevisiae 10, 11, 12, 901 and RCY and Saccharomyces kluyvery DJ97, Saccharomyces cerevisiae YJK, JK99, CMY-28 etc.. The growth of S. cerevisiae 12 was found most active in the deep seawater(hardness 500). The growth rate of S. cerevisiae 901 on medium containing deep seawater(hardness 1000) was faster than that of the yeast on medium without deep seawater. The use of deep seawater on the growth of Sacch.cerevisiae kluyvery DJ97 revealed maximum growth under the condition of hardness 200 of deep seawater and 10% of sugar concentration.

Leavening Ability of the Isolate Saccharomyces cerevisiae MF10003 in Bakery Dough (분리 효모 Saccharomyces cerevisiae MF10003의 빵반죽 발효 팽창력)

  • Oh, Jung-Suk;Min, Eung-Ki;Ahn, Chang-Hyun;Han, Yeong-Hwan
    • Journal of Life Science
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    • v.23 no.2
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    • pp.222-227
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    • 2013
  • An effective leavening yeast was isolated from raisin broth. The isolate was identified as Saccharomyces cerevisiae by comparing the homology of 18S rDNA ITS sequences and named as S. cerevisiae MF10003. S. cerevisiae MF10003 showed a 1.9-fold and 3.1-fold increase in $CO_2$ production and leavening ability, respectively, compared with the wild yeast S. ellipsoideus KCTC7243, and the dough had a rich and volatile flavor. When glucose, sucrose, fructose, and maltose were added to the culture broth as a carbon and energy source, $CO_2$ was produced in 4 hr.

The effect of the ammonium thiocyanate on the fermentation of the yeasts.(Saccharomyces brenerei-Hefe-Rasse XII, Saccharomyces formosensis No. 396 IAM) and Saccharomyces cerevisiae IAM) (Ammonium thiocyanate 농도가 주정효모(Saccharomyces brenerei Hefe-Rasse XII, Saccharomyces formosensis No. 396 IAM) 및 맥주효모(Saccharomyces cerevisiae IAM)의 발효작용에 미치는 영향)

  • 조운복;이상태
    • Korean Journal of Microbiology
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    • v.6 no.1
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    • pp.29-35
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    • 1968
  • There are many reports on the effect of salts to the fermentation of the yeasts, but there are a few reports on the effect of the ammonium thiocyanate(NH$_4CNS$) on the fermentation of the yeasts(Saccharomyces brenerei-Hefe-Rasse X11, Saccharomyces formosensis No. 396 IAM and Saccharomyces cerevisiae IAM). A study has been made on the effects of the ammonium thiocyanate on the fermentation of the above yeasts. The results of the research are as belows: 1) The addition of 0. 00001 mol. of ammonium thiocyanate makes the fermentation of the yeast(Sacch. formosensis No. 39 IAM) facilitate, but has not influenced the another yeast (Sacch. brenerei-Hefe-Rasse XII). 2) The addition of 0. 001 mol. of ammonium thiocyanate makes fermentation of Rases XII the fastest, but the on other yeast (Sacch. formosensis No - 396 IAM) was accelerated by the addition of 0. 1 mol., and it seems to have abnormal fermentation by the addition of 0. 0001 mol. The addition of ammonium thiocyanate(0. 00001-0. 001 mol.) inhibited the fermentatirn of the yeast(Sacch. cerevisiae IAM), but the concentration of 0. 1 mol. does not interrupt the fermentation of Sacch. cerevisiae IAM and increased the fermentation. 3) The order of effects to the fermentation of each yeast is Sacch. brenerei-Hefe-Rasse XII, Sacch. formosensis No. 396 IAM, Sacch. cerevisiae IAM.

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Susceptibility of Saccharomyces cerevisiae D-71 and Zygosaccharomyces rouxii SR-S to Zymolyase-20T (Zymolyase-20T에 대한 Saccharomyces cerevisiae D-71과 Zygosaccharomyces rouxii SR-S의 감수성)

  • 정창기;김찬조;이종수
    • Microbiology and Biotechnology Letters
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    • v.16 no.2
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    • pp.136-141
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    • 1988
  • Susceptibility of a thermophilic strain (D-71) of Saccharomyces cerevisiae and an osmotolerant strain (SR-S) of Zygosaccharomyces rouxii to Zymolyase-20T were studied in various renditions. Content of glucan and mannan in cell wall of Saocharomyces cerevisiae D-71 were 14.5% and 14.8%, and Zygosaccharomyces rouxii SR-S were 24.0% and 19.0%, respectively. Susceptibility of Saccharomyces cerevisiae D-71 cultured in Wickerham synthetic medium containing 0.5% of methionine and 0.1% of glucose to Zymolyase-20T was 66%, and $K_2$HPO$_4$ and aminobenzoic acid were greatly effective to susceptibility. Susceptibility of Zygosaccharomyces rouxii SR-S cultured in Wickerhnin synthetic medium containing 0.5% of peptone, 0.15% of methionine and 0.l% of glucose to Zymolyase-20T was 80%, and KI and pyridoxine were greatly effective to susceptibility. Susceptibility of Saccharomyces cerevisiae D-71 stationary cultured in YMPG medium at $25^{\circ}C$ for 12 hours was 16o1e and Zygosaccharomyces rouxii SR-S stationary cultured in YMPG medium at $25^{\circ}C$ for 30 hours was 82%.

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Functional Expression of the Neurospora crassa coq-4 Gene in Saccharomyces cerevisiae. (Saccharomyces cerevisiae에서 Neurospora crassa coq-4 유전자의 기능적 발현)

  • 김은정;최상기;천재우;오계헌;이병욱
    • Korean Journal of Microbiology
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    • v.40 no.2
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    • pp.73-80
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    • 2004
  • Coenzyme Q is a quinone derivative that acts as a lipid electron carrier in the respiratory chain located at mito-chondrial inner membrane in eucaryotes or plasma membrane in procaryotes and also functions as antioxidant. A putative Neurospora crassa coq-4 gene was cloned and functionally expressed in Saccharomyces cerevisiae coq4 mutant. Complemented S. cerevisaie mutant strain was able to produce coenzyme $Q_{6}$ and showed a normal growth rate. They also showed less sensitivities to polyunsaturated fatty acids such as linoleic acid or linolenic acid. The predicted sequence of N. crassa COQ4 is consisted of 347 amino acids with a molecular mass of 39.7 kDa and showed 35% identity and 52% similarity with that of S. cerevisiae.

Isolation and Identification of the High-Glutathione Producing Saccharomyces cerevisiae FF-8 from Korean Traditional Rice Wine and Optimal Producing Conditions (전통 발효주로부터 glutathione 고함유 효모 Saccharomyces cerevisiae FF-8의 분리.동정 및 최적 생산조건)

  • Park, Jin-Chul;Ok, Min;Cha, Jae-Young;Cho, Young-Su
    • Applied Biological Chemistry
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    • v.46 no.4
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    • pp.348-352
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    • 2003
  • In this study, strain of high-producing intracellular glutathione was isolated from Korean traditional rice wine. The isolated strain was identified as Saccharomyces cerevisiae based on the morphological, physiological and biochemical characteristics, and was designated as FF-8. The optimal condition for glutathione production by Saccharomyces cerevisiae FF-8 was obtained after cultivation with shaking for 72 hours in the YM medium. The optimal temperature, shaking rate and initial pH for the glutathione production were $30^{\circ}C$, 100 rpm and pH 6.0, respectively. The dry cell weight and glutathione concentration produced by Saccharomyces cerevisiae FF-8 were 5.2 g/l and 72.0 mg/l, respectively, under the optimal culture condition.

Production of Tyrosinase Inhibitor from Saccharomyces cerevisiae (Saccharomyces cerevisiae 로부터 Tyrosinase Inhibitor 의 생산)

  • Jang, In-Taek;Kim, Young-Hun;Kang, Min-Gu;Yi, Sung-Hun;Lim, Sung-Il;Lee, Jong-Soo
    • The Korean Journal of Mycology
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    • v.40 no.1
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    • pp.60-64
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    • 2012
  • Physiological functionalities of culture concentrates from various fungi were investigated. The culture concentrates from Saccharomyces cerevisiae Y277-3 showed the highest tyrosinase inhibitory activity of 42.7%. Among mold physiological functionalities, the culture concentrates from Aspergillus orygae CN20-3-1-4 showed the highest antioxidant activity of 15.8%. The other functionalities of fungi were very low or not detected. The intracellular tyrosinase inhibitor from Saccharomyces cerevisiae Y277-3, which showed the highest physiological functionalities was maximally produced when the strain was cultured in PD broth at $30^{\circ}C$ for 24 h.