• Title, Summary, Keyword: Salmonella

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Establishment of a live vaccine strain against fowl typhoid and paratyphoid

  • Cho, Sun-Hee;Ahn, Young-Jin;Kim, Tae-Eun;Kim, Sun-Joong;Huh, Won;Moon, Young-Sik;Lee, Byung-Hyung;Kim, Jae-Hong;Kwon, Hyuk Joon
    • Korean Journal of Veterinary Research
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    • v.55 no.4
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    • pp.241-246
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    • 2015
  • To develop a live vaccine strain against fowl typhoid and paratyphoid caused by Salmonella serovar Gallinarum biovar Gallinarum (Salmonella Gallinarum) and Salmonella serovar Enteritidis (Salmonella Enteritidis), respectively, several nalidixic acid resistant mutants were selected from lipopolysaccharide (LPS) rough strains of Salmonella Gallinarum that escaped from fatal infection of a LPS-binding lytic bacteriophage. A non-virulent and immunogenic vaccine strain of Salmonella Gallinarum, SR2-N6, was established through in vivo pathogenicity and protection efficacy tests. SR2-N6 was highly protective against Salmonella Gallinarum and Salmonella Enteritidis and safer than Salmonella Gallinarum vaccine strain SG 9R in the condition of protein-energy malnutrition. Thus, SR2-N6 may be a safe and efficacious vaccine strain to prevent both fowl typhoid and paratyphoid.

A report on the Salmonella cultures isolated in Korrea(1974) (1974년(年) 한국(韓國)에서 분리(分離)된 쌀모넬라 균(菌)에 관(關)한 보고(報告))

  • Kim, Yong-Ja;Chung, Kyung-Suck;Chun, Nam-Ho;Ryu, Young-Hat
    • The Journal of the Korean Society for Microbiology
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    • v.10 no.1
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    • pp.9-12
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    • 1975
  • The authors identified 717 Salmonella cultures among 1504 suspectable cultures collected from all over the country in 1974. According to the results obtained from the physical and biochemical tests, and the antigenic formula analysis, seven cultures of S. paratyphi A, one culture of S. paratyphi B, Six cultures of S. typhimurium, one culture of S. thompson, four cultures of S. enteritidis and six hundred and ninty eight cultures of S. typhi were confirmed. The results of antibiotics-sensitivity test on Salmonella cultures revealed that chloramphenicol, gentamycin and ampicilin were considered to be the drugs of choice according to the results from the In Vitro test performed.

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Development of Reverse Transcriptase-Polymerase Chain Reaction of fimA Gene to Detect Viable Salmonella in Milk (우유 내 활력있는 Salmonella를 검출하기 위한 fimA 유전자의 역전사중합효소 연쇄반응의 개발)

  • Choi, S.H.;Lee, S.B.
    • Journal of Animal Science and Technology
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    • v.46 no.5
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    • pp.841-848
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    • 2004
  • Rapid detection of viable Salmonella in pasteurized milk is important to protect public health from food poisoning. Reverse transcriptase-polymerase chain reaction(RT-PCR) is recognized as a molecular genetical method to differentiate between live and dead bacteria The RT-PCR in this study was designed to detect specifically viable Salmonella in milk by using the primers whose nucleotide sequences were determined based on fimA gene which encodes the submit of type 1 fimbriae. Treatment of RNA preparation with RNase-free DNase was adequate enough to destroy DNA, which may otherwise be amplified in the RT PCR Seven strains of Salmonella were detected in the RT-PCR but Escherichia coli, Shigella sonnei, Citrobacter freundii, and Klebsiella pneumoniae were not. $10^7/ml$ and $10^6/ml$ of dead Salmonella which were heat-treated in milk were detectable by using the RT-PCR but $10^5{\sim}10/ml$ of the dead bacteria were not. The sensitivity of the RT-PCR in detecting viable Salmonella was 100 cells/ml.

Characterization of Bacteriophages against Salmonella Gallinarum (Salmonella Gallinarum 박테리오파지의 특성)

  • Kim, Minjeong;Kwon, Hyuk-Moo;Sung, Haan-Woo
    • Korean Journal of Poultry Science
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    • v.44 no.3
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    • pp.181-188
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    • 2017
  • Bacteriophages are viruses that exclusively infect bacterial cells, and lytic bacteriophages can be used as a safe alternative to antibiotics for the prevention and treatment of animal diseases. In this study, we attempted to isolate and characterize bacteriophages for Salmonella enterica serovar Gallinarum (Salmonella Gallinarum), the causative agent of fowl typhoid in chickens. Ten bacteriophages were isolated from samples of sewage from seven poultry slaughterhouses. One of these isolate, designated as $SG{\Phi}-YS$ SP and classified in the family Myoviridae, produced plaques with seven Salmonella Gallinarum strains. However, no plaques were produced with any of the Salmonella enterica serovar Enteritidis strains tested, suggesting that this bacteriophage is Salmonella Gallinarum specific. To assess the lytic ability of $SG{\Phi}-YS$ SP against Salmonella Gallinarum, bacterial growth rates following inoculation of the bacteriophage were compared with the control. The $SG{\Phi}-YS$ SP treatment, with a multiplicity of infection of 10, reduced the growth of Salmonella Gallinarum by 2.21 log cfu/mL at 6 h, and 2.13 log cfu/mL at 9 h, suggesting that this bacteriophage isolate could be used for the prevention or treatment of Salmonella Gallinarum infection in chickens.

Understanding Comprehensive Transcriptional Response of Salmonella enterica spp. in Contact with Cabbage and Napa Cabbage

  • Lee, Hojun;Kim, Seul I;Park, Sojung;Nam, Eunwoo;Yoon, Hyunjin
    • Journal of Microbiology and Biotechnology
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    • v.28 no.11
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    • pp.1896-1907
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    • 2018
  • Salmonellosis is commonly associated with meat and poultry products, but an increasing number of Salmonella outbreaks have been attributed to contaminated vegetables and fruits. Enteric pathogens including Salmonella enterica spp. can colonize diverse produce and persist for a long time. Considering that fresh vegetables and fruits are usually consumed raw without heat treatments, Salmonella contamination may subsequently lead to serious human infections. In order to understand the underlying mechanism of Salmonella adaptation to produce, we investigated the transcriptomics of Salmonella in contact with green vegetables, namely cabbage and napa cabbage. Interestingly, Salmonella pathogenicity island (SPI)-1 genes, which are required for Salmonella invasion into host cells, were up-regulated upon contact with vegetables, suggesting that SPI-1 may be implicated in Salmonella colonization of plant tissues as well as animal tissues. Furthermore, Salmonella transcriptomic profiling revealed several genetic loci that showed significant changes in their expression in response to vegetables and were associated with bacterial adaptation to unfavorable niches, including STM14_0818 and STM14_0817 (speF/potE), STM14_0880 (nadA), STM14_1894 to STM14_1892 (fdnGHI), STM14_2006 (ogt), STM14_2269, and STM14_2513 to STM14_2523 (cbi operon). Here, we show that nadA was required for bacterial growth under nutrient-restricted conditions, while the other genes were required for bacterial invasion into host cells. The transcriptomes of Salmonella in contact with cabbage and napa cabbage provided insights into the comprehensive bacterial transcriptional response to produce and also suggested diverse virulence determinants relevant to Salmonella survival and adaptation.

Studies on the Sensitivity Patterns of Various Antibiotics Against Salmonella and Shigella (Salmonella 및 Shigella의 약제내성의 양상에 관한 조사연구)

  • 류재근
    • Journal of Environmental Health Sciences
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    • v.1 no.1
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    • pp.46-51
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    • 1974
  • The agar plate titrations of antibiotic sensitivities of Salmonella and Shigella isolated from human during 1967 to 1972 were studies. 1. The most effective antibiotics against Salmonella and Shigella were chloramphenicol, tetracycline, kanamycin, minomycin, and gentamycin. 2. All strain of Salmonella typhi were resistant to cloxacilline. 3. The most effective antibiotics against Shigella were kanamycin, gentamycin and minomycin.

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Analysis of Salmonella Contaminated Beef Odor Using an Electronic Nose

  • Kim, Gi-Young;Lee, Kang-Jin;Son, Jae-Yong;Kim, Hak-Jin
    • Food Science of Animal Resources
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    • v.30 no.2
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    • pp.185-189
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    • 2010
  • An electronic nose was used to identify Salmonella contamination on beef based on odors. To detect pathogen contamination of beef, $100{\mu}L$ of $10^5CFU/g$ Salmonella Enteritidis or Salmonella Typhimurium cell suspensions were spiked onto 5 g beef sirloin samples in individual vials. Odor changes over time were then measured and analyzed using an electronic nose system to identify pathogen contamination. In principle, the electronic nose system based on a surface acoustic wave (SAW) detector produced different frequency responses depending on the time and amount of each chemical. Multivariate analysis of the odor data was conducted to detect Salmonella contamination of beef. Salmonella odors were successfully distinguished from uncontaminated beef odors by principal component analysis (PCA). The PCA results showed that Salmonella contamination of beef could be detected after 4 h of incubation. The numbers of cells enumerated by standard plate count after 4 h of inoculation were $2{\times}10^6CFU/g$ for both Salmonella Enteritidis and Salmonella Typhimurium.

Development of Liposome Immunoassay for Salmonella spp. using Immunomagnetic Separation and Immunoliposome

  • Shin, Jung-Hee;Kim, Myung-Hee
    • Journal of Microbiology and Biotechnology
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    • v.18 no.10
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    • pp.1689-1694
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    • 2008
  • The ability to detect Salmonella spp. is essential in the prevention of foodborne illness. This study examined a Salmonella spp. detection method involving the application of immunomagnetic separation and immunoliposomes (IMS/IL) encapsulating sulforhodamine B (SRB), a fluorescent dye. A quantitative assay was conducted by measuring the fluorescence intensity of SRB that was produced from an immunomagnetic bead-Salmonella spp.-immunoliposome complex. The results indicated detection limits of $2.7{\times}10^{5}$ and $5.2{\times}10^{3}$ CFU/ml for Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) and Salmonella enterka subsp. enterka serovar Typhimurium (S. Typhimurium), respectivley. The signal/noise ratio was improved by using 4% skim milk as a wash solution rather than 2% BSA. In addition, higher fluorescence intensity was obtained by increasing the liposome size. Compared with the conventional plating method, which takes 3-4 days for the isolation and identification of Salmonella spp., the total assay time of to h only including 6 h of culture enrichment was necessary for the Salmonella detection by IMS/IL. These results indicate that the IMS/ IL has great potential as an alternative rapid method for Salmonella detection.

Phenotypic and Genotypic Characterization of Salmonella spp. Isolated from Pigs and their Farm Environment in Korea

  • Lim, Suk-Kyung;Byun, Jung-Ryul;Nam, Hyang-Mi;Lee, Hee-Soo;Jung, Suk-Chan
    • Journal of Microbiology and Biotechnology
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    • v.21 no.1
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    • pp.50-54
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    • 2011
  • This study's objective was to determine the prevalence of Salmonella spp. in pigs and their farm environments in Korea, and to investigate the relationship between the strains based on their phenotypic and genotypic characteristics. A total of 36 Salmonella spp. were isolated in this study: 18 isolates from 492 pigs (3.7%) and 18 isolates from 418 (4.3%) farmhouse environmental samples from 16 different pig farms. Of the Salmonella strains isolated from the numerous environmental samples, the highest prevalence was observed in slurry or manure, followed by partitions, farmer's hands, floors, water/nipples, ventilation sources, and feed, respectively. All the Salmonella isolates originating from different farms were genetically distinct. In three farms, however, identical phage types and pulse-field gel electrophoresis patterns were observed among Salmonella isolates from pig feces and environmental samples. This study suggests that environments contaminated with Salmonella could pose an infection risk to pigs on pig farms.

Characteristics of Salmonella Species Isolates from Poultry Slaughterhouses and Pork Meat Processing Plants (도계장과 돈육가공장에서 분리된 살모넬라 속의 특성 연구)

  • 이경환;권혁무;홍종해;박석기
    • Journal of Food Hygiene and Safety
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    • v.14 no.1
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    • pp.97-103
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    • 1999
  • This study was carried out to isolate and identify Salmonella species from poultry slaughterhouses and pork meat processing plants during the period from January 1997 to August 1998, and analyze resistance of antimicrobial agents and plasmid profiles of isolated Salmonella strains. A total of 15 Salmonella strains was isolated from poultry carcasses, swine carcasses, pork meats and cutting boards. Identified Salmonella strains were S. typhimurium, S. heidelberg, S. hilingdon, S. mbandaka, and S. virginia. Ten (66.7%) of 15 Salmonella strains showed resistance to antimicrobial agents and five strains (33.3%) of them were resistant to two or more antimirobial agents. Plasmids were isolated from three Salmonella isolates which had two or more plasmids.

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