• Title, Summary, Keyword: Salmonella spp

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Analysis of antimicrobial resistance and PFGE patterns of Salmonella spp. isolated from chickens at slaughterhouse in Incheon area (인천지역 닭 도축장에서 분리된 Salmonella spp.의 항생제 내성 및 PFGE 패턴분석)

  • Yang, Ha-Young;Lee, Sung-Mo;Park, Eun-Jeong;Kim, Jung-Hee;Lee, Jung-Goo
    • Korean Journal of Veterinary Service
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    • v.32 no.4
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    • pp.325-334
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    • 2009
  • Salmonella spp. are the important pathogens both economically and clinically in animals as well as human. Some of them have highly zoonotic potentials even though they are asymptomatic in animals. Therefore, the prevalence of Salmonella spp. in animals is highly concerned for human health. The present study was carried out to investigate the prevalence, antimicrobial resistance and PFGE patterns of Salmonella spp. isolated from chickens at slaughterhouse in Incheon area. The overall isolation rate of Salmonella spp. from cloaca and cecum specimens was 7.3 % (37/510). Thirty seven isolates of Salmonella spp. were identified to 5 serotypes; S. Enteritidis, S. Newport, S. Typhimurium, S. Gallinarum, and S. Derby with prevalence of 46.0%, 40.5%, 8.1%, 2.7%, and 2.7%, respectively. Resistance to nalidixic acid was found in 97.3% of Salmonella spp. isolated, followed by streptomycin (16.2%), tetracycline (16.2%), ampicillin (5.4%). Only 6 isolates (16.2%) showed resistance to more than two antimicrobials. In PFGE analysis of chicken and human isolates with Xba I, S. Enteritidis isolates from chicken showed very high similarity over 82.8% and also the similarity was very high in the comparison with human isolates. However, the higher similarity (100%) was observed among chicken isolates of S. Typhimurium. These results suggest the close genetic relatedness of Salmonella spp. isolated from chickens with human.

Epidemiological characteristics of Salmonella spp. isolated from different stages of commercial swine farms

  • Suh, Dong Kyun;Jung, Suk Chan
    • Korean Journal of Veterinary Research
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    • v.45 no.2
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    • pp.179-183
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    • 2005
  • Epidemiological characteristics of a total of 48 swine herd with diarrhea or a history of diarrhea in Gyeongsang-do between 1999 and 2000 were performed to evaluate the prevalence of Salmonella spp., their serotypes and antibiotic resistance patterns with respect to the different stages of swine production system. A total of 139 Salmonella spp. (21%) were isolated from 662 fecal samples and the overall herd prevalence of Salmonella spp. ranged from 12.5% to 88%. The average prevalence of Salmonella spp. from swine stages of suckling/nursery, grow/finisher and sow stage were 25.7%, 19.2% and 18.4%, respectively. Ten serotypes of Salmonella spp. were identified with a predominance of S. Typhimurium, S. Derby and S. Agona. Twenty-five isolates (18%) were found to be untypable. One hundred and two Salmonella isolates (73.4%) resistant to more than 1 antibiotic were characterized by 24 diverse resistance patterns, and their frequency of antibiotic resistance was highest in grow/finisher stage (83.3%). Resistance to tetracycline (TE; 67.6%), sulfamethoxazole (SU; 46.8%) and streptomycin (ST; 28%) was most common and the most common resistance patterns were TE SU (31.4%), TE (21.6%) and TE SU ST (20.6%) in order.

Rapid Detection of Salmonella spp. by Antibody Immobilization with Gold-protein A Complex (Gold-protein A Complex 항체 고정화법을 이용한 Salmonella spp.의 신속 검출)

  • Park, In-Seon;Kim, Nam-Soo
    • Korean Journal of Food Science and Technology
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    • v.31 no.1
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    • pp.1-6
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    • 1999
  • A piezoelectric (PZ) biosensor system detecting Salmonella spp. was developed. The system consisted of an oscillator, a frequency counter and an antibody-immobilized quartz crystal. An anti-Salmonella antibody was immobilized on one gold. surface of the quartz crystal with protein A. Salmonella detection was made by measuring resonant frequency shift owing to a mass change by specific binding of microbial cells to the gold surface of the PZ crystal. The PZ antibody sensor was operated optimally at 0.1M phosphate buffer, pH 7.2 and $35^{\circ}C$. The sensor was quite specific to Salmonella spp. The obtained frequency shift was correlated with the Salmonella concentration in the range of $10^5{\sim}10^6\;CFU/mL$. The frequency shift increased further by addition of polystyrene beads. The Salmonella detection which is indicated by a steady-state microbial adsorption to the quartz crystal was accomplished within 50min.

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Development of Liposome Immunoassay for Salmonella spp. using Immunomagnetic Separation and Immunoliposome

  • Shin, Jung-Hee;Kim, Myung-Hee
    • Journal of Microbiology and Biotechnology
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    • v.18 no.10
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    • pp.1689-1694
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    • 2008
  • The ability to detect Salmonella spp. is essential in the prevention of foodborne illness. This study examined a Salmonella spp. detection method involving the application of immunomagnetic separation and immunoliposomes (IMS/IL) encapsulating sulforhodamine B (SRB), a fluorescent dye. A quantitative assay was conducted by measuring the fluorescence intensity of SRB that was produced from an immunomagnetic bead-Salmonella spp.-immunoliposome complex. The results indicated detection limits of $2.7{\times}10^{5}$ and $5.2{\times}10^{3}$ CFU/ml for Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) and Salmonella enterka subsp. enterka serovar Typhimurium (S. Typhimurium), respectivley. The signal/noise ratio was improved by using 4% skim milk as a wash solution rather than 2% BSA. In addition, higher fluorescence intensity was obtained by increasing the liposome size. Compared with the conventional plating method, which takes 3-4 days for the isolation and identification of Salmonella spp., the total assay time of to h only including 6 h of culture enrichment was necessary for the Salmonella detection by IMS/IL. These results indicate that the IMS/ IL has great potential as an alternative rapid method for Salmonella detection.

Rapid Detection of Salmonella spp. by Antibody-Immobilized Piezoelectric Crystal Biosensor (고정화법을 달리하여 제조한 압전류적 항체 센서에 의한 Salmonella spp.의 신속 검출)

  • 박인선;김우연;김남수
    • Journal of Food Hygiene and Safety
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    • v.13 no.3
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    • pp.206-212
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    • 1998
  • An improved antibody-coated sensor system based on quartz crystal microbalance was developed for the detection of Salmonella spp. An antibody against Salmonella common structural antigen was immobilized onto one gold electrode of the piezoelectric quartz crystal surface by various immobilization procedures. The best results in sensitivity and stability were obtained with the thin layers of protein A and 3,3'-dithiopropionimidate.2HCI(DTBP), a homobifunctional thiol-cleavable crosslinker. After the addition of a S. typhimurium suspension into a reaction cell with 0.1 M sodium phosphate buffer, pH 7.2, the resonant frequency owing to S. typhimurium adsorption decreased conspicuously. The antibody-immobilized crystals prepared by the gold-protein A complex formation and DTBP thiolation showed the frequency shifts of 80 and 283 Hz, respectively. The time required for maximum frequency shift was about 30~60 min. The antibody-coated crystal could be reused for 6~8 consecutive assays.

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Phenotypic and Genotypic Characterization of Salmonella spp. Isolated from Pigs and their Farm Environment in Korea

  • Lim, Suk-Kyung;Byun, Jung-Ryul;Nam, Hyang-Mi;Lee, Hee-Soo;Jung, Suk-Chan
    • Journal of Microbiology and Biotechnology
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    • v.21 no.1
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    • pp.50-54
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    • 2011
  • This study's objective was to determine the prevalence of Salmonella spp. in pigs and their farm environments in Korea, and to investigate the relationship between the strains based on their phenotypic and genotypic characteristics. A total of 36 Salmonella spp. were isolated in this study: 18 isolates from 492 pigs (3.7%) and 18 isolates from 418 (4.3%) farmhouse environmental samples from 16 different pig farms. Of the Salmonella strains isolated from the numerous environmental samples, the highest prevalence was observed in slurry or manure, followed by partitions, farmer's hands, floors, water/nipples, ventilation sources, and feed, respectively. All the Salmonella isolates originating from different farms were genetically distinct. In three farms, however, identical phage types and pulse-field gel electrophoresis patterns were observed among Salmonella isolates from pig feces and environmental samples. This study suggests that environments contaminated with Salmonella could pose an infection risk to pigs on pig farms.

A Study on the Salmonella spp. isolation method in the sewage specimens (하수중의 Salmonella spp. 분리방법에 관한 비교연구)

  • 김성수;염종화
    • Journal of environmental and Sanitary engineering
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    • v.10 no.3
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    • pp.35-44
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    • 1995
  • This study was carried out in order to compare the methods for Salmonella spp. isolation from sewage and to settle the most appropriate conditions for that isolation. The direct enrichment method was more effective than the pre- enrichment method for Salmonella spp. isolation. The rate of detection was much higher when the specimens were enriched at $41.5^{\circ}C$ than when at $35^{\circ}C$. Usage of XLBG agar medium showed better results for Salmonella isolation than that of SS medium. It can be suggested that the most effective combination for Salmonella spp. isolation was the direct centrifugation(3,000 rpm 100m1)- direct enrichment($41.5^{\circ}$C)-usage of XLBG medium.

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Development of a Panel of Multiplex Real-Time Polymerase Chain Reaction Assays for Simultaneous Detection of Canine Enteric Bacterial Pathogens (개의 장내 병원균의 동시 검출을 위한 다중 실시간 중합효소연쇄반응분석 패널개발)

  • Jang, Hye-Jin;Han, Jae-Ik;Kang, Hyo-Min;Na, Ki-Jeong
    • Journal of Veterinary Clinics
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    • v.32 no.2
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    • pp.154-157
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    • 2015
  • A major cause of diarrhea in a dog is an infection with bacteria which include Salmonella spp., Campylobacter (C.) spp., and Clostridium (Cl.) spp.. It is fastidious to identify these bacteria by the culture. The purpose of this experiment is to devise the method for detecting Cl. perfringens, C. jejuni, C. coli, and Salmonella spp. with rapid and high sensitivity. The fecal samples collected from 71 normal and 66 diarrheic dog feces were used to compare the prevalence of the enteric pathogens and to develop a multiplex real-time polymerase chain reaction (PCR) assay for clinical use. Detection of Cl. perfringens, C. coli, and C. jejuni in diarrhea feces was higher than normal feces. A developed multiplex real-time PCR is useful for determining the presence and quantity of pathogen-specific or other unique sequences with in a fecal sample.

Prevalence of Antibiotic Resistant Foodborne Bacteria Isolated in Korea

  • Chung, Yun-Hee;Kim, Soo-Young;Chang, Yun-Hee
    • Food Science and Biotechnology
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    • v.14 no.2
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    • pp.216-222
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    • 2005
  • This study was performed to determine the antibiotic susceptibility profiles of Salmonella spp., coliforms, Listeria monocytogenes, Staphylococcus aureus and Vibrio spp. isolated from broiler carcasses, aquacultured flounders, hamburgers, and lettuce, which are foods consumed in large quantities in Korea. Salmonella spp. and L. monocytogenes were isolated only from broiler carcasses and Salmonella spp. had a high multidrug resistance rate of 61.1%. Meanwhile, coliforms and S. aureus were isolated from all four foods tested in this experiment. The multidrug resistance rate of coliforms from broiler carcasses was 50%, and that of Vibrio spp. from flounders was 71.4%. The resistance to tetracycline, streptomycin, ampicillin or carbenicillin was common regardless of the kind of food or isolate.

Primers for Typing Salmonella spp. using Random Amplified Polymorphic DNA (RAPD) Analysis (Salmonella spp.의 RAPD Typing을 위한 Primer의 분리력 비교)

  • Lim, Hyung-Kum;Lee, Kyung-Hee;Hong, Chong-Hae;Park, Gyung-Jin;Choi, Weon-Sang
    • Journal of Food Hygiene and Safety
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    • v.18 no.4
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    • pp.224-228
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    • 2003
  • Random amplified polymorphic DNA (RAPD) analysis is based on the amplification of random DNA segment using a single arbitratrary primer. For typing Salmonella spp., polymorphic DNA patterns identified by this method can be used. To select the primers for RAPD typing Salmonella spp., the performances of 20 primers were compared by analyzing 16 Salmonella spp. reference strains. Reproducible electrophoresis patterns were obtained. Among the 20 primers tested, 4 primers (A, OPG04, OPG10, OPL03) showed better differentiation than the others. At the time discrimination index, band clarity, band number and difficulty of band scoring were considered. These primers will be useful for typing Salmonella spp. in the future. Curretly, we are under investigation for the RAPD typing of contaminated Slmonella spp. for the risk analysis of pork processing plant using the primers.