• Title, Summary, Keyword: Salvia plebeia R. Br.

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Antioxidative and Anti-Inflammatory Activities of Salvia plebeia R. Br Extracts (배암차즈기(Salvia plebeia R. Br.)의 항산화 활성 및 항염증효과)

  • Lee, Yeong-Ye;Kang, Soon Ah
    • The Korean Journal of Food And Nutrition
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    • v.33 no.5
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    • pp.483-492
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    • 2020
  • This study provides data to explore functional medicinal food materials that can prevent adult diseases, and verified antioxidant and anti-inflammatory of each solvent fraction of the methanol extract of Salvia plebeia R. Br. in Korea. In the analysis of total phenol content, DPPH radical scavenging ability, and FRAP reduction ability as indicators of antioxidant activity, the methanol fraction and ethyl acetate fraction of the Salvia plebeia R. Br. group showed high antioxidant activity. Ethyl acetate fraction of Salvia plebeia R. Br. methanol extract also showed excellent antioxidative activity as compared with BHT. In the mouse macrophage line Raw 264.7 cells, the NO production ability by LPS treatment was significantly increased in the LPS treatment group compared to the untreated group. In inflammatory reactions induced by LPS treatment in Raw 264.7 cells, inflammatory cytokines (TNF-α, PGE2, IL-1β) and NO production were decreased in the EtOAc fraction and MeOH fraction of the methanol extract of Salvia plebeia R. Br. compared to the case of LPS treatment alone. The anti-inflammatory effect was proved by significantly inhibiting the production of inflammatory cytokines. The present results suggest that Salvia plebeia R. Br. supplementation is beneficial for the suppression of antioxidant and anti-inflammation.

Dyeing Properties and Functionality of Cotton Dyed with Extract from Spirodela polyrhiza and Mixture Extracts from Spirodela polyrhiza and Salvia plebeia R. Br. (개구리밥 추출물 및 개구리밥과 곰보배추 혼합 추출물로 염색한 면직물의 염색성 및 기능성)

  • Jung, Jin-Soun;Jang, Hyun-Joo
    • Fashion & Textile Research Journal
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    • v.18 no.6
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    • pp.869-877
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    • 2016
  • This study looks into the dyeing properties and functionality of cotton fabrics dyed in both the Spirodela polyrhiza extract and the extract resulting from the mixture of Salvia plebeia R. Br. and Spirodela polyrhiza. Since the UV-Vis Spectrum of the methanol extract of Spirodela polyrhiza shows absorption peaks at 256, 268nm, and 345nm, it can be inferred that the compound that Spirodela polyrhiza contains is a flavonoid. In addition, it can also be presumed that, by analyzing the infrared absorption spectrum of Spirodela polyrhiza, the plant contains flavonoid compounds, just like Salvia plebeia R. Br.. The UV protection factors of the cotton fabrics dyed in both the Spirodela polyrhiza extract and the extract from the mixture of Salvia plebeia R. Br. and Spirodela polyrhiza were 50+, presenting outstanding UV protection factors. The deodorization rate of the cotton dyed in the Spirodela polyrhiza extract was between 30 and 120 minutes, and the rate rose from 92% to 97% as time passed. The deodorization rate of the cotton dyed in the extract from the mixture of Salvia plebeia R. Br. and Spirodela polyrhiza increased from 88% to more than 91%. The result also revealed that overall the fastness of color, including color fastness to washing related to change in color, as well as the color fastness to light of the fabric dyed in the extract from the mixture of the two plants improved, compared to the cloth dyed only in Spirodela polyrhiza extract. Furthermore, the antibacterial activity was also strengthened.

Analytical Method for the Validation of Hispidulin as a Marker Compound for the Standardization of Salvia plebeia R. Br. Extracts as a Functional Ingredient (배암차즈기 추출물의 기능성원료 표준화를 위한 지표성분으로서 Hispidulin의 분석법 평가)

  • Jeon, Yoon Jung;Kwak, Hoyoung;Choi, Jong Gil;Lee, Je Hyuk;Choi, Soo Im
    • Korean Journal of Medicinal Crop Science
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    • v.24 no.4
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    • pp.271-276
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    • 2016
  • Background: In the present study, we established an HPLC (high performance liquid chromatography)-analysis method for the determination of marker compounds as a part of the material standardization for the development of health-functional foods from Salvia plebeia R. Br. extract. Methods and Results: The quantitative determination method of hispidulin as a marker compound was optimized by HPLC analysis using a YMC hydrosphere C18 column with a gradient elution system. This method was validated using specificity, linearity, accuracy, and precision tests. It showed a high linearity in the calibration curve with a coefficient of correlation ($r^2$) of 0.999995. The method was fully validated, and was sensitive, with the limit of detection (LOD) at $0.09{\mu}g{\cdot}m{\ell}^{-1}$ and limit of quantification (LOQ) at $0.27{\mu}g{\cdot}m{\ell}^{-1}$. The relative standard deviation (RSD) values of the data from intra- and inter-day precision were 0.05 - 0.22% and 0.32 - 0.42%, respectively, and the intra- and inter-day accuracy of hispidulin were 99.5 - 102.3% and 98.8 - 101.5%, respectively. The average content of hispidulin in Salvia plebeia R. Br. extract was $3.945mg{\cdot}g^{-1}$ (0.39%). Conclusions: These results suggest that the developed HPLC method is very efficient, and that it could contribute to the quality control of Salvia plebeia R. Br. extracts as a functional ingredient in health functional foods.

A Case of Hepatotoxicity by Salvia Plebeia R. Br.

  • Son, Chang-gue
    • The Journal of Internal Korean Medicine
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    • v.40 no.6
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    • pp.1219-1224
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    • 2019
  • Objective: To investigate the possibility of hepatotoxicity by supplemental foods or folk herbs such as Salvia Plebeia R. Br. Methods: A hospitalized male patient with alcoholic liver disease and electrolyte imbalance had recovered, and then followed by rapid hepatic serum enzymes after taking S. Plebeia. This study monitored the clinical outcome and biochemical parameters. Result: A 58-year male had drunk frequently, which led to alcoholic steatohepatitis and hospitalization. Two weeks after his discharge from hospital, he felt nausea, dizziness, and mild difficulties in speech and walking, resulting in re-hospitalization at the Korean Medical Hospital. The symptoms disappeared on correction of the electrolyte imbalance suspected to have been caused by severe sweating while working in the outdoors, and the patient was discharged. During treatment and monitoring of his health as an outpatient, the serum hepatic enzyme rapidly elevated approximately 10-fold in hepatic enzymes; the enzyme levels fluctuated according to whether or not he was taking the boiled water of S. Plebeia. The RUCAM score was 12, which met the criteria for toxic hepatitis by S. Plebeia. His general condition and abnormal hepatic enzymes recovered with cessation of S. Plebeia and administration of Chungganplus syrup (CGX). Conclusion: This study reports the hepatotoxic risk of Salvia Plebeia, which is commonly used as a folk remedy in Korea.

Antiadipogenic Effects of Salvia plebeia R. Br. Extracts by Extraction Conditions in 3T3-L1 Preadipocytes (추출조건에 따른 배암차즈기 추출물의 지방세포 분화 및 지방축적 억제 효과)

  • Choi, Soo Im;Kwak, Hoyoung;Kim, Jae Yoon;Choi, Jong Gil;Lee, Je Hyuk
    • Korean Journal of Medicinal Crop Science
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    • v.23 no.3
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    • pp.245-252
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    • 2015
  • This study was carried out to investigate the effects of Salvia plebeia R. Br. ethanolic extract with different aspects (stem/leaf and whole plant) on differentiation and lipid accumulation in 3T3-L1 preadipocytes. The morphological changes and the degrees of lipid accumulation in 3T3-L1 cells were measured by Oil Red O staining and intra-cellular triglyceride (TG) assay. The mRNA expressions of special peroxisome proliferation activated receptor- genes (PPAR), CCAAT/ enhancer-binding protein (C/$EBP{\alpha}$), fatty acid synthase (FAS) and lipoprotein lipase (LPL) were detected by reverse transcriptase polymerase chain reaction (RT-PCR). The 50% ethanolic extracts ($100{\mu}g/mL$) of stem and leaf (SALE) and 30% ethanolic extracts (100 g/mL) of whole plant (SAE) from Salvia plebeia R. Br. were significantly attenuated lipid accumulation during adipogenesis in 3T3-L1 cells. Ethyl acetate-soluble fractions ($50{\mu}g/mL$) significantly inhibited lipid droplet accumulation in 3T3-L1 cells. In addition, SALE induced down-regulation of specific adipogenic transcriptional factors (C/$EBP{\alpha}$ and $PPAR{\gamma}$) and target genes (FAS and LPL) during adipogenesis. Salvia plebeia R. Br. may be used as a safe and efficient natural substance to manage obesity.

Cytotoxicity and Antimicrobial Effect of the Extract of Salvia plebeia (배암차즈기 추출물의 세포독성과 항균효과)

  • Shin, Min-Kyo;Kim, Seok-Keun;Lee, Sang-kon;Gang, Yeong-Seong;Kim, Seong-Su;Yang, Eun-Yeong;Lee, Hyun-Ok;Baek, Seung-Hwa
    • Korean Journal of Pharmacognosy
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    • v.32 no.1
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    • pp.55-60
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    • 2001
  • The comparison of $IC_{50}$ values of Salvia plebeia R. Br. extracts on L1210, $P388D_1$ cancer and Vero normal cell lines showed that the n-hexane soluble extract of S. plebeia R. Br. retains the most growth-inhibitory activity against tumor cell lines. The minimum inhibitory concentration (MIC) of the extract against microorganisms were also examined. Antimicrobial activity of amocla and ketoconazole as references was compared to those of other solvent extracts such as $H_2O$, n-hexane, chloroform, ethyl acetate and methanol. The antimicrobial activity of extract had growth inhibition activity against gramnegative bacteria, gram-positive bacteria and fungi $(MIC\;>\;200\;{\mu}g/ml)$ except for n-hexane extract. Seven bacterial strains were tested for in vitro susceptibility to the extract of S. plebeia R. Br. However the n-hexane extract of S. plebeia R. Br. inhibited the growth of several bacterial strains (MIC values between 100 and $200\;{\mu}g/ml$ for gram positive bacteria, $25\;{\mu}g/ml$ for P. putida).

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Enhancement of Respiratory Protective and Therapeutic Effect of Salvia plebeia R. Br. Extracts in Combination with Korean Red Ginseng (배암차즈기와 홍삼 복합물의 호흡기 보호 및 질환 치료 상승 효과)

  • Shin, Han Jae;Gwak, Hyo Min;Lee, Moon Yong;Kyung, Jong Su;Jang, Kyoung Hwa;Han, Chang Kyun;Yang, Won Kyung;Kim, Seung Hyung
    • Korean Journal of Medicinal Crop Science
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    • v.27 no.3
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    • pp.218-231
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    • 2019
  • Background: We recently reported that Salvia plebeia R. Br. extracts suppress leukotriene production and effectively inhibit the airway inflammatory response by modulating inflammatory chemokine and cytokine expression. Here, we investigated the synergistic airway anti-inflammation effect of Salvia plebeia and Panax ginseng (Korean red ginseng, KRG) that has been used to treat various immune diseases such as asthma. Methods and Results: To evaluate the synergistic airway anti-inflammatory effect of Salvia plebeia and KRG, we measured the inhibitory effect of monotheraphy with either or co-theraphy with both on leukotriene and reactive oxygen species (ROS) production. Using coal a combustion, fly ash, and diesel exhaust particle (CFD)-induced respiratory disease mouse model, we found that co-theraphy synergistically suppressed airway inflammatory signs such as alveolar wall thickness and collagen fibers deposition, and decreased the number of total cell, $CD11b^+Gr-1^+$ cells, and inflammatory cytokines (IL17A, TNF, MIP-2 and CXCL-1) in bronchoalveolar lavage (BAL) fluid. Conclusions: We confirmed respiratory protection as a therapeutic effect of the Salbia plebeia-KRG 3 : 1 complex (KGC-03-PS) via anti-tracheal muscle contraction and expectorant animal studies using a CFD-induced respiratory disease mouse model.

Anti-Inflammatory Activity of Three Kinds of Salvia and Its Active Compounds (3종의 배암차즈기에 대한 염증억제 활성 비교 및 활성성분 구명)

  • Shin, Han Jae;Gwak, Hyo Min;Jang, Mi;Park, Si Hwan;Min, Hye Jeong;Lee, Jeong Min;Lee, Moon Yong;Kim, Jong Han;Kim, Sung Won;Han, Chang Kyun;Kim, Seung Hyung
    • Korean Journal of Medicinal Crop Science
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    • v.24 no.5
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    • pp.401-407
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    • 2016
  • Background: Salvia has been widely cultivated for use in flavoring and folk medicines in many countries, including Korea and China. In this study, we investigated the anti-inflammatory activity and the underlying active compounds of Salvia extract and its fractions. Methods and Results: The anti-inflammatory activity was measured by assessing the inhibition of cysteinyl leukotriene production in rat basophilic leukemia (RBL)-2H3 mast cells. Salvia plebeia R. Br. was found to have the most potent inhibitory activity on leukotriene production than S. japonica and S. chanroenica had. Fifty percent ethanol extracts of S. plebeia R. Br. were successively partitioned with n-hexane, methylene chloride, ethyl acetate, 1-butanol and water. The ethyl acetate (EtOAc) fraction showed stronger anti-inflammatory activity than other solvent fractions did. The EtOAc fraction was subjected to silica gel column chromatography elution with a chloroform and methanol gradient system (100 : 1 ${\rightarrow}$ 1 : 1) yielding 10 fractions. Three kinds of fractions (chloroform:methanol = 20 : 1, 10 : 1 and 5 : 1) showed high inhibitory activity on leukotriene production. We confirmed the major compounds with anti-inflammatory activity from S. plebeia R. Br. Conclusions: In this study, the major components of S. plebeia that showed leukotriene production inhibitory activity were isolated using solvent extraction and silica gel column chromatography. Rosmarinic acid, hispidulin and luteolin were identified as the major compounds with anti-inflammatory effect.

Anti-Inflammatory Activity of Salvia plebeia R. Br. Leaf through Heme Oxygenase-1 Induction in LPS-Stimulated RAW264.7 Macrophages (RAW264.7 대식세포에서 Heme Oxygenase-1 발현을 통한 배암차즈기(Salvia plebeia R. Br.) 잎 추출물의 항염증효과)

  • Jeong, Hye-Rim;Sung, Mi-Sun;Kim, Yung-Hwa;Ham, Hyeon-Mi;Choi, Young-Min;Lee, Jun-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.7
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    • pp.888-894
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    • 2012
  • Salvia plebeia R. Br. (Labiatae), distributed in many countries such as Korea, China, India, Iran, and Australia, is used as a folk remedy for a variety of inflammatory diseases including hepatitis, cough, diarrhea, gonorrhea, menorrhagia, tumors, and hemorrhoids. This study focused on determining the involvement of anti-inflammatory heme oxygenase-1 (HO-1) in the inhibitory activity of an extract of Salvia plebeia R. Br. leaves (SPL) on nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. SPL extract at the highest concentration (500 ${\mu}g/mL$) significantly inhibited NO production by approximately 85% and suppressed iNOS protein expression by approximately 90% compared to LPS-stimulated cells. The SPL extract induced the expression of HO-1 in a dose-dependent manner, and blocking HO-1 activity abolished the inhibitory effects of the SPL extract on NO production. These results suggest that an SPL extract has potent anti-inflammatory activity through HO-1 induction in RAW264.7 macrophages.

Isolation of Phytochemicals from Salvia plebeia Using Countercurrent Chromatography Coupled with Reversed-phase HPLC

  • Kil, Hyun Woo;Rho, Taewoong;Seo, Young Ju;Yu, Aram;Yoon, Kee Dong
    • Natural Product Sciences
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    • v.26 no.3
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    • pp.236-243
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    • 2020
  • Salvia plebeia R. Br. is a plant which has been used as an edible crop and traditional medicine in Asian countries. In this study, HPLC-PDA analysis and countercurrent chromatography (CCC) coupled with reversed-phase (RP) HPLC method were applied to isolate ten isolates from 3.3 g of n-butanol soluble extract from hot-water extract of S. plebeia. The use of CCC enabled us to efficiently fractionate the starting material with less sample loss and facilitate the isolation of compounds from S. plebeia extract using RP-HPLC. The isolates were determined to be caffeic acid (1), 6-hydroxyluteolin 7-O-β-D-glucoside (2), eudebeiolide B (3), (R)-rosmarinic acid (4), homoplantaginin (5), eudebeiolide D (6), plebeiolide C (7), salpleflavone (8), eupafolin (9) and hispidulin (10) based on the spectroscopic evidence.