• Title, Summary, Keyword: Sarcoma-180

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Inhibition of Tumor Formation and Changes in Hepatic Enzyme Activities by Kimchi Extracts in Sarcoma-180 Cell Transplanted Mice

  • Hur, Young-Mi;Kim, So-Hee;Park, Jong-Won;Park, Kun-Young
    • Preventive Nutrition and Food Science
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    • v.5 no.1
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    • pp.48-53
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    • 2000
  • Inhibitory effects of the methanol extract, hexane extract, methanol soluble fraction (MSF) and juice from 3 weeks fermented Kimchi on the tumor formation in sarcoma-180 cell transplanted mice were studied. Effects of the solvent extracts and juice of the Kimchi on the levels of lipid peroxide, glutathione, and the enzyme activities of the liver were also investigated in normal and sarcoma-180 cell transplanted mice. At 32 days following trans-plantation, MSF reduced the tumor formation by 54% compared with the control group, resulting in the smallest tumor weight. Lipid peroxided content in liver increased by the transplantation of sarcoma-180 cells. However, it decreased when MSF of Kimchi was treated to the mice. MSF also suppressed xanthine oxidase activity in cytosol of the liver cells in mice transplanted by sarcoma-180 cells. Kimchi extracts had no inhibitory effect on hepatic aminopyrine-N-demethylase activity in sarcoma-180 cell transplanted or normal mice. Methanol extract and hexane extract of Kimchi slightly increased hepatic glutathione contents in sarcoma-180 treated mice. The injection of MSF from Kimchi markedly increased glutathione levels in the liver of sarcoma-180 treated mice. The injection of MSF from Kimchi markedly increased glutathione levels in the liver of sarcoma-180 treated mice compared to the controls. The MSF recovered the activities of hepatic glutathione reductase and glutathione S-transferase that decreased by the injection of sarcoma-180 cells. These results showed that MSF of Kimchi could suppress the growth of tumors, inhibiting lipid peroxide production and xanthine oxidase activity, in mice. We also suggested that Kimchi extract might play an important role in the prevention of cancer by enhancement of the glutathione level itself as well as via glutathione reductase and glutathione S-transferase.

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On the Change of Blood and Serological Pictures of the Sarcoma-180 Mouse and that Mouse treated with Korean Ginseng Extracts (肉腫癌쥐와 高麗人蔘을 處理한 肉腫癌쥐의 血液學的 變化에 관하여)

  • 이유윤
    • The Korean Journal of Zoology
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    • v.23 no.4
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    • pp.229-238
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    • 1980
  • The present experiment was carried out to observe the effect of Korean ginseng extract on the blood picture of mice that have been infected with Sarcoma-180. The observation of standard group was made for 2 days, 7 days, 12 days, 15 days after the injection of Sarcoma-180 and the observation of ginseng extract group was made at 15 days after the injection of Sarcoma-180 and simultaneously with oral adminstration of ginseng extract every day for 15 days. 1. For 15 days after the injection of Sarcoma-180 in the standard group, the numbers of R.B.C. and platlet were not changed significantly, but the number of W.B.C. increased at the first stage, and then decreased suddenly after the middle stage. However, the number of W.B.C. was not severely decreased in the ginseng extract group. This phenomenon is estimated that ginseng extract assisted for W.B.C. to protect living body. 2. Serum protein picture of standard group was appeared as a nonselective protein losing pattern, and ginseng extract group was likely that of standard group, but it was not severe than that of standard group. This phenomenon is believed that ginseng extract gave some delay or immunization for Sarcoma-180 mouse against the development of Sarcoma-180. 3. Serum cholesterol quantities of standard group increased gradually until middle stage, but decreased in the last stage. But serum cholesterol quantity of ginseng extract group showed a slight increase. What the decrease of cholesterol quantity in the last stage of standard group is caused especially by the decreased intaking of feed among many causes, it may be the cause of intaking not decrease of feed in ginseng extract group, as the ginseng extract gave the Sarcoma-180 mouse some resistance against the development of Sarcoma-180. 4. Along with developing of Asrcoma-180 in the standard group, serum total lipid quantities increased, and $\\alpha-, \\beta$-lipoprotein quantities showed decrease phenomenon, but pre $\\beta$-lipoprotein quantities increased rapidly. The value of ginseng extract group was appeared the value in the first stage of standard group. This phenomenon was also estimated that ginseng extract protected the living body according to above theories, and that ginseng extract gave the Sarcoma-180 mouse some resistance against the development of Sarcoma-180.

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The Effect of Doenjang (Korean Soy Paste) on the Liver Enzyme Activities of the Sarcoma-180 Cell Transplanted Mice

  • Kim, Moon-Kyung;Moon, Suk-Hee;Park, Jong-Won;Park, Kun-Young
    • Preventive Nutrition and Food Science
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    • v.4 no.4
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    • pp.260-264
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    • 1999
  • Korean traditional fermented soy paste (doenjang) prolonged the life span of Balb/c mice injected with the sarcoma-180 cells. The activities of liver enzymes, such as xanthine oxidase, aminopyrine N-demethylase, aniline hydroxylase, ${\gamma}$-glutamylcysteine synthetase, glutathione reductase and glutathione S-transferase (GST), and the contents of lipid peroxide and glutathione were determined from the sarcoma-180 cell injected mice that were treated with methanol extracts from doenjang, miso and soybean. The content of lipid peroxide and the activity of xanthine oxidase in the liver of Balb/c mice which were increased by the transplantation of the sarcoma-180 cells were decreased by treatment with the methanol extract from doenjang. But the activities of aminopyrine N-dementhylase and aniline hydroxylase were not affected by the treatment of methanol extracts from doenjang to the mice injected with the sarcoma-180 cells. The content of glutathione, the activities of glutamylcysteine synthetase, glutathione reductase and glutathione S-transferase decreased by the injection of the sarcoma-180 were recovered considerably by the treatment of the methanol extract from doenjang.

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Antineoplastic natural products and the analogues V - Antitumor Activity of Skullcapflavon II

  • Ryu, Sung-Ho;Ahn, Byung-Zun;Pack, Moo-Young
    • Archives of Pharmacal Research
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    • v.8 no.4
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    • pp.253-256
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    • 1985
  • The effect of skullcapflavon II, 5, 2'- dihydroxy-6, 7, 8, 6'-tetramethoxyflavone, on the growth of transplantable L 1210 and sarcoma 180 tumors in mice was studied. Intraperitional treatment of skullcapflavon II cased a significant (T/C = 166%) and a moderate (T/C = 122 %) prolongations of the life spans of ICR and $BDF_{1}$ mice respectively, which had been intraperitioneally inoculated with sarcoma 180 and L 1210 cells. Peritumoral injection of skullacapflavon II on the solid form of sarcoma 180 in mice inhibited the tumor growth strongly (Inhibition rate = 71%).

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Antitumor Activity of Lactobacillus casei against Sarcoma 180 and Lewis Lung Carcinoma in Mice (생쥐에서 Sarcoma 180 및 Lewis Lung Carcinoma에 대한 Lactobacillus casei의 항암 효과)

  • 배형석;백영진;윤영호
    • Microbiology and Biotechnology Letters
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    • v.21 no.3
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    • pp.247-255
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    • 1993
  • Antitumor activity of Lactobacillus casei YIP 9018(LC9018) was studied in mice by using sarcoma 180(S-180) and Lewis lung carcinoma (3LL). Following the eatablishment of in vivo tumor models such as ascites form S-180, solid form S-180 and 3LL for estimating antitumor activity of Lactobacilli, optimal dose and injection route of heat-killed LC9018 for supperssion of local tumor were examined. Administration of 100ng/mouse of LC9018 significantly inhibited the growth of ascites form S-180, solid form S-180 and 3LL.

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Antitumor and Immuno-potentiating Activity against Mouse Sarcoma 180 by Crude Polysaccharides Extracted from Fruiting Body of Tricholoma matsutake (송이(Tricholoma matsutake)의 자실체에서 추출한 조다당류가 생쥐의 Sarcoma 180에 미치는 항암 및 면역증강 작용)

  • Hur, Hyun;Choi, Yon-Il;Lee, Tae-Soo
    • Journal of Life Science
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    • v.18 no.9
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    • pp.1290-1298
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    • 2008
  • Tricholoma matsutake, one of edible and medicinal mushroom belonging to Tricholomaceae of Agaricales, has been known to contain some curing effect on gastric cancer and ulcer, and inhibitory effect on sarcoma 180 and Ehrlich sarcoma. Neutral salt soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from fruiting body of the mushroom. In vitro cytotoxicity tests, crude polysaccharides were not cytotoxic against cancer cell lines such as Sarcoma 180, HepG2, HT29 and NIH3T3 at the concentration of 2.0 mg/ml. Intraperitoneal injection with crude polysaccharides showed life prolongation effect of 23.4$\sim$37.2% in mice previously inoculated with Sarcoma 180. Fr. MeOH and Fr. HW exhibited the immuno-potentiating activity of B lymphocyte by increasing the alkaline phosphatase activity by 2.2$\sim$11.9 folds compared with control at the concentration of 0.2$\sim$0.5 mg/ml. In case of Fr. NaCl, the numbers of peritoneal exudate cells and circulating leukocytes were increased by 6.0 and 1.5 folds at the concentration of 50 mg/kg, respectively. Therefore, it is concluded that crude polysaccharides extracted from fruiting body of Tricholoma matsutake showed antitumor and immuno-potentiating activity against Sarcoma 180 of mouse.

Curcuma Longa L. Extract Controls Cancer Cell (Sarcoma 180) Growth (강황(薑黃) 추출물의 암세포 성장 억제 효과)

  • Cho, Su-In
    • The Korea Journal of Herbology
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    • v.20 no.4
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    • pp.27-31
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    • 2005
  • Objectives : The anticancer response of three different types of water extracts of Zingiberaceae Curcuma longa L. tested for sarcoma 180. Only few studies carried out to investigate the effects of other contents of Curcuma longa L. in anticancer activities, therefore, in this study we have investigated the effects of other component then curcumin in Curcuma longa L. for anticancer a activities. Methods : Three different types of water extracts of Curcuma longa L. were prepared as follows. The sarcoma cells (S180) were maintained in Dulbecco's modified Eagle's medium (DMEM) and were seeded on 24-well cell culture cluster flat bottom with lid tissue culture treated non-pyrogenic polystyrene. The growth of sarcoma 180 was monitored for 1, 2 and 5 days. The sarcoma cells were pictured using inverted microscope and cell density was counted using hemocytometry. Results : After 5 days in the culture medium the results showed high growth of sarcoma 180 for control condition and the surface of CCP plates were fully covered with the cells. In case of medium in which the 10% of filtered water extract of Curcuma longa L. was added a very limited growth of sarcoma 180 was observed. The results were showed only small difference in cell density for two different concentrations of unfiltered water extracts of Curcuma longa L. whereasin case of filtered water extracts the control of sarcoma growth shows better result. Conclusion : The filtered water extracts showed the best result relatively to the unfiltered water extracts for two different concentrations. This indicates that the water extracts of Curcuma longa L. can have anticancer activities possibly without curcumin.

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Antitumorigenic Effects of Tannin From Persimmon Leaves on Sarcoma 180-induced Tumor in Mice

  • Moon, Sung-Chai;Park, Kyong-Hee;Rhew, Tae-Hyong;Park, Kun-Young;Kim, Byeong-Gee
    • Preventive Nutrition and Food Science
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    • v.3 no.1
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    • pp.92-97
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    • 1998
  • The changes of morphology and protein pattern of sarcoma 180 cells treated with or without trannins extracted from persimmon leaves were evaluated by light microscopy, electrophoresis and Western blotting. The sarcoma 180 cells treated with tannins increased the amount of proteins which presumably were intermediate filament cytokeratins detected by electrophoresis and Western blot. Tannins was indirectly cytotoxic to the sarcoma 180 cells and increased the intermediate filament protein level in the cells.

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Experimental Study of Naesosan(內消散) on the Effects of Anti-Cancer (內消散의 抗癌效果에 관한 實驗的 硏究)

  • Park, Su-Yeon;Choe, Jeong-Hwa
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.14 no.1
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    • pp.154-166
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    • 2001
  • Naesosan(NSS) has been used in Oriental Medicine as a drug that treated carbuncle and cellulitis. So, the purpose of this Study was to investigate effects of NSS on the cytotoxicity of cancer cell lines and lymphocytes in vitro, proliferation of Ll210 cells and lymphocytes in L1210 cells transplanted mice, improvement of blood count in Ll210 cells transplanted mice, tumor weight and body weight in sarcoma-180 cells transplanted mice, survival prolongation in sarcoma-180 cells transplanted mice. We used NSS extract with freeze-dried, 8wks-old male mice(balb/c and ICR mouse $18{\pm}2g$). Ll210 cell lines, and sarcoma-180 cell lines for this Study, The proliferation of cells was tested using a colorimetric tetrazoliun assay(MTT assay). The results of this Study were obtained as follows ; 1. NSS showed significantly cytotoxicitic effects of cancer cell lines, did not show cytotoxicitic effects of lymphocytes. 2, Proliferation of lymphocytes in L1210 cells transplanted mice did not effects by NSS. 3. NSS inhibited significantly the proliferation of L1210 cells in L1210 cells transplanted mice. 4. NSS improved significantly the blood count in Ll210 cells transplanted mice. 5. NSS increased significantly th body weight in sarcoma-180 cells transplanted mice. 6. NSS dereased significantly the tumor weight in sarcoma-180 cells transplanted mice. 7. NSS prolonged significantly the survival time in sarcoma-180 cells transplanted mice.

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Antitumor Effect of Persimmon Leaves in vivo using Sarcoma-180 Cells (Sarcoma-180 세포를 이용한 in vivo에서 감잎의 항암효과)

  • 문숙희;김광혁;박건영
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.5
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    • pp.865-870
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    • 1996
  • Antitumor effects of hexane, chloroform fractions and tannins from persimmon leaves were studied by using sarcoma-180 tumor cells. The solid tumor growth was inhibited most effectively when 0.5mg/kg of hexane fraction was administerated to the Balb/c mouse. The life prolongation effects were 35.6%, 11.5% and 29.9%, respectively when hexane fraction, chloroform fraction and tannins from the persimmon leaves were administerated to the mouse. Spleen index, a marker for immunological activity, was increased in mice administrated hexane fraction, chloroform fraction and tannins of persimmon loaves, compared with the control group.

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