• Title, Summary, Keyword: Sargassum fulvellum

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Antioxidant Activity of Sulfated Polysaccharides Isolated from Sargassum fulvellum

  • Choi, Dae-Sung;Athukorala, Yasantha;Jeon, You-Jin;Senevirathne, Mahinda;Cho, Kyun-Rha;Kim, Soo-Hyun
    • Preventive Nutrition and Food Science
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    • v.12 no.2
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    • pp.65-73
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    • 2007
  • Sargassum fulvellum, a marine brown alga, is a popular low priced edible plant in Korean markets. The polysaccharide fraction of the alga was separated and investigated for its radical scavenging activities and the results compared with those of commercial fucoidans (Fucus vesiculosus and Undaria pinnatifida), BHA and ${\alpha}$-tocopherol. The polysaccharide fraction of S. fulvellum showed a promising DPPH radical scavenging activity than did other fucoidans. Moreover, the sample exhibited a dose-dependent activity on hydrogen peroxide scavenging activity in the V79-4 cell line. Interestingly, all the tested polysaccharide counterparts were more potent NO. scavengers than were the commercial antioxidants, BHA and ${\alpha}$-tocopherol. The sulfated polysaccharide of S. fulvellum had an approximate molecular weight of 529 kDa and mainly consisted of fucose and galactose, and minor amounts of mannose, rhamnose and xylose.

Biological Analysis of Enzymatic Extracts from Sargassum fulvellum Using Polysaccharide Degrading Enzyme (Polysaccharide Degrading Enzyme을 이용한 참모자반 효소분해 추출물의 생리활성 연구)

  • Cho, Eun Kyung;Kang, Su Hee;Choi, Young Ju
    • KSBB Journal
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    • v.28 no.6
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    • pp.349-355
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    • 2013
  • SC092 strain, producing a polysaccharide degrading enzyme, was isolated from the seawater. This strain was identified as Microbulbifer sp. using the comparative sequence analysis against known 16S rRNA sequence. A polysaccharide degrading enzyme from this strain was used to acquire the enzymatic extracts of Sargassum fulvellum. DPPH radical scavenging and SOD activity of the enzyme extracts of S. fulvellum were about 61.9% and 82.9% at 2 mg/mL, respectively. Nitrite scavenging activities was 52.5% at 2 mg/mL on pH 1.2. In addition, ${\alpha}$-glucosidase inhibitory activity was also increased in a dose-dependent manner and was about 52.7% at 2 mg/mL. To determine the influence of enzyme extracts of S. fulvellum on alcohol metabolism, the generating activity of reduced-nicotinamide adenine dinucleotide (NADH) by alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) were measured. ADH and ALDH activities were 118.0% and 177% at 2 mg/mL, respectively. ${\alpha}$-glucosidase inhibitory activity of enzyme extracts of S. fulvellum was remarkably increased in a dose-dependent manner and was about 52.7% at 2 mg/mL. These results indicate alcoholizing and ${\alpha}$-glucosidase inhibitory activities can be enhanced by the enzymatic extracts of S. fulvellum.

Lead Biosorption by Biosorbent Materials of Marine Brown Algae U. pinnatifida, H. fusiformis, and S. fulvellum

  • LEE Mingyu;KAM Sangkyu;LEE Donghwan
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.6
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    • pp.936-943
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    • 1997
  • Biosorbents of nonliving, dried marine brown algae Undaria pinnatifida, Hizikia fusiformis, and Sargassum fulvellum were investigated for their lead biosorption performances. As the amount of biosorbent added was increased, the lead removal by biosorbent materials increased but the lead biosorption capacities decreased. However the lead biosorption capacity by the biosorbent materials increased with increasing initial lead concentration and pH in the range of $C_o\;10\~500\;mg/L$. Among the biosorbent materials used in this study, the lead biosorption capacity in the solutions with no pH adjustment decreased in the following sequence: U. pinnatifida > H. fusiformis > S. fulvellum. Equilibrium parameters based on Langmuir and Freundlich isotherm were determined. It was found that the lead biosorption by biosorbent materials were expressed by the Langmuir isotherm better than the Freundlich isotherm.

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STUDIES ON THE EXTRACTION OF SEA WEED PROTEINS 2. Extraction of NaCl and Alcohol Soluble Proteins (해조단백질 추출에 관한 연구 2. 식염가용성 및 알콜가용성 단백질의 추출)

  • LEE Kang-Ho;RYU Hong-Soo;WOO Soon-Im
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.10 no.4
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    • pp.189-197
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    • 1977
  • In present study, the effects of various factors including the solvent concentration, extraction time and temperature, the ratio of sample vs extraction solvent, (w/v) and pH upon the extractability of the NaCl and alcohol soluble proteins of marine algae were investigated. Eight species of fresh algae, the major ones in consumption as food, namely Porphyra suborbiculata, Undarie pinnatifida, Hizikia fusiforme, Sargassum, fulvellum, Enteromorpha linza, Sargassum kjellmanianum, Codium coarctatum, and Ulva pertusa were used for the extraction of NaCl soluble protein and dried materials of four species, Perphyra suborbiculata, Undaria pinnatifida, Enteromorpha linza and Sargassum fulvellum were used for the extraction of alcohol soluble protein. The frozen and mascerated samples were prepared by the same method described in previous paper (Ryu, 1977). And the dried materials were moistened with alcohol solution before freezing. The effect of solvent concentration on the extractability of NaCl soluble protein differed from species. The extractability of Undaria Pinnatifide, Hizikia fusiforme, Perphyra suborbiculata, Enteromorpha linza, and Ulva pertusa reached maxima at 0.25M NaCl solution while the 1.0M for Sargassum fulvellum, Saygassum kjellmanianum and Codium coarctatum. In case of alcohol soluble proteins, it was shown at $20\%$ ethanol solution for Porphyra suborbiculata, Undaria pinnatifida, Enteromorpha linza, and Sargassum fulvellum. Variation of the ratio of sample vs solvent gave slight effect upon the extractability, but the ratio of 1:30(w/v) seemed most efficient for the extraction of NaCl soluble proteins and 100 ml solvent added to 1 g dried sample was effective in case of alcohol soluble proteins. Extraction time has a minimal effect upon the extraction of alcohol soluble protein, and approximately 21 to $43\%$ of algal protein was extracted within 1 hour. But in case of NaCl soluble protein extraction, the effect of time revealed differently from species to species resulting in that the extraction for 1 hour gave a maximum extractability in Ulva pertusa and Enteromorpha linza, 2 hours in Porphyra suborbiculata, Codium coarctatum and 3 hours in Undaria pinnatifica, Hizikia susiforme, Sargassum fulvellum and Sargassum kjellmanianum. When the NaCl soluble protein of Undaria pinnatifida and Enteromopha linza was extracted at various temperature, the most effective extraction temperature was $40^{\circ}C$ while the temperature was $50^{\circ}C$ for Undaria pinnatifida and $60^{\circ}C$ for Hixikia fusiforme, Sargassum fulvellum, Sargassum kjellmanianum and Codium coarctatum. Bus in case of alcohol soluble extraction, the optimum temperature was $30^{\circ}C$ for Enteromorpha linza and $40^{\circ}C$ for Undaria pinnatifida, Sargassum fulvellum and Porphyra suborbiculata. In the effect of pH on extractability, the maximum extractability of NaCl soluble proteins was obtained at pH 7to 8 and pH 8 to 9 for alcohol soluble protein.

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Evaluation of the Antioxidant and Antiproliferative Properties of a Hot-water Extract from Gulfweed, Sargassum fulvellum

  • Kim, So Jung;Kang, Mingyeong;Lee, Taek-Kyun
    • Journal of Marine Bioscience and Biotechnology
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    • v.10 no.2
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    • pp.53-61
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    • 2018
  • Sargassum fulvellum (gulfweed) is a widespread seaweed in the coastal areas of northeast Asia. In the present study, we identified the phenolic compounds present in aqueous and ethanolic extracts of S. fulvellum and evaluated their antioxidative properties and their abilities to block cell proliferation using in vitro assays: antioxidant activity was assessed by using a DPPH assay and superoxide anion scavenging activity, anti-tyrosinase activity, and anti-proliferative activity were assessed using MTT and lactate dehydrogenase [LDH] assays in vascular smooth muscle cells. The hot-water ($65^{\circ}C$) extract had a higher phenol content than the ethanolic extract. The hot-water extract showed a statistically significant increase in free radical scavenging activity and a greater ability to reduce proliferation of vascular smooth muscle cells stimulated with platelet-derived growth factor-BB. Taken together, hot-water extracts of S. fulvellum may be an important source of antioxidative and antiproliferative agents.

Growth, Maturation and Development of Sargassum fulvellum (Sargassaceae, Phaeophyta) (모자반(Sargassum fulvellum)의 생장, 성숙과 발생)

  • HWANG Eun Kyoung;BAEK Jae Min;PARK Chan Sun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.38 no.2
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    • pp.112-117
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    • 2005
  • Sargassum fulvellum (Turner) C. Agardh, an edible brown alga is farmed commercially by sexual reproduction and vegetative regeneration. Investigations were made on the phenology, abundance and maturity of reproductive structures in mature fronds, egg release and young germling development under different light conditions (20, 50, 80 and $100{\mu}mol/m^2/s$) and temperatures (5, 10, 15, 20 and $25^{\circ}C$). Monthly sampling was carried out by SCUBA diving at Chungsando on the southwestern coast of Korea from September 2002 to August 2003. The Maximum length of thalli was $104.6{\pm}20.7{\cal}cm$ in March 2003 when the water temperature was $9.0^{\circ}C$ and minimum was $0.8{\pm}0.5{\cal}cm$ in June when the water temperature was $19.5^{\circ}C$. Receptacle formation was observed from February to April. The peak period of egg release for this alga was in April when the water temperature was about $10^{\circ}C$ in nature. In the culture regimes of temperature and irradiance, the egg release of the excised female receptacle was highly affected by temperature. The maximum rate of egg release was $96.7{\pm}5.8{\%}$ under $20^{\circ}C$ and $80{\mu}mol/m^2/s$. The maximum length of young germlings was $3.9{\pm}0.2{\cal}mm$ after 35 days culture under $15^{\circ}C$ and $80{\mu}mol/m^2/s$.

Study on Growth Characteristics of Sargassum fulvellum in the Integrated Multi-trophic Aquaculture (IMTA) System

  • Kim, Young-Dae;Park, Mi-Seon;Min, Byung-Hwa;Jeong, Seong-Jae;Kim, Hyung-Chul;Yoo, Hyun-Il;Lee, Won-Chan;Choi, Jae-Suk
    • Journal of Environmental Science International
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    • v.23 no.10
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    • pp.1703-1718
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    • 2014
  • An eco-friendly integrated multi-trophic aquaculture (IMTA) farming technique was developed with the goal of resolving eutrophication by excess feed and feces as fish-farming by-products. A variety of seaweed species were tried to remove inorganic nutrients produced by fish farming. However, there have been few trials to use Sargassum fulvellum in an IMTA system, a species with a relatively wide distribution across regions with various habitat conditions, great nutrient removal efficiency and importance for human food source and industrial purposes. In this regard, our study tried to examine feasibility of using S. fulvellum in an IMTA system by analyzing growth characteristics of the species in an IMTA system comprising of rockfish (Sebastes shlegeli), sea cucumber (Stichopus japonocus) and the tried S. fulvellum (October 2011 - November 2012). We also monitored environment conditions around the system including current speed, water temperature and inorganic nutrient level as they may affect growth of S. fulvellum. S. fulvellum in the IMTA system, which were $15.72{\pm}5.67mm$ long at the start of the experiment in October 2011, grew to a maximum of $1093{\pm}271.13mm$ by May 2012. In September, seaweed growth was reduced to a minimum of $280{\pm}70.43mm$ in length. Then, S. fulvellum began to grow again reaching $325{\pm}196.19mm$ by November 2012. Wet weight of the seaweed was $4.01{\pm}1.89g$ at the start of the experiment and reached a maximum of $109.26{\pm}34.23g$ in May. The weight gradually declined to a low of $15.12{\pm}8.40g$ in September 2012. Weight began to increase once more, rising to $39.27{\pm}21.69g$ by November. During the experiment, the average velocity at the surface and the bottom was 6.5 cm/s and 3.4 cm/s, respectively. The water temperature ranged $5.0-23.5^{\circ}C$, which was considered suitable for growing S. fulvellum. Results of the study indicated no significant differences in inorganic nutrients between pre- and post-IMTA installation. It was thus concluded that S. fulvellum can be a suitable seaweed species to be used in an IMTA system.

Community Structure of Subtitdal Marine Algae at Uljin on theEast Coast of Korea (동해안 울진 연안 조하대 저서 해조류의 군집구조)

  • Choi, Chang-Geun;Kwak, Seok-Nam;Sohn, Chul-Hyun
    • ALGAE
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    • v.21 no.4
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    • pp.463-470
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    • 2006
  • Studies have been made of the species composition and variation of benthic marine algae at subtidal zone of Uljin on the east coast of Korea. Destructive method was employed to measure biomass over four seasons (2000-2002). Of 87 marine algae species identified, 11 were Chlorophyta, 29 were Phaeophyta and 47 were Rhodophyta. Dominant species in biomass were Ulva pertusa, Undaria pinnatifida and Sargassum piluliferum in summer, Laminaria religiosa, Sargassum fulvellum and Gigartina tenella in autumn, Codium fragile, Undaria pinnatifida, Sargassum horneri, S. piluliferum and S. ringgoldianum in winter, and Undaria pinnatifida, Dictyopteris divaricata, Sargassum confusum and S. horneri in spring. In general, green algae (Ulva pertusa, Codium fragile) and brown algae (Undaria pinnatifida, Sargassum fulvellum, S. horneri, S. piluliferum) appeared predominantly in the 3, 6 m depths and red algae (Gelidium amansii, Plocamium telfairiae) in the 9, 12 m depths. The barren ground of the rocky shore might provide the decrease of benthic marine algae biomass and species.

α-Glucosidase Inhibitory Effects for Solvent Fractions from Methanol Extracts of Sargassum fulvellum and Its Antioxidant and Alcohol-Metabolizing Activities (참모자반 메탄올 추출 분획물의 항산화 및 숙취해소능과 α-glucosidase 활성저해효과)

  • Kang, Su Hee;Cho, Eun Kyung;Choi, Young Ju
    • Journal of Life Science
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    • v.22 no.10
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    • pp.1420-1427
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    • 2012
  • We investigated the physiological activity and solvent-partitioned fractions of methanol extracts from the green seaweed Sargassum fulvellum. The methanol extract from S. fulvellum was sequentially fractionated with n-hexane (SFMH), methanol (SFMM), buthanol (SFMB), and water (SFMA). We investigated the antioxidant activities of solvent fractions from S. fulvellum by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity and an SOD activity assay. DPPH radical scavenging capacity of SFMM was 79.5% at 10 mg/ml. SOD activity of SFMM was 79.9% at 10 mg/ml. Nitrite scavenging activities of solvent fractions from S. fulvellum were investigated under different pH conditions and showed the most remarkable effect at pH 1.2. In particular, the activity of SFMB was higher than the other fractions. ADH activity and ALDH activity of SFMM were 177.0% and 167.4% at 10 mg/ml, respectively. ${\alpha}$-Glucosidase inhibitory activity of SFMH increased in a dose-dependent manner and was about 94.1% at 2 mg/ml. Elastase inhibitory activity was 93.2% at 2 mg/ml. These results revealed that S. fulvellum extracts have strong antioxidant and alcohol dehydrogenase activities and ${\alpha}$-glucosidase inhibitory activity, suggesting that S. fulvellum extracts have potential as a source of natural products for health and beauty.

Anti-inflammatory Activity of the Water Extract of Sargassum fulvellum (참모자반 (Sargassum fulvellum) 물 추출물의 염증 억제 활성)

  • Jeong, Da-Hyun;Kim, Koth-Bong-Woo-Ri;Kang, Bo-Kyeong;Jung, Seul-A;Kim, Hyun-Jee;Jeong, Hee-Ye;Bark, Si-Woo;Ahn, Dong-Hyun
    • KSBB Journal
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    • v.27 no.6
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    • pp.325-329
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    • 2012
  • The anti-inflammatory effects of Sargassum fulvellum water extracts (SFWE) were investigated using lipopolysaccharide (LPS)-induced inflammatory response in this study. To examine the potential anti-inflammatory properties of SFWE, the NO, TNF-${\alpha}$, IL-6, IL-$1{\beta}$, and cell proliferation were measured. It was confirmed that the NO and TNF-${\alpha}$ secretion were significantly suppressed when SFWE was added to LPS-stimulated RAW 264.7 cells. Moreover, the expression of IL-6 and IL-$1{\beta}$ cytokines was suppressed by SFWE in a dose-dependent manner. Especially, IL-6 inhibition activities were over 50% at 1% of SFWE. The cytotoxicity of SFWE and the proliferation of macrophages was measured by MTT assay. As a result, there was no cytotoxicity in the macrophage proliferation treated with SFWE compared to the control. In conclusion, these results suggested that the SFWE may have significant effects on inflammatory factors and can be a potential anti-inflammatory therapeutic materials.