• Title, Summary, Keyword: Science Culture

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Effect of chlorella culture solution using animal liquid manure on improving seed germination in perennial ryegrass

  • Lee, Jin Woong;Choi, Min Soo;Seo, Un Kab;Ryoo, Jong Won
    • Proceedings of the Korean Society of Crop Science Conference
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    • pp.212-212
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    • 2017
  • This experiments were conducted to evaluate the influence of Chlorella culture solution on seed germination of perennial ryegrass seeds. Chlorella are known to contain different bioactive compounds. In present research work, Chlorella culture solution using liquid manure as medium have been used to study their effects on germination and root length. The study conducted a germination experiment in petri-dishes. Four treatments were compared: non-treated control treated with distilled water, Chlorella culture solution and Chlorella culture filtrate, and liquid manure. The germination percentage of perennial ryegrass seeds was highest in the Chlorella culture solution treatment. Days required for 50, 70% seed germination was the fast in Chlorella culture solution and the Chlorella culture filtrate treatment. Root length of perennial ryegrass seeds was long by 1~2cm in the Chlorella culture solution compared with no treated control. The germination index of perennial ryegrass seeds was high by 180~202% in the Chlorella culture solution treatment compared to no treatment. Chlorella culture solution and the Chlorella culture filtrate have shown stimulatory effects in germination and development of root. Use of Chlorella culture solution and the Chlorella culture filtrate can be recommended to farmers as a ecofriendly practice for better germination and growth. Present research work reveals that Chlorella contain certain growth promoting substances which enhances seed germination.

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Critical Reconstruction of The Korean Science Culture (한국 과학문화의 비판적 재구성)

  • Lee Cho-Sik
    • Journal of Science and Technology Studies
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    • v.1 no.1
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    • pp.5-27
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    • 2001
  • This paper makes a preparatory attempt to reconstruct 'the Korean Science Culture' toward which I think the Koreans should direct and orient themselves rather than describe the history of the science culture in Korea. This task requires us to define 'science culture.' So I propose, based on the definitions of science culture so far made, to consider the model of 'Humanized Science Culture' as a regulating ideal of the Korean Science Culture. To support the model, analyzing the concept of 'science literacy' of the 2061 Project, I posit the model of KDSC (Korean Designer of Science Culture) as the Koreans who will actually come to design the Korean Science Culture. KDSC refers to 'a reasonable man' who will represent a desirable Korean, rather than to a natural Korean. Then I spell out the conditions of becoming a KDSC and scrutinize the philosophical backbones of those conditions. Finally, I make a few suggestions for developing a program of designing and implementing the Korean Science Culture from the viewpoint of a model of KDSC.

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Cultural Implications of Science and Technology (과학기술의 문화적 함의)

  • Lim Hy-Sop
    • Journal of Science and Technology Studies
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    • v.3 no.1
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    • pp.1-17
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    • 2003
  • Though science and technology can understood as a part of cultural system, the relationship of science/technology with culture is often viewed as contradictory or mutually exclusive by many scholars. Therefore, in the first section of the present paper, the nature of science and technology as a cultural form is examined. Here, science is viewed as a form of symbolic culture, while technology is regarded as a form of instrumental culture. In the second section, the contradictory relationship and/or tensions between science-technological system (instrumental culture) and cultural system (symbolic culture) within a society is discussed. In the final section of this paper, 'science culture' is conceptualized as a dynamic social process in which contradictory science sub-cultures of political system(state), economic system(cooperations), civil society (including academic community of scientists and other social organizations and movements), and cultural system are supposed to be coordinated. In conclusion, establishment of 'humanized science culture' and 'democratic science-culture movement' is proposed as an alternative way of resolving contradictory relationship between cultural system and science-technological system in the modern world.

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Biodegradation of 2,4,5-Trichlorophenol Using Cell-Free Culture Broths of Phanerochaete chrysosporium

  • Choi, Sueh-Yung;Moon, Seung-Hyeon;Lee, Jae-Suk;Gu, Man-Bock
    • Journal of Microbiology and Biotechnology
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    • v.10 no.6
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    • pp.759-763
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    • 2000
  • Cell-free culture broth of Phanerochaete chrysosporium has been adopted to biologically degrade 2,4,5-trichlorophenol. Two different medium compositions of nitrogen-sufficient and nitrogen-limited were compared for their distribution of isozymes, activity of lignin peroxidase, and production of oxalate. The two different culture broths were tested for their ability to degrade 2,4,5-trichlorophenol, and the biodegradation efficiency was estimated in terms of the disappearance of 2,4,5-trichlorophenol. The degradation efficiency for the nitrogen-limited culture broth was higher than that of the nitrogen-sufficient culture broth, since the nitrogen-limited culture broth induced lignin peroxidases (LiPs) and manganese peroxidases (MnPs), and contained sufficient oxalate for producing necessary radicals. Finally, the possible mechanism of 2,4,5-CP degradation using the nitrogen-limited culture broth was proposed.

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Effects of Suspension Culture on Proliferation and Undifferentiation of Spermatogonial Stem Cells Derived from Porcine Neonatal Testis

  • Park, Min Hee;Park, Ji Eun;Kim, Min Seong;Lee, Kwon Young;Yun, Jung Im;Choi, Jung Hoon;Lee, Eunsong;Lee, Seung Tae
    • Reproductive and Developmental Biology
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    • v.38 no.2
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    • pp.85-91
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    • 2014
  • Despite many researches related with in-vitro culture of porcine spematogonial stem cells (SSCs), adherent culture system widely used has shown a limitation in the maintenance of porcine SSC self-renewal. Therefore, in order to overcome this obstacle, suspension culture, which is known to have numerous advantage over adherent culture, was applied to the culture of porcine SSCs. Porcine SSCs retrieved from neonatal testes were suspension-cultured for 5 days or 20 days, and characteristics of suspension-cultured porcine SSCs including proliferation, alkaline phosphatase (AP) activity, and self-renewal-specific gene expression were investigated and compared with those of adherent-cultured porcine SSCs. As the results, the suspension-cultured porcine SSCs showed entirely non-proliferative and significantly higher rate of AP-positive cells and expression of self-renewal-specific genes than the adherent-cultured porcine SSCs. In addition, long-term culture of porcine SSCs in suspension condition induced significant decrease in the yield of AP staining-positive cells on post-day 10 of culture. These results showed that suspension culture was inappropriate to culture porcine SSCs, because the culture of porcine SSCs in suspension condition didn't stimulate proliferation and maintain AP activity of porcine SSCs, regardless of culture periods.

In Vitro Growth of Bovine Preantral Follicle under Different Culture Conditions

  • Lim, Hyun-Joo;Kim, Dong-Hoon;Im, Gi-Sun;Hwang, Seong-Soo;Baek, Kwang-Soo;Jeon, Byeong-Soon;Park, Sung-Jai;Kim, Hyeon-Shup;Lim, Jeong-Mook
    • Reproductive and Developmental Biology
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    • v.33 no.4
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    • pp.189-194
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    • 2009
  • The objective of this study was to determine effects of different culture media. Preantral follicles were mechanically extracted from bovine ovaries and cultured for 16 days in tissue culture medium (TCM)-199, DMEM or alpha-minimal essential medium ($\alpha$-MEM) + 10% FBS + 0.1 mg/ml sodium pyruvate + 100 mIU/ml FSH. The collected primary follicles from ovary were higher than the primary and secondary follicles. The survival rates of the follicles in TCM-199 were significantly higher (p<0.05) than those in DMEM and $\alpha$-MEM. The diameter of the follicles progressively increased during 12 days of culture. The maximum size ($139.1{\pm}5.4\;{\mu}m$) reached on Day 12 of the in vitro culture and decreased on Day 16. These results suggest that in a culture of bovine preantral follicles, TCM-199 is an optimal medium and a longer-term culture of preantral follicles (>12 days) may be needed to form antra.

Developing the Indicator System for Diagnosing the National Status Quo of Science Culture (국가 수준의 과학문화 실태 진단을 위한 지표 체제 개발)

  • Song, Jin-Woong;Choi, Jae-Hyeok;Kim, Hee-Kyong;Chung, Min-Kyung;Lim, Jin-Young;Cho, Sook-Kyoung
    • Journal of The Korean Association For Science Education
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    • v.28 no.4
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    • pp.316-330
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    • 2008
  • During the past two decades or so, science (or scientific or scientific & technological) culture has become one of the main themes not only of policy makers but also of science educators. Although, the idea of science culture has been taken as a desirable goal, there is little agreement about what it means and how to measure it. Particularly in Korea, there has been a rapid growth of science culture projects and programs, either by governmental or non-governmental, but with little systemic monitoring and evaluation for its practice. The purpose of this study is, thus, to explore a model of measuring science culture and develop a comprehensive indicator system for it. We reviewed many literatures on definitions of science culture and the surveys for related terms, particularly, of recent national and international surveys (e.g. US Science and Engineering Indicators, Eurobarometer, Japanese Science and Technology Indicators). Based on this review, a model for science culture is proposed and then used to define the Science Culture Indicators (SCI). This model encompasses two dimensions(i.e. individual and social), which are further divided into two aspects (i.e. potential and practice). Each dimension is expected to represent citizen literacy of and national infrastructure of science culture respectively. Each category in this $2{\times}2$ matrix is further divided into several sub-categories. The discussion concerning how the model and the indicators can be used to check the states of science culture at social as well as individual levels will be given with some concrete examples, such as indicators particularly related to science education.

A Study on the Scientific Character and Systematization Libraty Science (도서관학의 학문적성격과 체계화에 관한 연구)

  • 김정소
    • Journal of Korean Library and Information Science Society
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    • v.5
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    • pp.1-31
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    • 1978
  • This thesis is to discuss and prove the identity of the acts of the library and the character of the library which regards these as its objects. And this study is analyzed in view of per ceptual problems and scientific problems and scientific problems, in order to make clear the character of the acts of the library. In these processes, some basic propositions are extracted. First, culture can be found in arranged order. Second, human being can create culture by the acquisition of accmulated culture. Third, an individual is purposely related with culture. Fourth, culture is antonomous to mankind. And in this thesis, library science is defined as the systematic formation of mankind's pursuing acts, and to identify this definition, some theoretical models of library science have been offered as follows : Model I 1. Theory of making culture assets informative. 2. Theory of making culture assets empirical; that of imformation treatment. 3. Theory of medial acts of librarians. Model II 4. Theory of organic systems of libraries. Model III 5. Theory of guiding school activities. 6. Theory of extending the inhabitants' interests of communities. 7. Theory of a kind of resources in social improvement. Model IV 8. Theory of a means of principal approach to the succession of culture. In conclusion, this study is to prove that acts of libraries are the universal ones of those human being that are gualified to be the unique means to the creation of culture and, library science is a science that shapes concepts and rules to describe, to make explanation and to foretell these acts.

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Methodology of Lymphocytes Culture for Animal Chromosome Preparation (동물 염색체 분리를 위한 최적 혈액배양 조건에 관한 연구)

  • Sohn, Sea-Hwan;Lee, Jung-Up
    • Reproductive and Developmental Biology
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    • v.35 no.3
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    • pp.257-263
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    • 2011
  • In general, the blood cell culture is a common method for animal chromosome preparation. However, every animal and its cells have unique physiological characteristics and functions. Hence, it is very difficult to find the suitable method of chromosome preparation using animal lymphocyte culture. This study was carried out to fine the suitable method of chromosome preparation using lymphocytes cultures in mammalians and aves including cattle, rat, mouse and chicken. To seek the optimal method of lymphocyte culture in each animal, $2^3$ factorial experiment was designed. The design evaluated three main effects in culture duration, kinds of mitogen supplements and colcemid exposure time with two levels within each effect. The mitotic index and the score of chromosome morphology were analyzed. In results, the suitable methods of lymphocyte culture for chromosome preparation were 72 hours culture, pokeweed mitogen(PWM) supplement and 90 minutes of colcemid exposure in cattle, 72 hours culture, PWM supplement and 50 minutes of colcemid exposure in chicken, 96 hours culture, concanavalin A supplement and 90 minutes of colcemid exposure in rat, and 72 hours culture, PWM supplement and 50 minutes of colcemid exposure in mouse, respectively. In conclusion, kinds of mitogen, culture duration and colcemid exposure time significantly affected the mitotic index and chromosome morphology, in animal lymphocyte culture. The interaction effects between/among treatment factors were also statistically significant.

The Development of the Framework of Science Culture Indicator and Its Application to Secondary School Teachers (과학문화지표체계 개발 및 적용 - 중등교사를 대상으로 -)

  • Kim, Se-Mi;Mun, Kong-Ju;Kim, Sung-Won
    • Journal of The Korean Association For Science Education
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    • v.27 no.9
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    • pp.796-808
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    • 2007
  • There are various definitions of Science Culture nowadays. In this study we redefine Science Culture as a union between Science and Culture. We also develop the Framework of Science Culture Indicator (FSCI). which consists of five fields; History of Science, Philosophy of Science, Literature and Art of Science, Scientific Social Activity and Scientific Media. In this study we also investigated the level of the Science Culture of secondary school teachers, and compare them by teachers' majors: Science, Liberal Arts and Social Studies. To analyze the data, the Kruskal-Wallis Test is adopted. It is found that there are significant differences in the level of Science Culture by teachers' major, and the group of science teachers has the highest level of science culture.